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The map label for this gene is pilS [H]

Identifier: 146283959

GI number: 146283959

Start: 3933752

End: 3935344

Strand: Direct

Name: pilS [H]

Synonym: PST_3645

Alternate gene names: 146283959

Gene position: 3933752-3935344 (Clockwise)

Preceding gene: 146283958

Following gene: 146283960

Centisome position: 86.13

GC content: 65.29

Gene sequence:

>1593_bases
GTGGGCACTGAGAACCTCACCTTCTTCGGCGACCAGGGGCGGCGCATCCTTCGCCTGTATCACCTCTACCGCCTGACCAT
TGGCCTCGCCCTGGTGCTGCTGATCAGCAGCGACCTGCACAACGATCTGCTGCAGATGAGCAATCCGCAGCTGTTTCAGT
ACGGCGCCTGGCTCTATCTGATCCTCAACATCATCGTCGCGGTGCTGGTGCAGAGCCCCGAGCGCGACCTGCCAGTGCTC
AGCCTGGCGCTGATGGACGTGATCCTGCTGTCGGGATTGTTCTATTTTGGCGGAGGAACGCCGAGCGGCATCGGCAACCT
GCTGATCGTCCCCGTCGCCATCGCCAATATTCTCCTGCGGGGGCGGGTCGGTTTCTTCATCGCCGCGGTCGCTGCCACCG
GCCTGATCTACCTGACCTTCTACCTGAGCATCAGCCGTTCCGAAGCCAGCAGCCAGTACGTCCAGGCCGGTGCGCTCGGA
ACGCTGTGCTTCGCCGCAGCCTTTCTCGTCCAGGGACTGACTCGGCGCCTGCAGGTCAGCGAGACCCTGGCCCGCCAGCG
CGCCGAGGAGGTCGCCAACCTCGAGGCCCTCAACGCCCTGATCCTGCAGCGCATGCGTACCGGCATCATGGTGTTGGACC
CGCAGGAGCGCGTGCTCCTGGCCAACCAGGGGGCGCTGCAGTTGCTCGGCCAGGAATCGCTGGCCGGCGAGCGCCTCGCG
CCGCGCTGCCCGGAGCTGCTCAAGGCACTGCAGCACTGGCGTGGCAATCCCACACTGCGCCCGCCGAACCTCAAGGCCAC
CCCCGGCGGACCGACGCTGCAGCCGAGTTTCGCCGCCCTGCAGCACGGCAACAAGCTCGACACTCTGGTGTTCCTCGAGG
ACATCTCGCAGATCGCCCAGAAGGCCCAGCAACTCAAGCTCGCCTCCCTCGGACGACTCACAGCCAGCATTGCCCATGAA
ATCCGCAATCCGCTGGGCGCCATCAGCCATGCTGCTCAGCTGCTACAGGAATCCGAAGAGCTGGACGGCCCGGATCGGCG
ACTGGCGCAGATCATCCAGGACCACTCGCGGCGGATGAATCTGGTCATCGAGAACGTGCTGCAGCTTTCCCGGCGCCGCC
AGGCGGAGCCCCAGCTGCTGGATCTCAAATACTGGCTGCATCGCTTCGTCAGCGAGTTCCGCCAGGGGCTCAGCCCGCAG
CAGACGGTTCACCTGGAAACCATCGGCAGCTCGCTGCAGACGCGCATGGACCCCAACCAGCTGACCCAGGTGCTCACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGGAGCACGATCGCGCTCAGGTCTGGCTCCGCCTGTTTCGCGACGAAG
CCAGCGATCTGCCGGTCGTTGAGGTCCTCGACGATGGGCCCGGCATCGCCGCCGAGCAGTTGCACAACGTTTTCGAACCC
TTCTTCACCACCGAGAGTAAAGGCACCGGGCTCGGGCTGTACATCTCCCGCGAGCTGTGCGAGAGCAACCAGGCACGCCT
GGACTACCGCGAGCGCGCCGAAGGCGGCAGCTGCTTCCGCATCGTCTTCGCGCACCCGCGCAAGCTGAGCTAG

Upstream 100 bases:

>100_bases
TGCGCTCAATGCGGCGTGCACGTGCCGCAGAACCAGGCCCTGCAGGCCCATGACCGCTGGTACTGCAGCCAGGCTCACCT
CGAGCAGGACAGCACCTCTG

Downstream 100 bases:

>100_bases
GCGCGGCAGAACAGTTGCCGGCAACGCATCACACATCGACCAAAGCGGCAGCCACGGCGGCGCCGGGAGAGTTAACATGC
TCGCCAGCCGCCCGGAAGGC

Product: two-component sensor PilS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MGTENLTFFGDQGRRILRLYHLYRLTIGLALVLLISSDLHNDLLQMSNPQLFQYGAWLYLILNIIVAVLVQSPERDLPVL
SLALMDVILLSGLFYFGGGTPSGIGNLLIVPVAIANILLRGRVGFFIAAVAATGLIYLTFYLSISRSEASSQYVQAGALG
TLCFAAAFLVQGLTRRLQVSETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQESLAGERLA
PRCPELLKALQHWRGNPTLRPPNLKATPGGPTLQPSFAALQHGNKLDTLVFLEDISQIAQKAQQLKLASLGRLTASIAHE
IRNPLGAISHAAQLLQESEELDGPDRRLAQIIQDHSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEFRQGLSPQ
QTVHLETIGSSLQTRMDPNQLTQVLTNLVQNGLRYSGKEHDRAQVWLRLFRDEASDLPVVEVLDDGPGIAAEQLHNVFEP
FFTTESKGTGLGLYISRELCESNQARLDYRERAEGGSCFRIVFAHPRKLS

Sequences:

>Translated_530_residues
MGTENLTFFGDQGRRILRLYHLYRLTIGLALVLLISSDLHNDLLQMSNPQLFQYGAWLYLILNIIVAVLVQSPERDLPVL
SLALMDVILLSGLFYFGGGTPSGIGNLLIVPVAIANILLRGRVGFFIAAVAATGLIYLTFYLSISRSEASSQYVQAGALG
TLCFAAAFLVQGLTRRLQVSETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQESLAGERLA
PRCPELLKALQHWRGNPTLRPPNLKATPGGPTLQPSFAALQHGNKLDTLVFLEDISQIAQKAQQLKLASLGRLTASIAHE
IRNPLGAISHAAQLLQESEELDGPDRRLAQIIQDHSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEFRQGLSPQ
QTVHLETIGSSLQTRMDPNQLTQVLTNLVQNGLRYSGKEHDRAQVWLRLFRDEASDLPVVEVLDDGPGIAAEQLHNVFEP
FFTTESKGTGLGLYISRELCESNQARLDYRERAEGGSCFRIVFAHPRKLS
>Mature_529_residues
GTENLTFFGDQGRRILRLYHLYRLTIGLALVLLISSDLHNDLLQMSNPQLFQYGAWLYLILNIIVAVLVQSPERDLPVLS
LALMDVILLSGLFYFGGGTPSGIGNLLIVPVAIANILLRGRVGFFIAAVAATGLIYLTFYLSISRSEASSQYVQAGALGT
LCFAAAFLVQGLTRRLQVSETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQESLAGERLAP
RCPELLKALQHWRGNPTLRPPNLKATPGGPTLQPSFAALQHGNKLDTLVFLEDISQIAQKAQQLKLASLGRLTASIAHEI
RNPLGAISHAAQLLQESEELDGPDRRLAQIIQDHSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEFRQGLSPQQ
TVHLETIGSSLQTRMDPNQLTQVLTNLVQNGLRYSGKEHDRAQVWLRLFRDEASDLPVVEVLDDGPGIAAEQLHNVFEPF
FTTESKGTGLGLYISRELCESNQARLDYRERAEGGSCFRIVFAHPRKLS

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=31.3364055299539, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788549, Length=380, Percent_Identity=25.7894736842105, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=242, Percent_Identity=29.3388429752066, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=28.4518828451883, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=30.6034482758621, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790300, Length=222, Percent_Identity=30.1801801801802, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=25.5144032921811, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=25.6880733944954, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=277, Percent_Identity=25.2707581227437, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=24.3027888446215, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58920; Mature: 58789

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTENLTFFGDQGRRILRLYHLYRLTIGLALVLLISSDLHNDLLQMSNPQLFQYGAWLYL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ILNIIVAVLVQSPERDLPVLSLALMDVILLSGLFYFGGGTPSGIGNLLIVPVAIANILLR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GRVGFFIAAVAATGLIYLTFYLSISRSEASSQYVQAGALGTLCFAAAFLVQGLTRRLQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQESLAGERLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHCCHHHCCCCCC
PRCPELLKALQHWRGNPTLRPPNLKATPGGPTLQPSFAALQHGNKLDTLVFLEDISQIAQ
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
KAQQLKLASLGRLTASIAHEIRNPLGAISHAAQLLQESEELDGPDRRLAQIIQDHSRRMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEFRQGLSPQQTVHLETIGSSLQTRMDPNQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHH
LTQVLTNLVQNGLRYSGKEHDRAQVWLRLFRDEASDLPVVEVLDDGPGIAAEQLHNVFEP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
FFTTESKGTGLGLYISRELCESNQARLDYRERAEGGSCFRIVFAHPRKLS
HHCCCCCCCCEEHEEEHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
GTENLTFFGDQGRRILRLYHLYRLTIGLALVLLISSDLHNDLLQMSNPQLFQYGAWLYL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ILNIIVAVLVQSPERDLPVLSLALMDVILLSGLFYFGGGTPSGIGNLLIVPVAIANILLR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GRVGFFIAAVAATGLIYLTFYLSISRSEASSQYVQAGALGTLCFAAAFLVQGLTRRLQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQESLAGERLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHCCHHHCCCCCC
PRCPELLKALQHWRGNPTLRPPNLKATPGGPTLQPSFAALQHGNKLDTLVFLEDISQIAQ
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
KAQQLKLASLGRLTASIAHEIRNPLGAISHAAQLLQESEELDGPDRRLAQIIQDHSRRMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEFRQGLSPQQTVHLETIGSSLQTRMDPNQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHH
LTQVLTNLVQNGLRYSGKEHDRAQVWLRLFRDEASDLPVVEVLDDGPGIAAEQLHNVFEP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
FFTTESKGTGLGLYISRELCESNQARLDYRERAEGGSCFRIVFAHPRKLS
HHCCCCCCCCEEHEEEHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]