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The map label for this gene is codB [H]

Identifier: 146283886

GI number: 146283886

Start: 3861943

End: 3863220

Strand: Direct

Name: codB [H]

Synonym: PST_3569

Alternate gene names: 146283886

Gene position: 3861943-3863220 (Clockwise)

Preceding gene: 146283883

Following gene: 146283887

Centisome position: 84.55

GC content: 66.74

Gene sequence:

>1278_bases
ATGAAACAGCCCGTCGATACCGACTACCCCCTGAGCGAAGTGCCCGCCGGCGCGCGCAAGGGGCTGTGGTCCACCTCCAT
CCTGCTGTTCGGCTTCACCTTCTTCACCGCCACCATGTTCGCCGGCGGCAAGATCGGCCTGGCCTTCGACTTCAAGACCA
TGCTCTGGGCCGCGGTGATCGGCAACCTGCTGCTCGGCCTCTATGCCGCCGCGCTCGGGCTGATCGCCTGCCGCAGCGGA
CTGAACTCGGTGCTGATGGGGCGCTTCTGCTTTGGCGAAGTGGGCAGCCGGCTGTCCGACTTCCTGCTCGGCTTCACCCA
GATCGGCTGGTACGCCTGGGGCACGGCGACCATCGCCATCGTCCTGGTGCGCCTGCTGGCGTTGCCGGGAAGCTGGACGC
TGCCGTTGATGGTGCTGTTCGGCTTCGGCTTCTGCCTGACCGCCTTCGTCGGCTACAAGGGCCTCGACCTGCTCTCGCGC
ATCGCGGTGCCGGCGATGCTGATCCTGTTGATCGCATCGCTGTGGACCGCCACCCGCGACATTGGCGGGCTGGACGGCCT
GCTCGCGGTACAGCCGGGCGAAAGCCTGACCCTCGGCATGGCCATCACCATGATCTTCGGCACCTTCGTCAGCGGCGCGA
CCCAGGCCACCAACTGGACGCGCTTCGCCCGCAGCAGCAAGGTCGCGGTATGGGCCAGTCTGATCGGCTTCTTCATCGGC
AACGGCCTGATGATCGTCGCCGGCGCCTACGGGGCCATCGTCTACCAGCAGCCGGACATCGTCGAAGTGCTGGTGCTGCA
GGGCCTGTCGATGGCCGCGGTGGTGATGCTCTTTCTCAACCTCTGGACCACGCAGGACAACACCATCTACAACTTCGCCG
CTGCCGGCTGCAACCTGCTGCGCACCGAGCGCCGCCGCCTGGTGACCCTGGGCGGCGCCTTCGTCGGCACGCTGCTGGCG
CTCGGCGGCATGTACGAGTTGCTGATCCCCTTTCTGATCCTGCTCGGCTCGATCATCCCGCCCATCGGCGGCGTGATCAT
GGCCGACTACTTCTACCGCCATCGCGGACAGTACCCGAAGCTGGCCGAGGCGCACCTGCCCAGGTACAACAGCCTGGGCC
TGGCGGCCTATGTGCTGGGTGCCGCCTGCGCCTACTTCTCGCCCTGGGTGGCGCCCATCGTCGGCATACTGGTGGCCGCC
GCAGCCTATATTGTGCTGTACGAGGGCCAGCGCCTGGCCCTGTCGCGCACGGTGCTGACGCAAACCGACGCACGCTGA

Upstream 100 bases:

>100_bases
ACCGCCATCCGACGCGACAGCCCCACTTGGCCGATCGGTCAGCTATGGCATGGCATCTGCACTCCCAGCGCATTCAACCG
GCGCTTGCGGAGCAACCACC

Downstream 100 bases:

>100_bases
TCAAGGAGCCACCATGAACATCCTCAACGCCCGCCTGCGCGGCCGCAGCGGCCTCTACCGCATCGAACTGGACGGCGCGC
GCATCGCCGCCATCAGCGCG

Product: cytosine permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKQPVDTDYPLSEVPAGARKGLWSTSILLFGFTFFTATMFAGGKIGLAFDFKTMLWAAVIGNLLLGLYAAALGLIACRSG
LNSVLMGRFCFGEVGSRLSDFLLGFTQIGWYAWGTATIAIVLVRLLALPGSWTLPLMVLFGFGFCLTAFVGYKGLDLLSR
IAVPAMLILLIASLWTATRDIGGLDGLLAVQPGESLTLGMAITMIFGTFVSGATQATNWTRFARSSKVAVWASLIGFFIG
NGLMIVAGAYGAIVYQQPDIVEVLVLQGLSMAAVVMLFLNLWTTQDNTIYNFAAAGCNLLRTERRRLVTLGGAFVGTLLA
LGGMYELLIPFLILLGSIIPPIGGVIMADYFYRHRGQYPKLAEAHLPRYNSLGLAAYVLGAACAYFSPWVAPIVGILVAA
AAYIVLYEGQRLALSRTVLTQTDAR

Sequences:

>Translated_425_residues
MKQPVDTDYPLSEVPAGARKGLWSTSILLFGFTFFTATMFAGGKIGLAFDFKTMLWAAVIGNLLLGLYAAALGLIACRSG
LNSVLMGRFCFGEVGSRLSDFLLGFTQIGWYAWGTATIAIVLVRLLALPGSWTLPLMVLFGFGFCLTAFVGYKGLDLLSR
IAVPAMLILLIASLWTATRDIGGLDGLLAVQPGESLTLGMAITMIFGTFVSGATQATNWTRFARSSKVAVWASLIGFFIG
NGLMIVAGAYGAIVYQQPDIVEVLVLQGLSMAAVVMLFLNLWTTQDNTIYNFAAAGCNLLRTERRRLVTLGGAFVGTLLA
LGGMYELLIPFLILLGSIIPPIGGVIMADYFYRHRGQYPKLAEAHLPRYNSLGLAAYVLGAACAYFSPWVAPIVGILVAA
AAYIVLYEGQRLALSRTVLTQTDAR
>Mature_425_residues
MKQPVDTDYPLSEVPAGARKGLWSTSILLFGFTFFTATMFAGGKIGLAFDFKTMLWAAVIGNLLLGLYAAALGLIACRSG
LNSVLMGRFCFGEVGSRLSDFLLGFTQIGWYAWGTATIAIVLVRLLALPGSWTLPLMVLFGFGFCLTAFVGYKGLDLLSR
IAVPAMLILLIASLWTATRDIGGLDGLLAVQPGESLTLGMAITMIFGTFVSGATQATNWTRFARSSKVAVWASLIGFFIG
NGLMIVAGAYGAIVYQQPDIVEVLVLQGLSMAAVVMLFLNLWTTQDNTIYNFAAAGCNLLRTERRRLVTLGGAFVGTLLA
LGGMYELLIPFLILLGSIIPPIGGVIMADYFYRHRGQYPKLAEAHLPRYNSLGLAAYVLGAACAYFSPWVAPIVGILVAA
AAYIVLYEGQRLALSRTVLTQTDAR

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=401, Percent_Identity=36.9077306733167, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 45602; Mature: 45602

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPVDTDYPLSEVPAGARKGLWSTSILLFGFTFFTATMFAGGKIGLAFDFKTMLWAAVI
CCCCCCCCCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
GNLLLGLYAAALGLIACRSGLNSVLMGRFCFGEVGSRLSDFLLGFTQIGWYAWGTATIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVRLLALPGSWTLPLMVLFGFGFCLTAFVGYKGLDLLSRIAVPAMLILLIASLWTATRD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGLDGLLAVQPGESLTLGMAITMIFGTFVSGATQATNWTRFARSSKVAVWASLIGFFIG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHC
NGLMIVAGAYGAIVYQQPDIVEVLVLQGLSMAAVVMLFLNLWTTQDNTIYNFAAAGCNLL
CCCEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
RTERRRLVTLGGAFVGTLLALGGMYELLIPFLILLGSIIPPIGGVIMADYFYRHRGQYPK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LAEAHLPRYNSLGLAAYVLGAACAYFSPWVAPIVGILVAAAAYIVLYEGQRLALSRTVLT
HHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QTDAR
CCCCC
>Mature Secondary Structure
MKQPVDTDYPLSEVPAGARKGLWSTSILLFGFTFFTATMFAGGKIGLAFDFKTMLWAAVI
CCCCCCCCCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
GNLLLGLYAAALGLIACRSGLNSVLMGRFCFGEVGSRLSDFLLGFTQIGWYAWGTATIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVRLLALPGSWTLPLMVLFGFGFCLTAFVGYKGLDLLSRIAVPAMLILLIASLWTATRD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGLDGLLAVQPGESLTLGMAITMIFGTFVSGATQATNWTRFARSSKVAVWASLIGFFIG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHC
NGLMIVAGAYGAIVYQQPDIVEVLVLQGLSMAAVVMLFLNLWTTQDNTIYNFAAAGCNLL
CCCEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
RTERRRLVTLGGAFVGTLLALGGMYELLIPFLILLGSIIPPIGGVIMADYFYRHRGQYPK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LAEAHLPRYNSLGLAAYVLGAACAYFSPWVAPIVGILVAAAAYIVLYEGQRLALSRTVLT
HHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QTDAR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]