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The map label for this gene is dnrD [H]

Identifier: 146283835

GI number: 146283835

Start: 3808049

End: 3808732

Strand: Direct

Name: dnrD [H]

Synonym: PST_3518

Alternate gene names: 146283835

Gene position: 3808049-3808732 (Clockwise)

Preceding gene: 146283834

Following gene: 146283836

Centisome position: 83.37

GC content: 62.13

Gene sequence:

>684_bases
ATGGTGCTTCACCGCGTACACCACCAGATCCTCCGCAGCCATCACCTGTTCGAACCGCTCAACGAAGAGCAGATGGAAGA
ACTGCTCAATGCCAGCCAGCTGCTCAACCTGGACAAGGGCGACAACCTGTTCCATCAGGGCGAGCCGGCACACAACTTCT
ACTTCGTCATCTCCGGTGCGGTGAAGGTCTACCGCCTGACACCGGACGGCCAGGAAAAGGTCTTCGAGGTGATCGGCAAC
CGTCAGACCTTCGCCGAAGCCATGATGCTGATGGACACGCCCAACTACGTCGCCTCGGCCCAGGCGGTCTGCCCGTCGCA
GGTCTACCGCTTCTCCAACGCTGCCTACATGCGTCTGCTGGAGGCCAACCAGCGACTGACGTTCGCCCTGCTCGGCAAGC
TCTGCGTGCGCCTGCACCAGCGCATCAACGAGATCGAGACGCTGTCGCTGAAGAACGCCACCCACCGCGTGGTGCGCTAC
CTGCTGACGCAACTGGCGCGGGTGAAGGACGACAGCAACAGCTTCGAACTGCCGATGGCCAAGCAGCTGGTGGCCGGGCA
CCTGTCGATCCAGCCGGAGACGTTCTCACGGATCATCCGCCGACTGATCGACGAGGGCATCATCACCCAGGAAGGTCGGC
AGATCGCCATCCTCGATCGCCAGCGGCTCGAGCAGTTCGAGTGA

Upstream 100 bases:

>100_bases
CCTTGACCGTCATCAACGTCTGGCCTGCGCCATCGCCCAGCACTTGCGCTAGGCTGAAGCCCGACGTTTGTTGTTGTTCA
ATTGAAACAGGACGCCTTGC

Downstream 100 bases:

>100_bases
GTTGATCGCCATCAAAGTCGACGGCGCGAGCAGCGTGTCAGGCTTTGCTGGCGAGGAGGAGCACCACATGCCCAAATGCC
TGTACTGCCAGCAAGACAAT

Product: transcription factor for denitrification gene expression elicited by NO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MVLHRVHHQILRSHHLFEPLNEEQMEELLNASQLLNLDKGDNLFHQGEPAHNFYFVISGAVKVYRLTPDGQEKVFEVIGN
RQTFAEAMMLMDTPNYVASAQAVCPSQVYRFSNAAYMRLLEANQRLTFALLGKLCVRLHQRINEIETLSLKNATHRVVRY
LLTQLARVKDDSNSFELPMAKQLVAGHLSIQPETFSRIIRRLIDEGIITQEGRQIAILDRQRLEQFE

Sequences:

>Translated_227_residues
MVLHRVHHQILRSHHLFEPLNEEQMEELLNASQLLNLDKGDNLFHQGEPAHNFYFVISGAVKVYRLTPDGQEKVFEVIGN
RQTFAEAMMLMDTPNYVASAQAVCPSQVYRFSNAAYMRLLEANQRLTFALLGKLCVRLHQRINEIETLSLKNATHRVVRY
LLTQLARVKDDSNSFELPMAKQLVAGHLSIQPETFSRIIRRLIDEGIITQEGRQIAILDRQRLEQFE
>Mature_227_residues
MVLHRVHHQILRSHHLFEPLNEEQMEELLNASQLLNLDKGDNLFHQGEPAHNFYFVISGAVKVYRLTPDGQEKVFEVIGN
RQTFAEAMMLMDTPNYVASAQAVCPSQVYRFSNAAYMRLLEANQRLTFALLGKLCVRLHQRINEIETLSLKNATHRVVRY
LLTQLARVKDDSNSFELPMAKQLVAGHLSIQPETFSRIIRRLIDEGIITQEGRQIAILDRQRLEQFE

Specific function: Transcriptional activator of anaerobic gene expression [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=205, Percent_Identity=21.4634146341463, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26236; Mature: 26236

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLHRVHHQILRSHHLFEPLNEEQMEELLNASQLLNLDKGDNLFHQGEPAHNFYFVISGA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
VKVYRLTPDGQEKVFEVIGNRQTFAEAMMLMDTPNYVASAQAVCPSQVYRFSNAAYMRLL
EEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCHHHHHHH
EANQRLTFALLGKLCVRLHQRINEIETLSLKNATHRVVRYLLTQLARVKDDSNSFELPMA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KQLVAGHLSIQPETFSRIIRRLIDEGIITQEGRQIAILDRQRLEQFE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHCC
>Mature Secondary Structure
MVLHRVHHQILRSHHLFEPLNEEQMEELLNASQLLNLDKGDNLFHQGEPAHNFYFVISGA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
VKVYRLTPDGQEKVFEVIGNRQTFAEAMMLMDTPNYVASAQAVCPSQVYRFSNAAYMRLL
EEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCHHHHHHH
EANQRLTFALLGKLCVRLHQRINEIETLSLKNATHRVVRYLLTQLARVKDDSNSFELPMA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KQLVAGHLSIQPETFSRIIRRLIDEGIITQEGRQIAILDRQRLEQFE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1787797; 1787798; 10984043 [H]