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The map label for this gene is hflB [C]

Identifier: 146283772

GI number: 146283772

Start: 3740101

End: 3742281

Strand: Direct

Name: hflB [C]

Synonym: PST_3455

Alternate gene names: 146283772

Gene position: 3740101-3742281 (Clockwise)

Preceding gene: 146283771

Following gene: 146283773

Centisome position: 81.89

GC content: 53.97

Gene sequence:

>2181_bases
ATGTCGAAATCCGCTCGCAAATCTGGCCCACACAACCAGGACACATGCCCGCTTGCTCTGAGCTGGATGTTTAAGCTGAT
GATTGATTGCGGCGGCTATCGGGATTTCATCAATCACGGTTGCTTCAGTGATGACGATGTCGCTCGCGAGCTTGGGATCT
ACTGGCTAGTAGACGCTGCTGAGAGTGGTGAAGAAGATGAGGACGAATCCCGTTTCCCCAGTACTCTGCATGGTAGAGCT
AGACGCTCGAACGTCGCCAGAAAACCCAGCTTTAAGGAGCGATCACTGGCTGAGCTGCGCAAAGAGCGCAAGCTCTTCGA
CAAAGCCTTTCCTTCCCCAGAACTACCAGACAACCTTCAGGCGAATCTGACAAAACTTGCACAGCTGATTGGCCTGGATG
AGATCGAGCAGAAGCTGATCGGCTTCTGCTCGGTGATGAATACAGACACCCTGCTGGATGACTGTTGCAATGAACTCGGT
CACATAGGCTTTAACCGCCTTATCCGAGTGCTGAGCACATTGTTGGACATCCCGCAGAATGACCTAAGGTTGCGGCTTTC
GGCAGAGGGCCGCCTGGTGCAGTCAGGTCTAATTGAAGCAAACCTGCGCAGCGGCGTGCGCAACCATATGTCCGACCTGC
TTGGCTTTAACAACAAGGATCTGCTGTTTTCCGTGCGTCACCACCAAGGTAGCGCTATCGGTTTGTTCCAGAGTGCCTTT
CGCCCTGCACCCGAAAGCATGCTTGAAGCCAAAGACTTTATTCACCTCGACACCCCGCTCAACATTACCCGCCCCTACCT
GCAGCAAGCACTCGACGCGAAAAAAACCGGGGTTAACATCCTCATCTACGGCCCACCCGGCACCGGCAAGAGCCAGCTCA
CACGCCTGCTTGCGAGCGAACTACAAGCTGAACTCTTTGAAATTGCCTGCACTGATAACAGTGGTGATCCGATTGATCGG
CGCGGACGCCTTTGCGCCTTAAGGTCGGCCATGTGCCTACTCAACCAGCAGAAAACGTTGCTGGTGCTGGATGAGATCGA
GGATCTATTCAGTGGCAGCAATGAAATGGCCATGTTCATGGGTGGGCAAGAACGGCAAAAAGGCTGGATTAACCGCATGC
TTGAGGAGAATCCAATCCCCTGCTTCTGGCTGACCAACAACATCAGGGCCTTGGATAATGCCTATATCCGGCGTTTCGAC
CTTGTGCTCAAGTTGGAAAATCCACCTCGGCTTCAGCGTGAAGGCATTATCCGCAGCGCCAGTGACAATCGCCTGACTCA
GTCGCTAGTCGAAAAGCTCGCTGATCACGAACAGCTGATGCCCGCCATCATCACGCGAGCCATGGACGTTGCAAACAGCC
AGGCTCAAGACGCATCTGCGACCTTGGACACGACAGTCGAATTTCTGGTCAACGCCACACTGCAGGCCCAAGGGTTTGCG
CGCCTAGGGCAGAATCGTATGCAGGCATTGCCTGAGTTCTACAGCCCAAACTTGGCCAATACCAACATGCCTCTCGACCA
ACTGCTTACTGGGTTGCGCAATCATGGTGAGGCGCGCCTGTGCTTTTATGGCCCACCGGGCACAGGTAAGACTGCGTTTG
GTCATTGGTTGGCGATTCAACTTGGTAAGCCGCTTCTGACTCGTCGGGCATCCGACCTCATCTCGCCTTACGTCGGCATG
ACCGAGCAGAACTTCGCCCGAGCATTTGAGCAGGCCACCGATGATGATGCCGTCTTGCTTCTCGATGAAGTTGACAGCTT
CCTGCAAGACCGTCGAAAGGCACGCCACAGCTGGGAAGTAAGCGTGGTAAACGAAATGCTGACGCAGATGGAGAGCTATC
GCGGGCTATTTATAGCTTCCACAAACCTCATGGAAAACCTCGACGAGGCCTCACTGCGACGGTTTGATTTGAAAATTAAT
TTCGACAACCTCGGCGCTGAACAGATCACCAACCTGCTTCGACAGTACCTGCGGCAAATGGGCCTCAAAAAGCCAGACAG
CCTCAAGCTCGCGCGATTACTTCGGCAAAGCTGCCTGACCCCAGGTGACTTTGCACTGGTCGCACGACGTGCACGCTTCC
AACCATTCACCAATGCCGATCAGGTGGTTGATGCGTTACTGGCCGAAAGCAGCTTAAAAACCGCCACTACCATGAGAGCG
ATTGGTTTTGCTGAGTGGTAG

Upstream 100 bases:

>100_bases
GGAGAGCTGGAACCCTAACTCCCCTACCTCCGCACAGCTTGCGACGCATTACGTCGCAAGCATCCTCCACAATGCCTCCA
CATTCTCTGGAGGACTTAAA

Downstream 100 bases:

>100_bases
AACGTTGCCCCAAGGTCATCTTCAAACACCCAACAAAGCAGATCGGTTAAGCGGCAAGGCAGGAGTGCGAAATGGTTGAT
GCAGCGGCGGGAACATCCCA

Product: ATPase of the AAA+ class

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSKSARKSGPHNQDTCPLALSWMFKLMIDCGGYRDFINHGCFSDDDVARELGIYWLVDAAESGEEDEDESRFPSTLHGRA
RRSNVARKPSFKERSLAELRKERKLFDKAFPSPELPDNLQANLTKLAQLIGLDEIEQKLIGFCSVMNTDTLLDDCCNELG
HIGFNRLIRVLSTLLDIPQNDLRLRLSAEGRLVQSGLIEANLRSGVRNHMSDLLGFNNKDLLFSVRHHQGSAIGLFQSAF
RPAPESMLEAKDFIHLDTPLNITRPYLQQALDAKKTGVNILIYGPPGTGKSQLTRLLASELQAELFEIACTDNSGDPIDR
RGRLCALRSAMCLLNQQKTLLVLDEIEDLFSGSNEMAMFMGGQERQKGWINRMLEENPIPCFWLTNNIRALDNAYIRRFD
LVLKLENPPRLQREGIIRSASDNRLTQSLVEKLADHEQLMPAIITRAMDVANSQAQDASATLDTTVEFLVNATLQAQGFA
RLGQNRMQALPEFYSPNLANTNMPLDQLLTGLRNHGEARLCFYGPPGTGKTAFGHWLAIQLGKPLLTRRASDLISPYVGM
TEQNFARAFEQATDDDAVLLLDEVDSFLQDRRKARHSWEVSVVNEMLTQMESYRGLFIASTNLMENLDEASLRRFDLKIN
FDNLGAEQITNLLRQYLRQMGLKKPDSLKLARLLRQSCLTPGDFALVARRARFQPFTNADQVVDALLAESSLKTATTMRA
IGFAEW

Sequences:

>Translated_726_residues
MSKSARKSGPHNQDTCPLALSWMFKLMIDCGGYRDFINHGCFSDDDVARELGIYWLVDAAESGEEDEDESRFPSTLHGRA
RRSNVARKPSFKERSLAELRKERKLFDKAFPSPELPDNLQANLTKLAQLIGLDEIEQKLIGFCSVMNTDTLLDDCCNELG
HIGFNRLIRVLSTLLDIPQNDLRLRLSAEGRLVQSGLIEANLRSGVRNHMSDLLGFNNKDLLFSVRHHQGSAIGLFQSAF
RPAPESMLEAKDFIHLDTPLNITRPYLQQALDAKKTGVNILIYGPPGTGKSQLTRLLASELQAELFEIACTDNSGDPIDR
RGRLCALRSAMCLLNQQKTLLVLDEIEDLFSGSNEMAMFMGGQERQKGWINRMLEENPIPCFWLTNNIRALDNAYIRRFD
LVLKLENPPRLQREGIIRSASDNRLTQSLVEKLADHEQLMPAIITRAMDVANSQAQDASATLDTTVEFLVNATLQAQGFA
RLGQNRMQALPEFYSPNLANTNMPLDQLLTGLRNHGEARLCFYGPPGTGKTAFGHWLAIQLGKPLLTRRASDLISPYVGM
TEQNFARAFEQATDDDAVLLLDEVDSFLQDRRKARHSWEVSVVNEMLTQMESYRGLFIASTNLMENLDEASLRRFDLKIN
FDNLGAEQITNLLRQYLRQMGLKKPDSLKLARLLRQSCLTPGDFALVARRARFQPFTNADQVVDALLAESSLKTATTMRA
IGFAEW
>Mature_725_residues
SKSARKSGPHNQDTCPLALSWMFKLMIDCGGYRDFINHGCFSDDDVARELGIYWLVDAAESGEEDEDESRFPSTLHGRAR
RSNVARKPSFKERSLAELRKERKLFDKAFPSPELPDNLQANLTKLAQLIGLDEIEQKLIGFCSVMNTDTLLDDCCNELGH
IGFNRLIRVLSTLLDIPQNDLRLRLSAEGRLVQSGLIEANLRSGVRNHMSDLLGFNNKDLLFSVRHHQGSAIGLFQSAFR
PAPESMLEAKDFIHLDTPLNITRPYLQQALDAKKTGVNILIYGPPGTGKSQLTRLLASELQAELFEIACTDNSGDPIDRR
GRLCALRSAMCLLNQQKTLLVLDEIEDLFSGSNEMAMFMGGQERQKGWINRMLEENPIPCFWLTNNIRALDNAYIRRFDL
VLKLENPPRLQREGIIRSASDNRLTQSLVEKLADHEQLMPAIITRAMDVANSQAQDASATLDTTVEFLVNATLQAQGFAR
LGQNRMQALPEFYSPNLANTNMPLDQLLTGLRNHGEARLCFYGPPGTGKTAFGHWLAIQLGKPLLTRRASDLISPYVGMT
EQNFARAFEQATDDDAVLLLDEVDSFLQDRRKARHSWEVSVVNEMLTQMESYRGLFIASTNLMENLDEASLRRFDLKINF
DNLGAEQITNLLRQYLRQMGLKKPDSLKLARLLRQSCLTPGDFALVARRARFQPFTNADQVVDALLAESSLKTATTMRAI
GFAEW

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Homo sapiens, GI45643125, Length=150, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI31742536, Length=135, Percent_Identity=35.5555555555556, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI112789543, Length=135, Percent_Identity=35.5555555555556, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI45643123, Length=150, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI11875211, Length=136, Percent_Identity=33.8235294117647, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI40806170, Length=136, Percent_Identity=33.8235294117647, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI6005942, Length=150, Percent_Identity=28.6666666666667, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789568, Length=160, Percent_Identity=31.875, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17531535, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17537217, Length=200, Percent_Identity=29.5, Blast_Score=70, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17532375, Length=427, Percent_Identity=25.2927400468384, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320077, Length=150, Percent_Identity=31.3333333333333, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321465, Length=178, Percent_Identity=27.5280898876405, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324000, Length=152, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI161076486, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076488, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137560, Length=217, Percent_Identity=27.6497695852535, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571849, Length=207, Percent_Identity=31.4009661835749, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571847, Length=207, Percent_Identity=31.4009661835749, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355121, Length=459, Percent_Identity=25.7080610021786, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 81672; Mature: 81541

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSARKSGPHNQDTCPLALSWMFKLMIDCGGYRDFINHGCFSDDDVARELGIYWLVDAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCEEEEECC
ESGEEDEDESRFPSTLHGRARRSNVARKPSFKERSLAELRKERKLFDKAFPSPELPDNLQ
CCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ANLTKLAQLIGLDEIEQKLIGFCSVMNTDTLLDDCCNELGHIGFNRLIRVLSTLLDIPQN
HHHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
DLRLRLSAEGRLVQSGLIEANLRSGVRNHMSDLLGFNNKDLLFSVRHHQGSAIGLFQSAF
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHC
RPAPESMLEAKDFIHLDTPLNITRPYLQQALDAKKTGVNILIYGPPGTGKSQLTRLLASE
CCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
LQAELFEIACTDNSGDPIDRRGRLCALRSAMCLLNQQKTLLVLDEIEDLFSGSNEMAMFM
HHHHHHHEEECCCCCCCCHHCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEE
GGQERQKGWINRMLEENPIPCFWLTNNIRALDNAYIRRFDLVLKLENPPRLQREGIIRSA
CCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCC
SDNRLTQSLVEKLADHEQLMPAIITRAMDVANSQAQDASATLDTTVEFLVNATLQAQGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLGQNRMQALPEFYSPNLANTNMPLDQLLTGLRNHGEARLCFYGPPGTGKTAFGHWLAIQ
HHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHEEEH
LGKPLLTRRASDLISPYVGMTEQNFARAFEQATDDDAVLLLDEVDSFLQDRRKARHSWEV
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCHH
SVVNEMLTQMESYRGLFIASTNLMENLDEASLRRFDLKINFDNLGAEQITNLLRQYLRQM
HHHHHHHHHHHHHCCEEEEECHHHHCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHC
GLKKPDSLKLARLLRQSCLTPGDFALVARRARFQPFTNADQVVDALLAESSLKTATTMRA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGFAEW
HCCCCC
>Mature Secondary Structure 
SKSARKSGPHNQDTCPLALSWMFKLMIDCGGYRDFINHGCFSDDDVARELGIYWLVDAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCEEEEECC
ESGEEDEDESRFPSTLHGRARRSNVARKPSFKERSLAELRKERKLFDKAFPSPELPDNLQ
CCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ANLTKLAQLIGLDEIEQKLIGFCSVMNTDTLLDDCCNELGHIGFNRLIRVLSTLLDIPQN
HHHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
DLRLRLSAEGRLVQSGLIEANLRSGVRNHMSDLLGFNNKDLLFSVRHHQGSAIGLFQSAF
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHC
RPAPESMLEAKDFIHLDTPLNITRPYLQQALDAKKTGVNILIYGPPGTGKSQLTRLLASE
CCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
LQAELFEIACTDNSGDPIDRRGRLCALRSAMCLLNQQKTLLVLDEIEDLFSGSNEMAMFM
HHHHHHHEEECCCCCCCCHHCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEE
GGQERQKGWINRMLEENPIPCFWLTNNIRALDNAYIRRFDLVLKLENPPRLQREGIIRSA
CCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCC
SDNRLTQSLVEKLADHEQLMPAIITRAMDVANSQAQDASATLDTTVEFLVNATLQAQGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLGQNRMQALPEFYSPNLANTNMPLDQLLTGLRNHGEARLCFYGPPGTGKTAFGHWLAIQ
HHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHEEEH
LGKPLLTRRASDLISPYVGMTEQNFARAFEQATDDDAVLLLDEVDSFLQDRRKARHSWEV
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCHH
SVVNEMLTQMESYRGLFIASTNLMENLDEASLRRFDLKINFDNLGAEQITNLLRQYLRQM
HHHHHHHHHHHHHCCEEEEECHHHHCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHC
GLKKPDSLKLARLLRQSCLTPGDFALVARRARFQPFTNADQVVDALLAESSLKTATTMRA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGFAEW
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]