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The map label for this gene is pykF [H]

Identifier: 146283723

GI number: 146283723

Start: 3679180

End: 3681057

Strand: Direct

Name: pykF [H]

Synonym: PST_3406

Alternate gene names: 146283723

Gene position: 3679180-3681057 (Clockwise)

Preceding gene: 146283722

Following gene: 146283724

Centisome position: 80.55

GC content: 59.0

Gene sequence:

>1878_bases
ATGTCGAAAAAGGCACTGACCCCGCCCTCTGCGGCCTTGCATTTCAATCCATTACGCGTGCAACTCGAACGGCTACGTAA
CGAGATGACGAGCGACGCCTGCCTGGCATCGCTGAAAAATATCCAGGTTCATGAGTGCTACAGGCAAAGCGCCGAGAACC
TGCTGCAGTACCTATGTCTTCGTCGCCATGACCTGCGCGAGTTACAAGCGCAGCTTGCCGAGATCGGTTTGTCGTCCTTG
GGCCGCGCCGAGGCGGCAATACTCGCAGCTGTGACATCGGTGATGGGCGTGTTGCAGCGGCTCGAAGAGCCGAATACCCA
AGAGCCCAAGGCCCCTGAGCGTGGTCTCCACGAGGGTGAGCGTCTATTGGCGGCTCACGCCGACGCGCTGTTTGGAAAGG
CCCGTGGCACGCGCTCGATTCGAATCATGGTCACCCTCCCCTCTGAAGCGGCGCACAACCGCGACCTCATCGAAGCGTTG
ATCAAGGAAGGCATGGATTGCGCGCGCATCAACTGCGCACACGATGACCCAGACAGCTGGCGTGCGATGATCGAGCATGT
TCGTGCAGCGGAGAAAGCGCTTGGGCGCGAATGCAAGGTAGCGCTCGATCTCGCTGGGCCCAAGTTACGCACAGGCCCTA
TCGCAACGGCGCCAGGTGTAAAAAAGGTACGGCCCACGCGTGATTGCTATGGGCAAGTCATCAGCCCCGCCCACGTTCGT
ATTACCTGGGCGACGGCAGCCATAAACGAGTCCGATACCGACCCATGCTTGATGGTTACAAGCGATAAAGCGCTGGCAGT
CCGTGCTGGCGATACGCTTAGGTTACGCGATGCGCGCGGCTCGAAACGAAAGATGAAGGTCATCGCGTCTGATGAGGAGG
GGTGCCTGGCGGAGCTGAAGAAAACGGCCTACTTCACAGACGGCAGGCAGATCAAACAGCGTCGGGCAGGCAAGACACTG
GGACGAATGCGCGTTACTGGCTTGCCGCCGCGCGAGCAGCATTTGACCCTGCGGGTTGGCGACCTGTTGGCGCTGACCGC
TGACGACCAACCTATCGATCCACCGAGCAACGACAATGCCGAGACCGCAAGGATAGGCTGCACACTACCGCACGTATTGG
CGGCGGTCGCAGCGGGCGACCCTGTTTGGTTCGACGATGGCAAAATTGGGGCGCGGGTGGAGAAAGCGTCTGCCGATGCG
CTGATCTTGCGCATTACCCAAATCGCACATGCAAGCGGCCGCGCCAAGCTGGCAAGTGACAAGGGCATCAACTTCCCAGA
CAACGCCCTCCCAGTCCGAGCACCGACCGAGGACGATATTGAAACACTGGCGTTTGCGGCGAAACACGCCGATATCGTTC
AGATGTCGTTCGCAAACAGCGCCGAAGACGTGATTGAATTGATTGATCATTTGGAACGACTGGACGCGACGCACCTTGGA
GTAGTGTTGAAAATCGAGACCCGTGCCGGATTCGAAAACCTGCCCAAGATGCTGCTCGCCGGCATGCGATTGCCACGATT
TGGCGTCATGATCGCCCGGGGCGACCTGGCCGTAGAGACCGGTTTCGAACGTTTGGCCGAAATTCAGGAAGAGATGCTGT
GCCTGTGTGAGGCAGCCCATGTACCAGTGATATGGGCAACCCAGGTTCTTGAAAGCCTGGCTAAGAAGGGAGCGCCGGCG
CGCTCAGAAATTACCGATGCAGCCATGAGCGTTCGTGCTGAGTGCGTAATGCTCAACAAGGGGCCATACATTCTTAAGGC
GGTGACAACCCTCAATGACGTGCTGCGCCGCATGCGGGAGCATCGCGCGAAGAAACGGGACTTACTTCGCTCGTTGCAAG
TGGCTGGGCGGCAGGATGCAACCGGGGCGCTAAGCTGA

Upstream 100 bases:

>100_bases
GACGACCCACTGGTCGGCTGTCATGAAACAGGTCCAACCTGCATCAGGTCAGTGTATACCGGTAGCGGCCGGTCGGGCCG
CAGGCTCTCTGGAGATTTCG

Downstream 100 bases:

>100_bases
AGGCTGCTGAAGGAAGGTGTCGCGGTCACTGGCAATGGTGTTCAGCAGTGCTTTTCTTCGAGTTATCACCCGTCTGCCAC
TCGTATCTGCAGGCCCCATC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSKKALTPPSAALHFNPLRVQLERLRNEMTSDACLASLKNIQVHECYRQSAENLLQYLCLRRHDLRELQAQLAEIGLSSL
GRAEAAILAAVTSVMGVLQRLEEPNTQEPKAPERGLHEGERLLAAHADALFGKARGTRSIRIMVTLPSEAAHNRDLIEAL
IKEGMDCARINCAHDDPDSWRAMIEHVRAAEKALGRECKVALDLAGPKLRTGPIATAPGVKKVRPTRDCYGQVISPAHVR
ITWATAAINESDTDPCLMVTSDKALAVRAGDTLRLRDARGSKRKMKVIASDEEGCLAELKKTAYFTDGRQIKQRRAGKTL
GRMRVTGLPPREQHLTLRVGDLLALTADDQPIDPPSNDNAETARIGCTLPHVLAAVAAGDPVWFDDGKIGARVEKASADA
LILRITQIAHASGRAKLASDKGINFPDNALPVRAPTEDDIETLAFAAKHADIVQMSFANSAEDVIELIDHLERLDATHLG
VVLKIETRAGFENLPKMLLAGMRLPRFGVMIARGDLAVETGFERLAEIQEEMLCLCEAAHVPVIWATQVLESLAKKGAPA
RSEITDAAMSVRAECVMLNKGPYILKAVTTLNDVLRRMREHRAKKRDLLRSLQVAGRQDATGALS

Sequences:

>Translated_625_residues
MSKKALTPPSAALHFNPLRVQLERLRNEMTSDACLASLKNIQVHECYRQSAENLLQYLCLRRHDLRELQAQLAEIGLSSL
GRAEAAILAAVTSVMGVLQRLEEPNTQEPKAPERGLHEGERLLAAHADALFGKARGTRSIRIMVTLPSEAAHNRDLIEAL
IKEGMDCARINCAHDDPDSWRAMIEHVRAAEKALGRECKVALDLAGPKLRTGPIATAPGVKKVRPTRDCYGQVISPAHVR
ITWATAAINESDTDPCLMVTSDKALAVRAGDTLRLRDARGSKRKMKVIASDEEGCLAELKKTAYFTDGRQIKQRRAGKTL
GRMRVTGLPPREQHLTLRVGDLLALTADDQPIDPPSNDNAETARIGCTLPHVLAAVAAGDPVWFDDGKIGARVEKASADA
LILRITQIAHASGRAKLASDKGINFPDNALPVRAPTEDDIETLAFAAKHADIVQMSFANSAEDVIELIDHLERLDATHLG
VVLKIETRAGFENLPKMLLAGMRLPRFGVMIARGDLAVETGFERLAEIQEEMLCLCEAAHVPVIWATQVLESLAKKGAPA
RSEITDAAMSVRAECVMLNKGPYILKAVTTLNDVLRRMREHRAKKRDLLRSLQVAGRQDATGALS
>Mature_624_residues
SKKALTPPSAALHFNPLRVQLERLRNEMTSDACLASLKNIQVHECYRQSAENLLQYLCLRRHDLRELQAQLAEIGLSSLG
RAEAAILAAVTSVMGVLQRLEEPNTQEPKAPERGLHEGERLLAAHADALFGKARGTRSIRIMVTLPSEAAHNRDLIEALI
KEGMDCARINCAHDDPDSWRAMIEHVRAAEKALGRECKVALDLAGPKLRTGPIATAPGVKKVRPTRDCYGQVISPAHVRI
TWATAAINESDTDPCLMVTSDKALAVRAGDTLRLRDARGSKRKMKVIASDEEGCLAELKKTAYFTDGRQIKQRRAGKTLG
RMRVTGLPPREQHLTLRVGDLLALTADDQPIDPPSNDNAETARIGCTLPHVLAAVAAGDPVWFDDGKIGARVEKASADAL
ILRITQIAHASGRAKLASDKGINFPDNALPVRAPTEDDIETLAFAAKHADIVQMSFANSAEDVIELIDHLERLDATHLGV
VLKIETRAGFENLPKMLLAGMRLPRFGVMIARGDLAVETGFERLAEIQEEMLCLCEAAHVPVIWATQVLESLAKKGAPAR
SEITDAAMSVRAECVMLNKGPYILKAVTTLNDVLRRMREHRAKKRDLLRSLQVAGRQDATGALS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=251, Percent_Identity=33.4661354581673, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI33286420, Length=251, Percent_Identity=33.4661354581673, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI33286418, Length=225, Percent_Identity=35.1111111111111, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI10835121, Length=269, Percent_Identity=29.7397769516729, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI32967597, Length=269, Percent_Identity=29.7397769516729, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI310128732, Length=178, Percent_Identity=31.4606741573034, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310128730, Length=178, Percent_Identity=31.4606741573034, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1787965, Length=270, Percent_Identity=30, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788160, Length=269, Percent_Identity=27.1375464684015, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17544584, Length=251, Percent_Identity=32.2709163346614, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71984413, Length=271, Percent_Identity=29.520295202952, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506829, Length=271, Percent_Identity=29.520295202952, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506831, Length=271, Percent_Identity=29.520295202952, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71984406, Length=271, Percent_Identity=29.520295202952, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319279, Length=228, Percent_Identity=31.140350877193, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324923, Length=224, Percent_Identity=30.8035714285714, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571814, Length=251, Percent_Identity=32.2709163346614, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648964, Length=251, Percent_Identity=32.2709163346614, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648966, Length=267, Percent_Identity=29.5880149812734, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24581235, Length=298, Percent_Identity=25.8389261744966, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 68267; Mature: 68135

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKALTPPSAALHFNPLRVQLERLRNEMTSDACLASLKNIQVHECYRQSAENLLQYLCL
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RRHDLRELQAQLAEIGLSSLGRAEAAILAAVTSVMGVLQRLEEPNTQEPKAPERGLHEGE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHH
RLLAAHADALFGKARGTRSIRIMVTLPSEAAHNRDLIEALIKEGMDCARINCAHDDPDSW
HHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
RAMIEHVRAAEKALGRECKVALDLAGPKLRTGPIATAPGVKKVRPTRDCYGQVISPAHVR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEE
ITWATAAINESDTDPCLMVTSDKALAVRAGDTLRLRDARGSKRKMKVIASDEEGCLAELK
EEEEEEECCCCCCCCEEEEECCCEEEEECCCEEEEECCCCCCCEEEEEECCCCHHHHHHH
KTAYFTDGRQIKQRRAGKTLGRMRVTGLPPREQHLTLRVGDLLALTADDQPIDPPSNDNA
HHHCCCCCHHHHHHHHCCHHHCEEECCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCC
ETARIGCTLPHVLAAVAAGDPVWFDDGKIGARVEKASADALILRITQIAHASGRAKLASD
CCEEECCCHHHHHHHHHCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEECCC
KGINFPDNALPVRAPTEDDIETLAFAAKHADIVQMSFANSAEDVIELIDHLERLDATHLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHEEEHHCCCHHHHHHHHHHHHHHCHHHCC
VVLKIETRAGFENLPKMLLAGMRLPRFGVMIARGDLAVETGFERLAEIQEEMLCLCEAAH
EEEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
VPVIWATQVLESLAKKGAPARSEITDAAMSVRAECVMLNKGPYILKAVTTLNDVLRRMRE
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHH
HRAKKRDLLRSLQVAGRQDATGALS
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKKALTPPSAALHFNPLRVQLERLRNEMTSDACLASLKNIQVHECYRQSAENLLQYLCL
CCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RRHDLRELQAQLAEIGLSSLGRAEAAILAAVTSVMGVLQRLEEPNTQEPKAPERGLHEGE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHH
RLLAAHADALFGKARGTRSIRIMVTLPSEAAHNRDLIEALIKEGMDCARINCAHDDPDSW
HHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
RAMIEHVRAAEKALGRECKVALDLAGPKLRTGPIATAPGVKKVRPTRDCYGQVISPAHVR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEE
ITWATAAINESDTDPCLMVTSDKALAVRAGDTLRLRDARGSKRKMKVIASDEEGCLAELK
EEEEEEECCCCCCCCEEEEECCCEEEEECCCEEEEECCCCCCCEEEEEECCCCHHHHHHH
KTAYFTDGRQIKQRRAGKTLGRMRVTGLPPREQHLTLRVGDLLALTADDQPIDPPSNDNA
HHHCCCCCHHHHHHHHCCHHHCEEECCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCC
ETARIGCTLPHVLAAVAAGDPVWFDDGKIGARVEKASADALILRITQIAHASGRAKLASD
CCEEECCCHHHHHHHHHCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEECCC
KGINFPDNALPVRAPTEDDIETLAFAAKHADIVQMSFANSAEDVIELIDHLERLDATHLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHEEEHHCCCHHHHHHHHHHHHHHCHHHCC
VVLKIETRAGFENLPKMLLAGMRLPRFGVMIARGDLAVETGFERLAEIQEEMLCLCEAAH
EEEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
VPVIWATQVLESLAKKGAPARSEITDAAMSVRAECVMLNKGPYILKAVTTLNDVLRRMRE
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHH
HRAKKRDLLRSLQVAGRQDATGALS
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]