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The map label for this gene is rlmL [H]

Identifier: 146283621

GI number: 146283621

Start: 3573430

End: 3574290

Strand: Direct

Name: rlmL [H]

Synonym: PST_3301

Alternate gene names: 146283621

Gene position: 3573430-3574290 (Clockwise)

Preceding gene: 146283620

Following gene: 146283622

Centisome position: 78.24

GC content: 67.36

Gene sequence:

>861_bases
ATGGTGCAGAGCTTTCACCAGACGCTTGCAGCGGATGCGTTGCTGCGCATCCATGAGCTGGCCATGGATGCGCTCGAACT
GCCGCTGCAGCTCGTCTACAACGACCGCTCGCAGGGCAACTCGCGCATCGACCGCAGCCACGCGGCGTACCAGCCGGACG
CCGCCGCGCTGGCCGATCAGGTCGGTCATGAATGGGGGCTCGCCTATCGCGTACGCGGGCGCCACGACGGCCAGGACCCG
CTGCTGTTCCTCGACCTGCGCAACGCTCGCGGCTGGGTCAAGCAACACAGTGCCGGCAAGTCGGTGCTCAACCTGTTTTC
CTATACCTGCGGCGTCGGGCTCTGCGCTGCGGCGGGTGGCGCCCGGGATGTCTGGAACCTCGACTTCGCCGAGCGCAACC
TCGCCGTGGGCAGGGAGAATGCAGCGCTGAACCCGGGGCTGGCACCGATGCGCTTCGTCCGCTCCGACTATTTCCCGGCG
ATCCGCCAACTCGCCGGACTGCCGGTGGCTTCCAGGCGCGGCCGCGCCCTGCCCGACTACCCTCGCCTCGAGCAGCGCCA
GTTCGATCTGGTACTGCTCGACCCTCCGGCCTGGGCACGCAGCGCCTTCGGCACGGTGGACCTGCTGCGCGACTACCAGA
GCCTGCTGAAACCGGCATTGCTGACCACCGCCGAAGGCGGCGTGCTGATCTGTTGCAACAACCTGGCGAAGGTGCCGATG
GACGAATGGCGGACGCAGGTGCTGCGTTGCGCCGACAAGGCGGGCCGGCCGGTACGTGAATGGCAAGTGCTAACACCGGC
GGCGGACTTCCCTTCCCATGACGGGCTGCCCCCACTCAAGACCCTGATTCTGCGGCTTTGA

Upstream 100 bases:

>100_bases
CGACCAGCTGCACCAGCAGGGCACCGATTGCTACCGCCTGTTCCATGGCAGCCAGGAAGGTGCTGCCGGCCTGACGGTGG
ATCGCTACGGGCCGCAGCTG

Downstream 100 bases:

>100_bases
GCGCATTGCCGGTATCGCTCGATTCGGGGAACCGCCTGGCCATGCCATACTCCAAGGCATCCATCCGCCAGGAATACTTC
CGGTCATGCCCAATGGAATG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVQSFHQTLAADALLRIHELAMDALELPLQLVYNDRSQGNSRIDRSHAAYQPDAAALADQVGHEWGLAYRVRGRHDGQDP
LLFLDLRNARGWVKQHSAGKSVLNLFSYTCGVGLCAAAGGARDVWNLDFAERNLAVGRENAALNPGLAPMRFVRSDYFPA
IRQLAGLPVASRRGRALPDYPRLEQRQFDLVLLDPPAWARSAFGTVDLLRDYQSLLKPALLTTAEGGVLICCNNLAKVPM
DEWRTQVLRCADKAGRPVREWQVLTPAADFPSHDGLPPLKTLILRL

Sequences:

>Translated_286_residues
MVQSFHQTLAADALLRIHELAMDALELPLQLVYNDRSQGNSRIDRSHAAYQPDAAALADQVGHEWGLAYRVRGRHDGQDP
LLFLDLRNARGWVKQHSAGKSVLNLFSYTCGVGLCAAAGGARDVWNLDFAERNLAVGRENAALNPGLAPMRFVRSDYFPA
IRQLAGLPVASRRGRALPDYPRLEQRQFDLVLLDPPAWARSAFGTVDLLRDYQSLLKPALLTTAEGGVLICCNNLAKVPM
DEWRTQVLRCADKAGRPVREWQVLTPAADFPSHDGLPPLKTLILRL
>Mature_286_residues
MVQSFHQTLAADALLRIHELAMDALELPLQLVYNDRSQGNSRIDRSHAAYQPDAAALADQVGHEWGLAYRVRGRHDGQDP
LLFLDLRNARGWVKQHSAGKSVLNLFSYTCGVGLCAAAGGARDVWNLDFAERNLAVGRENAALNPGLAPMRFVRSDYFPA
IRQLAGLPVASRRGRALPDYPRLEQRQFDLVLLDPPAWARSAFGTVDLLRDYQSLLKPALLTTAEGGVLICCNNLAKVPM
DEWRTQVLRCADKAGRPVREWQVLTPAADFPSHDGLPPLKTLILRL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=210, Percent_Identity=28.0952380952381, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787181, Length=170, Percent_Identity=31.7647058823529, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 31692; Mature: 31692

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSFHQTLAADALLRIHELAMDALELPLQLVYNDRSQGNSRIDRSHAAYQPDAAALADQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHCCCCCHHHHHHHH
VGHEWGLAYRVRGRHDGQDPLLFLDLRNARGWVKQHSAGKSVLNLFSYTCGVGLCAAAGG
HCHHHCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARDVWNLDFAERNLAVGRENAALNPGLAPMRFVRSDYFPAIRQLAGLPVASRRGRALPDY
CCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PRLEQRQFDLVLLDPPAWARSAFGTVDLLRDYQSLLKPALLTTAEGGVLICCNNLAKVPM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCH
DEWRTQVLRCADKAGRPVREWQVLTPAADFPSHDGLPPLKTLILRL
HHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MVQSFHQTLAADALLRIHELAMDALELPLQLVYNDRSQGNSRIDRSHAAYQPDAAALADQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHCCCCCHHHHHHHH
VGHEWGLAYRVRGRHDGQDPLLFLDLRNARGWVKQHSAGKSVLNLFSYTCGVGLCAAAGG
HCHHHCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARDVWNLDFAERNLAVGRENAALNPGLAPMRFVRSDYFPAIRQLAGLPVASRRGRALPDY
CCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PRLEQRQFDLVLLDPPAWARSAFGTVDLLRDYQSLLKPALLTTAEGGVLICCNNLAKVPM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCH
DEWRTQVLRCADKAGRPVREWQVLTPAADFPSHDGLPPLKTLILRL
HHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA