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The map label for this gene is pcnB [H]

Identifier: 146283614

GI number: 146283614

Start: 3566014

End: 3567414

Strand: Direct

Name: pcnB [H]

Synonym: PST_3294

Alternate gene names: 146283614

Gene position: 3566014-3567414 (Clockwise)

Preceding gene: 146283613

Following gene: 146283615

Centisome position: 78.08

GC content: 67.09

Gene sequence:

>1401_bases
TTGCCCCTCATGCTGAAAAAGCTGTTCCAGTCTTTTCGTCTTCCGCTGCGCCGCATCCCGCATCCCCGTCGCACACCTGA
AATTCTGTCCAGCCGGCAGCATTCCCTGCATCGCAACGAGATCAGCCGTCATGCGGTGAGCGTGGTCGAGCGCCTGCAGC
AGGCCGGCTACGAGGCCTATGTCGTGGGTGGCTGTGTGCGCGATCTGTTGCTGCAGCTCAATCCCAAGGATTACGACGTC
GCCACCAGTGCGACCCCGGAACAGGTCCGCGCCGAGTTTCGCAACGCACGCGTGATCGGCCGGCGCTTCAAGCTCGTCCA
CGTGCACTTCGGCCGCGAGATCATCGAAGTCGCCACCTTCCGCGCCAACCACCCGGAAGAAGACGACGAGGATGCCAGCC
AGTTGGCCTCGCGCAACGAGAGCGGGCGCATCCTGCGCGACAACGTCTATGGCACGCTCGAGGACGACGCCCAGCGCCGG
GACTTCACCATCAATGCGCTGTACTACGACCCGACCAGCGAGCGCATCCTCGACCACACCCGCGGCGTGCACGACATCCG
CAATCGCCTGATTCGCCTGATCGGCGATCCCGAGCAGCGCTACCAGGAAGACCCGGTACGCATGCTCCGCGCGGTGCGCT
TCGCCGCCAAGCTCGACTTCGAAATCGAACGCCACAGTGCCGAGCCCATCGCCGACCTGGCCGAGCTGCTCGACGGCATC
CCCTCGGCCCGCCTGTTCGACGAGACGCTCAAGCTGTTCCTCGGCGGCAAGGCCGAGCGAACCTTCGAGCTGCTGCTCGA
ATACGACCTGTTTGCGCCGCTGTTCCCCGCCAGCGCCGCCGCGCTGGAGCGCAACCCCGAGTATGCCGGCACGCTGATCC
GCAACGCGCTGGCCAACACCGACCTGCGCATCCAGCAGGGCAAGCCGGTGACGCCCGCGTTCCTTTTCGCCGCCCTGCTC
TGGCCTGCCCTGCCGGCCCGCGTACTGGAGCTGCAGGACCGCGGCATGCCGCCGATTCCGGCGATGCAGGAAGCCGCCCA
CGACATCATCTGGGAGCAGTGCCAGCGCACCGCGATTCCCAAGCGCTTCACCATCCCCATGCGCGAGATCTGGGACATGC
AGGAACGCCTGCCGCGCCGGCAGGGCCGCCGTGCCGACCAGCTTCTGGAGAATCCGCGCTTCCGCGCTGGCTACGACTTT
CTCCTGCTGCGCGAAAGCGCGGGCGAGAAGACCGGCGGCCTTGGCGACTGGTGGACCGACTATCAGGAAGCCAACGACAG
CGAGCGGCGCAACATGATCCGCGGCCTCAGCGGCAAGGATGACGCCGGCGCGGCCAAGAAACGCCGCCGTGGCGGTCGCC
GTCGCCGCACGCCGGGCGATGCCGCCAACCCGGCCGACTGA

Upstream 100 bases:

>100_bases
ACACTACCCTGCGCTTCGTTCACCATCCGGCAGTCCCGGTGCTAGAATCCGCGCAGGTTTCGCGGCTTTCGCGACGCCGG
CCCGTCATTCCGCCACACAG

Downstream 100 bases:

>100_bases
GATGGAGCGCGTCTACATCGGCCTGGGCAGCAACCTGGCCGATCCCCAGCAGCAGCTGCGTGGTGCCCTGCAGGCTCTGG
CCGAGCTGCCAGGTAGCAGG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPLMLKKLFQSFRLPLRRIPHPRRTPEILSSRQHSLHRNEISRHAVSVVERLQQAGYEAYVVGGCVRDLLLQLNPKDYDV
ATSATPEQVRAEFRNARVIGRRFKLVHVHFGREIIEVATFRANHPEEDDEDASQLASRNESGRILRDNVYGTLEDDAQRR
DFTINALYYDPTSERILDHTRGVHDIRNRLIRLIGDPEQRYQEDPVRMLRAVRFAAKLDFEIERHSAEPIADLAELLDGI
PSARLFDETLKLFLGGKAERTFELLLEYDLFAPLFPASAAALERNPEYAGTLIRNALANTDLRIQQGKPVTPAFLFAALL
WPALPARVLELQDRGMPPIPAMQEAAHDIIWEQCQRTAIPKRFTIPMREIWDMQERLPRRQGRRADQLLENPRFRAGYDF
LLLRESAGEKTGGLGDWWTDYQEANDSERRNMIRGLSGKDDAGAAKKRRRGGRRRRTPGDAANPAD

Sequences:

>Translated_466_residues
MPLMLKKLFQSFRLPLRRIPHPRRTPEILSSRQHSLHRNEISRHAVSVVERLQQAGYEAYVVGGCVRDLLLQLNPKDYDV
ATSATPEQVRAEFRNARVIGRRFKLVHVHFGREIIEVATFRANHPEEDDEDASQLASRNESGRILRDNVYGTLEDDAQRR
DFTINALYYDPTSERILDHTRGVHDIRNRLIRLIGDPEQRYQEDPVRMLRAVRFAAKLDFEIERHSAEPIADLAELLDGI
PSARLFDETLKLFLGGKAERTFELLLEYDLFAPLFPASAAALERNPEYAGTLIRNALANTDLRIQQGKPVTPAFLFAALL
WPALPARVLELQDRGMPPIPAMQEAAHDIIWEQCQRTAIPKRFTIPMREIWDMQERLPRRQGRRADQLLENPRFRAGYDF
LLLRESAGEKTGGLGDWWTDYQEANDSERRNMIRGLSGKDDAGAAKKRRRGGRRRRTPGDAANPAD
>Mature_465_residues
PLMLKKLFQSFRLPLRRIPHPRRTPEILSSRQHSLHRNEISRHAVSVVERLQQAGYEAYVVGGCVRDLLLQLNPKDYDVA
TSATPEQVRAEFRNARVIGRRFKLVHVHFGREIIEVATFRANHPEEDDEDASQLASRNESGRILRDNVYGTLEDDAQRRD
FTINALYYDPTSERILDHTRGVHDIRNRLIRLIGDPEQRYQEDPVRMLRAVRFAAKLDFEIERHSAEPIADLAELLDGIP
SARLFDETLKLFLGGKAERTFELLLEYDLFAPLFPASAAALERNPEYAGTLIRNALANTDLRIQQGKPVTPAFLFAALLW
PALPARVLELQDRGMPPIPAMQEAAHDIIWEQCQRTAIPKRFTIPMREIWDMQERLPRRQGRRADQLLENPRFRAGYDFL
LLRESAGEKTGGLGDWWTDYQEANDSERRNMIRGLSGKDDAGAAKKRRRGGRRRRTPGDAANPAD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=246, Percent_Identity=31.3008130081301, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081691, Length=413, Percent_Identity=48.6682808716707, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=32.0175438596491, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=214, Percent_Identity=30.8411214953271, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360112, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357337, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53517; Mature: 53385

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLMLKKLFQSFRLPLRRIPHPRRTPEILSSRQHSLHRNEISRHAVSVVERLQQAGYEAY
CCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVGGCVRDLLLQLNPKDYDVATSATPEQVRAEFRNARVIGRRFKLVHVHFGREIIEVATF
EHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHH
RANHPEEDDEDASQLASRNESGRILRDNVYGTLEDDAQRRDFTINALYYDPTSERILDHT
CCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
RGVHDIRNRLIRLIGDPEQRYQEDPVRMLRAVRFAAKLDFEIERHSAEPIADLAELLDGI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCCCHHHHHHHHHCC
PSARLFDETLKLFLGGKAERTFELLLEYDLFAPLFPASAAALERNPEYAGTLIRNALANT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCC
DLRIQQGKPVTPAFLFAALLWPALPARVLELQDRGMPPIPAMQEAAHDIIWEQCQRTAIP
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
KRFTIPMREIWDMQERLPRRQGRRADQLLENPRFRAGYDFLLLRESAGEKTGGLGDWWTD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
YQEANDSERRNMIRGLSGKDDAGAAKKRRRGGRRRRTPGDAANPAD
HHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLMLKKLFQSFRLPLRRIPHPRRTPEILSSRQHSLHRNEISRHAVSVVERLQQAGYEAY
CHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVGGCVRDLLLQLNPKDYDVATSATPEQVRAEFRNARVIGRRFKLVHVHFGREIIEVATF
EHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHH
RANHPEEDDEDASQLASRNESGRILRDNVYGTLEDDAQRRDFTINALYYDPTSERILDHT
CCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
RGVHDIRNRLIRLIGDPEQRYQEDPVRMLRAVRFAAKLDFEIERHSAEPIADLAELLDGI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCCCHHHHHHHHHCC
PSARLFDETLKLFLGGKAERTFELLLEYDLFAPLFPASAAALERNPEYAGTLIRNALANT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCC
DLRIQQGKPVTPAFLFAALLWPALPARVLELQDRGMPPIPAMQEAAHDIIWEQCQRTAIP
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
KRFTIPMREIWDMQERLPRRQGRRADQLLENPRFRAGYDFLLLRESAGEKTGGLGDWWTD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
YQEANDSERRNMIRGLSGKDDAGAAKKRRRGGRRRRTPGDAANPAD
HHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]