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The map label for this gene is dksA [H]

Identifier: 146283609

GI number: 146283609

Start: 3559154

End: 3559600

Strand: Direct

Name: dksA [H]

Synonym: PST_3289

Alternate gene names: 146283609

Gene position: 3559154-3559600 (Clockwise)

Preceding gene: 146283604

Following gene: 146283610

Centisome position: 77.92

GC content: 59.73

Gene sequence:

>447_bases
ATGCCCATTACCAAAGAAACACCGTCGAGCAAGCAACTGATTCGCGCCTTCGAGCCCTACAAGGAAAGCCAGGGCGAGGA
ATACATGAGCGCCAAGATGCGCGCCCACTTCACCGGCATCCTGAACAAGTGGAAGCAGGAGCTGATGGAAGAGGTCGATC
GCACCGTGCACCACATGCAGGACGAGGCTGCCAACTTCCCGGATCCGGCCGACCGCGCCAGTCAGGAAGAAGAGTTCAGC
CTGGAACTGCGCGCCCGCGACCGCGAGCGCAAGCTGATCAAGAAGATCGACGAGACGCTGCAGCTGATCGAAGACAACGA
ATACGGCTGGTGCGATTCCTGCGGTGTCGAAATCGGCATCCGTCGCCTGGAAGCGCGCCCCACCGCCACCCTCTGCATCG
ATTGCAAGACGCTGGCGGAGATCAAGGAAAAGCAGATCGGTTCCTGA

Upstream 100 bases:

>100_bases
GCCGATCTGGTAAGTTCGCCCGCTTGCAGCCGCAGGGCCGGCACGGGCCGGCAAGGGACAAATCATCCTGCGCAATGGAC
ATAGCGAGAGGCGTTCATCC

Downstream 100 bases:

>100_bases
TCCCGAACGGGGCGCTACGGCGCCCCGCTTCATTTCCAGGCCCTGCACCGTTTCATCCGCCTCGCCATCACCGATCGCGC
CGGCCCGCCATGTCCTCCTC

Product: suppressor protein DksA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MPITKETPSSKQLIRAFEPYKESQGEEYMSAKMRAHFTGILNKWKQELMEEVDRTVHHMQDEAANFPDPADRASQEEEFS
LELRARDRERKLIKKIDETLQLIEDNEYGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIKEKQIGS

Sequences:

>Translated_148_residues
MPITKETPSSKQLIRAFEPYKESQGEEYMSAKMRAHFTGILNKWKQELMEEVDRTVHHMQDEAANFPDPADRASQEEEFS
LELRARDRERKLIKKIDETLQLIEDNEYGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIKEKQIGS
>Mature_147_residues
PITKETPSSKQLIRAFEPYKESQGEEYMSAKMRAHFTGILNKWKQELMEEVDRTVHHMQDEAANFPDPADRASQEEEFSL
ELRARDRERKLIKKIDETLQLIEDNEYGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIKEKQIGS

Specific function: Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed [H]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=135, Percent_Identity=71.1111111111111, Blast_Score=204, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR020460
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 17191; Mature: 17059

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITKETPSSKQLIRAFEPYKESQGEEYMSAKMRAHFTGILNKWKQELMEEVDRTVHHMQ
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAANFPDPADRASQEEEFSLELRARDRERKLIKKIDETLQLIEDNEYGWCDSCGVEIGI
HHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RRLEARPTATLCIDCKTLAEIKEKQIGS
HHHCCCCCEEEEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
PITKETPSSKQLIRAFEPYKESQGEEYMSAKMRAHFTGILNKWKQELMEEVDRTVHHMQ
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAANFPDPADRASQEEEFSLELRARDRERKLIKKIDETLQLIEDNEYGWCDSCGVEIGI
HHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RRLEARPTATLCIDCKTLAEIKEKQIGS
HHHCCCCCEEEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]