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The map label for this gene is hmpA [H]

Identifier: 146283448

GI number: 146283448

Start: 3360059

End: 3361366

Strand: Direct

Name: hmpA [H]

Synonym: PST_3121

Alternate gene names: 146283448

Gene position: 3360059-3361366 (Clockwise)

Preceding gene: 146283447

Following gene: 146283449

Centisome position: 73.57

GC content: 64.68

Gene sequence:

>1308_bases
GTGAATTTCACCAGCAACGCCCCGAAAACCTTGATTCAAGTCAATTTCAGAGCTGGAACGATTGTTGAAAGCCCTCTGCA
TGACGTTCGCCACGGCGACCGTAGGTTTTTCCCACCAGAGGAATATTCGATGCTTTGCGCTGAACAGATCGCCCTTATCA
AAGCCACCGTGCCGTTGCTGGAAAGCGGTGGCGAAGCCCTGACCAACCATTTCTACAAGCTGCTGCTGAGCGAGCATCCC
GAGGTTCGCCCGCTGTTCAACCAGGCACACCAGGCCAGCGGCGAGCAACCGCGCGCCCTGGCCAACGGCGTGCTGATGTA
TGCCCGGCATATCGACCGGCTGGACGCTCTCGGCCCGCTGGTGGCACAGATCATCAACAAGCACGTCGCCCTGCAGGTGC
TACCCGAGCATTACCCACTGGTCGGCAATTGCTTGCTGCGCGCCATCCGCGAGGTGCTTGGCGAGGCCATCGCCACCGAT
GCGGTGATCGACGCCTGGGCGGCGGCCTATCAGCAGCTCGCCGACCTGCTCATCGGCCAGGAAGAACGCCTCTACCAGGC
CAAGGCCGAAGCGCCGGGAGGCTGGCGTGGCGCGCGCCCGTTCCGCATTGCGCGCAAGGTCAAGGAGAGCGAGGAGATCA
CCTCGTTTCACCTGCAAGCGGAGGATGGCGGGCCGCTGATGGAGTTCCTGCCCGGCCAGTACATCGGTCTGAGGCTGGAA
ATCAATGGCAAGGAAGAGCGCCGCAACTACTCGTTGTCCGCTGCCGGCAACGGTCGCGAGTATCGCATCAGCGTCAAGCG
CGAGCCTGGCGGCGTGGTCTCCAATGCGCTGCACGACATGCCGGAGGGCGCGATCGTCGAGCTGTTCGCGCCGGCAGGCG
AGTTCACCCTTGCGCCCGGGAACAAGCCGTTGGTGCTGATCAGCGGCGGCGTGGGGATCACCCCGACCCTGGCGATGCTG
GAGCAGGCGCTGGCGACGTCCCGGCCGGTGCACTTCATCCACTGCGCGCGCAATGCCGGGGTGCACGCCTTCCGCCGCGC
CGTGGACGCACTCGCCGAGCGCCACACGCAGCTCCAGCGCTTCTACTGCTACGAGGAGCACGACGGCTCGGCTGACGCAC
CGGACGCCATCGGCCGGCTGACCGAACAGCAGTTGGCGGACTGGCTACCGCAGAGCCGCGATGTCGACGCCTACTTCCTC
GGCCCGAAACCGTTCATGGCAGCCGTGCGCCGGCAACTCAAGGCCATTGGCGTGCCGGAGCAGCAAACGCGCTACGAGTT
CTTCGGTCCCGCATCAGCCCTCGATTGA

Upstream 100 bases:

>100_bases
GGGGCTCTCCCATAGGAAAGCCGGACGGCGACGAAGGTTCGGCACGCAAGCGGAGTGTACATTCCACCCTCATGGGTTCG
GATGACCCGTGCAAATCAGG

Downstream 100 bases:

>100_bases
CCCATTCGAGGCTCGCGCCACGCGAGCCTCCTCCAGAGGAGATTCAGTCATGCCGTTCCAGCCCGGTTGCTTCCGAAACC
TGTGGTTCATGGCCAACAGC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNFTSNAPKTLIQVNFRAGTIVESPLHDVRHGDRRFFPPEEYSMLCAEQIALIKATVPLLESGGEALTNHFYKLLLSEHP
EVRPLFNQAHQASGEQPRALANGVLMYARHIDRLDALGPLVAQIINKHVALQVLPEHYPLVGNCLLRAIREVLGEAIATD
AVIDAWAAAYQQLADLLIGQEERLYQAKAEAPGGWRGARPFRIARKVKESEEITSFHLQAEDGGPLMEFLPGQYIGLRLE
INGKEERRNYSLSAAGNGREYRISVKREPGGVVSNALHDMPEGAIVELFAPAGEFTLAPGNKPLVLISGGVGITPTLAML
EQALATSRPVHFIHCARNAGVHAFRRAVDALAERHTQLQRFYCYEEHDGSADAPDAIGRLTEQQLADWLPQSRDVDAYFL
GPKPFMAAVRRQLKAIGVPEQQTRYEFFGPASALD

Sequences:

>Translated_435_residues
MNFTSNAPKTLIQVNFRAGTIVESPLHDVRHGDRRFFPPEEYSMLCAEQIALIKATVPLLESGGEALTNHFYKLLLSEHP
EVRPLFNQAHQASGEQPRALANGVLMYARHIDRLDALGPLVAQIINKHVALQVLPEHYPLVGNCLLRAIREVLGEAIATD
AVIDAWAAAYQQLADLLIGQEERLYQAKAEAPGGWRGARPFRIARKVKESEEITSFHLQAEDGGPLMEFLPGQYIGLRLE
INGKEERRNYSLSAAGNGREYRISVKREPGGVVSNALHDMPEGAIVELFAPAGEFTLAPGNKPLVLISGGVGITPTLAML
EQALATSRPVHFIHCARNAGVHAFRRAVDALAERHTQLQRFYCYEEHDGSADAPDAIGRLTEQQLADWLPQSRDVDAYFL
GPKPFMAAVRRQLKAIGVPEQQTRYEFFGPASALD
>Mature_435_residues
MNFTSNAPKTLIQVNFRAGTIVESPLHDVRHGDRRFFPPEEYSMLCAEQIALIKATVPLLESGGEALTNHFYKLLLSEHP
EVRPLFNQAHQASGEQPRALANGVLMYARHIDRLDALGPLVAQIINKHVALQVLPEHYPLVGNCLLRAIREVLGEAIATD
AVIDAWAAAYQQLADLLIGQEERLYQAKAEAPGGWRGARPFRIARKVKESEEITSFHLQAEDGGPLMEFLPGQYIGLRLE
INGKEERRNYSLSAAGNGREYRISVKREPGGVVSNALHDMPEGAIVELFAPAGEFTLAPGNKPLVLISGGVGITPTLAML
EQALATSRPVHFIHCARNAGVHAFRRAVDALAERHTQLQRFYCYEEHDGSADAPDAIGRLTEQQLADWLPQSRDVDAYFL
GPKPFMAAVRRQLKAIGVPEQQTRYEFFGPASALD

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=402, Percent_Identity=39.8009950248756, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1787658, Length=217, Percent_Identity=29.9539170506912, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788104, Length=225, Percent_Identity=27.5555555555556, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=409, Percent_Identity=36.6748166259169, Blast_Score=217, Evalue=3e-57,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 48027; Mature: 48027

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTSNAPKTLIQVNFRAGTIVESPLHDVRHGDRRFFPPEEYSMLCAEQIALIKATVPLL
CCCCCCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESGGEALTNHFYKLLLSEHPEVRPLFNQAHQASGEQPRALANGVLMYARHIDRLDALGPL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAQIINKHVALQVLPEHYPLVGNCLLRAIREVLGEAIATDAVIDAWAAAYQQLADLLIGQ
HHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EERLYQAKAEAPGGWRGARPFRIARKVKESEEITSFHLQAEDGGPLMEFLPGQYIGLRLE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHCCCCEEEEEEE
INGKEERRNYSLSAAGNGREYRISVKREPGGVVSNALHDMPEGAIVELFAPAGEFTLAPG
ECCCHHHCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCEEECCC
NKPLVLISGGVGITPTLAMLEQALATSRPVHFIHCARNAGVHAFRRAVDALAERHTQLQR
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
FYCYEEHDGSADAPDAIGRLTEQQLADWLPQSRDVDAYFLGPKPFMAAVRRQLKAIGVPE
HHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
QQTRYEFFGPASALD
HHCCCEECCCHHCCC
>Mature Secondary Structure
MNFTSNAPKTLIQVNFRAGTIVESPLHDVRHGDRRFFPPEEYSMLCAEQIALIKATVPLL
CCCCCCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESGGEALTNHFYKLLLSEHPEVRPLFNQAHQASGEQPRALANGVLMYARHIDRLDALGPL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAQIINKHVALQVLPEHYPLVGNCLLRAIREVLGEAIATDAVIDAWAAAYQQLADLLIGQ
HHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EERLYQAKAEAPGGWRGARPFRIARKVKESEEITSFHLQAEDGGPLMEFLPGQYIGLRLE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHCCCCEEEEEEE
INGKEERRNYSLSAAGNGREYRISVKREPGGVVSNALHDMPEGAIVELFAPAGEFTLAPG
ECCCHHHCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCEEECCC
NKPLVLISGGVGITPTLAMLEQALATSRPVHFIHCARNAGVHAFRRAVDALAERHTQLQR
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
FYCYEEHDGSADAPDAIGRLTEQQLADWLPQSRDVDAYFLGPKPFMAAVRRQLKAIGVPE
HHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
QQTRYEFFGPASALD
HHCCCEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]