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The map label for this gene is pykF [H]

Identifier: 146283445

GI number: 146283445

Start: 3357326

End: 3358753

Strand: Direct

Name: pykF [H]

Synonym: PST_3118

Alternate gene names: 146283445

Gene position: 3357326-3358753 (Clockwise)

Preceding gene: 146283444

Following gene: 146283446

Centisome position: 73.51

GC content: 69.4

Gene sequence:

>1428_bases
ATGACACACGACAAGAAGGTCAAGATTCTCGCTACCCTCGGCCCGGCCACCCGCAGCGTCGACGACGTGCGCGCACTGGT
CGAGGCCGGCGTGAACCTGTTCCGCCTGAACTTCAGCCACGGCGAGCACAGCGACCACGCCGAGCGCTTCGCCTGGATTC
GCCAGGTCGAGCAGGAGCTGAACCAGCCGATCGGCATCCTCATGGACCTGCAGGGGCCCAAGCTCAGGGTCGGCCGTTTC
GCCGCCGGCAAGGTCCGCCTTGAGCGTGACCAGGCCTTTCGTCTGGACCTGGACCCGACACCGGGCGACAGCCGGCGCGT
GAACCTGCCGCACCCGGAGATCATCGCCGCGCTGGAACCGGGCATGCAGCTGCTGATCGACGATGGTCGCCTGCGCCTGA
CGGTCGTCGAGCGCCATGCCGACGCCATCGAGACCCGCGTGGGGGCCGGCGGCGAGCTGTCCGACCGCAAGGGCGTGAAC
GTGCCGGAGGCGGTGCTGGAGCTTTCGCCGCTGACGGCGAAGGACCGCCAGGACCTGACCTTCGGCCTCGAGCTGGGCGT
GGACTGGGTGGCGCTGTCGTTCGTGCAACGCCCGCAGGACATCCAGGAGGCGCGCAAGCTGATCGGCGAGCGGGCCTTCC
TCATGGCCAAGATCGAAAAGCCTTCCGCGGTGCAGCACCTGCGCGAGATCGCCAGGCTGTCGGACGCGATCATGGTGGCG
CGTGGCGACCTGGGCGTGGAGGTACCGCCGGAAAACGTGCCGCGCATCCAGAAGAACATCGTGCGCATCTGCCGCCAGCT
CGGCCGCCCGGTGGTGGTTGCGACGCAGATGCTCGAATCCATGCGCTTCGCCCCGGCCCCGACCCGTGCCGAAGTCACCG
ACGTGGCCAACGCCGTGGCCAACGCCGTGGCCGAGGGTGCGGACGCGGTGATGCTGTCGGCGGAAACCGCCTCCGGCGAC
TACCCGCTGGAAGCGGTGAGCATGATGAGCAAGATCATCCGCCAGGTGGAAAGCGGCCCGGACTTCCAGGCGCAGCTCGA
CGTGCACCGGCCCAACGCCGAGGCGACCCTGCCGGATGCCATCAGCTGCGCGATCCGCCGCATCAGCGGCATCCTGCCGG
TGGCGGTGCTGGTCAACTACACCGAATCCGGCCGTTCCAGCCTGCGCGCCTCGCGCGAGCGGCCGGCCACGCCGATCCTC
AGCCTGACGCCCAGCACCGCCACCGCACGCAAGCTGACGGTGGCCTGGGGCGTGTACTCGGTGGTGGATGCGCCGATGCG
CGACATGGAGCAGGTCAGCGGCCACGCCGTCGAGCTGGCTCGGGCGCAGGGCATGGCCGAGAGCGGCGATACGGTGCTGA
TCACCGCCGGTGTGCCACTGGGCCAGCCGGGTACCACCAACTCGCTGCGCATCGAAGTCCTGCCCTGA

Upstream 100 bases:

>100_bases
TCAACGACTTTCGCGCCATCCTGATCCTGCCGTCGTCGGATTGACGGCCTCCACCTCGTTCAGGCGCGCATCAGAACAAC
AACTGCCCCAGAGCACCGCC

Downstream 100 bases:

>100_bases
ATGGAGCCGGGCGCAAGCCCGGCCCACCTCCCCACGACAGCGGTCGTCGACCCCTTGCGGGTCGGCACCCTTGCGCTCGC
CTGGATTTCGCCGATGCCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTHDKKVKILATLGPATRSVDDVRALVEAGVNLFRLNFSHGEHSDHAERFAWIRQVEQELNQPIGILMDLQGPKLRVGRF
AAGKVRLERDQAFRLDLDPTPGDSRRVNLPHPEIIAALEPGMQLLIDDGRLRLTVVERHADAIETRVGAGGELSDRKGVN
VPEAVLELSPLTAKDRQDLTFGLELGVDWVALSFVQRPQDIQEARKLIGERAFLMAKIEKPSAVQHLREIARLSDAIMVA
RGDLGVEVPPENVPRIQKNIVRICRQLGRPVVVATQMLESMRFAPAPTRAEVTDVANAVANAVAEGADAVMLSAETASGD
YPLEAVSMMSKIIRQVESGPDFQAQLDVHRPNAEATLPDAISCAIRRISGILPVAVLVNYTESGRSSLRASRERPATPIL
SLTPSTATARKLTVAWGVYSVVDAPMRDMEQVSGHAVELARAQGMAESGDTVLITAGVPLGQPGTTNSLRIEVLP

Sequences:

>Translated_475_residues
MTHDKKVKILATLGPATRSVDDVRALVEAGVNLFRLNFSHGEHSDHAERFAWIRQVEQELNQPIGILMDLQGPKLRVGRF
AAGKVRLERDQAFRLDLDPTPGDSRRVNLPHPEIIAALEPGMQLLIDDGRLRLTVVERHADAIETRVGAGGELSDRKGVN
VPEAVLELSPLTAKDRQDLTFGLELGVDWVALSFVQRPQDIQEARKLIGERAFLMAKIEKPSAVQHLREIARLSDAIMVA
RGDLGVEVPPENVPRIQKNIVRICRQLGRPVVVATQMLESMRFAPAPTRAEVTDVANAVANAVAEGADAVMLSAETASGD
YPLEAVSMMSKIIRQVESGPDFQAQLDVHRPNAEATLPDAISCAIRRISGILPVAVLVNYTESGRSSLRASRERPATPIL
SLTPSTATARKLTVAWGVYSVVDAPMRDMEQVSGHAVELARAQGMAESGDTVLITAGVPLGQPGTTNSLRIEVLP
>Mature_474_residues
THDKKVKILATLGPATRSVDDVRALVEAGVNLFRLNFSHGEHSDHAERFAWIRQVEQELNQPIGILMDLQGPKLRVGRFA
AGKVRLERDQAFRLDLDPTPGDSRRVNLPHPEIIAALEPGMQLLIDDGRLRLTVVERHADAIETRVGAGGELSDRKGVNV
PEAVLELSPLTAKDRQDLTFGLELGVDWVALSFVQRPQDIQEARKLIGERAFLMAKIEKPSAVQHLREIARLSDAIMVAR
GDLGVEVPPENVPRIQKNIVRICRQLGRPVVVATQMLESMRFAPAPTRAEVTDVANAVANAVAEGADAVMLSAETASGDY
PLEAVSMMSKIIRQVESGPDFQAQLDVHRPNAEATLPDAISCAIRRISGILPVAVLVNYTESGRSSLRASRERPATPILS
LTPSTATARKLTVAWGVYSVVDAPMRDMEQVSGHAVELARAQGMAESGDTVLITAGVPLGQPGTTNSLRIEVLP

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=37.6782077393075, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=37.6782077393075, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=37.2983870967742, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.2903225806452, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.2903225806452, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=37.1024734982332, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=37.1024734982332, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI310128736, Length=232, Percent_Identity=34.4827586206897, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128734, Length=232, Percent_Identity=34.4827586206897, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=39.3305439330544, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=35.9243697478992, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=35.523613963039, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=37.1663244353183, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=37.1663244353183, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=37.1663244353183, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=37.1663244353183, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=33.8114754098361, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=31.5573770491803, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=34.0122199592668, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=34.0122199592668, Blast_Score=277, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24581235, Length=494, Percent_Identity=31.1740890688259, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=34.6987951807229, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24646914, Length=323, Percent_Identity=35.6037151702786, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51571; Mature: 51440

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDKKVKILATLGPATRSVDDVRALVEAGVNLFRLNFSHGEHSDHAERFAWIRQVEQEL
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
NQPIGILMDLQGPKLRVGRFAAGKVRLERDQAFRLDLDPTPGDSRRVNLPHPEIIAALEP
CCCCEEEEECCCCCEEECEEECCEEEEECCCEEEEECCCCCCCCCEECCCCHHHHHHHCC
GMQLLIDDGRLRLTVVERHADAIETRVGAGGELSDRKGVNVPEAVLELSPLTAKDRQDLT
CCEEEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCE
FGLELGVDWVALSFVQRPQDIQEARKLIGERAFLMAKIEKPSAVQHLREIARLSDAIMVA
EEHHHCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEE
RGDLGVEVPPENVPRIQKNIVRICRQLGRPVVVATQMLESMRFAPAPTRAEVTDVANAVA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NAVAEGADAVMLSAETASGDYPLEAVSMMSKIIRQVESGPDFQAQLDVHRPNAEATLPDA
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHH
ISCAIRRISGILPVAVLVNYTESGRSSLRASRERPATPILSLTPSTATARKLTVAWGVYS
HHHHHHHHHCHHEEHHHEECCHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEEHHHHHH
VVDAPMRDMEQVSGHAVELARAQGMAESGDTVLITAGVPLGQPGTTNSLRIEVLP
HHHCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
THDKKVKILATLGPATRSVDDVRALVEAGVNLFRLNFSHGEHSDHAERFAWIRQVEQEL
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
NQPIGILMDLQGPKLRVGRFAAGKVRLERDQAFRLDLDPTPGDSRRVNLPHPEIIAALEP
CCCCEEEEECCCCCEEECEEECCEEEEECCCEEEEECCCCCCCCCEECCCCHHHHHHHCC
GMQLLIDDGRLRLTVVERHADAIETRVGAGGELSDRKGVNVPEAVLELSPLTAKDRQDLT
CCEEEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCE
FGLELGVDWVALSFVQRPQDIQEARKLIGERAFLMAKIEKPSAVQHLREIARLSDAIMVA
EEHHHCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEE
RGDLGVEVPPENVPRIQKNIVRICRQLGRPVVVATQMLESMRFAPAPTRAEVTDVANAVA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NAVAEGADAVMLSAETASGDYPLEAVSMMSKIIRQVESGPDFQAQLDVHRPNAEATLPDA
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHH
ISCAIRRISGILPVAVLVNYTESGRSSLRASRERPATPILSLTPSTATARKLTVAWGVYS
HHHHHHHHHCHHEEHHHEECCHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEEHHHHHH
VVDAPMRDMEQVSGHAVELARAQGMAESGDTVLITAGVPLGQPGTTNSLRIEVLP
HHHCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA