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The map label for this gene is gcl [H]

Identifier: 146283441

GI number: 146283441

Start: 3351969

End: 3353750

Strand: Direct

Name: gcl [H]

Synonym: PST_3114

Alternate gene names: 146283441

Gene position: 3351969-3353750 (Clockwise)

Preceding gene: 146283439

Following gene: 146283442

Centisome position: 73.39

GC content: 67.0

Gene sequence:

>1782_bases
ATGGCCCGAATGAGAGCAATCGATGCCGCCGTCGCGGTGTTGCGCAAGGAAGGCATCGACACCGCCTTCGGCATCCCCGG
AGCCGCGATCAATCCGCTGTATTCCGCCCTGCGGGCCGATGGCGGCATCCGTCATATCCTCGCCCGCCACGTCGAGGGTG
CCTCGCACATGGCCGAGGGCTATACCCGTGCCAAGCCAGGCAACATCGGCGTGTGCATCGGCACCTCCGGCCCGGCCGGC
ACCGACATGATCACCGGCCTGTACTCGGCTTCCGCCGACTCCATTCCGATCCTCTGCATCACCGGGCAGGCACCGCGTGC
CCGCCTGCACAAGGAAGATTTCCAGGCCGTGGACATCGAGTCCATCGCCAAGCCGGTCACCAAGTGGGCGGTCACCGTAC
TCGAGCCGGCACTGGTCCCGCGCGTGTTCCAGCAGGCCTTCCACGTGATGCGCTCCGGCCGCCCCGGCCCGGTGCTGATC
GACCTGCCGTTCGACGTGCAGATGGCCGAGATCGAGTTCGACGTCGACACCTACGAGCCGCTGCCGGTCTATAAGCCGGC
GGCAACCCGCAAGCAGATCGAGAAAGCCATCGACATGCTCTGCGCCGCCGAGCGCCCGCTGATCGTCGCCGGTGGCGGCA
TCTACAACGCCGCGGCCGAGGCCTTGCTGGTCGAATTCGCCGAGACCGTGGGCGTGCCGGTGATCCCGACGCTGATGGGC
TGGGGCTCGATCCCCGACGACCATCCGCTGATGGCCGGGATGTGCGGGCTGCAGACCAGCCACCGCTACGGCAACGCGAC
CCTGCTCGAATCCGACTTCGTGCTCGGCATCGGCAACCGCTGGGCCAACCGTCACACCGGCTCCGTGGAGACCTATACCA
AGGGCCGCACCTTCGTCCACGTCGACATCGAGCCGACCCAGATCGGTCGGGTGTTCGCGCCGGACTACGGCATCGTCTCC
GATGCCCGCGCCGCGCTGGAGCTGTTCGTCGACGTGGCCAAGGCGCGCAAGGCCGAAGGCCGTCTGCCGGATCGCCGCGC
CTGGGCCGCCGACTGCCTGGAACGCAAGCGCACGATGTTGCGCAAGACCAACTTCGACAGCGTGCCGATGAAGCCGCAGC
GCGTGTACCAGTGCATGAACAACGCCTTCGGCAAGGACACCTGCTACGTCAGCACCATCGGCCTGTCGCAGATCGCCGCC
GCGCAGTTCCTGCACGTCTACCAGCCGCGCCACTGGATCAACTGCGGCCAGGCCGGTCCGCTCGGCTGGACCATTCCGGC
GGCACTCGGTGTGGTTGCCGCAGACCCGGCGCGCAAGGTGGTGGCGCTGTCCGGCGACTACGACTTCCAGTTCATGATCG
AGGAACTGGCCGTCGGCGCGCAGTTCAAGCTGCCCTACCTGCACATCCTGGTGAACAACGCCTACCTCGGGCTGATCCGC
CAGGCGCAGCGCGGCTTCGACATGGATTACTGCGTGCAGCTGGGCTTCGAGAACATCAATGCCGACCAGAGCGGCATGGA
AGGCTACGGCGTCGATCACGTCGCGGTGGTCGAGGGCCTGGGCTGCAAGGCGCTGCGCGTGTTCAAGCAGGAAGACCTGC
GCCCGGCCATCGAGCAGGCCCAGGCCTGGATGGCCGAACACCGCGTGCCGGTGGTGATCGAGGTGATCCTCGAGCGGGTG
ACCAACATCGCCATGGGCACCGAGATCGACGCGATCAACGAGTTCGAGGCGCTGGCCGCAGGCCGGGAAGATGCCCCGAC
CGCGGTTGGATTGCTGGACTGA

Upstream 100 bases:

>100_bases
AGCGGAGTTTGCTGGTGCAAATGAGCATTCCGAGCCTGGTTTTAACGCAGCAGGGCCGACGCGCAGCAGATCGTGATTCC
AAGAATCAGGAGGAAGAACA

Downstream 100 bases:

>100_bases
TTCAACCGTAACCAGACGCGTGGAAGACGCTTCGCGGCCCTCCATCCTACGAACACTTCGGACCGTAGGGTGGCTATCGC
GAAGCATATCCACCGCAAAC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEGYTRAKPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLI
DLPFDVQMAEIEFDVDTYEPLPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMG
WGSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVHVDIEPTQIGRVFAPDYGIVS
DARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTMLRKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAA
AQFLHVYQPRHWINCGQAGPLGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIR
QAQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQAQAWMAEHRVPVVIEVILERV
TNIAMGTEIDAINEFEALAAGREDAPTAVGLLD

Sequences:

>Translated_593_residues
MARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEGYTRAKPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLI
DLPFDVQMAEIEFDVDTYEPLPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMG
WGSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVHVDIEPTQIGRVFAPDYGIVS
DARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTMLRKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAA
AQFLHVYQPRHWINCGQAGPLGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIR
QAQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQAQAWMAEHRVPVVIEVILERV
TNIAMGTEIDAINEFEALAAGREDAPTAVGLLD
>Mature_592_residues
ARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEGYTRAKPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLID
LPFDVQMAEIEFDVDTYEPLPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMGW
GSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVHVDIEPTQIGRVFAPDYGIVSD
ARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTMLRKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAAA
QFLHVYQPRHWINCGQAGPLGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIRQ
AQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQAQAWMAEHRVPVVIEVILERVT
NIAMGTEIDAINEFEALAAGREDAPTAVGLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=24.1992882562278, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=24.4701348747592, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1786717, Length=587, Percent_Identity=77.6831345826235, Blast_Score=957, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=32.9181494661922, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=33.8842975206612, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=27.348643006263, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=23.5611510791367, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=521, Percent_Identity=24.5681381957774, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=521, Percent_Identity=24.5681381957774, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=559, Percent_Identity=23.0769230769231, Blast_Score=99, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=597, Percent_Identity=29.8157453936348, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=24.5989304812834, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=493, Percent_Identity=21.7038539553753, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=22.0408163265306, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=490, Percent_Identity=22.0408163265306, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.8407079646018, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64434; Mature: 64303

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHC
YTRAKPGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLIDLPFDVQMAEIEFDVDTYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMG
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHC
WGSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFAPDYGIVSDARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTML
EECCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAAAQFLHVYQPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCHHCCCCCCCC
LGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIR
CCEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QAQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQA
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHH
QAWMAEHRVPVVIEVILERVTNIAMGTEIDAINEFEALAAGREDAPTAVGLLD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEECCCC
>Mature Secondary Structure 
ARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHC
YTRAKPGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLIDLPFDVQMAEIEFDVDTYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMG
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHC
WGSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFAPDYGIVSDARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTML
EECCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAAAQFLHVYQPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCHHCCCCCCCC
LGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIR
CCEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QAQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQA
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHH
QAWMAEHRVPVVIEVILERVTNIAMGTEIDAINEFEALAAGREDAPTAVGLLD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]