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The map label for this gene is oprJ [H]

Identifier: 146283432

GI number: 146283432

Start: 3343662

End: 3344795

Strand: Direct

Name: oprJ [H]

Synonym: PST_3105

Alternate gene names: 146283432

Gene position: 3343662-3344795 (Clockwise)

Preceding gene: 146283430

Following gene: 146283438

Centisome position: 73.21

GC content: 68.96

Gene sequence:

>1134_bases
ATGCCCTATTTATTTGCCAGGTTAATACCGAGCCTGGACGCGGTGTTCGGGCTGGAGCGCAGCCAGGACCTCGGCCCCCT
TGGCCTGACCCGCACCACCGCCGCGCAACCCGGCCTGCAGTTCGCCTACGAGGTCGATCTCTGGGGCCGCCTGGCCAGCC
TGCGCGAGGCGGCAGAGTTGCAGTTCCAGGCCAGCGAAGCCGAGCAGCAGACCCTGCGCCTGGCGCTGGCCGCGACCGTC
GCACAGGGCTATGTGGCGCTGCTGTCACTGGATCGTCAGCTGCTGCTGACCCATGAGACGGTGCGGTCCCGTCGCGCTGC
CCTGGCGGTTGCCGAGGACCGGTCGCGCATGGGCTACACCTCCGAACTGGAGCAGACCCAGGCGCGTTCGGAGTACGAGG
CGGCCGCGCAACTGGTGCCGCAGCTGGAGCAGGCCATCCTCGAGCAGGAACACGCCCTGCGACGCCTGACCGATGACCCG
CCCGGGCCGGTGCCACGGGGCACGCTTGCCGCACTGCAAGCTCCAGCGGTGCCAGGCAGCCTGCCTTCGGAGCTGCTGCG
GCAGCGGCCGGATCTGTACCAGGCCGAGCTGCAGCTGGCCGCCAGTGACCGTTTCCTCGACAGCGAGCGCGACCGCTTTC
TGCCGCGGGTGCAACTCTCGGCAAGCCTGGGCCGGCTATACGTCGATGCGCTGGATTACGACCCGGTGAAGGTCTGGAGC
CTCGGCGCCAGCGTACTGGCCCCGCTGTTCGATGGCGAGCGGCTGGAGGCCGGCGTGGCGGTGGCCACGGCCGAGCGCAA
TCAGGCTGCCTACGCCTATCGCAGGGCGGCGCTCAATGCTTTCAGCGAGGTGGAGAACGCGCTATCCGGGCTGGACAGCC
TCGCCCGTCAGAGCCGCCGTCTTGAAGCGCGCCGCGAAGTGCTGGCGCGCTCGCTGGCGATCGCCGAGGACCGCTATCAC
GGCGGTTATTCCTCTTATCTGGAGGCGCTGGATGCGCAGCGCAATCTGTTCGATACCCAACTGGCCGAGGTGCGGCTGCA
GGAGGCGCAGCTGAACAGATTGATCGAGCTCTACCGCGCCCTGGGTGGCGGCTGGCGGGCGCCGGAGCATGCTGCTACCG
AGCGCGCGGAGTGA

Upstream 100 bases:

>100_bases
AACCCTGCTCACGCATGCGTAGGGCCGTGGAGCGCTTTTCACGTTGTTCTTGGGGGCTGAGTTTACGGGCATCTATTTTC
ATGGAGACAGATTATCTAAT

Downstream 100 bases:

>100_bases
GTGCGGGCATAAAAAAACGCCAGCAGGTGCTGGCGTTTCGTGCAGACGGGTAACGCGCGATCAGCCGCGTGCGCGAGCGG
CGTCACGCAGTGCCTTGACC

Product: outer membrane protein OprJ precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MPYLFARLIPSLDAVFGLERSQDLGPLGLTRTTAAQPGLQFAYEVDLWGRLASLREAAELQFQASEAEQQTLRLALAATV
AQGYVALLSLDRQLLLTHETVRSRRAALAVAEDRSRMGYTSELEQTQARSEYEAAAQLVPQLEQAILEQEHALRRLTDDP
PGPVPRGTLAALQAPAVPGSLPSELLRQRPDLYQAELQLAASDRFLDSERDRFLPRVQLSASLGRLYVDALDYDPVKVWS
LGASVLAPLFDGERLEAGVAVATAERNQAAYAYRRAALNAFSEVENALSGLDSLARQSRRLEARREVLARSLAIAEDRYH
GGYSSYLEALDAQRNLFDTQLAEVRLQEAQLNRLIELYRALGGGWRAPEHAATERAE

Sequences:

>Translated_377_residues
MPYLFARLIPSLDAVFGLERSQDLGPLGLTRTTAAQPGLQFAYEVDLWGRLASLREAAELQFQASEAEQQTLRLALAATV
AQGYVALLSLDRQLLLTHETVRSRRAALAVAEDRSRMGYTSELEQTQARSEYEAAAQLVPQLEQAILEQEHALRRLTDDP
PGPVPRGTLAALQAPAVPGSLPSELLRQRPDLYQAELQLAASDRFLDSERDRFLPRVQLSASLGRLYVDALDYDPVKVWS
LGASVLAPLFDGERLEAGVAVATAERNQAAYAYRRAALNAFSEVENALSGLDSLARQSRRLEARREVLARSLAIAEDRYH
GGYSSYLEALDAQRNLFDTQLAEVRLQEAQLNRLIELYRALGGGWRAPEHAATERAE
>Mature_376_residues
PYLFARLIPSLDAVFGLERSQDLGPLGLTRTTAAQPGLQFAYEVDLWGRLASLREAAELQFQASEAEQQTLRLALAATVA
QGYVALLSLDRQLLLTHETVRSRRAALAVAEDRSRMGYTSELEQTQARSEYEAAAQLVPQLEQAILEQEHALRRLTDDPP
GPVPRGTLAALQAPAVPGSLPSELLRQRPDLYQAELQLAASDRFLDSERDRFLPRVQLSASLGRLYVDALDYDPVKVWSL
GASVLAPLFDGERLEAGVAVATAERNQAAYAYRRAALNAFSEVENALSGLDSLARQSRRLEARREVLARSLAIAEDRYHG
GYSSYLEALDAQRNLFDTQLAEVRLQEAQLNRLIELYRALGGGWRAPEHAATERAE

Specific function: The outer membrane component of an organic solvent efflux pump. Involved in export of a number of low log POW compounds including hexane (log POW 3.5), toluene (log POW 2.5) and dimethylphthalate (log POW 2.3). The solvent resistance phenotype has been po

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=336, Percent_Identity=29.7619047619048, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367348, Length=340, Percent_Identity=27.3529411764706, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 41707; Mature: 41576

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYLFARLIPSLDAVFGLERSQDLGPLGLTRTTAAQPGLQFAYEVDLWGRLASLREAAEL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
QFQASEAEQQTLRLALAATVAQGYVALLSLDRQLLLTHETVRSRRAALAVAEDRSRMGYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SELEQTQARSEYEAAAQLVPQLEQAILEQEHALRRLTDDPPGPVPRGTLAALQAPAVPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCC
LPSELLRQRPDLYQAELQLAASDRFLDSERDRFLPRVQLSASLGRLYVDALDYDPVKVWS
CHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
LGASVLAPLFDGERLEAGVAVATAERNQAAYAYRRAALNAFSEVENALSGLDSLARQSRR
HHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEARREVLARSLAIAEDRYHGGYSSYLEALDAQRNLFDTQLAEVRLQEAQLNRLIELYRA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGWRAPEHAATERAE
HCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
PYLFARLIPSLDAVFGLERSQDLGPLGLTRTTAAQPGLQFAYEVDLWGRLASLREAAEL
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
QFQASEAEQQTLRLALAATVAQGYVALLSLDRQLLLTHETVRSRRAALAVAEDRSRMGYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SELEQTQARSEYEAAAQLVPQLEQAILEQEHALRRLTDDPPGPVPRGTLAALQAPAVPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCC
LPSELLRQRPDLYQAELQLAASDRFLDSERDRFLPRVQLSASLGRLYVDALDYDPVKVWS
CHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
LGASVLAPLFDGERLEAGVAVATAERNQAAYAYRRAALNAFSEVENALSGLDSLARQSRR
HHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEARREVLARSLAIAEDRYHGGYSSYLEALDAQRNLFDTQLAEVRLQEAQLNRLIELYRA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGWRAPEHAATERAE
HCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9417051; 9852029 [H]