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The map label for this gene is mscM [H]

Identifier: 146283343

GI number: 146283343

Start: 3243673

End: 3244974

Strand: Direct

Name: mscM [H]

Synonym: PST_3013

Alternate gene names: 146283343

Gene position: 3243673-3244974 (Clockwise)

Preceding gene: 146283342

Following gene: 146283346

Centisome position: 71.02

GC content: 63.44

Gene sequence:

>1302_bases
ATGTCCGACGACCTGGCCGGCTGGCTGGAATGGCTAAGAGCCCACCCCGAACTGCAGACGCTGCTCGCCAGCGCCACGCT
GATCTTCGCCGCCTGGCTGTCCAACTGGATCGTCAAGCGCATCCTCGTCCGCGGCCTGTACCACCTGTTGCAGCACACCC
GCGAGAACAAGCTGCAGGACTTCGGCGTCATCAAGCGGCTGTCGAATATCGTCCCGGCACTGGTGCTGTCGATCGGCGTC
ACGACGGTCCCAGGCCTGCCGGAAGGCGCCGTGACCGTGGTGCGCAACGTCTGCGGCGGCTTCATCGTGCTGACCATCGC
ACTGGCCCTGGGCGCGGTGCTGGACATCATCAACCTGATCTACCAGCGCCGGCCGGATGCGCGCCTGCACCCGATCAAGG
GCTATCTGCAGGTGGTGAAGATCGTCGTCTATGCCATCGCGACGATCCTGATCATCGCCACGCTGATCGACCGCTCGCCG
CTGATCCTGCTATCCGGCCTTGGCGCCATGGCGGCGGTGCTGATGCTGATCTTCCAGGACACCCTGCTGTCACTGGTGGC
CAGCGTGCAGATCACCTCCAACGACCTGATCCGCGTCGGCGACTGGGTGGAAATGCCGCAGCTCAATGCCGACGGCGACG
TGATCGACATCGCCCTGCATACCGTCAAGGTGCAGAACTGGGACAAGACGATCACCAGCATTCCCACCAAGCGCTTCATT
TCCGACTCGTTCAAGAACTGGCGCGGCATGCAGGAAAGCGGCGGCCGGCGAATCAAGCGCAGCCTGTACCTGGACCAGCA
GAGCGTGCACTTTCTCAGCCGCGAAGAATGCGAGCGCCTGCATCGCTTCAACCTGCTGGACGAGTACCTGACCGAGAAAA
CCAAGGAAATCGAGGAGTGGAACGCCAAGCTCGCCGAGCGCGGCAAGGAGCCGGTGAACACGCGCCGCATCACCAATATC
GGCAGCTTCCGCGCGTACGTCGAACGCTACCTGCGTAGCCACGGCGGCATCCATCAGAACATGACGCTGATCGTGCGGCA
GCTGAGCCCGACCGCGGACGGCCTGCCGCTGGAGGTCTACTGCTTCACCAATACGGTGGCATGGACGCAGTACGAGGCGA
TCCAGTCGGACATTTTCGATCACCTGCTGGCGATCCTGCCGGAATTCGGCCTGCGCGTGTTCCAGCACCCGAGCGGTGCG
GACATGCGCAATTGGGGCGAATCGCTGACGCAACGCGGCACGCCGGCATTGCCCGAGGCACAAACGCCTCCGTTCGACGC
AGGTGCCGATCGAACCGGCTAA

Upstream 100 bases:

>100_bases
AGCCGCGAGTTCATCCGCTGAGCCGGACGCTGCGAACCGACGGGCCGCGACCACCGTGCGCTGCTGAGGTCGCGCCCGAC
CTTCTGTGATGGAGAACCCC

Downstream 100 bases:

>100_bases
TCGTAGGTTGGCGCTGAGGCACGAAGCAGATGGCGCAGCCTTGAGCACTGCCCATTGCGTCCTCCCTGACGCCAGCGTCG
CGGGCCACGCCTTCAAACCC

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSDDLAGWLEWLRAHPELQTLLASATLIFAAWLSNWIVKRILVRGLYHLLQHTRENKLQDFGVIKRLSNIVPALVLSIGV
TTVPGLPEGAVTVVRNVCGGFIVLTIALALGAVLDIINLIYQRRPDARLHPIKGYLQVVKIVVYAIATILIIATLIDRSP
LILLSGLGAMAAVLMLIFQDTLLSLVASVQITSNDLIRVGDWVEMPQLNADGDVIDIALHTVKVQNWDKTITSIPTKRFI
SDSFKNWRGMQESGGRRIKRSLYLDQQSVHFLSREECERLHRFNLLDEYLTEKTKEIEEWNAKLAERGKEPVNTRRITNI
GSFRAYVERYLRSHGGIHQNMTLIVRQLSPTADGLPLEVYCFTNTVAWTQYEAIQSDIFDHLLAILPEFGLRVFQHPSGA
DMRNWGESLTQRGTPALPEAQTPPFDAGADRTG

Sequences:

>Translated_433_residues
MSDDLAGWLEWLRAHPELQTLLASATLIFAAWLSNWIVKRILVRGLYHLLQHTRENKLQDFGVIKRLSNIVPALVLSIGV
TTVPGLPEGAVTVVRNVCGGFIVLTIALALGAVLDIINLIYQRRPDARLHPIKGYLQVVKIVVYAIATILIIATLIDRSP
LILLSGLGAMAAVLMLIFQDTLLSLVASVQITSNDLIRVGDWVEMPQLNADGDVIDIALHTVKVQNWDKTITSIPTKRFI
SDSFKNWRGMQESGGRRIKRSLYLDQQSVHFLSREECERLHRFNLLDEYLTEKTKEIEEWNAKLAERGKEPVNTRRITNI
GSFRAYVERYLRSHGGIHQNMTLIVRQLSPTADGLPLEVYCFTNTVAWTQYEAIQSDIFDHLLAILPEFGLRVFQHPSGA
DMRNWGESLTQRGTPALPEAQTPPFDAGADRTG
>Mature_432_residues
SDDLAGWLEWLRAHPELQTLLASATLIFAAWLSNWIVKRILVRGLYHLLQHTRENKLQDFGVIKRLSNIVPALVLSIGVT
TVPGLPEGAVTVVRNVCGGFIVLTIALALGAVLDIINLIYQRRPDARLHPIKGYLQVVKIVVYAIATILIIATLIDRSPL
ILLSGLGAMAAVLMLIFQDTLLSLVASVQITSNDLIRVGDWVEMPQLNADGDVIDIALHTVKVQNWDKTITSIPTKRFIS
DSFKNWRGMQESGGRRIKRSLYLDQQSVHFLSREECERLHRFNLLDEYLTEKTKEIEEWNAKLAERGKEPVNTRRITNIG
SFRAYVERYLRSHGGIHQNMTLIVRQLSPTADGLPLEVYCFTNTVAWTQYEAIQSDIFDHLLAILPEFGLRVFQHPSGAD
MRNWGESLTQRGTPALPEAQTPPFDAGADRTG

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=364, Percent_Identity=48.0769230769231, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48643; Mature: 48512

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDLAGWLEWLRAHPELQTLLASATLIFAAWLSNWIVKRILVRGLYHLLQHTRENKLQD
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVIKRLSNIVPALVLSIGVTTVPGLPEGAVTVVRNVCGGFIVLTIALALGAVLDIINLI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRRPDARLHPIKGYLQVVKIVVYAIATILIIATLIDRSPLILLSGLGAMAAVLMLIFQD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TLLSLVASVQITSNDLIRVGDWVEMPQLNADGDVIDIALHTVKVQNWDKTITSIPTKRFI
HHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEHHHHEECCHHHHHHHCCHHHHH
SDSFKNWRGMQESGGRRIKRSLYLDQQSVHFLSREECERLHRFNLLDEYLTEKTKEIEEW
HHHHHHCCCCHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAKLAERGKEPVNTRRITNIGSFRAYVERYLRSHGGIHQNMTLIVRQLSPTADGLPLEVY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEE
CFTNTVAWTQYEAIQSDIFDHLLAILPEFGLRVFQHPSGADMRNWGESLTQRGTPALPEA
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
QTPPFDAGADRTG
CCCCCCCCCCCCC
>Mature Secondary Structure 
SDDLAGWLEWLRAHPELQTLLASATLIFAAWLSNWIVKRILVRGLYHLLQHTRENKLQD
CCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVIKRLSNIVPALVLSIGVTTVPGLPEGAVTVVRNVCGGFIVLTIALALGAVLDIINLI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRRPDARLHPIKGYLQVVKIVVYAIATILIIATLIDRSPLILLSGLGAMAAVLMLIFQD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TLLSLVASVQITSNDLIRVGDWVEMPQLNADGDVIDIALHTVKVQNWDKTITSIPTKRFI
HHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEHHHHEECCHHHHHHHCCHHHHH
SDSFKNWRGMQESGGRRIKRSLYLDQQSVHFLSREECERLHRFNLLDEYLTEKTKEIEEW
HHHHHHCCCCHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAKLAERGKEPVNTRRITNIGSFRAYVERYLRSHGGIHQNMTLIVRQLSPTADGLPLEVY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEE
CFTNTVAWTQYEAIQSDIFDHLLAILPEFGLRVFQHPSGADMRNWGESLTQRGTPALPEA
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
QTPPFDAGADRTG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]