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The map label for this gene is braD [H]

Identifier: 146283318

GI number: 146283318

Start: 3215999

End: 3216922

Strand: Direct

Name: braD [H]

Synonym: PST_2983

Alternate gene names: 146283318

Gene position: 3215999-3216922 (Clockwise)

Preceding gene: 146283317

Following gene: 146283319

Centisome position: 70.41

GC content: 62.01

Gene sequence:

>924_bases
ATGCCTGAGCTTTACCACTACCTACAACAGCTGATCAACGGGCTGACCGTTGGCAGCACCTACGCGCTGATCGCCATCGG
TTACACGATGGTGTACGGCATCATCGGCATGATCAACTTCGCCCACGGCGAGGTGTACATGATCGGCTCGTATGTCGCTT
TCATCGCCATTGTCGGACTGTCCATGATGGGCCTGGATGCCTTGCCTATCCTGATGATCGGCGCCTTCGTCACCGCCATG
ATCGTCACCAGCGCCTACGGCTACAGCATCGAACGGGTGGCCTACCGCCCGCTACGTGGCAGTAACCGCCTGATCCCGCT
GATCTCCGCCATCGGCATGTCCATCTTCCTGCAGAACGTCGTGCTGCTGGCCCAGGACTCCAAGGACAAGGCGATCCCCA
ACCTGATGCCGGGCAACCTGGTCATCGGTGAAAGCACCATGAATGGTGTGGTGATCTCCTACATGCAGATCCTGATCTTC
GTGGTCACCTTCGTCGCCATGTACGGCCTCACGCTGTTCATCTCCCGCTCCCGCCTGGGCCGTGCCTGCCGCGCCTGCGC
CGAGGACCTGAAGATGGCCAACCTGCTGGGCATCAACACCAACGGCATCATCGCGCTGACCTTCGTCATCGGCGCGGCGC
TGGCTGCCATCGCGGCGGTGCTGATCAGCATGCAGTACGGCGTGATCAACCCGCACATCGGCTTCCTCGCCGGCATCAAG
GCATTCACTGCCGCAGTGCTTGGCGGCATCGGCAGCATTCCGGGCGCCATGCTCGGCGGCCTGGTGCTGGGCGTCGCCGA
AGCCTTCGGTGCCGACATCTTCGGCGATCAGTACAAGGACGTCGTGGCCTTCAGCCTGCTCGTGCTCGTGCTGCTGTTCC
GCCCCACCGGCATCCTTGGCCGCCCGGAGGTGGAAAAGGTATGA

Upstream 100 bases:

>100_bases
GTGCCGTACCGCAGTGGCGCCGCGCCAGGGTGTGCGGTGTGTTCGAAGTGGGCAGCGCAGCCAGACCGGATGGAATTGCA
GGGACTTCGGGAGAGCAGTG

Downstream 100 bases:

>100_bases
CCGGCAATCTGCGTATCGCTTTCTTCAGCGCCCTGCTGGTCATCGCCGTGGCGGTGCCGGTGTTCGGCCTGAAGCTCACC
ACCGTCGGTATCCGCGTCGA

Product: branched-chain amino acid transport protein BraD

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPELYHYLQQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIVGLSMMGLDALPILMIGAFVTAM
IVTSAYGYSIERVAYRPLRGSNRLIPLISAIGMSIFLQNVVLLAQDSKDKAIPNLMPGNLVIGESTMNGVVISYMQILIF
VVTFVAMYGLTLFISRSRLGRACRACAEDLKMANLLGINTNGIIALTFVIGAALAAIAAVLISMQYGVINPHIGFLAGIK
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLVLVLLFRPTGILGRPEVEKV

Sequences:

>Translated_307_residues
MPELYHYLQQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIVGLSMMGLDALPILMIGAFVTAM
IVTSAYGYSIERVAYRPLRGSNRLIPLISAIGMSIFLQNVVLLAQDSKDKAIPNLMPGNLVIGESTMNGVVISYMQILIF
VVTFVAMYGLTLFISRSRLGRACRACAEDLKMANLLGINTNGIIALTFVIGAALAAIAAVLISMQYGVINPHIGFLAGIK
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLVLVLLFRPTGILGRPEVEKV
>Mature_306_residues
PELYHYLQQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIVGLSMMGLDALPILMIGAFVTAMI
VTSAYGYSIERVAYRPLRGSNRLIPLISAIGMSIFLQNVVLLAQDSKDKAIPNLMPGNLVIGESTMNGVVISYMQILIFV
VTFVAMYGLTLFISRSRLGRACRACAEDLKMANLLGINTNGIIALTFVIGAALAAIAAVLISMQYGVINPHIGFLAGIKA
FTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLVLVLLFRPTGILGRPEVEKV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=306, Percent_Identity=67.3202614379085, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32543; Mature: 32411

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELYHYLQQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIVGL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
SMMGLDALPILMIGAFVTAMIVTSAYGYSIERVAYRPLRGSNRLIPLISAIGMSIFLQNV
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VLLAQDSKDKAIPNLMPGNLVIGESTMNGVVISYMQILIFVVTFVAMYGLTLFISRSRLG
HHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACRACAEDLKMANLLGINTNGIIALTFVIGAALAAIAAVLISMQYGVINPHIGFLAGIK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLVLVLLFRPTGILG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPEVEKV
CCCCCCC
>Mature Secondary Structure 
PELYHYLQQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIVGL
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
SMMGLDALPILMIGAFVTAMIVTSAYGYSIERVAYRPLRGSNRLIPLISAIGMSIFLQNV
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VLLAQDSKDKAIPNLMPGNLVIGESTMNGVVISYMQILIFVVTFVAMYGLTLFISRSRLG
HHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACRACAEDLKMANLLGINTNGIIALTFVIGAALAAIAAVLISMQYGVINPHIGFLAGIK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLVLVLLFRPTGILG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPEVEKV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]