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The map label for this gene is braC [H]

Identifier: 146283317

GI number: 146283317

Start: 3214625

End: 3215743

Strand: Direct

Name: braC [H]

Synonym: PST_2982

Alternate gene names: 146283317

Gene position: 3214625-3215743 (Clockwise)

Preceding gene: 146283315

Following gene: 146283318

Centisome position: 70.38

GC content: 61.48

Gene sequence:

>1119_bases
ATGAAGACCAACCAGCGTCTTTCGAAAATTTTCCTGGCAATGGCACTGACCGGCGCAGCCAGCTACAGCCTGGCCGCCGA
CACGATCCGCATCGGATTGGCAGGGCCGGTAACCGGGCCGGTGGCGCAGTACGGGGACATGCAGTTCATCGGGGCCGAAA
TGGCCATCGAGCAGATCAACAAGGCCGGCGGGGTGAACGGCGCGCAACTGCAGGGCGTTCGCTACGACGATGCCTGCGAT
CCGAAACAGGCCGTGGCGGTGGCCAACAAGATCGTCAATGACAACGTCAAGTTCGTCGTTGGCCACCTCTGCTCCAGCTC
CACTCAGCCGGCGTCCGATATCTACGAGGACGAAGGCATCCTGATGATCACCGCGGCGTCCACCAGCCCGGACATCACCT
CCCGCGGTTACGAGCTGATCTTCCGCACCATCGGCCTGGACAGCCTGCAGGGTCCGACCGCCGGCAACTTCATCGCCGAT
CACGTCAAACCGAAGAACGTCGCCGTCATCCACGACAAGCAGCAGTACGGCGAAGGCATCGCCACGGCCGTCAAGCAGAC
CCTTGAAAGCAAGAACATCAAGGTCGGCCTGTTCGAAGGCATCAATGCCGGTGACAAGGACTTCTCCTCGCTGATCGCCA
AGCTCAAGCGCGAAGGCGTCGACTTCGTCTACTACGGCGGCTACCACCCGGAGCTGGGCCTGCTGCTGCGTCAGTCCAAG
GAAAAAGGTCTGAACGTGCGCTTCATGGGTCCGGAAGGCGTCGGCAACTCGGAAATCTCCGCCATCGCCGGCCCGGCATC
CGAAGGCATGTACGTGACGCTGCCGAAGTCCTTCGACCAGGATCCACGCAACAAGGAACTGGTGGATGCCTTCAAGGCCA
AGAAGCAGGACCCGAGCGGCCCGTTCGTGTTCCCCGCCTACGCCGCGGTTCAGGTCATCGCAGAAGGCATCGAGAAGGCT
GGCAGCACCGACACCGACAAGGTCGCCGAAGCGCTGCGCAGCAACACCTTCGACACTCCCACCGGCAACCTCGCGTTCGA
TGAAAAGGGCGACCTGAAGGACTTCAACTTCGTCGTCTACGAATGGCACCAGGACGGCACCAAGACCGAAGCCAAGTAA

Upstream 100 bases:

>100_bases
CACTGTGCCGGTACAGTTGCCGGAGCGTTTCGGAATGTTCGCCTCTTAACGGCACTCCGGCCCGCAGGATTCAAACAATA
ACGATGCAAGTGGAGCACTT

Downstream 100 bases:

>100_bases
CTTTCATCAGCCTGGCAAAGCCCACACTCGCTTGTGGGCTTTGTTTTAGCGGGCGGCCCTGCCACAAGCACCGGGCCGCC
TTGCCATACCTGTAATCCAA

Product: branched-chain amino acid transport protein BraC

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKTNQRLSKIFLAMALTGAASYSLAADTIRIGLAGPVTGPVAQYGDMQFIGAEMAIEQINKAGGVNGAQLQGVRYDDACD
PKQAVAVANKIVNDNVKFVVGHLCSSSTQPASDIYEDEGILMITAASTSPDITSRGYELIFRTIGLDSLQGPTAGNFIAD
HVKPKNVAVIHDKQQYGEGIATAVKQTLESKNIKVGLFEGINAGDKDFSSLIAKLKREGVDFVYYGGYHPELGLLLRQSK
EKGLNVRFMGPEGVGNSEISAIAGPASEGMYVTLPKSFDQDPRNKELVDAFKAKKQDPSGPFVFPAYAAVQVIAEGIEKA
GSTDTDKVAEALRSNTFDTPTGNLAFDEKGDLKDFNFVVYEWHQDGTKTEAK

Sequences:

>Translated_372_residues
MKTNQRLSKIFLAMALTGAASYSLAADTIRIGLAGPVTGPVAQYGDMQFIGAEMAIEQINKAGGVNGAQLQGVRYDDACD
PKQAVAVANKIVNDNVKFVVGHLCSSSTQPASDIYEDEGILMITAASTSPDITSRGYELIFRTIGLDSLQGPTAGNFIAD
HVKPKNVAVIHDKQQYGEGIATAVKQTLESKNIKVGLFEGINAGDKDFSSLIAKLKREGVDFVYYGGYHPELGLLLRQSK
EKGLNVRFMGPEGVGNSEISAIAGPASEGMYVTLPKSFDQDPRNKELVDAFKAKKQDPSGPFVFPAYAAVQVIAEGIEKA
GSTDTDKVAEALRSNTFDTPTGNLAFDEKGDLKDFNFVVYEWHQDGTKTEAK
>Mature_372_residues
MKTNQRLSKIFLAMALTGAASYSLAADTIRIGLAGPVTGPVAQYGDMQFIGAEMAIEQINKAGGVNGAQLQGVRYDDACD
PKQAVAVANKIVNDNVKFVVGHLCSSSTQPASDIYEDEGILMITAASTSPDITSRGYELIFRTIGLDSLQGPTAGNFIAD
HVKPKNVAVIHDKQQYGEGIATAVKQTLESKNIKVGLFEGINAGDKDFSSLIAKLKREGVDFVYYGGYHPELGLLLRQSK
EKGLNVRFMGPEGVGNSEISAIAGPASEGMYVTLPKSFDQDPRNKELVDAFKAKKQDPSGPFVFPAYAAVQVIAEGIEKA
GSTDTDKVAEALRSNTFDTPTGNLAFDEKGDLKDFNFVVYEWHQDGTKTEAK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=368, Percent_Identity=57.6086956521739, Blast_Score=456, Evalue=1e-130,
Organism=Escherichia coli, GI48994941, Length=364, Percent_Identity=58.5164835164835, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39887; Mature: 39887

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNQRLSKIFLAMALTGAASYSLAADTIRIGLAGPVTGPVAQYGDMQFIGAEMAIEQIN
CCCHHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCHHHCCCCEEHHHHHHHHHHH
KAGGVNGAQLQGVRYDDACDPKQAVAVANKIVNDNVKFVVGHLCSSSTQPASDIYEDEGI
HHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCE
LMITAASTSPDITSRGYELIFRTIGLDSLQGPTAGNFIADHVKPKNVAVIHDKQQYGEGI
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEEECHHHHCCHH
ATAVKQTLESKNIKVGLFEGINAGDKDFSSLIAKLKREGVDFVYYGGYHPELGLLLRQSK
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
EKGLNVRFMGPEGVGNSEISAIAGPASEGMYVTLPKSFDQDPRNKELVDAFKAKKQDPSG
HCCCEEEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCC
PFVFPAYAAVQVIAEGIEKAGSTDTDKVAEALRSNTFDTPTGNLAFDEKGDLKDFNFVVY
CEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEE
EWHQDGTKTEAK
EEECCCCCCCCC
>Mature Secondary Structure
MKTNQRLSKIFLAMALTGAASYSLAADTIRIGLAGPVTGPVAQYGDMQFIGAEMAIEQIN
CCCHHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCHHHCCCCEEHHHHHHHHHHH
KAGGVNGAQLQGVRYDDACDPKQAVAVANKIVNDNVKFVVGHLCSSSTQPASDIYEDEGI
HHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCE
LMITAASTSPDITSRGYELIFRTIGLDSLQGPTAGNFIADHVKPKNVAVIHDKQQYGEGI
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEEECHHHHCCHH
ATAVKQTLESKNIKVGLFEGINAGDKDFSSLIAKLKREGVDFVYYGGYHPELGLLLRQSK
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
EKGLNVRFMGPEGVGNSEISAIAGPASEGMYVTLPKSFDQDPRNKELVDAFKAKKQDPSG
HCCCEEEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCC
PFVFPAYAAVQVIAEGIEKAGSTDTDKVAEALRSNTFDTPTGNLAFDEKGDLKDFNFVVY
CEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEE
EWHQDGTKTEAK
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]