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The map label for this gene is tilS [H]

Identifier: 146283237

GI number: 146283237

Start: 3124036

End: 3124506

Strand: Direct

Name: tilS [H]

Synonym: PST_2902

Alternate gene names: 146283237

Gene position: 3124036-3124506 (Clockwise)

Preceding gene: 146283236

Following gene: 146283238

Centisome position: 68.4

GC content: 67.09

Gene sequence:

>471_bases
ATGCCTCTCGATGATCTCGCCCTGCTGCGCAAAAGCTTCGACGTCGCCCGCCGCGCGCTGGAAAACGGCACCCATCCGTT
TGGCGCCATCCTGGTCGGGCCGGACGGTGAGGTGCTGCTGGAACAGGGCAACGCCTACATGCCCGACCACGACATGACCG
GCCATGCCGAGCGGGTGCTGATGACCCGAGCCTCGACCCGCTACACGCCGGAATTCCTCTCGCGCTGCACCATGTACACG
TCCGCCGAACCCTGCGCCATGTGCGCGGGTGCCGCCTACTGGGTCGGTCTTGGGCGCGTGGTCTACGGCCTTTCCGAGCG
CAGCCTGAAGGACCTCACCGGTAACCATCCGGAAAACCCGACGCTGGATTTGCCGTGCCGCATCGTCTTCGACGCCGGCC
AGCGCAAGGTCGAGGTGCTCGGCCCGCTGCTGGAGGACGAGGCGGCCGCCCTGCACGAAGGCATCTGGTAG

Upstream 100 bases:

>100_bases
CCACCTGATCGGGCTGCTGCAGAAGGTCTGCTTTCCCTGGAGCCTGCCCAAGGCGGGGCACTGAAGCCCACCACCCCTTC
ATCTCAGTCTGGATATCGCC

Downstream 100 bases:

>100_bases
GCAGCGCCAGAATCAGGCAAGCGCCCGGCAGGATCGCCATAACGCCGGCACCGTCCTCCATCGCAGTATTCGAACCACGG
CGTCACCGACGTCGCCCGAA

Product: cytidine/deoxycytidylate deaminase family protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MPLDDLALLRKSFDVARRALENGTHPFGAILVGPDGEVLLEQGNAYMPDHDMTGHAERVLMTRASTRYTPEFLSRCTMYT
SAEPCAMCAGAAYWVGLGRVVYGLSERSLKDLTGNHPENPTLDLPCRIVFDAGQRKVEVLGPLLEDEAAALHEGIW

Sequences:

>Translated_156_residues
MPLDDLALLRKSFDVARRALENGTHPFGAILVGPDGEVLLEQGNAYMPDHDMTGHAERVLMTRASTRYTPEFLSRCTMYT
SAEPCAMCAGAAYWVGLGRVVYGLSERSLKDLTGNHPENPTLDLPCRIVFDAGQRKVEVLGPLLEDEAAALHEGIW
>Mature_155_residues
PLDDLALLRKSFDVARRALENGTHPFGAILVGPDGEVLLEQGNAYMPDHDMTGHAERVLMTRASTRYTPEFLSRCTMYTS
AEPCAMCAGAAYWVGLGRVVYGLSERSLKDLTGNHPENPTLDLPCRIVFDAGQRKVEVLGPLLEDEAAALHEGIW

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17554608, Length=123, Percent_Identity=32.520325203252, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00383 dCMP_cyt_deam_1; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 17130; Mature: 16999

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDDLALLRKSFDVARRALENGTHPFGAILVGPDGEVLLEQGNAYMPDHDMTGHAERVL
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHH
MTRASTRYTPEFLSRCTMYTSAEPCAMCAGAAYWVGLGRVVYGLSERSLKDLTGNHPENP
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
TLDLPCRIVFDAGQRKVEVLGPLLEDEAAALHEGIW
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLDDLALLRKSFDVARRALENGTHPFGAILVGPDGEVLLEQGNAYMPDHDMTGHAERVL
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHH
MTRASTRYTPEFLSRCTMYTSAEPCAMCAGAAYWVGLGRVVYGLSERSLKDLTGNHPENP
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
TLDLPCRIVFDAGQRKVEVLGPLLEDEAAALHEGIW
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA