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The map label for this gene is proS

Identifier: 146283154

GI number: 146283154

Start: 3041113

End: 3042828

Strand: Direct

Name: proS

Synonym: PST_2819

Alternate gene names: 146283154

Gene position: 3041113-3042828 (Clockwise)

Preceding gene: 146283153

Following gene: 146283155

Centisome position: 66.58

GC content: 61.19

Gene sequence:

>1716_bases
ATGCGCACCAGTCAGTTCCTGCTCTCGACCCTCAAAGAAACCCCTTCCGATGCGGTGGTGATCAGTCACCAGCTGATGCT
GCGTGCCGGAATGATCCGCAAGCTGGCGTCCGGCCTCTACACCTGGATGCCGATGGGGCTGCGAGCGCTACGCAAGGCCG
AGGCCATCGTTCGCGAGGAAATGAACAAGGCAGGCGCGCTGGAAGTCCTGATGCCCGCCATCCAGCCGGCCGAACTTTGG
CAGGAATCCGGTCGCTGGGAGCAATACGGACCGGAGCTGCTGCGCCTGAAGGATCGCCATGACCGTGAGTTCTGTGTCGG
CCCGACCCATGAGGAAGTCATCACCGATCTGGCGCGCAATGAGCTGAACAGCTACAAGCAGCTGCCGATCAACTTCTATC
AGATCCAGACCAAGTTCCGTGACGAGATCCGCCCGCGCTTCGGGCTCATGCGTGGGCGCGAGTTCCTGATGAAGGATGCC
TACTCCTTTCATCTGAACCAGGAGTCGCTGCAGGAAACCTACGATCGGATGCATCAGGCCTACTGCAACATCTTCACCCG
CCTAGGCTTGAACTTCCGTCCGGTACAGGCCGATACCGGTTCCATCGGCGGCACTGGCTCCCACGAATTTCATGTACTGG
CCGAATCCGGCGAGGACGATATCGCCTTCAGCGATTCCTCCGACTACGCTGCCAATATCGAGAAGGCCGAAGCCATCCCG
CGCGAAACCGAACGCGCAGCCCCTGGCGAAGAGTTGCGCCTGATCGATACACCGGATGCCAAGACCATCATCGAGCTGGT
CGAGCAGTTCGGCGTGGCCGTCGAGAAAACCGTCAAGACGCTTGTGGTGCACGGCGTCGAGAAAGGCCAGCTGGTCGCCC
TGATCGTGCGCGGCGACCATGAACTGAACGAGATCAAGGCCGCCAATCTGGCACAGGTCGCTAGTCCGCTGGTATTCGCC
TCGGAAGCCGAGATCCGCGCTGCCATCGGCGCCGGCCCCGGTTCACTGGGCCCGCTGAACCTGCCGATTCCTTGTGTGAT
CGACCGCTCTGTCGCACTGATGAGCGACTTCTCCGCCGGCGCCAACATCGATGACAAGCACTATCTCGGCCTGAACTGGG
AGCGCGACCTGCCGTTGCCGCAGGTTGCTGACCTGCGCAACGTGGTCGCTGGCGACCCCAGCCCGGACGGCAAGGGCAGC
CTGGTAATCAAGCGCGGCATCGAAGTCGGTCATATCTTCCAGCTCGGCACCAAGTACAGCGAAGCGATGAACTGCCAGGT
CATGGGCGAGAACGGCAAGCCGGCGACCCTCATCATGGGTTGCTACGGCATCGGGGTTTCCCGCGTGGTCGCTGCGGCTA
TCGAGCAGAACTACGACGAGCGCGGCATTCTCTGGCCGGACGCCCTGGCACCGTTCCAGATTGCGCTGGTACCGATGAAA
TACGAGACCGAGGCGGTTCGCGAAGCTACCGACAAGCTCTACGCCGAGCTGACCGCAGCCGGCTACGAAGTTCTGCTCGA
CGACCGTGACAAAAAGACCAGCCCTGGCGTCAAATTCGCCGACATGGAGCTGATCGGCATCCCGCACCGTATCGTCGTCA
GTGATCGCGGGCTGGCAGACGGCATGCTGGAGTACAAGCATCGCCGCGATGCCCAACCACAGCAGGTAGCCGTTTCAGAT
ATCCTCTCCTTCATCAATTCGCGTGTTCATCGCTGA

Upstream 100 bases:

>100_bases
TTTCCGTCGCCCAAGCGACCGTACCTGTCCTGTACGCCGCACCACGCGCGCCGTACAATGCCGAGCCTAATTCCAGCCTC
TGCAAGGACATAACGGCCAC

Downstream 100 bases:

>100_bases
GCGGAAACCATGCTCAACCGACATCTTGTCGCTTCAACCAGCGCCGCGCTCTGCGCGGCGCTTCTGCTTGGCGGCTGCGC
CAATCACCTGCCTCAGCGCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWMPMGLRALRKAEAIVREEMNKAGALEVLMPAIQPAELW
QESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARNELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDA
YSFHLNQESLQETYDRMHQAYCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIP
RETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDHELNEIKAANLAQVASPLVFA
SEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAGANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGS
LVIKRGIEVGHIFQLGTKYSEAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILWPDALAPFQIALVPMK
YETEAVREATDKLYAELTAAGYEVLLDDRDKKTSPGVKFADMELIGIPHRIVVSDRGLADGMLEYKHRRDAQPQQVAVSD
ILSFINSRVHR

Sequences:

>Translated_571_residues
MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWMPMGLRALRKAEAIVREEMNKAGALEVLMPAIQPAELW
QESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARNELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDA
YSFHLNQESLQETYDRMHQAYCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIP
RETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDHELNEIKAANLAQVASPLVFA
SEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAGANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGS
LVIKRGIEVGHIFQLGTKYSEAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILWPDALAPFQIALVPMK
YETEAVREATDKLYAELTAAGYEVLLDDRDKKTSPGVKFADMELIGIPHRIVVSDRGLADGMLEYKHRRDAQPQQVAVSD
ILSFINSRVHR
>Mature_571_residues
MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWMPMGLRALRKAEAIVREEMNKAGALEVLMPAIQPAELW
QESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARNELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDA
YSFHLNQESLQETYDRMHQAYCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIP
RETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDHELNEIKAANLAQVASPLVFA
SEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAGANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGS
LVIKRGIEVGHIFQLGTKYSEAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILWPDALAPFQIALVPMK
YETEAVREATDKLYAELTAAGYEVLLDDRDKKTSPGVKFADMELIGIPHRIVVSDRGLADGMLEYKHRRDAQPQQVAVSD
ILSFINSRVHR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=48.6725663716814, Blast_Score=233, Evalue=5e-61,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=70.8041958041958, Blast_Score=852, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=233, Percent_Identity=39.9141630901288, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=34.8837209302326, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=538, Percent_Identity=31.2267657992565, Blast_Score=250, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24656200, Length=233, Percent_Identity=39.0557939914163, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PSEU5 (A4VNB4)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173307.1
- ProteinModelPortal:   A4VNB4
- SMR:   A4VNB4
- STRING:   A4VNB4
- GeneID:   5095781
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_2819
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A4VNB4
- ProtClustDB:   PRK09194
- BioCyc:   PSTU379731:PST_2819-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63377; Mature: 63377

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWMPMGLRALRKAEAIVREE
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGALEVLMPAIQPAELWQESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARN
HHHCCCHHHHHHCCCHHHHHHHCCCCHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
ELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDAYSFHLNQESLQETYDRMHQA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIP
HHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHHCCHHHHCCC
RETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDH
CHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
ELNEIKAANLAQVASPLVFASEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAG
CHHHHHHHHHHHHHCCCEEECCCHHHHEECCCCCCCCCCCCCCCEEECCHHHHHHCCCCC
ANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGSLVIKRGIEVGHIFQLGTKYS
CCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEHHHHHCCHHH
EAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILWPDALAPFQIALVPMK
HCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
YETEAVREATDKLYAELTAAGYEVLLDDRDKKTSPGVKFADMELIGIPHRIVVSDRGLAD
CHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCHH
GMLEYKHRRDAQPQQVAVSDILSFINSRVHR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWMPMGLRALRKAEAIVREE
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGALEVLMPAIQPAELWQESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARN
HHHCCCHHHHHHCCCHHHHHHHCCCCHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
ELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDAYSFHLNQESLQETYDRMHQA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIP
HHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHHCCHHHHCCC
RETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDH
CHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
ELNEIKAANLAQVASPLVFASEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAG
CHHHHHHHHHHHHHCCCEEECCCHHHHEECCCCCCCCCCCCCCCEEECCHHHHHHCCCCC
ANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGSLVIKRGIEVGHIFQLGTKYS
CCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEHHHHHCCHHH
EAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILWPDALAPFQIALVPMK
HCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
YETEAVREATDKLYAELTAAGYEVLLDDRDKKTSPGVKFADMELIGIPHRIVVSDRGLAD
CHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCHH
GMLEYKHRRDAQPQQVAVSDILSFINSRVHR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA