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The map label for this gene is oprF [H]

Identifier: 146283087

GI number: 146283087

Start: 2973982

End: 2974764

Strand: Direct

Name: oprF [H]

Synonym: PST_2748

Alternate gene names: 146283087

Gene position: 2973982-2974764 (Clockwise)

Preceding gene: 146283086

Following gene: 146283100

Centisome position: 65.11

GC content: 64.62

Gene sequence:

>783_bases
ATGCACAAGCTCGTAACCCTACCGACTCTTCTGGCTCTGAGCATTGGCCTGGTGGCCTGCGCCAACCAGCCCAATCAGAA
CCTCGAAGACGCTCGCAGCAGCTACTCCGCACTGCAGGCTGACTCTCGCGCCAGCAAGCTGGCCGCGCTGGAGACCCAGG
ACGCCGGCAACATGCTGGACAAGGCCAACAAGGCCTATCTGAACGATGCCGACGAGAAGACCGTGGACCAACTGGCCTAC
CTGACCAAGCGCCGCATCGAGCTGGCCGAACAGACCATTGCCCTGCGCAGCGCCGAAGAGGAACTGGGCAAGACCTCGGC
ACAGCGCGCCGAGGCGCGTCTGGAGGCGCGCGAGGCGCAGATCCGCAAGTTGCAGGAAGACATGCAGGCCAAGCAGACCG
AACGCGGCACCCTGGTGACCTTTGGCGACGTGCTGTTCGACTACGACCGCGCCGAGCTCAAGCCTGGCGGCATGCGCAGC
GTGCAGAAGCTCGCCGACTTCCTCAACGACAACCCGGAGCGCAAGGTGATCGTCGAGGGCTACACCGACAGTACTGGTTC
GGCCGAGTACAACCAGCGTCTGTCCGAGCGCCGTGCCGATGCGGTTCGCATGGCGCTGGTGAAGATGGGTGTGGACCCGC
AGCGCATCGTCTCCCAGGGCTACGGCAAGGAGTATCCGGTGGCCAGCAACAGCGATTCGGCCGGTCGTGCCATGAACCGC
CGGGTCGAGGTGACGATCTCCGACGACAACAAGCCGGTGGCGCCCCGCTCGTCGATGCGATGA

Upstream 100 bases:

>100_bases
AGCAGGGAATCCGTGAGCTGCGCGAAGAAGGCATGCGCAACGTTCGCTGAGCCCGCATCAGGTTCGACATTCCATTTATT
GCACAAAGGATGAATTCGTC

Downstream 100 bases:

>100_bases
TCAGCCCCGCGTAACGAACGCTCCCGACGCAGCGCTGCGTCGGGAGCGTTTTCTTATGTCCGCGCCCGCGGGCAGAGCCG
TCCGCTCAGCGCAGCAGCCC

Product: OmpA family protein

Products: NA

Alternate protein names: Root adhesin [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MHKLVTLPTLLALSIGLVACANQPNQNLEDARSSYSALQADSRASKLAALETQDAGNMLDKANKAYLNDADEKTVDQLAY
LTKRRIELAEQTIALRSAEEELGKTSAQRAEARLEAREAQIRKLQEDMQAKQTERGTLVTFGDVLFDYDRAELKPGGMRS
VQKLADFLNDNPERKVIVEGYTDSTGSAEYNQRLSERRADAVRMALVKMGVDPQRIVSQGYGKEYPVASNSDSAGRAMNR
RVEVTISDDNKPVAPRSSMR

Sequences:

>Translated_260_residues
MHKLVTLPTLLALSIGLVACANQPNQNLEDARSSYSALQADSRASKLAALETQDAGNMLDKANKAYLNDADEKTVDQLAY
LTKRRIELAEQTIALRSAEEELGKTSAQRAEARLEAREAQIRKLQEDMQAKQTERGTLVTFGDVLFDYDRAELKPGGMRS
VQKLADFLNDNPERKVIVEGYTDSTGSAEYNQRLSERRADAVRMALVKMGVDPQRIVSQGYGKEYPVASNSDSAGRAMNR
RVEVTISDDNKPVAPRSSMR
>Mature_260_residues
MHKLVTLPTLLALSIGLVACANQPNQNLEDARSSYSALQADSRASKLAALETQDAGNMLDKANKAYLNDADEKTVDQLAY
LTKRRIELAEQTIALRSAEEELGKTSAQRAEARLEAREAQIRKLQEDMQAKQTERGTLVTFGDVLFDYDRAELKPGGMRS
VQKLADFLNDNPERKVIVEGYTDSTGSAEYNQRLSERRADAVRMALVKMGVDPQRIVSQGYGKEYPVASNSDSAGRAMNR
RVEVTISDDNKPVAPRSSMR

Specific function: Known to stabilize the outer membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=137, Percent_Identity=41.6058394160584, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786962, Length=98, Percent_Identity=41.8367346938776, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787191, Length=126, Percent_Identity=38.0952380952381, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=37.1428571428571, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 28784; Mature: 28784

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLVTLPTLLALSIGLVACANQPNQNLEDARSSYSALQADSRASKLAALETQDAGNMLD
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KANKAYLNDADEKTVDQLAYLTKRRIELAEQTIALRSAEEELGKTSAQRAEARLEAREAQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKLQEDMQAKQTERGTLVTFGDVLFDYDRAELKPGGMRSVQKLADFLNDNPERKVIVEG
HHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEC
YTDSTGSAEYNQRLSERRADAVRMALVKMGVDPQRIVSQGYGKEYPVASNSDSAGRAMNR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCC
RVEVTISDDNKPVAPRSSMR
EEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MHKLVTLPTLLALSIGLVACANQPNQNLEDARSSYSALQADSRASKLAALETQDAGNMLD
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KANKAYLNDADEKTVDQLAYLTKRRIELAEQTIALRSAEEELGKTSAQRAEARLEAREAQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKLQEDMQAKQTERGTLVTFGDVLFDYDRAELKPGGMRSVQKLADFLNDNPERKVIVEG
HHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEC
YTDSTGSAEYNQRLSERRADAVRMALVKMGVDPQRIVSQGYGKEYPVASNSDSAGRAMNR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCC
RVEVTISDDNKPVAPRSSMR
EEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1538702 [H]