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The map label for this gene is dapE

Identifier: 146283029

GI number: 146283029

Start: 2915251

End: 2916399

Strand: Direct

Name: dapE

Synonym: PST_2690

Alternate gene names: 146283029

Gene position: 2915251-2916399 (Clockwise)

Preceding gene: 146283025

Following gene: 146283030

Centisome position: 63.83

GC content: 67.01

Gene sequence:

>1149_bases
ATGACCGCCCCCGCCCTCTCCCCGACCCTCGAACTGGCCTGCGAGCTGATCAACCGCCCCTCCGTGACGCCGCTGGACGA
AGGCTGCCAGCAGCTGATGAGCCAGCGCCTGGCGGCTTGCGGCTTCGCAATCGAGCCGATGCACATCGAGGACGTGGAGA
ACTTCTGGGCCATTCGCGGCAACGAAGGCCCGGTGCTGTGCTTCGCCGGCCATACCGACGTGGTTCCCACCGGCCCGCTG
CAGGCCTGGCAGAACCCGCCTTTCAGTGCGCGCATCGACGAGCAGGGCATGCTCCACGGTCGCGGCGCGGCAGACATGAA
AGGCAGCCTGGCCGCCATGGTCGTGGCAGTGGAACGCTTCACCGCCGACCATCCGGACCACAAGGGACAGATCGCCTTCC
TGATCACCAGCGATGAGGAAGGCCCGGCGCACCACGGTACCAAGGCGGTGGTCGAACGCCTGCGCGAGCGCGGCCAGCGC
CTGGACTGGTGCATCGTCGGCGAACCCTCGAGCACCAGCCTGGTGGGCGACGTGGTGAAGAACGGTCGCCGCGGCTCGCT
GGGCGGCACGCTCACCGTTCGCGGTCAGCAGGGCCATGTGGCCTACCCTCATCTGGCGAAGAACCCGATCCACCTGGCCG
CACCGGCACTGGCCGAGCTGGCTGCCGAACACTGGGACGATGGCAATGCGTTCTTCCCGCCGACCAGCTTCCAGATCTCC
AACCTGAACGCCGGCACCGGCGCCACCAACGTCATCCCCGGCACGCTGGAAGCGGTATTCAACTTCCGCTTCTCCACCGA
GTCGACCGTCGAAGGCTTGCAGCAACGCACCGCGGCGATCCTCGACAAGCACGGTCTGGACTGGAGCATCGACTGGGCGC
TGTCCGGCCTGCCGTTCCTCACCGAGCCGGGTGACCTGCTCGACGGCGTGGCCAAGGCGATCCGCAGCGTCACCGGCCGC
GAAACCACGCCGTCCACCAGCGGCGGCACCTCGGACGGACGCTTTATCGCCACCCTGGGTACCCAGGTGGTCGAGCTCGG
CCCGGTCAATGCCACCATCCACCAAGTCGACGAGCACATTCTAGCCAGCGATCTCGATGTGCTGACCGACATCTACTACC
AGACCCTGGTGAACCTGCTGGCATGCTGA

Upstream 100 bases:

>100_bases
TTATAAGACCTCGCCGCGTGCCGACCACTGCATCCGGCCGTCACCGCAGCCCCGGCTTTGCTTTAATATGGCGCCCTTTC
CCCGCCGTACCGGAGCCGTG

Downstream 100 bases:

>100_bases
TCTGCCCGATCTGCCAGGCGCCGCTCAGCGCGGTGGAAAACGGCGTGGCCTGCCCGGCCAACCACCGTTTCGACCGCGCG
CGCCAGGGCTACCTGAACCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTAPALSPTLELACELINRPSVTPLDEGCQQLMSQRLAACGFAIEPMHIEDVENFWAIRGNEGPVLCFAGHTDVVPTGPL
QAWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERFTADHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQR
LDWCIVGEPSSTSLVGDVVKNGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQIS
NLNAGTGATNVIPGTLEAVFNFRFSTESTVEGLQQRTAAILDKHGLDWSIDWALSGLPFLTEPGDLLDGVAKAIRSVTGR
ETTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHILASDLDVLTDIYYQTLVNLLAC

Sequences:

>Translated_382_residues
MTAPALSPTLELACELINRPSVTPLDEGCQQLMSQRLAACGFAIEPMHIEDVENFWAIRGNEGPVLCFAGHTDVVPTGPL
QAWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERFTADHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQR
LDWCIVGEPSSTSLVGDVVKNGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQIS
NLNAGTGATNVIPGTLEAVFNFRFSTESTVEGLQQRTAAILDKHGLDWSIDWALSGLPFLTEPGDLLDGVAKAIRSVTGR
ETTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHILASDLDVLTDIYYQTLVNLLAC
>Mature_381_residues
TAPALSPTLELACELINRPSVTPLDEGCQQLMSQRLAACGFAIEPMHIEDVENFWAIRGNEGPVLCFAGHTDVVPTGPLQ
AWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERFTADHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQRL
DWCIVGEPSSTSLVGDVVKNGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQISN
LNAGTGATNVIPGTLEAVFNFRFSTESTVEGLQQRTAAILDKHGLDWSIDWALSGLPFLTEPGDLLDGVAKAIRSVTGRE
TTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHILASDLDVLTDIYYQTLVNLLAC

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI4501901, Length=330, Percent_Identity=23.9393939393939, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788816, Length=374, Percent_Identity=59.3582887700535, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=225, Percent_Identity=28.4444444444444, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24649212, Length=331, Percent_Identity=25.0755287009063, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24649210, Length=329, Percent_Identity=25.531914893617, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_PSEU5 (A4VMY9)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173182.1
- ProteinModelPortal:   A4VMY9
- STRING:   A4VMY9
- MEROPS:   M20.010
- GeneID:   5095652
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_2690
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A4VMY9
- ProtClustDB:   PRK13009
- BioCyc:   PSTU379731:PST_2690-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40830; Mature: 40699

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00092 N6_MTASE

Important sites: ACT_SITE 73-73 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPALSPTLELACELINRPSVTPLDEGCQQLMSQRLAACGFAIEPMHIEDVENFWAIRG
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEC
NEGPVLCFAGHTDVVPTGPLQAWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERF
CCCCEEEECCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
TADHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQRLDWCIVGEPSSTSLVGDVVK
HCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
NGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQIS
CCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEE
NLNAGTGATNVIPGTLEAVFNFRFSTESTVEGLQQRTAAILDKHGLDWSIDWALSGLPFL
CCCCCCCCCCCCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCC
TEPGDLLDGVAKAIRSVTGRETTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCHHHHHHHHHH
LASDLDVLTDIYYQTLVNLLAC
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAPALSPTLELACELINRPSVTPLDEGCQQLMSQRLAACGFAIEPMHIEDVENFWAIRG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEC
NEGPVLCFAGHTDVVPTGPLQAWQNPPFSARIDEQGMLHGRGAADMKGSLAAMVVAVERF
CCCCEEEECCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
TADHPDHKGQIAFLITSDEEGPAHHGTKAVVERLRERGQRLDWCIVGEPSSTSLVGDVVK
HCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
NGRRGSLGGTLTVRGQQGHVAYPHLAKNPIHLAAPALAELAAEHWDDGNAFFPPTSFQIS
CCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEE
NLNAGTGATNVIPGTLEAVFNFRFSTESTVEGLQQRTAAILDKHGLDWSIDWALSGLPFL
CCCCCCCCCCCCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCC
TEPGDLLDGVAKAIRSVTGRETTPSTSGGTSDGRFIATLGTQVVELGPVNATIHQVDEHI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCHHHHHHHHHH
LASDLDVLTDIYYQTLVNLLAC
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA