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The map label for this gene is mnmC

Identifier: 146283014

GI number: 146283014

Start: 2901685

End: 2903667

Strand: Direct

Name: mnmC

Synonym: PST_2675

Alternate gene names: 146283014

Gene position: 2901685-2903667 (Clockwise)

Preceding gene: 146283013

Following gene: 146283015

Centisome position: 63.53

GC content: 68.03

Gene sequence:

>1983_bases
ATGAACGATCACCCCGCCCAAGACGCCTTCCAGCACGCCGAACTCGACTGGGACGAGAACGGCCTGCCGCAGTCCCGCCA
CTATGGCGATGTGTATTTCTCCCGCGCCAGCGGGCTGGCCGAGACCGAGCACGTATTCCTGGCGCAGAACGATCTGCCCC
GCCGCTTCGCCGCCATGCAGCCCGACGAGTGTCTGGTCATCGGTGAAACCGGCTTCGGCACAGGGCTGAATTTCCTCTGC
GCCTGGCAACTGTTCGAACGCAGCGCCAGTGCCCGGGCGCGGCTGCATTTCGTAAGCGTCGAAAAGCACCCGCTAACCTT
CGCCGACATGCAGCGCGCGCTGGCGCTGTGGCCGGAGCTGCAGGCCCAGGCAGAGCAACTGCTCGAACAATACGTCGCGC
TCAATCCGGGCTATCAGCGCCTGGTCTTCGCTGGCGGGCGAGTGGTGCTGACCCTGCTGATCGGCGACGTGCTGGAATGT
CTGCCCAGCCTGGACGCACGCATCGATGCATGGTTTCTCGACGGCTTCGCGCCGGCGAAGAATCCGCAGATGTGGCAACC
GGCACTGTTCAGCGAAATGGCCCGGCTATCCGCACCGGGCGCCACCCTAGGCACCTTTACCAGCGCCGGTTTCGTGCGTC
GCGGTCTGATCGAGGCTGGTTTCGACATGCAGCGTGTGCCGGGATACGGCCACAAGCGCGAGATCCTGCGCGGTCACCTG
CAATCGAGTACCGCGCAGATGGCAGACAAGCCCTGGTTCGCCCGGCCAGGCCGTACCGTCCGCGAACGCCGTGCCGTGGT
GATCGGCGCCGGCCTGGCCGGTTGCGCCACGGCTGCCGCGCTGGCCGCCCGTGGCTGGCGGGTCAGCGTTCTGGAGCGCC
ATGCCGACGCCGCGCAGGAGGGCTCCGGCAATCCGCAGGGGGTGCTCTACCTCAAGCTGTCGGCTCACGGCACCGCGCTG
TCGAAGCTGGTGGTCGGTGGCTTCAGCTATACCCGCCGACTGCTCGGCCACCTGCAGCAGGGCCAGGACTGGCAAGCCTG
TGGTGTGCTGCAGCTGATCTACGACGACAAGGAACGCCAACGCCAGGCTGAGCTGGCGGAGGCTTTTCCGCCCTCACTGG
TTCATCCGCTCGAACGCGACGCCGCCGAAGCACTGGCCGGCGTGGCCCTGACCGAAGGTGGCTTGTTCTATCCCGATGCC
GGCTGGGCCCATCCACCCGCGCTGTGCCGCTGGCTGCTGCAGCGACCGGGTATCGAGCTGCTGCGGCATCGGGAGGCCCT
CGATCTGCGCCGCCACGGCGAGGCCTGGCAGGTGCTCGGCGGCGAGGGCGTGCTGGCCGAGGCGCCGGTCGTGGTGCTCG
CTGGCGCAGCCGATGCGGCGCGCTTCGAGCAGAGCGCCTGGCTACCGCTCAAACGCATCCGCGGGCAGATTTCTGCCCTC
CCCGCCACGGCCCGCAGCGGCCAGTTGCGCACCGTGCTGTGCGCCAAGGGCTACGTTGCACCGCCACGAGACGGCACCCA
TACCCTTGGTGCGAGCTTCAATTTCCAGCAGACCGACGACGCCCCCAGTGTCGCCGAACACCAGGCGAACCTGGAGATGC
TCGAGCAGATCTCGACTGACCTCTACCAACGCCTGCAGGCCGATCCGCAGCGCACCGAAGCCCTGCAGGGCCGCGTCGCG
TTTCGGTGCACCAGCCCGGACTACCTGCCGCTGATCGGGCCGCTGGCCGACCCGCAGCGCTTCGCCGAAGCCTACGCGGC
GTTGGCCAGGAACGCGCGGCAGTCCGTGCAGGCCGTCTGCCCCTGGCTGGACGGCCTCTATGTGAACACCGCCCACGGCT
CGCGCGGCATGATCAGCGCGCCGCTCTCCGGCGAGCTGCTTGCCGCCTGGCTGGATGACGAGCCGCTACCATTGCCACGG
GAAGTGGCAGAAGCCTGCCATCCCAACCGCTTCCTGCTACGCAAGCTGATTCGCGGCACCTGA

Upstream 100 bases:

>100_bases
CCGCCTGGAGCAGGAAATCCACGCCAACGGCTGACCGACCGGGCCGGGCATTGCCCGGCCCTTCTCCTTTCGCGCCACCC
CTGACCGATCAGGAACCATG

Downstream 100 bases:

>100_bases
ACGAGCAGACAGCGCAAGCCGTCACCCTGCCGGTGACGGCTTGCATCGAGGCGCGCGCCGAAAGATACTCCCCCCACTAT
ACAAAGCAACCGGCGTAGGT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MNDHPAQDAFQHAELDWDENGLPQSRHYGDVYFSRASGLAETEHVFLAQNDLPRRFAAMQPDECLVIGETGFGTGLNFLC
AWQLFERSASARARLHFVSVEKHPLTFADMQRALALWPELQAQAEQLLEQYVALNPGYQRLVFAGGRVVLTLLIGDVLEC
LPSLDARIDAWFLDGFAPAKNPQMWQPALFSEMARLSAPGATLGTFTSAGFVRRGLIEAGFDMQRVPGYGHKREILRGHL
QSSTAQMADKPWFARPGRTVRERRAVVIGAGLAGCATAAALAARGWRVSVLERHADAAQEGSGNPQGVLYLKLSAHGTAL
SKLVVGGFSYTRRLLGHLQQGQDWQACGVLQLIYDDKERQRQAELAEAFPPSLVHPLERDAAEALAGVALTEGGLFYPDA
GWAHPPALCRWLLQRPGIELLRHREALDLRRHGEAWQVLGGEGVLAEAPVVVLAGAADAARFEQSAWLPLKRIRGQISAL
PATARSGQLRTVLCAKGYVAPPRDGTHTLGASFNFQQTDDAPSVAEHQANLEMLEQISTDLYQRLQADPQRTEALQGRVA
FRCTSPDYLPLIGPLADPQRFAEAYAALARNARQSVQAVCPWLDGLYVNTAHGSRGMISAPLSGELLAAWLDDEPLPLPR
EVAEACHPNRFLLRKLIRGT

Sequences:

>Translated_660_residues
MNDHPAQDAFQHAELDWDENGLPQSRHYGDVYFSRASGLAETEHVFLAQNDLPRRFAAMQPDECLVIGETGFGTGLNFLC
AWQLFERSASARARLHFVSVEKHPLTFADMQRALALWPELQAQAEQLLEQYVALNPGYQRLVFAGGRVVLTLLIGDVLEC
LPSLDARIDAWFLDGFAPAKNPQMWQPALFSEMARLSAPGATLGTFTSAGFVRRGLIEAGFDMQRVPGYGHKREILRGHL
QSSTAQMADKPWFARPGRTVRERRAVVIGAGLAGCATAAALAARGWRVSVLERHADAAQEGSGNPQGVLYLKLSAHGTAL
SKLVVGGFSYTRRLLGHLQQGQDWQACGVLQLIYDDKERQRQAELAEAFPPSLVHPLERDAAEALAGVALTEGGLFYPDA
GWAHPPALCRWLLQRPGIELLRHREALDLRRHGEAWQVLGGEGVLAEAPVVVLAGAADAARFEQSAWLPLKRIRGQISAL
PATARSGQLRTVLCAKGYVAPPRDGTHTLGASFNFQQTDDAPSVAEHQANLEMLEQISTDLYQRLQADPQRTEALQGRVA
FRCTSPDYLPLIGPLADPQRFAEAYAALARNARQSVQAVCPWLDGLYVNTAHGSRGMISAPLSGELLAAWLDDEPLPLPR
EVAEACHPNRFLLRKLIRGT
>Mature_660_residues
MNDHPAQDAFQHAELDWDENGLPQSRHYGDVYFSRASGLAETEHVFLAQNDLPRRFAAMQPDECLVIGETGFGTGLNFLC
AWQLFERSASARARLHFVSVEKHPLTFADMQRALALWPELQAQAEQLLEQYVALNPGYQRLVFAGGRVVLTLLIGDVLEC
LPSLDARIDAWFLDGFAPAKNPQMWQPALFSEMARLSAPGATLGTFTSAGFVRRGLIEAGFDMQRVPGYGHKREILRGHL
QSSTAQMADKPWFARPGRTVRERRAVVIGAGLAGCATAAALAARGWRVSVLERHADAAQEGSGNPQGVLYLKLSAHGTAL
SKLVVGGFSYTRRLLGHLQQGQDWQACGVLQLIYDDKERQRQAELAEAFPPSLVHPLERDAAEALAGVALTEGGLFYPDA
GWAHPPALCRWLLQRPGIELLRHREALDLRRHGEAWQVLGGEGVLAEAPVVVLAGAADAARFEQSAWLPLKRIRGQISAL
PATARSGQLRTVLCAKGYVAPPRDGTHTLGASFNFQQTDDAPSVAEHQANLEMLEQISTDLYQRLQADPQRTEALQGRVA
FRCTSPDYLPLIGPLADPQRFAEAYAALARNARQSVQAVCPWLDGLYVNTAHGSRGMISAPLSGELLAAWLDDEPLPLPR
EVAEACHPNRFLLRKLIRGT

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=40.990990990991, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSEU5 (A4VMX4)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173167.1
- ProteinModelPortal:   A4VMX4
- STRING:   A4VMX4
- GeneID:   5095637
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_2675
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A4VMX4
- ProtClustDB:   PRK01747
- BioCyc:   PSTU379731:PST_2675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 72337; Mature: 72337

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHPAQDAFQHAELDWDENGLPQSRHYGDVYFSRASGLAETEHVFLAQNDLPRRFAAMQ
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCHHHHCCC
PDECLVIGETGFGTGLNFLCAWQLFERSASARARLHFVSVEKHPLTFADMQRALALWPEL
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
QAQAEQLLEQYVALNPGYQRLVFAGGRVVLTLLIGDVLECLPSLDARIDAWFLDGFAPAK
HHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
NPQMWQPALFSEMARLSAPGATLGTFTSAGFVRRGLIEAGFDMQRVPGYGHKREILRGHL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
QSSTAQMADKPWFARPGRTVRERRAVVIGAGLAGCATAAALAARGWRVSVLERHADAAQE
HHHHHHHHCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
GSGNPQGVLYLKLSAHGTALSKLVVGGFSYTRRLLGHLQQGQDWQACGVLQLIYDDKERQ
CCCCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCHHEECCCHHHH
RQAELAEAFPPSLVHPLERDAAEALAGVALTEGGLFYPDAGWAHPPALCRWLLQRPGIEL
HHHHHHHHCCCHHHCHHHHHHHHHHHHHHEECCCEECCCCCCCCCHHHHHHHHHCCCHHH
LRHREALDLRRHGEAWQVLGGEGVLAEAPVVVLAGAADAARFEQSAWLPLKRIRGQISAL
HHHHHHHHHHHCCCCEEEECCCCCEECCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHC
PATARSGQLRTVLCAKGYVAPPRDGTHTLGASFNFQQTDDAPSVAEHQANLEMLEQISTD
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LYQRLQADPQRTEALQGRVAFRCTSPDYLPLIGPLADPQRFAEAYAALARNARQSVQAVC
HHHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PWLDGLYVNTAHGSRGMISAPLSGELLAAWLDDEPLPLPREVAEACHPNRFLLRKLIRGT
HHHCCEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNDHPAQDAFQHAELDWDENGLPQSRHYGDVYFSRASGLAETEHVFLAQNDLPRRFAAMQ
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCHHHHCCC
PDECLVIGETGFGTGLNFLCAWQLFERSASARARLHFVSVEKHPLTFADMQRALALWPEL
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
QAQAEQLLEQYVALNPGYQRLVFAGGRVVLTLLIGDVLECLPSLDARIDAWFLDGFAPAK
HHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
NPQMWQPALFSEMARLSAPGATLGTFTSAGFVRRGLIEAGFDMQRVPGYGHKREILRGHL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
QSSTAQMADKPWFARPGRTVRERRAVVIGAGLAGCATAAALAARGWRVSVLERHADAAQE
HHHHHHHHCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
GSGNPQGVLYLKLSAHGTALSKLVVGGFSYTRRLLGHLQQGQDWQACGVLQLIYDDKERQ
CCCCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCHHEECCCHHHH
RQAELAEAFPPSLVHPLERDAAEALAGVALTEGGLFYPDAGWAHPPALCRWLLQRPGIEL
HHHHHHHHCCCHHHCHHHHHHHHHHHHHHEECCCEECCCCCCCCCHHHHHHHHHCCCHHH
LRHREALDLRRHGEAWQVLGGEGVLAEAPVVVLAGAADAARFEQSAWLPLKRIRGQISAL
HHHHHHHHHHHCCCCEEEECCCCCEECCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHC
PATARSGQLRTVLCAKGYVAPPRDGTHTLGASFNFQQTDDAPSVAEHQANLEMLEQISTD
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LYQRLQADPQRTEALQGRVAFRCTSPDYLPLIGPLADPQRFAEAYAALARNARQSVQAVC
HHHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PWLDGLYVNTAHGSRGMISAPLSGELLAAWLDDEPLPLPREVAEACHPNRFLLRKLIRGT
HHHCCEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA