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The map label for this gene is entD [H]

Identifier: 146282845

GI number: 146282845

Start: 2733582

End: 2734310

Strand: Direct

Name: entD [H]

Synonym: PST_2504

Alternate gene names: 146282845

Gene position: 2733582-2734310 (Clockwise)

Preceding gene: 146282842

Following gene: 146282846

Centisome position: 59.85

GC content: 65.84

Gene sequence:

>729_bases
ATGATCGAGCGCCCGCCCTACCCACACTGCTGCACGCCGCCCCAGGACCATTGGCCCTTGCCCGAGGCATTGCCCGGTGT
TCGACTATTGAGCACGCAGTTCGACTCCGCCTTGCTCGACCCCGAAGACTTCACCCGCTGGGGCATTGCCGCGCCGCGAG
GCGTTAGCAAGCGACAGGCAGAGTTCCTGGCCGGCCGCCTATGTGCACGGGAAGCGTTACAGAGCCTGACCGGCACACCC
TATGTGCCAACCGTGGGCGAGGACCGCGCCCCGCAATGGCCGCGCGGCGTCGTTGGCTCCATCACCCATGGCGCCGGTTG
GGCAGGCGTCGTGGTAGCGCGGCAACGCGAATGGTGCGGTCTGGGGCTGGATATCGAACGCATGCTGGTGTGCGAGCGTG
CCGACCGCCTGGCGGGTGAGATTCTCACGCCCAGCGAACTGGAGGCGTTTGCATCGCTGGGCGACCACGAACGCGCCGAG
TTGGTCACCCGCACCTTCTCGCTCAAGGAAAGCCTGTTCAAGGCCCTCTACCCGTTGGTCAAGCAACGCTTCTACTTTCA
GGATGCCGAGGTGCTGGAGGCCACACCTGGGGGGCTCGCGCGCCTGCGCCTGCTTATCGACCTTTCGCAGACCTGGCGCA
CCGGCGCATTGGTCGAAGGCCAGTTCGCCCGTTTCGACGGCTACCTGCTGAGCCTGGTCAGTATCCCCACCGCGCCTTCG
CCAGATTGA

Upstream 100 bases:

>100_bases
TGCCAAGTCACCGCACGCCCCGCTGCCGGCCGGCTCCATGAGCCGCTGCAGCGCGCGTTCCGTTGAACGATCGCAGCGCA
CTTCAGGCAGAATACCGGCC

Downstream 100 bases:

>100_bases
AGAGACGGAGGCCGACAGACGTCGAAGCGCCCCGCCTGGCTGGTCCATCATGCTGATTCGATAGGCTGGAACCGAACCAT
CAGGCGCCAGACGAGTCAAC

Product: siderophore biosynthesis protein, putative

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIERPPYPHCCTPPQDHWPLPEALPGVRLLSTQFDSALLDPEDFTRWGIAAPRGVSKRQAEFLAGRLCAREALQSLTGTP
YVPTVGEDRAPQWPRGVVGSITHGAGWAGVVVARQREWCGLGLDIERMLVCERADRLAGEILTPSELEAFASLGDHERAE
LVTRTFSLKESLFKALYPLVKQRFYFQDAEVLEATPGGLARLRLLIDLSQTWRTGALVEGQFARFDGYLLSLVSIPTAPS
PD

Sequences:

>Translated_242_residues
MIERPPYPHCCTPPQDHWPLPEALPGVRLLSTQFDSALLDPEDFTRWGIAAPRGVSKRQAEFLAGRLCAREALQSLTGTP
YVPTVGEDRAPQWPRGVVGSITHGAGWAGVVVARQREWCGLGLDIERMLVCERADRLAGEILTPSELEAFASLGDHERAE
LVTRTFSLKESLFKALYPLVKQRFYFQDAEVLEATPGGLARLRLLIDLSQTWRTGALVEGQFARFDGYLLSLVSIPTAPS
PD
>Mature_242_residues
MIERPPYPHCCTPPQDHWPLPEALPGVRLLSTQFDSALLDPEDFTRWGIAAPRGVSKRQAEFLAGRLCAREALQSLTGTP
YVPTVGEDRAPQWPRGVVGSITHGAGWAGVVVARQREWCGLGLDIERMLVCERADRLAGEILTPSELEAFASLGDHERAE
LVTRTFSLKESLFKALYPLVKQRFYFQDAEVLEATPGGLARLRLLIDLSQTWRTGALVEGQFARFDGYLLSLVSIPTAPS
PD

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=119, Percent_Identity=43.6974789915966, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 26766; Mature: 26766

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERPPYPHCCTPPQDHWPLPEALPGVRLLSTQFDSALLDPEDFTRWGIAAPRGVSKRQA
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHH
EFLAGRLCAREALQSLTGTPYVPTVGEDRAPQWPRGVVGSITHGAGWAGVVVARQREWCG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCC
LGLDIERMLVCERADRLAGEILTPSELEAFASLGDHERAELVTRTFSLKESLFKALYPLV
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQRFYFQDAEVLEATPGGLARLRLLIDLSQTWRTGALVEGQFARFDGYLLSLVSIPTAPS
HHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCC
PD
CC
>Mature Secondary Structure
MIERPPYPHCCTPPQDHWPLPEALPGVRLLSTQFDSALLDPEDFTRWGIAAPRGVSKRQA
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHH
EFLAGRLCAREALQSLTGTPYVPTVGEDRAPQWPRGVVGSITHGAGWAGVVVARQREWCG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCC
LGLDIERMLVCERADRLAGEILTPSELEAFASLGDHERAELVTRTFSLKESLFKALYPLV
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQRFYFQDAEVLEATPGGLARLRLLIDLSQTWRTGALVEGQFARFDGYLLSLVSIPTAPS
HHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCC
PD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]