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The map label for this gene is slt [H]

Identifier: 146282827

GI number: 146282827

Start: 2711229

End: 2713211

Strand: Direct

Name: slt [H]

Synonym: PST_2486

Alternate gene names: 146282827

Gene position: 2711229-2713211 (Clockwise)

Preceding gene: 146282824

Following gene: 146282831

Centisome position: 59.36

GC content: 64.04

Gene sequence:

>1983_bases
GTGCCGCCCGGTACAGCACGTCGCCTGTCATCTCAACGCCCCCCGTTTTACGGATGTACCATGCGCGGCCGACTCTCCAG
TTTGTGTTTCTGCCTGTTCGTTTCTGCCATTGGCCTGGATGCGTCCGCCGCCAGTCTCAGCCAGCAACGCCAGTATTACG
ATGAGGCCAAGCAGGCCCTGGCCAAGGGCGATGCAGGCCCCTATCGCCGTTACCAGAGCGCCTTGCGCGACTATCCGCTC
GAGCCCTACCTCGCCTATGACGAGCTGACCCACCGGCTGAAGTCGGCCAGCAATCAGGAAGTCGAGAAGTTTCTCGCCGA
ACACGGCGACCTGCCTCAGATCAACTGGATGAAGCTGCGCTGGCTGCGCCTGCTGGCCGCCCGCGGCGACTGGCAGACCT
TTACCCGCTATTACGATCCGAAGATGAACTTCACCGAGCTGGACTGCCTGTTCGCGCAGTATCAGCTGCGCAACGGTCTC
CCGGGCGCTGACGCCGCAACCGAGCGGCTGTGGCTGGTCGGCAAGTCGCAGCACAACTCCTGCGACCCGCTGTTCGAGCT
CTGGGAATCCAAGGGTCTCCTGACCGAAGACCGGCGCTGGAAGCGCACCAAGCTGGCCGTGGAGAGCGGCAATTACGGGG
TCGCCAGCTTCCTGGTGAAACGTCTGCCTACGCTGCAGAGTCAGGGTCAGCTGATGATCGACGTGGCGCAGAAACCGCAG
ATGCTTCGCCAGTCTGAACGCTTCTCGCCGACCAGCCCGGCGATGGCCGACGTCGTTTCGATCGGCCTGCGGCGCCTCGC
GCGCCAGGACCCGGAGCAGGCGCTCAATCTGCTCGACGGCTACGCCCGCCGCTTTGCGTTTTCGCCCGAGGAAAAGGTCG
CCATTGCCCGGCAGATCGGGCTGACCCTGGCCAAGCGCTTCGACAGCCGCGCGCTCAAGGTGATGGCCGAGTACGATCCC
GAGCTGCGCGACAACACCGTCACCGAGTGGCGCACCCGTCTGCTGCTGCGCCAGGGCCAGTGGGATGAAGCGTACCGGCT
GACACAGCGTTTTCCCGATGAACTGGCCAATACCAATCGCTGGCGCTACTGGCAGGCGCGCAGCCTGCAGCTGGCCAAAC
CGGAGAGCAAGCAGGCGGCCCTGCTCTATAAGCCGGTGGCCGAAGAGCGGGATTTCTACGGCTTCCTGTCGGCCGACCGC
ATCCAGGCGCCGTACAAGCTGAACCATCAGCCACTGGCGCTCGATCCGAAGGTCGTGCAGAAGGTGCGCAATACCGCCGG
TATTCGCCGCGCACTGGAATTCCACGACCGCGGGCAGATCGTCGATGGCCGCCGCGAGTGGTATCACGTCAGTCGGCTGT
TCAGCCGCGACGAGATGGTCGCGCAGGCACGCATCGCCTATGAAAAGGAGTGGTACTTCCCGGCCATCCGCACCATCAGC
CAGGCGCAGTACTGGGATGATCTGGATATCCGCTTCCCCATGGCCCACCGCAACACCCTGGTACAGGCCGCGCGGGCGCG
GGAGATCCATCCCAGCTGGGCGTTCGCCGTCACCCGCCAGGAAAGCGCCTTCATGGCCGATGCCCGCTCCCATGTCGGCG
CCACCGGCCTGATGCAGCTGATGCCCGCAACCGCCAAGGAAACCGCCAAGCGCTTCAACATCCCGCTGGCTTCGCCGCAG
CAGGTCCTGAACCCCACCACCAACATCCAGCTGGGCACTGCCTATCTCAGCCAGATCTACGGGCAGTTCAACGGCAACCG
TGTACTGGCCTCGGCCGCCTACAACGCCGGCCCCGGTCGTGTGCGCCAGTGGCTGCGCAACGCCGAGCACCTGTCGTTCG
ATGTGTGGGTGGAAAACATTCCGTTCGACGAGACCCGCCAGTACGTGCAGAACGTGCTGACCTACTCGGTGATCTACGGG
CAGAAGCTGAATTCACCCCAACCGCTGGTGGAGTGGCACGAGCGTTATTTCGAATCGCAGTGA

Upstream 100 bases:

>100_bases
CGCTCGTCGGAAGAGTGGTTTTGTTTGTTACCGGAGCTTAATGCTCGTTTAACTGGCCGGTGCGGCAAGCTAAGCTAGCT
TCTGCAGCAGGCCGTCTCCT

Downstream 100 bases:

>100_bases
CGCGCAGCCGGCCCGGACGGCACACCGTCCGGGCGCGGCCGTTTCTCACTCCAGCCGCTCGACCGGCATTCCGACTTCCA
GCTCGCCGCGCCCTTCGGCG

Product: soluble lytic transglycosylase, putative

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MPPGTARRLSSQRPPFYGCTMRGRLSSLCFCLFVSAIGLDASAASLSQQRQYYDEAKQALAKGDAGPYRRYQSALRDYPL
EPYLAYDELTHRLKSASNQEVEKFLAEHGDLPQINWMKLRWLRLLAARGDWQTFTRYYDPKMNFTELDCLFAQYQLRNGL
PGADAATERLWLVGKSQHNSCDPLFELWESKGLLTEDRRWKRTKLAVESGNYGVASFLVKRLPTLQSQGQLMIDVAQKPQ
MLRQSERFSPTSPAMADVVSIGLRRLARQDPEQALNLLDGYARRFAFSPEEKVAIARQIGLTLAKRFDSRALKVMAEYDP
ELRDNTVTEWRTRLLLRQGQWDEAYRLTQRFPDELANTNRWRYWQARSLQLAKPESKQAALLYKPVAEERDFYGFLSADR
IQAPYKLNHQPLALDPKVVQKVRNTAGIRRALEFHDRGQIVDGRREWYHVSRLFSRDEMVAQARIAYEKEWYFPAIRTIS
QAQYWDDLDIRFPMAHRNTLVQAARAREIHPSWAFAVTRQESAFMADARSHVGATGLMQLMPATAKETAKRFNIPLASPQ
QVLNPTTNIQLGTAYLSQIYGQFNGNRVLASAAYNAGPGRVRQWLRNAEHLSFDVWVENIPFDETRQYVQNVLTYSVIYG
QKLNSPQPLVEWHERYFESQ

Sequences:

>Translated_660_residues
MPPGTARRLSSQRPPFYGCTMRGRLSSLCFCLFVSAIGLDASAASLSQQRQYYDEAKQALAKGDAGPYRRYQSALRDYPL
EPYLAYDELTHRLKSASNQEVEKFLAEHGDLPQINWMKLRWLRLLAARGDWQTFTRYYDPKMNFTELDCLFAQYQLRNGL
PGADAATERLWLVGKSQHNSCDPLFELWESKGLLTEDRRWKRTKLAVESGNYGVASFLVKRLPTLQSQGQLMIDVAQKPQ
MLRQSERFSPTSPAMADVVSIGLRRLARQDPEQALNLLDGYARRFAFSPEEKVAIARQIGLTLAKRFDSRALKVMAEYDP
ELRDNTVTEWRTRLLLRQGQWDEAYRLTQRFPDELANTNRWRYWQARSLQLAKPESKQAALLYKPVAEERDFYGFLSADR
IQAPYKLNHQPLALDPKVVQKVRNTAGIRRALEFHDRGQIVDGRREWYHVSRLFSRDEMVAQARIAYEKEWYFPAIRTIS
QAQYWDDLDIRFPMAHRNTLVQAARAREIHPSWAFAVTRQESAFMADARSHVGATGLMQLMPATAKETAKRFNIPLASPQ
QVLNPTTNIQLGTAYLSQIYGQFNGNRVLASAAYNAGPGRVRQWLRNAEHLSFDVWVENIPFDETRQYVQNVLTYSVIYG
QKLNSPQPLVEWHERYFESQ
>Mature_659_residues
PPGTARRLSSQRPPFYGCTMRGRLSSLCFCLFVSAIGLDASAASLSQQRQYYDEAKQALAKGDAGPYRRYQSALRDYPLE
PYLAYDELTHRLKSASNQEVEKFLAEHGDLPQINWMKLRWLRLLAARGDWQTFTRYYDPKMNFTELDCLFAQYQLRNGLP
GADAATERLWLVGKSQHNSCDPLFELWESKGLLTEDRRWKRTKLAVESGNYGVASFLVKRLPTLQSQGQLMIDVAQKPQM
LRQSERFSPTSPAMADVVSIGLRRLARQDPEQALNLLDGYARRFAFSPEEKVAIARQIGLTLAKRFDSRALKVMAEYDPE
LRDNTVTEWRTRLLLRQGQWDEAYRLTQRFPDELANTNRWRYWQARSLQLAKPESKQAALLYKPVAEERDFYGFLSADRI
QAPYKLNHQPLALDPKVVQKVRNTAGIRRALEFHDRGQIVDGRREWYHVSRLFSRDEMVAQARIAYEKEWYFPAIRTISQ
AQYWDDLDIRFPMAHRNTLVQAARAREIHPSWAFAVTRQESAFMADARSHVGATGLMQLMPATAKETAKRFNIPLASPQQ
VLNPTTNIQLGTAYLSQIYGQFNGNRVLASAAYNAGPGRVRQWLRNAEHLSFDVWVENIPFDETRQYVQNVLTYSVIYGQ
KLNSPQPLVEWHERYFESQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=646, Percent_Identity=32.5077399380805, Blast_Score=298, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76304; Mature: 76173

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPGTARRLSSQRPPFYGCTMRGRLSSLCFCLFVSAIGLDASAASLSQQRQYYDEAKQAL
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AKGDAGPYRRYQSALRDYPLEPYLAYDELTHRLKSASNQEVEKFLAEHGDLPQINWMKLR
HCCCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
WLRLLAARGDWQTFTRYYDPKMNFTELDCLFAQYQLRNGLPGADAATERLWLVGKSQHNS
HHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
CDPLFELWESKGLLTEDRRWKRTKLAVESGNYGVASFLVKRLPTLQSQGQLMIDVAQKPQ
CCHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEHHCCH
MLRQSERFSPTSPAMADVVSIGLRRLARQDPEQALNLLDGYARRFAFSPEEKVAIARQIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTLAKRFDSRALKVMAEYDPELRDNTVTEWRTRLLLRQGQWDEAYRLTQRFPDELANTNR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCC
WRYWQARSLQLAKPESKQAALLYKPVAEERDFYGFLSADRIQAPYKLNHQPLALDPKVVQ
EEEEECCCCEECCCCCCCCEEEECCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHH
KVRNTAGIRRALEFHDRGQIVDGRREWYHVSRLFSRDEMVAQARIAYEKEWYFPAIRTIS
HHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QAQYWDDLDIRFPMAHRNTLVQAARAREIHPSWAFAVTRQESAFMADARSHVGATGLMQL
HHHCCCCCCEEECHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHH
MPATAKETAKRFNIPLASPQQVLNPTTNIQLGTAYLSQIYGQFNGNRVLASAAYNAGPGR
HCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCHHH
VRQWLRNAEHLSFDVWVENIPFDETRQYVQNVLTYSVIYGQKLNSPQPLVEWHERYFESQ
HHHHHHCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PPGTARRLSSQRPPFYGCTMRGRLSSLCFCLFVSAIGLDASAASLSQQRQYYDEAKQAL
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AKGDAGPYRRYQSALRDYPLEPYLAYDELTHRLKSASNQEVEKFLAEHGDLPQINWMKLR
HCCCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
WLRLLAARGDWQTFTRYYDPKMNFTELDCLFAQYQLRNGLPGADAATERLWLVGKSQHNS
HHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
CDPLFELWESKGLLTEDRRWKRTKLAVESGNYGVASFLVKRLPTLQSQGQLMIDVAQKPQ
CCHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEHHCCH
MLRQSERFSPTSPAMADVVSIGLRRLARQDPEQALNLLDGYARRFAFSPEEKVAIARQIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTLAKRFDSRALKVMAEYDPELRDNTVTEWRTRLLLRQGQWDEAYRLTQRFPDELANTNR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCC
WRYWQARSLQLAKPESKQAALLYKPVAEERDFYGFLSADRIQAPYKLNHQPLALDPKVVQ
EEEEECCCCEECCCCCCCCEEEECCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHH
KVRNTAGIRRALEFHDRGQIVDGRREWYHVSRLFSRDEMVAQARIAYEKEWYFPAIRTIS
HHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QAQYWDDLDIRFPMAHRNTLVQAARAREIHPSWAFAVTRQESAFMADARSHVGATGLMQL
HHHCCCCCCEEECHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHH
MPATAKETAKRFNIPLASPQQVLNPTTNIQLGTAYLSQIYGQFNGNRVLASAAYNAGPGR
HCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCHHH
VRQWLRNAEHLSFDVWVENIPFDETRQYVQNVLTYSVIYGQKLNSPQPLVEWHERYFESQ
HHHHHHCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]