Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 146282753

GI number: 146282753

Start: 2637117

End: 2637956

Strand: Direct

Name: cobA [H]

Synonym: PST_2412

Alternate gene names: 146282753

Gene position: 2637117-2637956 (Clockwise)

Preceding gene: 146282752

Following gene: 146282762

Centisome position: 57.74

GC content: 67.38

Gene sequence:

>840_bases
GTGGCACCAGCTGCGGCTCCTGCGTGCCCGAACTCAAGCGCCTGCTGGCCCACCAGCCGGAACGCGCACTTCTCTGATAC
CAACCAAGACGAGGATTACCCCATGAGCGCAAAAGTCTGGCTGGTAGGCGCCGGCCCTGGCGACCCGGAGCTGCTGACCC
TCAAGGCCGTACGCGCACTGGGCGAGGCACAGGTGGTGATGATCGATGACCTGGTCAACCCCGCCGTGCTCGAACATTGC
CCGAACGCTCGCATCATCCCGGTGGGCAAGCGCGGCGGCTGCCGCTCGACGCCGCAGGCCTTCATCCACCGGCTGATGCT
GCGCTACGCCCGTCAGGGCAAGTGTGTGGTGCGCCTCAAGGGTGGCGATCCTTGCATCTTCGGCCGTGGCGGCGAGGAGG
CCGAATGGCTGGCAGCGCGCGGCATCGAGGTCGAGCTGGTCAACGGCATCACCGCCGGCCTGGCCGGCGCCACGCAATGC
GGCATCTCGCTGACCCAGCGCGGCGTAGCGCGCGGCGTAACACTGGTCACCGCACACACCCAGGATGACAGCAGCCTCGA
ATGGCAAGCGCTGGCCAAAAGCGGCACCACGCTAGTGGTGTACATGGGTGTAAGCAAGCTGGAGCAGGTGCAGGCCGGGC
TGCTGGAAGGCGGGATGAACGCCAGCACGCCGGTGGCCATGATCGAGAACGCCTCGCTGCCCCAGCAGCGCGAATGCCGC
TCCAGTCTCGCCGGCATGTGTCGCGACGCCCAGGCCTTCGAGCTGAAGAGCCCGGCCATCCTGGTGATCGGTGACGTGGC
CGCGCAGGCCGTTGCGCAAATGCTGAGCCAGACGGCCTGA

Upstream 100 bases:

>100_bases
GACCGCACGCTGTGCAATTGCCTGAACGTCAGCCAGAGCGCGATCTGCGCTGGCATCGAGCGCGGTCTCGATCTGACCGG
CCTCAAGCAGGAACTCGGCT

Downstream 100 bases:

>100_bases
TCCTCCGGGGCCCGGAGCGTTGGCCCCCCTTCCCTTTACTTCCACTCAAAAAAAACCGCGGCCCAAGGGCCGCGGTTCTT
CGCTACATCTCGGCTCACCC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAPAAAPACPNSSACWPTSRNAHFSDTNQDEDYPMSAKVWLVGAGPGDPELLTLKAVRALGEAQVVMIDDLVNPAVLEHC
PNARIIPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLKGGDPCIFGRGGEEAEWLAARGIEVELVNGITAGLAGATQC
GISLTQRGVARGVTLVTAHTQDDSSLEWQALAKSGTTLVVYMGVSKLEQVQAGLLEGGMNASTPVAMIENASLPQQRECR
SSLAGMCRDAQAFELKSPAILVIGDVAAQAVAQMLSQTA

Sequences:

>Translated_279_residues
MAPAAAPACPNSSACWPTSRNAHFSDTNQDEDYPMSAKVWLVGAGPGDPELLTLKAVRALGEAQVVMIDDLVNPAVLEHC
PNARIIPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLKGGDPCIFGRGGEEAEWLAARGIEVELVNGITAGLAGATQC
GISLTQRGVARGVTLVTAHTQDDSSLEWQALAKSGTTLVVYMGVSKLEQVQAGLLEGGMNASTPVAMIENASLPQQRECR
SSLAGMCRDAQAFELKSPAILVIGDVAAQAVAQMLSQTA
>Mature_278_residues
APAAAPACPNSSACWPTSRNAHFSDTNQDEDYPMSAKVWLVGAGPGDPELLTLKAVRALGEAQVVMIDDLVNPAVLEHCP
NARIIPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLKGGDPCIFGRGGEEAEWLAARGIEVELVNGITAGLAGATQCG
ISLTQRGVARGVTLVTAHTQDDSSLEWQALAKSGTTLVVYMGVSKLEQVQAGLLEGGMNASTPVAMIENASLPQQRECRS
SLAGMCRDAQAFELKSPAILVIGDVAAQAVAQMLSQTA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=42.436974789916, Blast_Score=173, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322922, Length=259, Percent_Identity=29.3436293436293, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 29321; Mature: 29190

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAAAPACPNSSACWPTSRNAHFSDTNQDEDYPMSAKVWLVGAGPGDPELLTLKAVRAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEHHHHHHHC
GEAQVVMIDDLVNPAVLEHCPNARIIPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLK
CCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
GGDPCIFGRGGEEAEWLAARGIEVELVNGITAGLAGATQCGISLTQRGVARGVTLVTAHT
CCCEEEECCCCCCHHHHHHCCCEEEEECCHHHHCCCHHHHCHHHHHHHHHCCEEEEEEEC
QDDSSLEWQALAKSGTTLVVYMGVSKLEQVQAGLLEGGMNASTPVAMIENASLPQQRECR
CCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
SSLAGMCRDAQAFELKSPAILVIGDVAAQAVAQMLSQTA
HHHHHHHCCCCCEEECCCEEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APAAAPACPNSSACWPTSRNAHFSDTNQDEDYPMSAKVWLVGAGPGDPELLTLKAVRAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEHHHHHHHC
GEAQVVMIDDLVNPAVLEHCPNARIIPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLK
CCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
GGDPCIFGRGGEEAEWLAARGIEVELVNGITAGLAGATQCGISLTQRGVARGVTLVTAHT
CCCEEEECCCCCCHHHHHHCCCEEEEECCHHHHCCCHHHHCHHHHHHHHHCCEEEEEEEC
QDDSSLEWQALAKSGTTLVVYMGVSKLEQVQAGLLEGGMNASTPVAMIENASLPQQRECR
CCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
SSLAGMCRDAQAFELKSPAILVIGDVAAQAVAQMLSQTA
HHHHHHHCCCCCEEECCCEEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]