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The map label for this gene is puuB [H]

Identifier: 146282713

GI number: 146282713

Start: 2592176

End: 2593459

Strand: Direct

Name: puuB [H]

Synonym: PST_2371

Alternate gene names: 146282713

Gene position: 2592176-2593459 (Clockwise)

Preceding gene: 146282704

Following gene: 146282716

Centisome position: 56.75

GC content: 59.5

Gene sequence:

>1284_bases
ATGCCGCACAAGGACTATCCAGCTTCCTACTATGCTGCAACCTCCAATCAAACCGTATCTCGCCCCGCGCTAGCTGATCA
TCATCAGGTCGACGTGTGCGTCATTGGCGCCGGTTACACCGGTCTGTCCACTGCCCTGTTCCTGCTCGAGAAGGGCTTCA
GCGTCACCGTTCTGGAAGCCGTGAAGGTTGGCTTCGGCGCATCCGGTCGCAACGGCGGGCAGATCGTCAACAGCTACAGC
CGAGACCTCGACAGCATTGAGCGCAGCGTCAGCGAGCCTGCCGCCAAGCTGATCGGCGAAATGGCATTTGAAGGCGGAAG
GATCATCCGCGATCGGGTCGCCCGCTACAACATCAGGTGCGACCTGAAAGACGGCGGTGTGTTTGCTGCTTTCAACAAGA
AGCAGATGCACCATCTCGAAGCGCAGAAGGCGCTATGGGAGCGATACGGCTACCAGCATCTCGAATTGCTGGACCAGCAT
GATATCCAGCAGGTTGTAGCGTCTGATCGCTACGCCGGCGGCATGCTCGATAAACAAGGCGGCCATATCCACCCGCTCAA
TCTGGCCTTGGGAGAAGCTACGGCGATCGAGTCTCTCGGCGGCATCATCTATGAGAACTCGCCAGCGATCCGCGTCGAGC
GCGGCGAGCAGCCGGTAGTGCATACGCCTGATGGGAGTATTCGCGCTAGGTTCGTGGTGGTGGCAGGCAATGCGTACCTA
GGCGGCCTGATCCCTGAACTGGCGGCAAAATCGATGCCTTGCGGAACCCAGGTGATAGCTACCGAGCCTCTTGACGAGGA
ACTCGCTCGCAGCCTTCTGCCCCAGGATTACTGCGTCGAGGACTGCAACTATCTGCTCGACTACTATCGACTCTCGGCAG
ACAAGAGCCTCATCTACGGAGGTGGGGTCGTTTATGGTGCGCGAGATCCAGCTGATGTCACCTCGATCATCCGACCGAAG
ATGCTGAAAACATTCCCACAACTGGCGAATGTGAAAGTCGACTACGCCTGGACGGGCAACTTCCTGCTCACGCTGTCGCG
CCTGCCACAAGTCGGGCGGCTGGGAAGCAACATCTATTACTCTCAAGGCTGCAGCGGTCACGGCGTCACCTACACCCACG
TCGCCGGCAAAGTGATCGCCGAAGCACTGAGCGGCCAGGCCGAGCGTTTCGATGCTTTCGCCAGCCTGCCGCACTACCCG
TTCCCAGGCGGTCAGCGCTTCCAGGCGCCGCTCAGCGCTCTCGGCGCCATGTACTACAGTCTGCGCGACCGAATCGGGTT
CTAA

Upstream 100 bases:

>100_bases
TTTGGAAAATTTGGAAATCCACACGTATCGCTGGGCCTACCTGGCAAGGCGGCCGGTTAAGCGCTAGCATGCGTAAAATA
TTTAGAACAGAGATTGCAAT

Downstream 100 bases:

>100_bases
TGAACAGGCAGCGGGCACGACGGTGGCGGAGCGCATTGCCGAGCCGCCTGCTCTGCGGCAGCCGGCAAAGGCGCCTTTAG
ACGGCCATCACCCGGTGCAA

Product: glycine/D-amino acid oxidase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPHKDYPASYYAATSNQTVSRPALADHHQVDVCVIGAGYTGLSTALFLLEKGFSVTVLEAVKVGFGASGRNGGQIVNSYS
RDLDSIERSVSEPAAKLIGEMAFEGGRIIRDRVARYNIRCDLKDGGVFAAFNKKQMHHLEAQKALWERYGYQHLELLDQH
DIQQVVASDRYAGGMLDKQGGHIHPLNLALGEATAIESLGGIIYENSPAIRVERGEQPVVHTPDGSIRARFVVVAGNAYL
GGLIPELAAKSMPCGTQVIATEPLDEELARSLLPQDYCVEDCNYLLDYYRLSADKSLIYGGGVVYGARDPADVTSIIRPK
MLKTFPQLANVKVDYAWTGNFLLTLSRLPQVGRLGSNIYYSQGCSGHGVTYTHVAGKVIAEALSGQAERFDAFASLPHYP
FPGGQRFQAPLSALGAMYYSLRDRIGF

Sequences:

>Translated_427_residues
MPHKDYPASYYAATSNQTVSRPALADHHQVDVCVIGAGYTGLSTALFLLEKGFSVTVLEAVKVGFGASGRNGGQIVNSYS
RDLDSIERSVSEPAAKLIGEMAFEGGRIIRDRVARYNIRCDLKDGGVFAAFNKKQMHHLEAQKALWERYGYQHLELLDQH
DIQQVVASDRYAGGMLDKQGGHIHPLNLALGEATAIESLGGIIYENSPAIRVERGEQPVVHTPDGSIRARFVVVAGNAYL
GGLIPELAAKSMPCGTQVIATEPLDEELARSLLPQDYCVEDCNYLLDYYRLSADKSLIYGGGVVYGARDPADVTSIIRPK
MLKTFPQLANVKVDYAWTGNFLLTLSRLPQVGRLGSNIYYSQGCSGHGVTYTHVAGKVIAEALSGQAERFDAFASLPHYP
FPGGQRFQAPLSALGAMYYSLRDRIGF
>Mature_426_residues
PHKDYPASYYAATSNQTVSRPALADHHQVDVCVIGAGYTGLSTALFLLEKGFSVTVLEAVKVGFGASGRNGGQIVNSYSR
DLDSIERSVSEPAAKLIGEMAFEGGRIIRDRVARYNIRCDLKDGGVFAAFNKKQMHHLEAQKALWERYGYQHLELLDQHD
IQQVVASDRYAGGMLDKQGGHIHPLNLALGEATAIESLGGIIYENSPAIRVERGEQPVVHTPDGSIRARFVVVAGNAYLG
GLIPELAAKSMPCGTQVIATEPLDEELARSLLPQDYCVEDCNYLLDYYRLSADKSLIYGGGVVYGARDPADVTSIIRPKM
LKTFPQLANVKVDYAWTGNFLLTLSRLPQVGRLGSNIYYSQGCSGHGVTYTHVAGKVIAEALSGQAERFDAFASLPHYPF
PGGQRFQAPLSALGAMYYSLRDRIGF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=62.8841607565012, Blast_Score=559, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46527; Mature: 46396

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHKDYPASYYAATSNQTVSRPALADHHQVDVCVIGAGYTGLSTALFLLEKGFSVTVLEA
CCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEHH
VKVGFGASGRNGGQIVNSYSRDLDSIERSVSEPAAKLIGEMAFEGGRIIRDRVARYNIRC
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
DLKDGGVFAAFNKKQMHHLEAQKALWERYGYQHLELLDQHDIQQVVASDRYAGGMLDKQG
EECCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GHIHPLNLALGEATAIESLGGIIYENSPAIRVERGEQPVVHTPDGSIRARFVVVAGNAYL
CEEEEEEEECCCHHHHHHHCCEEEECCCEEEEECCCCCEEECCCCCCEEEEEEEECCHHH
GGLIPELAAKSMPCGTQVIATEPLDEELARSLLPQDYCVEDCNYLLDYYRLSADKSLIYG
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GGVVYGARDPADVTSIIRPKMLKTFPQLANVKVDYAWTGNFLLTLSRLPQVGRLGSNIYY
CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHCCCHHHCCCCEEE
SQGCSGHGVTYTHVAGKVIAEALSGQAERFDAFASLPHYPFPGGQRFQAPLSALGAMYYS
ECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LRDRIGF
HHHHCCC
>Mature Secondary Structure 
PHKDYPASYYAATSNQTVSRPALADHHQVDVCVIGAGYTGLSTALFLLEKGFSVTVLEA
CCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEHH
VKVGFGASGRNGGQIVNSYSRDLDSIERSVSEPAAKLIGEMAFEGGRIIRDRVARYNIRC
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
DLKDGGVFAAFNKKQMHHLEAQKALWERYGYQHLELLDQHDIQQVVASDRYAGGMLDKQG
EECCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GHIHPLNLALGEATAIESLGGIIYENSPAIRVERGEQPVVHTPDGSIRARFVVVAGNAYL
CEEEEEEEECCCHHHHHHHCCEEEECCCEEEEECCCCCEEECCCCCCEEEEEEEECCHHH
GGLIPELAAKSMPCGTQVIATEPLDEELARSLLPQDYCVEDCNYLLDYYRLSADKSLIYG
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GGVVYGARDPADVTSIIRPKMLKTFPQLANVKVDYAWTGNFLLTLSRLPQVGRLGSNIYY
CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHCCCHHHCCCCEEE
SQGCSGHGVTYTHVAGKVIAEALSGQAERFDAFASLPHYPFPGGQRFQAPLSALGAMYYS
ECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LRDRIGF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]