| Definition | Pseudomonas stutzeri A1501, complete genome. |
|---|---|
| Accession | NC_009434 |
| Length | 4,567,418 |
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The map label for this gene is purB [H]
Identifier: 146282654
GI number: 146282654
Start: 2517321
End: 2518718
Strand: Direct
Name: purB [H]
Synonym: PST_2306
Alternate gene names: 146282654
Gene position: 2517321-2518718 (Clockwise)
Preceding gene: 146282653
Following gene: 146282655
Centisome position: 55.11
GC content: 63.16
Gene sequence:
>1398_bases TTGCCCGACTGTCCGAGAATGCCCTCTATGCAGCTTTCCTCGCTCACGGCGGTTTCCCCCGTCGATGGCCGCTACGCCGG CAAAACCAGCTCCCTGCGCCCGATCTTCAGCGAATTCGGCCTGATTCGTTGCCGTGTTCAGGTCGAGGTCCGCTGGCTAC AGCGCCTGGCAGCCCACCCGGGCATTCCGGAAGTCGCGCCCTTCTCCGCCGAAGCCAATGCGCTGTTGAATCAGCTGGCG GAGAACTTCCAGCTGGAGCACGCCGAGCGTGTCAAGGAATTCGAGCGCACCACCAACCACGACGTCAAAGCCGTGGAGTA CCTGCTCAAGGAGCAGGCCAAGCAGCTGCCTGAATTGGCCAAGGTCAACGAATTCATCCACTTCGCCTGCACCAGCGAAG ACATCAATAACCTGTCCCACGCGCTGATGTTGCGCGAAGGCCGCGACACCGTTCTGCTGCCGCTGATGCGCCAGCTCGCC GACGCCATCCGCGGCCTGGCCGTCCAGTTCGCCGACGTGCCGATGCTGTCGCGCACCCACGGTCAGCCGGCCTCCCCGAC TACGCTTGGCAAGGAACTGGCCAACGTGGTCTATCGCCTGGAGCGCCAGATCGCTCAGGTAGCTGCCGTGCCGCTGCTGG GCAAGATCAACGGGGCCGTGGGCAACTACAACGCCCACCTGTCCGCCTACCCGGACGTCGACTGGGAAGCCAACGCCCGC GAATTCATCGAGGGTGATCTGGGCCTGTCGTGGAACCCCTACACCACGCAGATCGAGCCGCACGACTACATCGCCGAGCT GTTCGACGCCATCGCACGCTTCAACACCATCCTCATCGACTTCGATCGCGACGTCTGGGGCTACATCTCCCTCGGTTACT TCAAGCAGAAAACCGTCGCAGGCGAAATCGGCTCCTCGACCATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACTCT GAAGGAAACCTCGGCATTGCCAACGCGCTATTCCAGCACCTGGCCAGCAAGCTGCCGATTTCCCGCTGGCAGCGCGATCT GACCGACTCCACCGTCCTGCGCAACCTGGGCGTGGGCTTCGCCCACAGCGTCATTGCCTACGAGGCGAGCCTCAAGGGTA TCGGCAAGCTGGAGCTCAACGCTGCCCGCATCGCCGAAGACCTCGATGCCTGCTGGGAAGTGCTTGCCGAGCCGGTACAG ACCGTCATGCGGCGTTATGCCGTGGAGAATGCCTACGAGAAGCTGAAGGATCTGACCCGCGGCAAAGGCATTACGCCTGA CGCACTGCAGGCCTTCATCGACGGCCTCGACATCCCGGCCGACGCCAAGGCGGAGCTGCGCAAGCTGACGCCGGCAAGCT ACATCGGCAACGCCGCTGCCCAGGCCAAGCGCATCTGA
Upstream 100 bases:
>100_bases CTCTATCCTCGACTGCGCTCGACACAGAGCGAAGACCGATAACCGGGGAGAATGGACCGGGATGTGCCGGTACATTCGTG TATAATCTGCGCCCTTTTCG
Downstream 100 bases:
>100_bases TCGACCCCATGCCTGCACGCCCGGCTGTGCCGGGCGTTTTTATTTTCAGCTCTGCCATATAGAGCAAGGTGTTACGCATG ACTTCTGATATTCCACTTCA
Product: adenylosuccinate lyase
Products: NA
Alternate protein names: ASL; Adenylosuccinase; ASase [H]
Number of amino acids: Translated: 465; Mature: 464
Protein sequence:
>465_residues MPDCPRMPSMQLSSLTAVSPVDGRYAGKTSSLRPIFSEFGLIRCRVQVEVRWLQRLAAHPGIPEVAPFSAEANALLNQLA ENFQLEHAERVKEFERTTNHDVKAVEYLLKEQAKQLPELAKVNEFIHFACTSEDINNLSHALMLREGRDTVLLPLMRQLA DAIRGLAVQFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPDVDWEANAR EFIEGDLGLSWNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVAGEIGSSTMPHKVNPIDFENS EGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGIGKLELNAARIAEDLDACWEVLAEPVQ TVMRRYAVENAYEKLKDLTRGKGITPDALQAFIDGLDIPADAKAELRKLTPASYIGNAAAQAKRI
Sequences:
>Translated_465_residues MPDCPRMPSMQLSSLTAVSPVDGRYAGKTSSLRPIFSEFGLIRCRVQVEVRWLQRLAAHPGIPEVAPFSAEANALLNQLA ENFQLEHAERVKEFERTTNHDVKAVEYLLKEQAKQLPELAKVNEFIHFACTSEDINNLSHALMLREGRDTVLLPLMRQLA DAIRGLAVQFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPDVDWEANAR EFIEGDLGLSWNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVAGEIGSSTMPHKVNPIDFENS EGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGIGKLELNAARIAEDLDACWEVLAEPVQ TVMRRYAVENAYEKLKDLTRGKGITPDALQAFIDGLDIPADAKAELRKLTPASYIGNAAAQAKRI >Mature_464_residues PDCPRMPSMQLSSLTAVSPVDGRYAGKTSSLRPIFSEFGLIRCRVQVEVRWLQRLAAHPGIPEVAPFSAEANALLNQLAE NFQLEHAERVKEFERTTNHDVKAVEYLLKEQAKQLPELAKVNEFIHFACTSEDINNLSHALMLREGRDTVLLPLMRQLAD AIRGLAVQFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPDVDWEANARE FIEGDLGLSWNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVAGEIGSSTMPHKVNPIDFENSE GNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGIGKLELNAARIAEDLDACWEVLAEPVQT VMRRYAVENAYEKLKDLTRGKGITPDALQAFIDGLDIPADAKAELRKLTPASYIGNAAAQAKRI
Specific function: De novo purine biosynthesis; eighth step. [C]
COG id: COG0015
COG function: function code F; Adenylosuccinate lyase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]
Homologues:
Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=68.3741648106904, Blast_Score=665, Evalue=0.0,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013539 - InterPro: IPR000362 - InterPro: IPR020557 - InterPro: IPR008948 - InterPro: IPR022761 - InterPro: IPR004769 [H]
Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]
EC number: =4.3.2.2 [H]
Molecular weight: Translated: 51527; Mature: 51396
Theoretical pI: Translated: 5.82; Mature: 5.82
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPDCPRMPSMQLSSLTAVSPVDGRYAGKTSSLRPIFSEFGLIRCRVQVEVRWLQRLAAHP CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHHHCCC GIPEVAPFSAEANALLNQLAENFQLEHAERVKEFERTTNHDVKAVEYLLKEQAKQLPELA CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHH KVNEFIHFACTSEDINNLSHALMLREGRDTVLLPLMRQLADAIRGLAVQFADVPMLSRTH HHHHHHHEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC GQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPDVDWEANAR CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHH EFIEGDLGLSWNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVA HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHH GEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGF HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH AHSVIAYEASLKGIGKLELNAARIAEDLDACWEVLAEPVQTVMRRYAVENAYEKLKDLTR HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC GKGITPDALQAFIDGLDIPADAKAELRKLTPASYIGNAAAQAKRI CCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCC >Mature Secondary Structure PDCPRMPSMQLSSLTAVSPVDGRYAGKTSSLRPIFSEFGLIRCRVQVEVRWLQRLAAHP CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHHHCCC GIPEVAPFSAEANALLNQLAENFQLEHAERVKEFERTTNHDVKAVEYLLKEQAKQLPELA CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHH KVNEFIHFACTSEDINNLSHALMLREGRDTVLLPLMRQLADAIRGLAVQFADVPMLSRTH HHHHHHHEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC GQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPDVDWEANAR CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHH EFIEGDLGLSWNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVA HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHH GEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGF HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH AHSVIAYEASLKGIGKLELNAARIAEDLDACWEVLAEPVQTVMRRYAVENAYEKLKDLTR HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC GKGITPDALQAFIDGLDIPADAKAELRKLTPASYIGNAAAQAKRI CCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]