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The map label for this gene is cysI [H]

Identifier: 146282584

GI number: 146282584

Start: 2439535

End: 2441193

Strand: Direct

Name: cysI [H]

Synonym: PST_2236

Alternate gene names: 146282584

Gene position: 2439535-2441193 (Clockwise)

Preceding gene: 146282583

Following gene: 146282585

Centisome position: 53.41

GC content: 62.45

Gene sequence:

>1659_bases
ATGTACGTTTACGACCAGTACGACCAACACATCATCGAGGATCGGGTCCGGCAGTTCCGCGACCAGACCCGCCGCTTCCT
GGCCGGTGAACTCGCTGATGAGGAATTCCGCCCGCTGCGCCTGCAGAATGGCCTGTACATCCAGCGCTACGCGCCCATGC
TGCGCATTGCCGTGCCATATGGCCTGCTTTCCTCGACGCAGGTGCGCAAGCTGGCAGAGATCGCTCGTCGCTACGACAAG
GGCTATGCCCATATCAGTACCCGTACCAACGTGCAGTTCAACTGGCCTGAGCTGGAAGACGTACCCGAGATTCTCGCCGA
ACTGGCCACGGTGCAGATGCACGCTATCCAGACCAGCGGCAACTGCATCCGCAACACCACCACCGATCAGTTCGCCGGCG
TGGCTCGTGATGAGCTGATCGACCCGCGGCCCTGGTGCGAAATCATCCGCCAGTGGTCCACCTTCCACCCCGAATTCGCC
TTCCTGCCGCGCAAGTTCAAGATCGCCGTCAACGGCGCAGTGAGCGATCGCGCTGCGATCGAAGTGCACGATATCGGCCT
GGAAGCGGTGAAGAACGATGCAGGCGAGCTGGGCTTCCGCGTTTCGGTCGGTGGCGGCCTCGGCCGGACGCCGATCGTCG
GCAGCTTCATCAACGAATTCCTGCCGTGGCAGCACCTGCTGAGCTACCTCGATGCCATCCTGCGGGTTTACAACCGTTAC
GGCCGCCGCGACAACAAGTTCAAGGCGCGCATCAAGATCCTGGTCAAGGCGCTGACGCCTGAAGTGTTCGCCGAGCGCGT
CAACGCCGAGTGGGCGCACTTGAAAGACGGCCCCACCACCCTGACCGAAGCCGAAGTGCAGCGCGTCAGCAAGTTCTTCG
TCGATCCGCAGTACAAGGCTCTGCAGGATCAGGACACCGCCCTCGCCCAGCTGGACGCCGAACATCCCGGTTTCGGCCGC
TGGCGCCAGCGCAACGTGGTGGCGCACAAGAAGCCCGGCTACGCCGCCGTGACGCTGTCGCTGAAGAGTACCGGCGTGGC
CCCTGGCGACGTCACTGACAAGCAGCTCGACGCCATCGCCGATCTGGCCGATCGCTACAGCTTTGGCGAGGTGCGCAACT
CCCACGAGCAGAACATGATCCTGGCGGACGTAGAGCAGGCCAGGCTGTTCGAGCTGTGGCACGAACTGCGCGAGCTGGGT
CTGGCCACGCCGAACATCGGCTTGCTGACCGACATCATCTGCTGCCCGGGCGGCGATTTCTGCTCGCTGGCCAACGCCAA
GTCGATCCCGATCGCCGAGGCCATCCAGCGTCGCTTCGACAACCTCGATTACCTGTTCGACCTGGGCAACATCGACCTGA
ACATCTCCGGTTGCATGAACGCCTGTGGCCATCACCATATCGGCCACATCGGCATCCTAGGCGTGGACAAGAAAGGCGCC
GAGTTCTACCAGGTCTCGCTGGGCGGCAGCTCCGGCCGCGACGCCAGCCTCGGCCAGATCCTCGGCCCATCCTTCGCCCA
GGATGCGATGCCCGACGTGATCGAGAAGATCATCACCGTCTACGTGGAGCAGCGTACCGAAGATGAGCAGTTCATCGATA
CTTTCCGCCGCATCGGCATTGATCCCTTCAAGGAGCGCGTCTATGCAGCGAATCATTAA

Upstream 100 bases:

>100_bases
CACTTTTGCGCATAACAAGTAGCTGGTATCGTGCCGCCCTCCCTAAATGGGTGCGCGGCCGTGCGCGCGAAAGCTGTAGC
AGCCTGAGAACAGGACATTT

Downstream 100 bases:

>100_bases
GAACGACCAGCTGGTCGACGAAACCTGGCACCTGCTCCCCAAGGAGCAGACCCTGGACGACCTGTGCAATAGCGACGATC
TGATCGTGCCGCTGCACCTG

Product: sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MYVYDQYDQHIIEDRVRQFRDQTRRFLAGELADEEFRPLRLQNGLYIQRYAPMLRIAVPYGLLSSTQVRKLAEIARRYDK
GYAHISTRTNVQFNWPELEDVPEILAELATVQMHAIQTSGNCIRNTTTDQFAGVARDELIDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVNGAVSDRAAIEVHDIGLEAVKNDAGELGFRVSVGGGLGRTPIVGSFINEFLPWQHLLSYLDAILRVYNRY
GRRDNKFKARIKILVKALTPEVFAERVNAEWAHLKDGPTTLTEAEVQRVSKFFVDPQYKALQDQDTALAQLDAEHPGFGR
WRQRNVVAHKKPGYAAVTLSLKSTGVAPGDVTDKQLDAIADLADRYSFGEVRNSHEQNMILADVEQARLFELWHELRELG
LATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDNLDYLFDLGNIDLNISGCMNACGHHHIGHIGILGVDKKGA
EFYQVSLGGSSGRDASLGQILGPSFAQDAMPDVIEKIITVYVEQRTEDEQFIDTFRRIGIDPFKERVYAANH

Sequences:

>Translated_552_residues
MYVYDQYDQHIIEDRVRQFRDQTRRFLAGELADEEFRPLRLQNGLYIQRYAPMLRIAVPYGLLSSTQVRKLAEIARRYDK
GYAHISTRTNVQFNWPELEDVPEILAELATVQMHAIQTSGNCIRNTTTDQFAGVARDELIDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVNGAVSDRAAIEVHDIGLEAVKNDAGELGFRVSVGGGLGRTPIVGSFINEFLPWQHLLSYLDAILRVYNRY
GRRDNKFKARIKILVKALTPEVFAERVNAEWAHLKDGPTTLTEAEVQRVSKFFVDPQYKALQDQDTALAQLDAEHPGFGR
WRQRNVVAHKKPGYAAVTLSLKSTGVAPGDVTDKQLDAIADLADRYSFGEVRNSHEQNMILADVEQARLFELWHELRELG
LATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDNLDYLFDLGNIDLNISGCMNACGHHHIGHIGILGVDKKGA
EFYQVSLGGSSGRDASLGQILGPSFAQDAMPDVIEKIITVYVEQRTEDEQFIDTFRRIGIDPFKERVYAANH
>Mature_552_residues
MYVYDQYDQHIIEDRVRQFRDQTRRFLAGELADEEFRPLRLQNGLYIQRYAPMLRIAVPYGLLSSTQVRKLAEIARRYDK
GYAHISTRTNVQFNWPELEDVPEILAELATVQMHAIQTSGNCIRNTTTDQFAGVARDELIDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVNGAVSDRAAIEVHDIGLEAVKNDAGELGFRVSVGGGLGRTPIVGSFINEFLPWQHLLSYLDAILRVYNRY
GRRDNKFKARIKILVKALTPEVFAERVNAEWAHLKDGPTTLTEAEVQRVSKFFVDPQYKALQDQDTALAQLDAEHPGFGR
WRQRNVVAHKKPGYAAVTLSLKSTGVAPGDVTDKQLDAIADLADRYSFGEVRNSHEQNMILADVEQARLFELWHELRELG
LATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDNLDYLFDLGNIDLNISGCMNACGHHHIGHIGILGVDKKGA
EFYQVSLGGSSGRDASLGQILGPSFAQDAMPDVIEKIITVYVEQRTEDEQFIDTFRRIGIDPFKERVYAANH

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62316; Mature: 62316

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVYDQYDQHIIEDRVRQFRDQTRRFLAGELADEEFRPLRLQNGLYIQRYAPMLRIAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHCCHHHHHHCC
GLLSSTQVRKLAEIARRYDKGYAHISTRTNVQFNWPELEDVPEILAELATVQMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHEEECCC
NCIRNTTTDQFAGVARDELIDPRPWCEIIRQWSTFHPEFAFLPRKFKIAVNGAVSDRAAI
CEEECCCHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCCCCCEE
EVHDIGLEAVKNDAGELGFRVSVGGGLGRTPIVGSFINEFLPWQHLLSYLDAILRVYNRY
EEECCCHHHHHCCHHHCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKFKARIKILVKALTPEVFAERVNAEWAHLKDGPTTLTEAEVQRVSKFFVDPQYKA
CCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCHHH
LQDQDTALAQLDAEHPGFGRWRQRNVVAHKKPGYAAVTLSLKSTGVAPGDVTDKQLDAIA
HCCCCHHHHHHCCCCCCCCCHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
DLADRYSFGEVRNSHEQNMILADVEQARLFELWHELRELGLATPNIGLLTDIICCPGGDF
HHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
CSLANAKSIPIAEAIQRRFDNLDYLFDLGNIDLNISGCMNACGHHHIGHIGILGVDKKGA
HCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHCCCCCCCEEEEEEECCCCC
EFYQVSLGGSSGRDASLGQILGPSFAQDAMPDVIEKIITVYVEQRTEDEQFIDTFRRIGI
EEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
DPFKERVYAANH
CHHHHHHHCCCC
>Mature Secondary Structure
MYVYDQYDQHIIEDRVRQFRDQTRRFLAGELADEEFRPLRLQNGLYIQRYAPMLRIAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHCCHHHHHHCC
GLLSSTQVRKLAEIARRYDKGYAHISTRTNVQFNWPELEDVPEILAELATVQMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHEEECCC
NCIRNTTTDQFAGVARDELIDPRPWCEIIRQWSTFHPEFAFLPRKFKIAVNGAVSDRAAI
CEEECCCHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCCCCCEE
EVHDIGLEAVKNDAGELGFRVSVGGGLGRTPIVGSFINEFLPWQHLLSYLDAILRVYNRY
EEECCCHHHHHCCHHHCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKFKARIKILVKALTPEVFAERVNAEWAHLKDGPTTLTEAEVQRVSKFFVDPQYKA
CCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCHHH
LQDQDTALAQLDAEHPGFGRWRQRNVVAHKKPGYAAVTLSLKSTGVAPGDVTDKQLDAIA
HCCCCHHHHHHCCCCCCCCCHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
DLADRYSFGEVRNSHEQNMILADVEQARLFELWHELRELGLATPNIGLLTDIICCPGGDF
HHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
CSLANAKSIPIAEAIQRRFDNLDYLFDLGNIDLNISGCMNACGHHHIGHIGILGVDKKGA
HCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHCCCCCCCEEEEEEECCCCC
EFYQVSLGGSSGRDASLGQILGPSFAQDAMPDVIEKIITVYVEQRTEDEQFIDTFRRIGI
EEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
DPFKERVYAANH
CHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]