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The map label for this gene is hsdM [H]

Identifier: 145301150

GI number: 145301150

Start: 4625928

End: 4627364

Strand: Direct

Name: hsdM [H]

Synonym: ASA_4326

Alternate gene names: 145301150

Gene position: 4625928-4627364 (Clockwise)

Preceding gene: 145301148

Following gene: 145301151

Centisome position: 98.37

GC content: 42.87

Gene sequence:

>1437_bases
ATGAAGCAAGATAACATCATTCAAAAGATATGGGGGCTTTGTAACATACTTCGTGGTGATGGGATCACTTATTACCAGTA
TGTATCTGAATTAAGCTATCTACTATTTCTTAAAATTGCCCAGGAAAATGGCTCAGAAAAACTGATACCCAAAGGCTATC
GCTGGATTGATCTGGAATCTCATACAGAAGATGGACTATTAGGTTTTTATCAGGAAATGCTTACTCACTTAGGTGCTTAT
GTTGAAAATGAAGTTGTAAGGGCAATTTATGCATTTCCTACAACAGTTTTCTCTCATTCCGAAAACTTAAAAGCTGTCAT
AAATGGTATTTCAAAAATAGAGTGGCACCAAGTAGGAAAAGATGGATTTGGAGAATTATACAGCGGTTTAATTGACAAGA
GTGCTCAAGATACTCGTTCAGGTGCAGGTCAGTACTTCACTCCACGCTCTTTAGTTAACACTATATTGAGATTAATACAG
CCCAACCTTGGCGAATTAATTCAAGACCCTGCAACCGGAAGCGGTGGCTTCTTGGTTTCTGCTGATAATTATATTAGAAA
CAAATATCCACGAGAAAAATACAAAGCTAATCCTCCTAAATGTCAAGGGGTGGAAATAGAGAAAAATACTCGCCGAATTT
GCTTAATGAATACATTTCTACATGAATTAGATGCAAATATTATTTATGGTGATGCACTGACTGATGACGTGGCTGAATTG
GCAGAAGCAGATGTAATCATAGCTAACCCTCCTTTTGGTAATAAAGCTGGGGGGCAACGTCCTTTGCGTAACGATATTCC
ATTCCCGAATGCGAACAAGCAACTCGCTTTTTTACAGCATATTTATTTGGGGCTCAAACCCGGTGGCCGTGCTGCCGTAG
TATTGCCTGACAACGTGTTATTTGAAGCCGGTGTCGGCACCGAGGTACGCCGCGACCTGATGAACAAGTGCAACCTGCAT
ACTATTTTGCGGTTGCCCACCGGTATCTTTTACGCTCAGGGCGTAAAAACCAACGTTCTGTTTTTTACCAAGGGATCAGC
CAAAGACAAATATCAGGAAGAAAGCTGCACTCAGAATGTCTGGGTCTACGATCTACGTACCAATATGCCAAGCTTTGGCA
AGCGAACCCCCTTTGGCAATTCTGATATTGGCTTTACGCCAGAAGAGTTGGGCACAGACCCACACCTGGGTGCCTTTGAG
AAAGTGTTTGGTGATAAGCCTGACGGCACTAGCAAACGCATCGAAGGTGAATTTAGCTTCAGCGCACAGGAGATCGAGGT
CGATAAAGATGCCGAGGAAGAAAACCAGGGGATCGATGACCGACTCGCACATTCCAGCTGGCGCTGTTTTAGCCGTTATT
GGATCGCCGAGACCAAAGGTGACTCGCTGGATATCTCATGGCTAAAAGATAAAGACAGTGTAGATGCAGCTAAATAG

Upstream 100 bases:

>100_bases
GGAACATTTAGCTTCGTATTTTTTATGCAATGCATGAAGGTTCTTGATGTAAAAGAAGTTAGACTATAAATTTAATATTA
ATAGACTACTGGAGTTGTTA

Downstream 100 bases:

>100_bases
CTGTTCCGAATAGTAGATCACCGGAGGAAGGGAACTCAGTCCAGTTTGTGCGATCTCATTAGTCGCCAAACAAAACAAGC
CACACCCAAAGGACTGAGTT

Product: type I restriction-modification system M subunit

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKQDNIIQKIWGLCNILRGDGITYYQYVSELSYLLFLKIAQENGSEKLIPKGYRWIDLESHTEDGLLGFYQEMLTHLGAY
VENEVVRAIYAFPTTVFSHSENLKAVINGISKIEWHQVGKDGFGELYSGLIDKSAQDTRSGAGQYFTPRSLVNTILRLIQ
PNLGELIQDPATGSGGFLVSADNYIRNKYPREKYKANPPKCQGVEIEKNTRRICLMNTFLHELDANIIYGDALTDDVAEL
AEADVIIANPPFGNKAGGQRPLRNDIPFPNANKQLAFLQHIYLGLKPGGRAAVVLPDNVLFEAGVGTEVRRDLMNKCNLH
TILRLPTGIFYAQGVKTNVLFFTKGSAKDKYQEESCTQNVWVYDLRTNMPSFGKRTPFGNSDIGFTPEELGTDPHLGAFE
KVFGDKPDGTSKRIEGEFSFSAQEIEVDKDAEEENQGIDDRLAHSSWRCFSRYWIAETKGDSLDISWLKDKDSVDAAK

Sequences:

>Translated_478_residues
MKQDNIIQKIWGLCNILRGDGITYYQYVSELSYLLFLKIAQENGSEKLIPKGYRWIDLESHTEDGLLGFYQEMLTHLGAY
VENEVVRAIYAFPTTVFSHSENLKAVINGISKIEWHQVGKDGFGELYSGLIDKSAQDTRSGAGQYFTPRSLVNTILRLIQ
PNLGELIQDPATGSGGFLVSADNYIRNKYPREKYKANPPKCQGVEIEKNTRRICLMNTFLHELDANIIYGDALTDDVAEL
AEADVIIANPPFGNKAGGQRPLRNDIPFPNANKQLAFLQHIYLGLKPGGRAAVVLPDNVLFEAGVGTEVRRDLMNKCNLH
TILRLPTGIFYAQGVKTNVLFFTKGSAKDKYQEESCTQNVWVYDLRTNMPSFGKRTPFGNSDIGFTPEELGTDPHLGAFE
KVFGDKPDGTSKRIEGEFSFSAQEIEVDKDAEEENQGIDDRLAHSSWRCFSRYWIAETKGDSLDISWLKDKDSVDAAK
>Mature_478_residues
MKQDNIIQKIWGLCNILRGDGITYYQYVSELSYLLFLKIAQENGSEKLIPKGYRWIDLESHTEDGLLGFYQEMLTHLGAY
VENEVVRAIYAFPTTVFSHSENLKAVINGISKIEWHQVGKDGFGELYSGLIDKSAQDTRSGAGQYFTPRSLVNTILRLIQ
PNLGELIQDPATGSGGFLVSADNYIRNKYPREKYKANPPKCQGVEIEKNTRRICLMNTFLHELDANIIYGDALTDDVAEL
AEADVIIANPPFGNKAGGQRPLRNDIPFPNANKQLAFLQHIYLGLKPGGRAAVVLPDNVLFEAGVGTEVRRDLMNKCNLH
TILRLPTGIFYAQGVKTNVLFFTKGSAKDKYQEESCTQNVWVYDLRTNMPSFGKRTPFGNSDIGFTPEELGTDPHLGAFE
KVFGDKPDGTSKRIEGEFSFSAQEIEVDKDAEEENQGIDDRLAHSSWRCFSRYWIAETKGDSLDISWLKDKDSVDAAK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=494, Percent_Identity=48.3805668016194, Blast_Score=470, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 53607; Mature: 53607

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDNIIQKIWGLCNILRGDGITYYQYVSELSYLLFLKIAQENGSEKLIPKGYRWIDLES
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
HTEDGLLGFYQEMLTHLGAYVENEVVRAIYAFPTTVFSHSENLKAVINGISKIEWHQVGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
DGFGELYSGLIDKSAQDTRSGAGQYFTPRSLVNTILRLIQPNLGELIQDPATGSGGFLVS
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEE
ADNYIRNKYPREKYKANPPKCQGVEIEKNTRRICLMNTFLHELDANIIYGDALTDDVAEL
CCHHHHCCCCHHHHCCCCCCCCCEEECCCCCEEEHHHHHHHHHCCCEEECCCHHHHHHHH
AEADVIIANPPFGNKAGGQRPLRNDIPFPNANKQLAFLQHIYLGLKPGGRAAVVLPDNVL
HHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEE
FEAGVGTEVRRDLMNKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDKYQEESCTQNV
EECCCCHHHHHHHHHHCCCEEEECCCCCCEEECCCCEEEEEEECCCCCCHHHHHHCCCCE
WVYDLRTNMPSFGKRTPFGNSDIGFTPEELGTDPHLGAFEKVFGDKPDGTSKRIEGEFSF
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCEECCCCCC
SAQEIEVDKDAEEENQGIDDRLAHSSWRCFSRYWIAETKGDSLDISWLKDKDSVDAAK
CCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKQDNIIQKIWGLCNILRGDGITYYQYVSELSYLLFLKIAQENGSEKLIPKGYRWIDLES
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
HTEDGLLGFYQEMLTHLGAYVENEVVRAIYAFPTTVFSHSENLKAVINGISKIEWHQVGK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
DGFGELYSGLIDKSAQDTRSGAGQYFTPRSLVNTILRLIQPNLGELIQDPATGSGGFLVS
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEE
ADNYIRNKYPREKYKANPPKCQGVEIEKNTRRICLMNTFLHELDANIIYGDALTDDVAEL
CCHHHHCCCCHHHHCCCCCCCCCEEECCCCCEEEHHHHHHHHHCCCEEECCCHHHHHHHH
AEADVIIANPPFGNKAGGQRPLRNDIPFPNANKQLAFLQHIYLGLKPGGRAAVVLPDNVL
HHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEE
FEAGVGTEVRRDLMNKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDKYQEESCTQNV
EECCCCHHHHHHHHHHCCCEEEECCCCCCEEECCCCEEEEEEECCCCCCHHHHHHCCCCE
WVYDLRTNMPSFGKRTPFGNSDIGFTPEELGTDPHLGAFEKVFGDKPDGTSKRIEGEFSF
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCEECCCCCC
SAQEIEVDKDAEEENQGIDDRLAHSSWRCFSRYWIAETKGDSLDISWLKDKDSVDAAK
CCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]