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The map label for this gene is livK [H]

Identifier: 145301110

GI number: 145301110

Start: 4578709

End: 4579830

Strand: Direct

Name: livK [H]

Synonym: ASA_4279

Alternate gene names: 145301110

Gene position: 4578709-4579830 (Clockwise)

Preceding gene: 145301108

Following gene: 145301111

Centisome position: 97.37

GC content: 60.34

Gene sequence:

>1122_bases
ATGAATAAGTTGAGCAAAATTGCAGTCAGGACAGCGGTTTCTATGGCTCTGTTCGGATCGGTATCGCTGGCCCATGCGGC
GGACACCATCAAGATCGGCATCGCCGGCCCTGTGACCGGACCGGTCGCCCAGTACGGTGACATGCAGTTCACCGGTGGCA
AGATGGCGGTCGAACAGATCAACAAGGCTGGCGGTATCGCAGGCAAGCAGATCGAAGCCGTCATCTATGACGATGCCTGC
GATCCCAAGCAGGCTGTGGCGATCGCCAACAAGGTGATCAACGACGGGGTCAAGTTCGTGGTCGGCCACCTCTGCTCCGA
CAATACGCTGCCAGCCTCCGACATCTATGAAGATGAAGGCGTGCTGATGATCACCCCTGCTTCCACCAACCCCAAGATCA
CCGAACGTGGCTACCAGCTGACCCTGCGCACCATTGGTCTGGACAGCGATCAGGGCCCGACTGCCGCCAAGTACATCATC
GAGCAGGTCAAGCCCCAGCGCGTGGCCGTCATCCACGACAAGAAGCAGTACGGTGAAGGCATCGCCTCCAGCGTGAAAGC
CTCGCTGGATAAAGCCGGCGTGAAAGTGGTGGCGTTCGAAGGCATCACAGCCGGTGACAAAGACTTCTCCGCCCTGATCG
CCAAACTGCAGAAAGAGAACGTCGACTTCATCTACTACGGCGGCTATCACCCCGAGCTGGGTCTGATCCTGCGCCAGGCG
GCCGAGAAAGGGCTCAAGGCCAAGTTCATGGGTCCGGAAGGGGTAGGTAACAAGGACATCTCCGCCATCGGTGGCCCGGC
TTCAGAAGGCCTGCTGGTCACCCTGCCGAACAAATACGATCTGGTGCCGGCCAACGCCCCGCTGGTCGCAGCGTTCAAGG
CCAAGGGCCAGGACTCTTCCGGTCCCTTCGTCTGGACCACCTACGCCGCGGTGCAAACCCTGGCGGCCGGTATCGCCAAG
GCGGGTGAAGATGACCCGGCTGCCGTCGCTGCCGCCATCAAGCGCACGCCGATCGAGACCGTCATGGGTACCCTGAGCTG
GGCACCGAGTGGCGACCTGAAAGGCTTTGAATTCGGTGTGTTCCAGTGGCACGCCGACGGTACCTCCACTCAAGTCAAAT
AA

Upstream 100 bases:

>100_bases
GCGAGGAATGATAGAACCATACCTATTGCCGGACTGGCGGGATCCTGGTTCGGAATGACACACGGAAAATGAAACAATCC
ACTGATTAAGGGACGAATCG

Downstream 100 bases:

>100_bases
TCTGCCGGTCGGGGTGACGTGAGTCACCCCTCCCTTCTTGCGGGCAAGCACAGGGAACGTAGTATGTCCGAACACCTCCT
TTACCTGGTTCAGCAACTGC

Product: ABC-type branched-chain amino acid transporter, periplasmic binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNKLSKIAVRTAVSMALFGSVSLAHAADTIKIGIAGPVTGPVAQYGDMQFTGGKMAVEQINKAGGIAGKQIEAVIYDDAC
DPKQAVAIANKVINDGVKFVVGHLCSDNTLPASDIYEDEGVLMITPASTNPKITERGYQLTLRTIGLDSDQGPTAAKYII
EQVKPQRVAVIHDKKQYGEGIASSVKASLDKAGVKVVAFEGITAGDKDFSALIAKLQKENVDFIYYGGYHPELGLILRQA
AEKGLKAKFMGPEGVGNKDISAIGGPASEGLLVTLPNKYDLVPANAPLVAAFKAKGQDSSGPFVWTTYAAVQTLAAGIAK
AGEDDPAAVAAAIKRTPIETVMGTLSWAPSGDLKGFEFGVFQWHADGTSTQVK

Sequences:

>Translated_373_residues
MNKLSKIAVRTAVSMALFGSVSLAHAADTIKIGIAGPVTGPVAQYGDMQFTGGKMAVEQINKAGGIAGKQIEAVIYDDAC
DPKQAVAIANKVINDGVKFVVGHLCSDNTLPASDIYEDEGVLMITPASTNPKITERGYQLTLRTIGLDSDQGPTAAKYII
EQVKPQRVAVIHDKKQYGEGIASSVKASLDKAGVKVVAFEGITAGDKDFSALIAKLQKENVDFIYYGGYHPELGLILRQA
AEKGLKAKFMGPEGVGNKDISAIGGPASEGLLVTLPNKYDLVPANAPLVAAFKAKGQDSSGPFVWTTYAAVQTLAAGIAK
AGEDDPAAVAAAIKRTPIETVMGTLSWAPSGDLKGFEFGVFQWHADGTSTQVK
>Mature_373_residues
MNKLSKIAVRTAVSMALFGSVSLAHAADTIKIGIAGPVTGPVAQYGDMQFTGGKMAVEQINKAGGIAGKQIEAVIYDDAC
DPKQAVAIANKVINDGVKFVVGHLCSDNTLPASDIYEDEGVLMITPASTNPKITERGYQLTLRTIGLDSDQGPTAAKYII
EQVKPQRVAVIHDKKQYGEGIASSVKASLDKAGVKVVAFEGITAGDKDFSALIAKLQKENVDFIYYGGYHPELGLILRQA
AEKGLKAKFMGPEGVGNKDISAIGGPASEGLLVTLPNKYDLVPANAPLVAAFKAKGQDSSGPFVWTTYAAVQTLAAGIAK
AGEDDPAAVAAAIKRTPIETVMGTLSWAPSGDLKGFEFGVFQWHADGTSTQVK

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=63.1578947368421, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1789867, Length=368, Percent_Identity=58.9673913043478, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39040; Mature: 39040

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLSKIAVRTAVSMALFGSVSLAHAADTIKIGIAGPVTGPVAQYGDMQFTGGKMAVEQI
CCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCHHHCCCEEECCCHHHHHHH
NKAGGIAGKQIEAVIYDDACDPKQAVAIANKVINDGVKFVVGHLCSDNTLPASDIYEDEG
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCC
VLMITPASTNPKITERGYQLTLRTIGLDSDQGPTAAKYIIEQVKPQRVAVIHDKKQYGEG
EEEEECCCCCCCEECCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCH
IASSVKASLDKAGVKVVAFEGITAGDKDFSALIAKLQKENVDFIYYGGYHPELGLILRQA
HHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AEKGLKAKFMGPEGVGNKDISAIGGPASEGLLVTLPNKYDLVPANAPLVAAFKAKGQDSS
HHCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEECCCCEEEECCCCCEEEEEECCCCCCC
GPFVWTTYAAVQTLAAGIAKAGEDDPAAVAAAIKRTPIETVMGTLSWAPSGDLKGFEFGV
CCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEE
FQWHADGTSTQVK
EEEECCCCCCCCC
>Mature Secondary Structure
MNKLSKIAVRTAVSMALFGSVSLAHAADTIKIGIAGPVTGPVAQYGDMQFTGGKMAVEQI
CCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCHHHCCCEEECCCHHHHHHH
NKAGGIAGKQIEAVIYDDACDPKQAVAIANKVINDGVKFVVGHLCSDNTLPASDIYEDEG
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCC
VLMITPASTNPKITERGYQLTLRTIGLDSDQGPTAAKYIIEQVKPQRVAVIHDKKQYGEG
EEEEECCCCCCCEECCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCH
IASSVKASLDKAGVKVVAFEGITAGDKDFSALIAKLQKENVDFIYYGGYHPELGLILRQA
HHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AEKGLKAKFMGPEGVGNKDISAIGGPASEGLLVTLPNKYDLVPANAPLVAAFKAKGQDSS
HHCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEECCCCEEEECCCCCEEEEEECCCCCCC
GPFVWTTYAAVQTLAAGIAKAGEDDPAAVAAAIKRTPIETVMGTLSWAPSGDLKGFEFGV
CCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEE
FQWHADGTSTQVK
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA