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The map label for this gene is glyS [H]

Identifier: 145301092

GI number: 145301092

Start: 4556639

End: 4558708

Strand: Direct

Name: glyS [H]

Synonym: ASA_4261

Alternate gene names: 145301092

Gene position: 4556639-4558708 (Clockwise)

Preceding gene: 145301091

Following gene: 145301095

Centisome position: 96.9

GC content: 63.0

Gene sequence:

>2070_bases
ATGGCACAACATACATTTCTGGTAGAGATCGGCACAGCTGAGCTGCCGCCAAAAGCCCTGCGCTCCTTGGCCGAAGCCTT
TGCCGACAACTTCAAAGCTGAGCTGACCAAGGCCGATCTGGCCTTCGGCGACATCGAGTGGTTTGCCTCACCGCGCCGCC
TGGCGCTGAAGGTACACGCGCTGGCCAGCGAGCAGCCGAGCAAGAGCGTCGAGAAGCGCGGCCCGGCCGTCTCCCAGGCG
TTCGATGCCGAAGGCAAGCCGACCAAGGCTGCCGAAGGCTGGGCCCGTGGCAACGGCATCACGGTTGAACAGGCCGAGCG
TCTGGTCACCGACAAGGGTGAGTGGCTGGTTCACACCGCCAAGGTCGAAGGCCGTCCGGCCAAAGATCTGCTGGGCGAGC
TGGTCGCCTCTGCACTGGCCAAGCTGCCTATCCCCAAGATGATGCGCTGGGGCGACAAGACCATCCAGTTCGTGCGTCCG
GTCTTTACCGTGACCCTGCTGCTGGATGGCGAGCTGGTGCCCGCTCACATTCTGGGTATCGACTCTGCCCGCACAATTCG
CGGTCATCGCTTTATGGGCGAGCCCGAGTTCACCATCGACAACGCCAGCCAGTACCCGCAGATCCTGCTGGAAAAAGGCA
AAGTGGTTGCCGACTTCATGGCGCGCAAGGCCAAGATCAAGGCTGACGCCGAAGCGGCCGCTGCCGCCTTCGGTGGCGTT
GCCGATCTGGACGACGCCCTGCTGGAAGAGGTCACCGCACTGGTCGAGTGGCCGGTCGTGCTGACCGCCAACTTCGAAGA
GAAGTTCCTGGCCGTGCCGGCTGAGGCGCTGGTGCACACCATGAAGGGTGACCAGAAGTACTTCCCGGTCTACGACAAGA
ACGGCAAGCTGCTGCCGAAGTTCATCTTCGTCACCAACATAGAGTCGAAAGACCCGAGCCAGATCATCAGCGGCAACGAG
AAGGTGGTGCGCCCCCGTCTCTCTGACGCCGAGTTCTTCTTCAAGACCGACCTGAAGCAGACTCTGGCCTCCCGCCTGCC
GCGCCTTGAAACCGTGCTGTTCCAGCAACAGCTGGGCACAGTCAAGGCCAAGGTCGAGCGCATCGAGACCGTCGCCGGCT
TTATCGCCGAGCGCATCGGCGCCAACGTGGCTCAGGCCAAGCGTGCCGGTCTGCTCTCCAAGTGTGATCTCATGACCAAC
ATGGTCGGCGAGTTCGCCAGCACCCAGGGTGTGATGGGGATGCACTACGCCCGTCACGATGGCGAAGACGAAGTGGTGGC
CGTGGCCCTGAACGAGCAGTACATGCCGCGCTTCGCCGGTGATGCCCTGCCGTCCGCTCTGGAAGCCTGCGCCGTTGCGC
TGGCCGACAAGCTCGACACCCTGGCCGGTATCTTCGGCATCGGCATGCTGCCCAAAGGCGATAAAGACCCCTTCGCCCTG
CGCCGCGCCGCCATCGGTGCCCTGCGCATCATGACCGAGAAGCAGCTGGATCTGGATCTGGTTGAACTGGTTGAAGAAGC
GGTACGCGTCTACGGCGACAAGCTGACCAACAAAACCGTCGTCACCGACGTGGTCGACTTTATGCTGGGCCGCTTCCGCG
CCGCCTATCAGGACGAAGGCATAGGTGCCGATGTGGTGCTGGCCGTACTGGCCCGCCGCCCGACCCGTCCGCTCGACTTC
GATCGTCGCGTCAAGGCCGTCAGCCACTTCCGCAGTCTGGATGCCGCACTGGCATTGGCTGCTGCCAACAAGCGCGTCAG
CAACATTCTGGCCAAGGTCGAAGGCGAACTGCCGACTGCCGTCAAACCGGAACTGCTGCAAGACGCTGCCGAGAAGGCGC
TGGCCACCCAGGTTGCCGAACTGCAAGCCGAACTGGCCCCGCTGTTCGCTGCCGGTGACTATCAGGCTGCCCTCACCCGT
CTGGCCGCCCTGCGCGAGCCGGTCGACACCTTCTTCAACGAAGTGATGGTGATGGCCGACGACGAAGCCCTCAAGGCCAA
CCGTCTGGCGTTGCTGAACAACCTGCGCAACCTGTTCCTGCAAGTCGCGGATATCTCCCTGCTGCAGTGA

Upstream 100 bases:

>100_bases
CCTGACCAAGGCCGTGGCCGAGGCCTACTACGCCTCCCGCGAAGCGCTGGGTTTCCCCATGTGCAAGCGCACTGAGAACA
AGCAAGGTTAAGGAACGGAC

Downstream 100 bases:

>100_bases
TTCTGGTTGAGTAAGTGATATAAAAAACGGCGCCGCGAGGCGCCGTTTTTTATTATCAATACCGGGAAGCAAGTTCCTCA
CAAAAAGGCACCAGTGGCAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MAQHTFLVEIGTAELPPKALRSLAEAFADNFKAELTKADLAFGDIEWFASPRRLALKVHALASEQPSKSVEKRGPAVSQA
FDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHTAKVEGRPAKDLLGELVASALAKLPIPKMMRWGDKTIQFVRP
VFTVTLLLDGELVPAHILGIDSARTIRGHRFMGEPEFTIDNASQYPQILLEKGKVVADFMARKAKIKADAEAAAAAFGGV
ADLDDALLEEVTALVEWPVVLTANFEEKFLAVPAEALVHTMKGDQKYFPVYDKNGKLLPKFIFVTNIESKDPSQIISGNE
KVVRPRLSDAEFFFKTDLKQTLASRLPRLETVLFQQQLGTVKAKVERIETVAGFIAERIGANVAQAKRAGLLSKCDLMTN
MVGEFASTQGVMGMHYARHDGEDEVVAVALNEQYMPRFAGDALPSALEACAVALADKLDTLAGIFGIGMLPKGDKDPFAL
RRAAIGALRIMTEKQLDLDLVELVEEAVRVYGDKLTNKTVVTDVVDFMLGRFRAAYQDEGIGADVVLAVLARRPTRPLDF
DRRVKAVSHFRSLDAALALAAANKRVSNILAKVEGELPTAVKPELLQDAAEKALATQVAELQAELAPLFAAGDYQAALTR
LAALREPVDTFFNEVMVMADDEALKANRLALLNNLRNLFLQVADISLLQ

Sequences:

>Translated_689_residues
MAQHTFLVEIGTAELPPKALRSLAEAFADNFKAELTKADLAFGDIEWFASPRRLALKVHALASEQPSKSVEKRGPAVSQA
FDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHTAKVEGRPAKDLLGELVASALAKLPIPKMMRWGDKTIQFVRP
VFTVTLLLDGELVPAHILGIDSARTIRGHRFMGEPEFTIDNASQYPQILLEKGKVVADFMARKAKIKADAEAAAAAFGGV
ADLDDALLEEVTALVEWPVVLTANFEEKFLAVPAEALVHTMKGDQKYFPVYDKNGKLLPKFIFVTNIESKDPSQIISGNE
KVVRPRLSDAEFFFKTDLKQTLASRLPRLETVLFQQQLGTVKAKVERIETVAGFIAERIGANVAQAKRAGLLSKCDLMTN
MVGEFASTQGVMGMHYARHDGEDEVVAVALNEQYMPRFAGDALPSALEACAVALADKLDTLAGIFGIGMLPKGDKDPFAL
RRAAIGALRIMTEKQLDLDLVELVEEAVRVYGDKLTNKTVVTDVVDFMLGRFRAAYQDEGIGADVVLAVLARRPTRPLDF
DRRVKAVSHFRSLDAALALAAANKRVSNILAKVEGELPTAVKPELLQDAAEKALATQVAELQAELAPLFAAGDYQAALTR
LAALREPVDTFFNEVMVMADDEALKANRLALLNNLRNLFLQVADISLLQ
>Mature_688_residues
AQHTFLVEIGTAELPPKALRSLAEAFADNFKAELTKADLAFGDIEWFASPRRLALKVHALASEQPSKSVEKRGPAVSQAF
DAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHTAKVEGRPAKDLLGELVASALAKLPIPKMMRWGDKTIQFVRPV
FTVTLLLDGELVPAHILGIDSARTIRGHRFMGEPEFTIDNASQYPQILLEKGKVVADFMARKAKIKADAEAAAAAFGGVA
DLDDALLEEVTALVEWPVVLTANFEEKFLAVPAEALVHTMKGDQKYFPVYDKNGKLLPKFIFVTNIESKDPSQIISGNEK
VVRPRLSDAEFFFKTDLKQTLASRLPRLETVLFQQQLGTVKAKVERIETVAGFIAERIGANVAQAKRAGLLSKCDLMTNM
VGEFASTQGVMGMHYARHDGEDEVVAVALNEQYMPRFAGDALPSALEACAVALADKLDTLAGIFGIGMLPKGDKDPFALR
RAAIGALRIMTEKQLDLDLVELVEEAVRVYGDKLTNKTVVTDVVDFMLGRFRAAYQDEGIGADVVLAVLARRPTRPLDFD
RRVKAVSHFRSLDAALALAAANKRVSNILAKVEGELPTAVKPELLQDAAEKALATQVAELQAELAPLFAAGDYQAALTRL
AALREPVDTFFNEVMVMADDEALKANRLALLNNLRNLFLQVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=71.5529753265602, Blast_Score=980, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75146; Mature: 75014

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTFLVEIGTAELPPKALRSLAEAFADNFKAELTKADLAFGDIEWFASPRRLALKVHA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHH
LASEQPSKSVEKRGPAVSQAFDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHTA
HHCCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
KVEGRPAKDLLGELVASALAKLPIPKMMRWGDKTIQFVRPVFTVTLLLDGELVPAHILGI
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEECCCCCCHHEEEC
DSARTIRGHRFMGEPEFTIDNASQYPQILLEKGKVVADFMARKAKIKADAEAAAAAFGGV
CCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCH
ADLDDALLEEVTALVEWPVVLTANFEEKFLAVPAEALVHTMKGDQKYFPVYDKNGKLLPK
HHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHHHHHHHHCCCCEECCEECCCCCCCEE
FIFVTNIESKDPSQIISGNEKVVRPRLSDAEFFFKTDLKQTLASRLPRLETVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VKAKVERIETVAGFIAERIGANVAQAKRAGLLSKCDLMTNMVGEFASTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
GEDEVVAVALNEQYMPRFAGDALPSALEACAVALADKLDTLAGIFGIGMLPKGDKDPFAL
CCCCEEEEEECCHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRAAIGALRIMTEKQLDLDLVELVEEAVRVYGDKLTNKTVVTDVVDFMLGRFRAAYQDEG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
IGADVVLAVLARRPTRPLDFDRRVKAVSHFRSLDAALALAAANKRVSNILAKVEGELPTA
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VKPELLQDAAEKALATQVAELQAELAPLFAAGDYQAALTRLAALREPVDTFFNEVMVMAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
DEALKANRLALLNNLRNLFLQVADISLLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQHTFLVEIGTAELPPKALRSLAEAFADNFKAELTKADLAFGDIEWFASPRRLALKVHA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHH
LASEQPSKSVEKRGPAVSQAFDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHTA
HHCCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
KVEGRPAKDLLGELVASALAKLPIPKMMRWGDKTIQFVRPVFTVTLLLDGELVPAHILGI
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEECCCCCCHHEEEC
DSARTIRGHRFMGEPEFTIDNASQYPQILLEKGKVVADFMARKAKIKADAEAAAAAFGGV
CCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCH
ADLDDALLEEVTALVEWPVVLTANFEEKFLAVPAEALVHTMKGDQKYFPVYDKNGKLLPK
HHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHHHHHHHHCCCCEECCEECCCCCCCEE
FIFVTNIESKDPSQIISGNEKVVRPRLSDAEFFFKTDLKQTLASRLPRLETVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VKAKVERIETVAGFIAERIGANVAQAKRAGLLSKCDLMTNMVGEFASTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
GEDEVVAVALNEQYMPRFAGDALPSALEACAVALADKLDTLAGIFGIGMLPKGDKDPFAL
CCCCEEEEEECCHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRAAIGALRIMTEKQLDLDLVELVEEAVRVYGDKLTNKTVVTDVVDFMLGRFRAAYQDEG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
IGADVVLAVLARRPTRPLDFDRRVKAVSHFRSLDAALALAAANKRVSNILAKVEGELPTA
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VKPELLQDAAEKALATQVAELQAELAPLFAAGDYQAALTRLAALREPVDTFFNEVMVMAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
DEALKANRLALLNNLRNLFLQVADISLLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA