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The map label for this gene is fadA [H]

Identifier: 145301082

GI number: 145301082

Start: 4547911

End: 4549074

Strand: Direct

Name: fadA [H]

Synonym: ASA_4251

Alternate gene names: 145301082

Gene position: 4547911-4549074 (Clockwise)

Preceding gene: 145301081

Following gene: 145301083

Centisome position: 96.71

GC content: 64.43

Gene sequence:

>1164_bases
ATGAAAGACGTAGTCATTGTCGACTGTATCCGGACCCCCATGGGCCGGTCCAAGGGCGGCGCCTTCCGCAACGTGCGTGC
AGAAGATCTGTCCGCGCACCTGATGAGCTCCATCCTGCTGCGCAACCCCAACCTCGACCCGAACGAGATCGAGGACATCT
ACTGGGGCTGCGTGCAGCAGACCCTGGAACAGGGCTTCAACATAGCCCGCAACGCCGCCCTGCTGGCGGGGATCCCCAAG
CAGGTAGGTGCTGTCACCGTCAACCGGCTGTGTGGCTCCTCCATGCAGGCGCTGCATGATGCTTCCCGTGCCATTCAGGT
GGGTGATGGGGATATCTTCATCATCGGCGGCGTCGAGCACATGGGCCATGTGCCCATGAGCCACGGGGTCGACTTCCACC
CCGGCATGGCCAAGTCGGTGGCCAAGGCCTCCGGCATGATGGGGCTGACCGCCGAGATGCTGGGCAAGCTGCACGGCATC
AGCCGCCAGCAGCAGGACGAGTTTGCCGCCCGCTCCCACCGCCGCGCCTATGCCGCGACCGTGGAAGGGCGCTTCGCCAG
AGAGATCGTCGGGCTGGAAGGGCATGACGCCAGCGGTGCCCGCTTCTTCTACGACTACGACGAGGTGATCCGCCCGGAGA
CCACGGTCGAAACCCTGAGCCAGCTGCGCCCTGTGTTCGACCCGGTCAACGGCACAGTGACCGCCGGTACCTCCTCGGCC
CTGTCCGACGGCGCCTCCGCCATGCTGGTGATGAGCGCGGATCGTGCCAAGGCGCTGGGGCTGACTCCGCGCGTCAGAGT
ACGCTCCATGGCCGTGGCCGGTTGCGATGCCGCCATCATGGGTTATGGCCCTGTGCCTGCTACCCAAAAGGCGCTCAAGC
GTGCCGGTCTGACCATAGGCGACATCGATCTGGTCGAGCTGAACGAGGCGTTCGCCGCCCAGTCACTGCCTTGCGTCAAG
GATCTGGGACTGCTGGACGTGGCGGAAGAGAAGGTCAACCTGAACGGTGGTGCCATCGCTCTGGGTCACCCGCTGGGATG
CTCCGGCTCCCGCATCTCCACCACACTGATCCACCTGATGGAGGAGAAGGACGCCAATCTGGGTCTTGCCACCATGTGTA
TCGGTCTGGGCCAGGGCATAGCCACAGTGTTTGAACGGGTGTGA

Upstream 100 bases:

>100_bases
GTATGCCGATCTGGGCCCGCTCTACCGTGTCAGCGACCGCTTGCGCGAGATGGCTGCCCAGGGCAAAACCTTCTACTGAT
TGCGGAAAGGAGATCCATTC

Downstream 100 bases:

>100_bases
TGTCAACGGCTCGCCCTCACTGCGACAGTGAGGGCGAGCCAGAATTGTCTTTTTTGGGGTCGGCCTTGCCGGCCCGTCAT
CGAGTCTGTCGAGATCGCCA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKDVVIVDCIRTPMGRSKGGAFRNVRAEDLSAHLMSSILLRNPNLDPNEIEDIYWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMQALHDASRAIQVGDGDIFIIGGVEHMGHVPMSHGVDFHPGMAKSVAKASGMMGLTAEMLGKLHGI
SRQQQDEFAARSHRRAYAATVEGRFAREIVGLEGHDASGARFFYDYDEVIRPETTVETLSQLRPVFDPVNGTVTAGTSSA
LSDGASAMLVMSADRAKALGLTPRVRVRSMAVAGCDAAIMGYGPVPATQKALKRAGLTIGDIDLVELNEAFAAQSLPCVK
DLGLLDVAEEKVNLNGGAIALGHPLGCSGSRISTTLIHLMEEKDANLGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MKDVVIVDCIRTPMGRSKGGAFRNVRAEDLSAHLMSSILLRNPNLDPNEIEDIYWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMQALHDASRAIQVGDGDIFIIGGVEHMGHVPMSHGVDFHPGMAKSVAKASGMMGLTAEMLGKLHGI
SRQQQDEFAARSHRRAYAATVEGRFAREIVGLEGHDASGARFFYDYDEVIRPETTVETLSQLRPVFDPVNGTVTAGTSSA
LSDGASAMLVMSADRAKALGLTPRVRVRSMAVAGCDAAIMGYGPVPATQKALKRAGLTIGDIDLVELNEAFAAQSLPCVK
DLGLLDVAEEKVNLNGGAIALGHPLGCSGSRISTTLIHLMEEKDANLGLATMCIGLGQGIATVFERV
>Mature_387_residues
MKDVVIVDCIRTPMGRSKGGAFRNVRAEDLSAHLMSSILLRNPNLDPNEIEDIYWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMQALHDASRAIQVGDGDIFIIGGVEHMGHVPMSHGVDFHPGMAKSVAKASGMMGLTAEMLGKLHGI
SRQQQDEFAARSHRRAYAATVEGRFAREIVGLEGHDASGARFFYDYDEVIRPETTVETLSQLRPVFDPVNGTVTAGTSSA
LSDGASAMLVMSADRAKALGLTPRVRVRSMAVAGCDAAIMGYGPVPATQKALKRAGLTIGDIDLVELNEAFAAQSLPCVK
DLGLLDVAEEKVNLNGGAIALGHPLGCSGSRISTTLIHLMEEKDANLGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=38.4422110552764, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=40.5541561712846, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=37.6262626262626, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=33.6683417085427, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=32.9466357308585, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI194353979, Length=240, Percent_Identity=33.75, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=74.4186046511628, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787663, Length=406, Percent_Identity=46.3054187192118, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=43.640897755611, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.645885286783, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=32.2966507177034, Blast_Score=181, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=40.8521303258145, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=33.9152119700748, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=32.1016166281755, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=31.9899244332494, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=27.0408163265306, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=38.9873417721519, Blast_Score=222, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=33.3333333333333, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=39.9002493765586, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=37.5, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=32.4189526184539, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40977; Mature: 40977

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVVIVDCIRTPMGRSKGGAFRNVRAEDLSAHLMSSILLRNPNLDPNEIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMQALHDASRAIQVGDGDIFIIGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEEECHHH
MGHVPMSHGVDFHPGMAKSVAKASGMMGLTAEMLGKLHGISRQQQDEFAARSHRRAYAAT
HCCCCHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEE
VEGRFAREIVGLEGHDASGARFFYDYDEVIRPETTVETLSQLRPVFDPVNGTVTAGTSSA
CCCHHHHHHHCCCCCCCCCCEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCHH
LSDGASAMLVMSADRAKALGLTPRVRVRSMAVAGCDAAIMGYGPVPATQKALKRAGLTIG
HHCCCCEEEEEECCCHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEC
DIDLVELNEAFAAQSLPCVKDLGLLDVAEEKVNLNGGAIALGHPLGCSGSRISTTLIHLM
CEEEEECHHHHHHCCCCHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EEKDANLGLATMCIGLGQGIATVFERV
HHCCCCCCHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKDVVIVDCIRTPMGRSKGGAFRNVRAEDLSAHLMSSILLRNPNLDPNEIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMQALHDASRAIQVGDGDIFIIGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEEECHHH
MGHVPMSHGVDFHPGMAKSVAKASGMMGLTAEMLGKLHGISRQQQDEFAARSHRRAYAAT
HCCCCHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEE
VEGRFAREIVGLEGHDASGARFFYDYDEVIRPETTVETLSQLRPVFDPVNGTVTAGTSSA
CCCHHHHHHHCCCCCCCCCCEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCHH
LSDGASAMLVMSADRAKALGLTPRVRVRSMAVAGCDAAIMGYGPVPATQKALKRAGLTIG
HHCCCCEEEEEECCCHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEC
DIDLVELNEAFAAQSLPCVKDLGLLDVAEEKVNLNGGAIALGHPLGCSGSRISTTLIHLM
CEEEEECHHHHHHCCCCHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EEKDANLGLATMCIGLGQGIATVFERV
HHCCCCCCHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA