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The map label for this gene is mscS [H]

Identifier: 145301068

GI number: 145301068

Start: 4523758

End: 4524648

Strand: Direct

Name: mscS [H]

Synonym: ASA_4233

Alternate gene names: 145301068

Gene position: 4523758-4524648 (Clockwise)

Preceding gene: 145301067

Following gene: 145301070

Centisome position: 96.2

GC content: 57.46

Gene sequence:

>891_bases
ATGCCAGTGTTAGATTCATGCGTCATAAACCTTTACTGCCTGACAGGACATCCTATGGAACCCGAAATCATCACAGAAGC
GCAGACCTGGCTGGTCAATAATCAGGGCCTGCTGATCGAGTATGCCGTCAATATTGCGGCCGCCCTGATCACCCTGCTGG
TCGGTTACATCACCGCCAACCTTCTCAGCAATGGCGTGATCAAGGTGATGAATGCTCGCAAGCTGGATGCCACAGTGACG
CATTTCGTTGGCAGCATCCTCAAGTACACCATTCTGGTGTTCGTGGTGATTGCAGCCATGGGGCGAGTCGGGGTGCAAAC
CGCCTCCTTCGTCGCCATTATCGGTGCCGCCGGTCTGGCCATAGGTCTGGCACTGCAAGGCTCGCTCTCCAACTTCGCCG
CCGGTTTCCTGCTGATCATCTTCCGCCCCATCAAGGCCGGTGAGTTTATCGAAGTGGCGGGCACCAATGGCGTCGTGCAG
TCGGTGCAGCTATTCACTACCACCCTCACCTCGGGTGACAACAAGATGGTGGTGGTACCCAACTCCGCCATCCTCAACGG
CACCATAGTCAACTACTCGCGCATGGACACCCGCCGCGTCGACATGACCTTCGGTATCGGCTACGGTTCTGACCTGCGCA
AGGCCAAACAGCTCCTCGAGCGCCTGGTGAGTGAAGAGCCGCGCATCCTGAAGGAACAGGCAGCAACCATAGCCGTTGCC
GCCCTGGCCGACTCCTCGGTCAATATCGTAGTGCGTCCCTGGGTCAAGACTGCCGATTACTGGGGCGTCTGGTTCGACTT
CCACGAAAAGGTGAAGCTGACCTTCGATGCCGAAGGCATCGAGATCCCCTTCCCGCAGATGGTCATGCACATGCAAAAGC
CTCAGGCCTGA

Upstream 100 bases:

>100_bases
CTGACGCCATTCGGGTGGATACACAAAGGGAGGCCTTGGCCTCCCTTGTTTTTTATGGCAAAAAATGGAAGGGAGCACCA
GATGCGACCCAAACTGTCGC

Downstream 100 bases:

>100_bases
TACCTGCGCAGACGATCACGACAATGCCAGCCCTGAAGCTGGCATTGTCGTTTCTGAAACCCCGGTCGTGCCGCAAGGCA
TAAAGACTCGACCTGCAGCT

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPVLDSCVINLYCLTGHPMEPEIITEAQTWLVNNQGLLIEYAVNIAAALITLLVGYITANLLSNGVIKVMNARKLDATVT
HFVGSILKYTILVFVVIAAMGRVGVQTASFVAIIGAAGLAIGLALQGSLSNFAAGFLLIIFRPIKAGEFIEVAGTNGVVQ
SVQLFTTTLTSGDNKMVVVPNSAILNGTIVNYSRMDTRRVDMTFGIGYGSDLRKAKQLLERLVSEEPRILKEQAATIAVA
ALADSSVNIVVRPWVKTADYWGVWFDFHEKVKLTFDAEGIEIPFPQMVMHMQKPQA

Sequences:

>Translated_296_residues
MPVLDSCVINLYCLTGHPMEPEIITEAQTWLVNNQGLLIEYAVNIAAALITLLVGYITANLLSNGVIKVMNARKLDATVT
HFVGSILKYTILVFVVIAAMGRVGVQTASFVAIIGAAGLAIGLALQGSLSNFAAGFLLIIFRPIKAGEFIEVAGTNGVVQ
SVQLFTTTLTSGDNKMVVVPNSAILNGTIVNYSRMDTRRVDMTFGIGYGSDLRKAKQLLERLVSEEPRILKEQAATIAVA
ALADSSVNIVVRPWVKTADYWGVWFDFHEKVKLTFDAEGIEIPFPQMVMHMQKPQA
>Mature_295_residues
PVLDSCVINLYCLTGHPMEPEIITEAQTWLVNNQGLLIEYAVNIAAALITLLVGYITANLLSNGVIKVMNARKLDATVTH
FVGSILKYTILVFVVIAAMGRVGVQTASFVAIIGAAGLAIGLALQGSLSNFAAGFLLIIFRPIKAGEFIEVAGTNGVVQS
VQLFTTTLTSGDNKMVVVPNSAILNGTIVNYSRMDTRRVDMTFGIGYGSDLRKAKQLLERLVSEEPRILKEQAATIAVAA
LADSSVNIVVRPWVKTADYWGVWFDFHEKVKLTFDAEGIEIPFPQMVMHMQKPQA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=270, Percent_Identity=48.8888888888889, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1786670, Length=253, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787591, Length=242, Percent_Identity=29.3388429752066, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367355, Length=264, Percent_Identity=21.969696969697, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32101; Mature: 31970

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLDSCVINLYCLTGHPMEPEIITEAQTWLVNNQGLLIEYAVNIAAALITLLVGYITAN
CCCHHCEEEEEEEECCCCCCCHHEECCHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
LLSNGVIKVMNARKLDATVTHFVGSILKYTILVFVVIAAMGRVGVQTASFVAIIGAAGLA
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGLALQGSLSNFAAGFLLIIFRPIKAGEFIEVAGTNGVVQSVQLFTTTLTSGDNKMVVVP
HHHEECCCHHHHHHHHHHEEEECCCCCCEEEEECCCCHHHHHHHHEEEEECCCCEEEEEC
NSAILNGTIVNYSRMDTRRVDMTFGIGYGSDLRKAKQLLERLVSEEPRILKEQAATIAVA
CCEEECCEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEE
ALADSSVNIVVRPWVKTADYWGVWFDFHEKVKLTFDAEGIEIPFPQMVMHMQKPQA
EECCCCCEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
PVLDSCVINLYCLTGHPMEPEIITEAQTWLVNNQGLLIEYAVNIAAALITLLVGYITAN
CCHHCEEEEEEEECCCCCCCHHEECCHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
LLSNGVIKVMNARKLDATVTHFVGSILKYTILVFVVIAAMGRVGVQTASFVAIIGAAGLA
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGLALQGSLSNFAAGFLLIIFRPIKAGEFIEVAGTNGVVQSVQLFTTTLTSGDNKMVVVP
HHHEECCCHHHHHHHHHHEEEECCCCCCEEEEECCCCHHHHHHHHEEEEECCCCEEEEEC
NSAILNGTIVNYSRMDTRRVDMTFGIGYGSDLRKAKQLLERLVSEEPRILKEQAATIAVA
CCEEECCEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEE
ALADSSVNIVVRPWVKTADYWGVWFDFHEKVKLTFDAEGIEIPFPQMVMHMQKPQA
EECCCCCEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]