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The map label for this gene is sgaA [H]

Identifier: 145301051

GI number: 145301051

Start: 4509934

End: 4510377

Strand: Direct

Name: sgaA [H]

Synonym: ASA_4216

Alternate gene names: 145301051

Gene position: 4509934-4510377 (Clockwise)

Preceding gene: 145301047

Following gene: 145301052

Centisome position: 95.91

GC content: 62.84

Gene sequence:

>444_bases
ATGCTGACTCAACTGCTGACCGAAGAGGTCATTTCCATCGAAACGGGAGCCCGCGACTGGCGTCATGCCATCGAGCTCGC
CACCGCTCCCCTGCTGGCCAACGGCACCATAGAGCCTTCCTATGTGGCGGCGCTGTTTCGCTCCCACGAGGAGCTCGGTC
CCTACTATGTGCTGGGCCCGGGCCTGGCCATGCCCCACGCCCGCCCGGAAGATGGCGTCAATCGTCTCGGCCTTGGGCTG
ACAGTGCTGAAAACCGGAGTCAATTTCGGCTCCGAGGGCAATGACCCGATCCGGCTGCTCGTCACCCTGGCCGCCAGCGA
CAGCAACTCCCACGTGGAGACCATCGCCCAGCTGGCCGAACTGTTCATGAACGAAGAGGACGTGGCGGCCATCATGGCAG
CCGACACCAAAGACGACATTTTGCGCATTCTGGCGCGCTACTGA

Upstream 100 bases:

>100_bases
TTAGCGCTATCTGGTAGGGCTAACTTTCGAGATCAGGCTCACAGTTATCGTGGGGCAAGATCGCTAGATTGGCGCCATCT
CATCCAGCCGGATCATTGCC

Downstream 100 bases:

>100_bases
GCGGCGCCCTGCGCCCGACAAAGGAAGAGACGATGAAAATTCTGGTTGTATGCGGACACGGCCTGGGCACCAGCCTGATG
ATGGAGATGAGCATCAAGAG

Product: PTS system IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLTQLLTEEVISIETGARDWRHAIELATAPLLANGTIEPSYVAALFRSHEELGPYYVLGPGLAMPHARPEDGVNRLGLGL
TVLKTGVNFGSEGNDPIRLLVTLAASDSNSHVETIAQLAELFMNEEDVAAIMAADTKDDILRILARY

Sequences:

>Translated_147_residues
MLTQLLTEEVISIETGARDWRHAIELATAPLLANGTIEPSYVAALFRSHEELGPYYVLGPGLAMPHARPEDGVNRLGLGL
TVLKTGVNFGSEGNDPIRLLVTLAASDSNSHVETIAQLAELFMNEEDVAAIMAADTKDDILRILARY
>Mature_147_residues
MLTQLLTEEVISIETGARDWRHAIELATAPLLANGTIEPSYVAALFRSHEELGPYYVLGPGLAMPHARPEDGVNRLGLGL
TVLKTGVNFGSEGNDPIRLLVTLAASDSNSHVETIAQLAELFMNEEDVAAIMAADTKDDILRILARY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790755, Length=135, Percent_Identity=37.7777777777778, Blast_Score=118, Evalue=1e-28,
Organism=Escherichia coli, GI2367359, Length=137, Percent_Identity=38.6861313868613, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1789302, Length=115, Percent_Identity=44.3478260869565, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1790027, Length=142, Percent_Identity=32.3943661971831, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15912; Mature: 15912

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQLLTEEVISIETGARDWRHAIELATAPLLANGTIEPSYVAALFRSHEELGPYYVLGP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCEEEECC
GLAMPHARPEDGVNRLGLGLTVLKTGVNFGSEGNDPIRLLVTLAASDSNSHVETIAQLAE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LFMNEEDVAAIMAADTKDDILRILARY
HHCCCCHHHHEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MLTQLLTEEVISIETGARDWRHAIELATAPLLANGTIEPSYVAALFRSHEELGPYYVLGP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCEEEECC
GLAMPHARPEDGVNRLGLGLTVLKTGVNFGSEGNDPIRLLVTLAASDSNSHVETIAQLAE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LFMNEEDVAAIMAADTKDDILRILARY
HHCCCCHHHHEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]