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The map label for this gene is plsB [H]

Identifier: 145301043

GI number: 145301043

Start: 4502199

End: 4504658

Strand: Direct

Name: plsB [H]

Synonym: ASA_4207

Alternate gene names: 145301043

Gene position: 4502199-4504658 (Clockwise)

Preceding gene: 145301039

Following gene: 145301046

Centisome position: 95.74

GC content: 61.99

Gene sequence:

>2460_bases
ATGCTCACGCCGCATTTTTTTGGAGAGCAGACGCACATGTCCCTTGGACAGAAGATTTCCAGAGCCATCTTGCAATGGCC
GGTCAGTGGCTTGGTCAATCACAAGAGCCTGCCGGAAAATCCCATCACAGAGCTCAAGCTGGATCCAGCCAGACCCATAG
TCTATGCCCTGAAAACCAGCTCGATCACCGACCTGATGACACTGCAACAGTGCTGCGAGGATCTCGGCCTGCCCGGTCCC
TTCACCCCGCTGGAGCTGAACGGTCAGTTGCTGCCGCGCTATGTCTGTCTGGACCGCCCGCCTCCCCTGTTCGGCAAGCG
CAGCAAACCGCTGCCTTTCTTGCAGGAGTTCCACCAGCTGCTGGATCTGCACAAGCAGGATCCGGCACTGGATATCCAGG
TGGTGCCCGTCACCCTGTTCTGGGGTCGCGCCCCCGGCCGGGAAGGGGAAGAGGCCTCCGGCTGGAACATCATCAGCAGT
CTGGCACCGAACCGCCTCAAGAAGGCGCTGATCGTGATCCTCAAGGGCCGCGAGAACCTGGTGCGCTTCTCTCCGCCGCT
CTCCCTGCGTCAAATGGCGGACAAGCACGGCACCGACGAGGCTATCGCCCACAAGCTGGCGCGGGTGGCCCGCACCCACT
TCAGCCGCCAGCAGCTGGCAGCCACCGGCCCCAAGCTGCCGAACCGCAACCTGCTGTTCAGGCAGTTGCTGGACTCCAGC
GTGATCCAGCAGGCCATAGAAGAGGAGGCGCAGCGCGAGGGCATCAGCCTGGAGAAAGCGCAAAAGCGCGCCCACGGTTA
CATGGACGAGATCGCCTCCGATTTCTCCTACCGGCTGATCCGCCTCAGTGAAAGCTTCCTCGGCTGGCTGTGGAACAAGC
TCTATCGCGGCCTCTCGGTCAACGGTGCCGAGAAGGTACGCCAGCTGGCGCAAGAAGGGCACGAGATCGTCTATATGCCC
TGCCATCGCAGCCACATGGACTACCTGCTGCTCTCCTACGTCATCTACCACCAGGGGATGGTGCCGCCCCACATAGCGGC
GGGCATCAACCTCAACTTCTGGCCGGCGGGCCCCATCTTCCGTCATGGCGGCGCCTTCTTCATCCGCCGCACCTTCAAGG
GCAACCCGCTCTACAGCACTGTATTTCGCGAATACCTCAACCTGCTGTTTGCCAAGGGCTATTCGGTGGAGTTCTTTACC
GAGGGTGGGCGCTCCCGCACCGGTCGTCTGCTGCCACCCAAAACCGGCATGCTGGCAATGACACTGCAGGCCATGATGCG
AGGGCTGGACAGACCCGTGACCCTGGTGCCCGTCTATCTGGGCTACGAGCATGTGATGGAGGTGAACACCTATCACAACG
AGCTCAAGGGCAGCCGCAAGGAGAAAGAGAGCTTCCTGCAGGTGCTCGGCATACTGCGCAAGCTGCGCAACTATGGCCGC
GGCTTCGTCAACTTCGGCGAACCCCTGACCCTCAACAGCTACCTCAATGAACATGTGCCCGAGTGGAAGGACCACATAGG
TCAGGAGGAGCGCCCAGAGTGGATGGCACCCACAGTCACCCGGCTGGCCGAGCTGCTGATGACCCGCATCAACGCTGCCG
CCGCCGTCAATGGCCTGACCCTGAGCGCGCTGGCACTGCTGGCCGCCGAACGCCACGCCCTGACTCGCGATGAACTGCAG
GCACAGCTCAACACCTATCTGGACCTGCTCAAGCAGGTGCCCTACAGCCCCCAGAGCACAATCCCGGCTGAGGATGCCAA
GACACTGCTGGATCAGGCGATGGAGCTCAACAAGTTCGAGGTGAGCGAGGACAAGCTGGGCCAGATCATCAGCCTGGACC
GCTATCAGGCGATCCTGCTGACCTACTACCGCAACAACATACTGCACCTGTTTGCATTGCCATCCCTGGTGGCCGCGCTG
ATCGAACGTTGCGAAGGGATATCCCGCGGCGAGATCGTCGCCCGCTGCATCGACATCTATCCGCTGCTCAAGACGGAGCT
GTTCCTCCACTACGAAGAGGATGAGCTGCCGGAGCTGGTCGATGCCCTGCTGGCCGAACTGCAACGCCAGCAACTGGTCG
AGGCACGGGATGGCGGCTTCTGGGTCAATCCTGGCAACCAGATGCGCCTGCTGCTACTGGCCGAGAGCATCCAGGAGACG
CTGCAACGCTACGCCATAGTGTTGACCCGGGTGCTGGCCCAGCCCTATGTCGAGGCGGAGCAGCTGGAGGCGGACGGCCT
GATGATGGCGGAGCGCCTCGGCACCCTGCACGGCATCAATGCTCCCGAGTTCTTCGACCAGAAGCTGTTCAGCACCCTGA
TCCACACCCTGCGCAAGGAGGGCTACCTCTCTCCCGAGGGCAAACCGGATCTGGGCCGCTTCCAGGCGCTGGCCGACAAC
ATAGTGCCGCTGCTGAGCAACAAGATCCGCCGCACCATACAGGCGGGCAACCGGCTCTGA

Upstream 100 bases:

>100_bases
CGGCTGACATTTGTCGCGGCCAACCCCTTATTTGCCATGGTTTTACGGCTGCGCAGCCGACTCATCGTACCTCTCGGCGA
CAAGCCGCCGGTGATTTGTT

Downstream 100 bases:

>100_bases
CAGAGACCGGAAACAAAGCAGGCCGCATCATGCGGCCTTCTTTTTATCCTGACATGCGTCACGGCAACAGGTAGTTGAGC
ACCAGACCGATGAATACCAG

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MLTPHFFGEQTHMSLGQKISRAILQWPVSGLVNHKSLPENPITELKLDPARPIVYALKTSSITDLMTLQQCCEDLGLPGP
FTPLELNGQLLPRYVCLDRPPPLFGKRSKPLPFLQEFHQLLDLHKQDPALDIQVVPVTLFWGRAPGREGEEASGWNIISS
LAPNRLKKALIVILKGRENLVRFSPPLSLRQMADKHGTDEAIAHKLARVARTHFSRQQLAATGPKLPNRNLLFRQLLDSS
VIQQAIEEEAQREGISLEKAQKRAHGYMDEIASDFSYRLIRLSESFLGWLWNKLYRGLSVNGAEKVRQLAQEGHEIVYMP
CHRSHMDYLLLSYVIYHQGMVPPHIAAGINLNFWPAGPIFRHGGAFFIRRTFKGNPLYSTVFREYLNLLFAKGYSVEFFT
EGGRSRTGRLLPPKTGMLAMTLQAMMRGLDRPVTLVPVYLGYEHVMEVNTYHNELKGSRKEKESFLQVLGILRKLRNYGR
GFVNFGEPLTLNSYLNEHVPEWKDHIGQEERPEWMAPTVTRLAELLMTRINAAAAVNGLTLSALALLAAERHALTRDELQ
AQLNTYLDLLKQVPYSPQSTIPAEDAKTLLDQAMELNKFEVSEDKLGQIISLDRYQAILLTYYRNNILHLFALPSLVAAL
IERCEGISRGEIVARCIDIYPLLKTELFLHYEEDELPELVDALLAELQRQQLVEARDGGFWVNPGNQMRLLLLAESIQET
LQRYAIVLTRVLAQPYVEAEQLEADGLMMAERLGTLHGINAPEFFDQKLFSTLIHTLRKEGYLSPEGKPDLGRFQALADN
IVPLLSNKIRRTIQAGNRL

Sequences:

>Translated_819_residues
MLTPHFFGEQTHMSLGQKISRAILQWPVSGLVNHKSLPENPITELKLDPARPIVYALKTSSITDLMTLQQCCEDLGLPGP
FTPLELNGQLLPRYVCLDRPPPLFGKRSKPLPFLQEFHQLLDLHKQDPALDIQVVPVTLFWGRAPGREGEEASGWNIISS
LAPNRLKKALIVILKGRENLVRFSPPLSLRQMADKHGTDEAIAHKLARVARTHFSRQQLAATGPKLPNRNLLFRQLLDSS
VIQQAIEEEAQREGISLEKAQKRAHGYMDEIASDFSYRLIRLSESFLGWLWNKLYRGLSVNGAEKVRQLAQEGHEIVYMP
CHRSHMDYLLLSYVIYHQGMVPPHIAAGINLNFWPAGPIFRHGGAFFIRRTFKGNPLYSTVFREYLNLLFAKGYSVEFFT
EGGRSRTGRLLPPKTGMLAMTLQAMMRGLDRPVTLVPVYLGYEHVMEVNTYHNELKGSRKEKESFLQVLGILRKLRNYGR
GFVNFGEPLTLNSYLNEHVPEWKDHIGQEERPEWMAPTVTRLAELLMTRINAAAAVNGLTLSALALLAAERHALTRDELQ
AQLNTYLDLLKQVPYSPQSTIPAEDAKTLLDQAMELNKFEVSEDKLGQIISLDRYQAILLTYYRNNILHLFALPSLVAAL
IERCEGISRGEIVARCIDIYPLLKTELFLHYEEDELPELVDALLAELQRQQLVEARDGGFWVNPGNQMRLLLLAESIQET
LQRYAIVLTRVLAQPYVEAEQLEADGLMMAERLGTLHGINAPEFFDQKLFSTLIHTLRKEGYLSPEGKPDLGRFQALADN
IVPLLSNKIRRTIQAGNRL
>Mature_819_residues
MLTPHFFGEQTHMSLGQKISRAILQWPVSGLVNHKSLPENPITELKLDPARPIVYALKTSSITDLMTLQQCCEDLGLPGP
FTPLELNGQLLPRYVCLDRPPPLFGKRSKPLPFLQEFHQLLDLHKQDPALDIQVVPVTLFWGRAPGREGEEASGWNIISS
LAPNRLKKALIVILKGRENLVRFSPPLSLRQMADKHGTDEAIAHKLARVARTHFSRQQLAATGPKLPNRNLLFRQLLDSS
VIQQAIEEEAQREGISLEKAQKRAHGYMDEIASDFSYRLIRLSESFLGWLWNKLYRGLSVNGAEKVRQLAQEGHEIVYMP
CHRSHMDYLLLSYVIYHQGMVPPHIAAGINLNFWPAGPIFRHGGAFFIRRTFKGNPLYSTVFREYLNLLFAKGYSVEFFT
EGGRSRTGRLLPPKTGMLAMTLQAMMRGLDRPVTLVPVYLGYEHVMEVNTYHNELKGSRKEKESFLQVLGILRKLRNYGR
GFVNFGEPLTLNSYLNEHVPEWKDHIGQEERPEWMAPTVTRLAELLMTRINAAAAVNGLTLSALALLAAERHALTRDELQ
AQLNTYLDLLKQVPYSPQSTIPAEDAKTLLDQAMELNKFEVSEDKLGQIISLDRYQAILLTYYRNNILHLFALPSLVAAL
IERCEGISRGEIVARCIDIYPLLKTELFLHYEEDELPELVDALLAELQRQQLVEARDGGFWVNPGNQMRLLLLAESIQET
LQRYAIVLTRVLAQPYVEAEQLEADGLMMAERLGTLHGINAPEFFDQKLFSTLIHTLRKEGYLSPEGKPDLGRFQALADN
IVPLLSNKIRRTIQAGNRL

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=452, Percent_Identity=28.5398230088496, Blast_Score=155, Evalue=2e-37,
Organism=Homo sapiens, GI190358539, Length=280, Percent_Identity=29.6428571428571, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI87082362, Length=809, Percent_Identity=53.770086526576, Blast_Score=867, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=293, Percent_Identity=32.4232081911263, Blast_Score=145, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71988723, Length=563, Percent_Identity=25.9325044404973, Blast_Score=142, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71988728, Length=479, Percent_Identity=27.348643006263, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17864692, Length=284, Percent_Identity=34.1549295774648, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24650754, Length=504, Percent_Identity=27.5793650793651, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24650752, Length=504, Percent_Identity=27.5793650793651, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21357731, Length=504, Percent_Identity=27.5793650793651, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 92910; Mature: 92910

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPHFFGEQTHMSLGQKISRAILQWPVSGLVNHKSLPENPITELKLDPARPIVYALKTS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEECC
SITDLMTLQQCCEDLGLPGPFTPLELNGQLLPRYVCLDRPPPLFGKRSKPLPFLQEFHQL
CHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHH
LDLHKQDPALDIQVVPVTLFWGRAPGREGEEASGWNIISSLAPNRLKKALIVILKGRENL
HHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHHEEECCCCCC
VRFSPPLSLRQMADKHGTDEAIAHKLARVARTHFSRQQLAATGPKLPNRNLLFRQLLDSS
EEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VIQQAIEEEAQREGISLEKAQKRAHGYMDEIASDFSYRLIRLSESFLGWLWNKLYRGLSV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGAEKVRQLAQEGHEIVYMPCHRSHMDYLLLSYVIYHQGMVPPHIAAGINLNFWPAGPIF
CHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCCHH
RHGGAFFIRRTFKGNPLYSTVFREYLNLLFAKGYSVEFFTEGGRSRTGRLLPPKTGMLAM
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHH
TLQAMMRGLDRPVTLVPVYLGYEHVMEVNTYHNELKGSRKEKESFLQVLGILRKLRNYGR
HHHHHHHHCCCCEEEEEEHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GFVNFGEPLTLNSYLNEHVPEWKDHIGQEERPEWMAPTVTRLAELLMTRINAAAAVNGLT
CCCCCCCCEEHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LSALALLAAERHALTRDELQAQLNTYLDLLKQVPYSPQSTIPAEDAKTLLDQAMELNKFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VSEDKLGQIISLDRYQAILLTYYRNNILHLFALPSLVAALIERCEGISRGEIVARCIDIY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
PLLKTELFLHYEEDELPELVDALLAELQRQQLVEARDGGFWVNPGNQMRLLLLAESIQET
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHH
LQRYAIVLTRVLAQPYVEAEQLEADGLMMAERLGTLHGINAPEFFDQKLFSTLIHTLRKE
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GYLSPEGKPDLGRFQALADNIVPLLSNKIRRTIQAGNRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTPHFFGEQTHMSLGQKISRAILQWPVSGLVNHKSLPENPITELKLDPARPIVYALKTS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEECC
SITDLMTLQQCCEDLGLPGPFTPLELNGQLLPRYVCLDRPPPLFGKRSKPLPFLQEFHQL
CHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHH
LDLHKQDPALDIQVVPVTLFWGRAPGREGEEASGWNIISSLAPNRLKKALIVILKGRENL
HHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHHEEECCCCCC
VRFSPPLSLRQMADKHGTDEAIAHKLARVARTHFSRQQLAATGPKLPNRNLLFRQLLDSS
EEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VIQQAIEEEAQREGISLEKAQKRAHGYMDEIASDFSYRLIRLSESFLGWLWNKLYRGLSV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGAEKVRQLAQEGHEIVYMPCHRSHMDYLLLSYVIYHQGMVPPHIAAGINLNFWPAGPIF
CHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCCHH
RHGGAFFIRRTFKGNPLYSTVFREYLNLLFAKGYSVEFFTEGGRSRTGRLLPPKTGMLAM
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHH
TLQAMMRGLDRPVTLVPVYLGYEHVMEVNTYHNELKGSRKEKESFLQVLGILRKLRNYGR
HHHHHHHHCCCCEEEEEEHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GFVNFGEPLTLNSYLNEHVPEWKDHIGQEERPEWMAPTVTRLAELLMTRINAAAAVNGLT
CCCCCCCCEEHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LSALALLAAERHALTRDELQAQLNTYLDLLKQVPYSPQSTIPAEDAKTLLDQAMELNKFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VSEDKLGQIISLDRYQAILLTYYRNNILHLFALPSLVAALIERCEGISRGEIVARCIDIY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
PLLKTELFLHYEEDELPELVDALLAELQRQQLVEARDGGFWVNPGNQMRLLLLAESIQET
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHH
LQRYAIVLTRVLAQPYVEAEQLEADGLMMAERLGTLHGINAPEFFDQKLFSTLIHTLRKE
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GYLSPEGKPDLGRFQALADNIVPLLSNKIRRTIQAGNRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA