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The map label for this gene is amt [C]

Identifier: 145300869

GI number: 145300869

Start: 4309561

End: 4310802

Strand: Direct

Name: amt [C]

Synonym: ASA_4014

Alternate gene names: 145300869

Gene position: 4309561-4310802 (Clockwise)

Preceding gene: 145300868

Following gene: 145300870

Centisome position: 91.65

GC content: 62.24

Gene sequence:

>1242_bases
ATGGAAGAGTTGATCAAGTCCGTTTCAGAGCTGCGCTATGCGCTCGATACCTTCTATCTGCTGATATCCGGCGTGCTGGT
CATGTGGATGGCCGCCGGTTTTTCCATGCTCGAGGCCGGGCTGGTACGCGCCAAAAATACCACTGAGATCCTGACCAAGA
ATATTCTGCTCTATGCCATTGCCTGCATCATGTTCCTGCTGGTTGGCTACAACATCATGTACGTGGACAACGCGGCCGGT
GGCTGGTTGCCAAGCTTCGGCAGCCTGATCGGCACCCAGGCTGATGATGCCGGTCACTCACTGCACTCCGACTTCTTCTT
CCAGGTGGTGTTCGTGGCGACCGCTACCTCCATCGTCTCGGGGGCCGTGGCCGAGCGGATGAAACTCTGGTCCTTCCTGC
TGTTCTGCGTGGTGATGACGGGCGTCATCTATCCGATGGAAGGGTACTGGACCTGGGGCGGCGGCTTCCTCAAAGAGGCC
GGTTTCTCCGACTTCGCAGGCTCAGGCATAGTGCATTTGGCCGGTGCCGCCGCCGCCCTCGCCGCCGTGCTGCTGCTCGG
TGCCCGCCGCGGCAAATATGGCAAGCAGGGTGAGATCTACCCCATCCCGGGTTCCAACCTGCCGCTGGCAACCCTGGGTA
CCCTGATCCTCTGGATGGGCTGGTTCGGCTTCAACGGTGGCTCTCAGCTGGTGCTCTCCACCGCTGCCGACGCTACCGCC
ATCGGCACAGTGTTCGTCAACACCAATGCCGCAGCGGCAGCCGGTGTGGTCGGCGCCCTGCTGGTCAGCAAGCTCACCTG
GGGCAAGGCGGATCTCACCATGATCCTTAACGGCGCTCTCGGCGGCCTGGTTGCCATCACGGCCGATCCCGCCTCCCCCA
GCCCCCTGTTCGCCAGCCTGATCGGTGCCTTGGCCGGCGCCCTGGTGGTCTACGCCATCGTCGCCTTCGATCGCCTGAAG
ATTGATGATCCGGTCGGCGCCATCTCGGTACACGGTGTCTGCGGCCTGTTCGGCCTGATGGTAGTGCCCCTCTCCAACGA
GAGCGCCACCTTCTCGGCCCAGCTGCTGGGTGCGGCCGTCATCTTCGGCTGGGTGTTTGGTGCCTCCCTCATCGTCTGGG
GCCTGCTCAAGGCCACCATGGGGCTGCGAGTCAGCGAAGAGGAAGAGATGATGGGAGTCGACGTGCACGAATGCGGTATC
GATGCCTACCCCGAGTTCGTCACCGTCAAATCCAGCATCTGA

Upstream 100 bases:

>100_bases
GGCAAGATTTTCGTCTATGACCTGCAGGCTGCCGTGCGGATCCGCACCGGCGAGACTGACGCAGAAGCCCTTTGATACAA
CAGGAACCGAGGAACACAAC

Downstream 100 bases:

>100_bases
TCGACTGAACAAGCACAATGCAACAGGGCCGGAGGCAAACCTCCGGCCCTGTTTTTTATCGCCAGAAACGGGTGCTGTGT
GGATATCCTGGGTGCGCAGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MEELIKSVSELRYALDTFYLLISGVLVMWMAAGFSMLEAGLVRAKNTTEILTKNILLYAIACIMFLLVGYNIMYVDNAAG
GWLPSFGSLIGTQADDAGHSLHSDFFFQVVFVATATSIVSGAVAERMKLWSFLLFCVVMTGVIYPMEGYWTWGGGFLKEA
GFSDFAGSGIVHLAGAAAALAAVLLLGARRGKYGKQGEIYPIPGSNLPLATLGTLILWMGWFGFNGGSQLVLSTAADATA
IGTVFVNTNAAAAAGVVGALLVSKLTWGKADLTMILNGALGGLVAITADPASPSPLFASLIGALAGALVVYAIVAFDRLK
IDDPVGAISVHGVCGLFGLMVVPLSNESATFSAQLLGAAVIFGWVFGASLIVWGLLKATMGLRVSEEEEMMGVDVHECGI
DAYPEFVTVKSSI

Sequences:

>Translated_413_residues
MEELIKSVSELRYALDTFYLLISGVLVMWMAAGFSMLEAGLVRAKNTTEILTKNILLYAIACIMFLLVGYNIMYVDNAAG
GWLPSFGSLIGTQADDAGHSLHSDFFFQVVFVATATSIVSGAVAERMKLWSFLLFCVVMTGVIYPMEGYWTWGGGFLKEA
GFSDFAGSGIVHLAGAAAALAAVLLLGARRGKYGKQGEIYPIPGSNLPLATLGTLILWMGWFGFNGGSQLVLSTAADATA
IGTVFVNTNAAAAAGVVGALLVSKLTWGKADLTMILNGALGGLVAITADPASPSPLFASLIGALAGALVVYAIVAFDRLK
IDDPVGAISVHGVCGLFGLMVVPLSNESATFSAQLLGAAVIFGWVFGASLIVWGLLKATMGLRVSEEEEMMGVDVHECGI
DAYPEFVTVKSSI
>Mature_413_residues
MEELIKSVSELRYALDTFYLLISGVLVMWMAAGFSMLEAGLVRAKNTTEILTKNILLYAIACIMFLLVGYNIMYVDNAAG
GWLPSFGSLIGTQADDAGHSLHSDFFFQVVFVATATSIVSGAVAERMKLWSFLLFCVVMTGVIYPMEGYWTWGGGFLKEA
GFSDFAGSGIVHLAGAAAALAAVLLLGARRGKYGKQGEIYPIPGSNLPLATLGTLILWMGWFGFNGGSQLVLSTAADATA
IGTVFVNTNAAAAAGVVGALLVSKLTWGKADLTMILNGALGGLVAITADPASPSPLFASLIGALAGALVVYAIVAFDRLK
IDDPVGAISVHGVCGLFGLMVVPLSNESATFSAQLLGAAVIFGWVFGASLIVWGLLKATMGLRVSEEEEMMGVDVHECGI
DAYPEFVTVKSSI

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=408, Percent_Identity=32.3529411764706, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=438, Percent_Identity=33.7899543378995, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17550182, Length=425, Percent_Identity=31.7647058823529, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI32564042, Length=430, Percent_Identity=28.8372093023256, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI133931100, Length=342, Percent_Identity=31.2865497076023, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6325396, Length=425, Percent_Identity=28.2352941176471, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321559, Length=425, Percent_Identity=26.8235294117647, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=409, Percent_Identity=27.1393643031785, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078314, Length=404, Percent_Identity=32.9207920792079, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43244; Mature: 43244

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELIKSVSELRYALDTFYLLISGVLVMWMAAGFSMLEAGLVRAKNTTEILTKNILLYAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ACIMFLLVGYNIMYVDNAAGGWLPSFGSLIGTQADDAGHSLHSDFFFQVVFVATATSIVS
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GAVAERMKLWSFLLFCVVMTGVIYPMEGYWTWGGGFLKEAGFSDFAGSGIVHLAGAAAAL
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHH
AAVLLLGARRGKYGKQGEIYPIPGSNLPLATLGTLILWMGWFGFNGGSQLVLSTAADATA
HHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
IGTVFVNTNAAAAAGVVGALLVSKLTWGKADLTMILNGALGGLVAITADPASPSPLFASL
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
IGALAGALVVYAIVAFDRLKIDDPVGAISVHGVCGLFGLMVVPLSNESATFSAQLLGAAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHEEECCCCCCHHHHHHHHHHH
IFGWVFGASLIVWGLLKATMGLRVSEEEEMMGVDVHECGIDAYPEFVTVKSSI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCCCCEEEEECCC
>Mature Secondary Structure
MEELIKSVSELRYALDTFYLLISGVLVMWMAAGFSMLEAGLVRAKNTTEILTKNILLYAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ACIMFLLVGYNIMYVDNAAGGWLPSFGSLIGTQADDAGHSLHSDFFFQVVFVATATSIVS
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GAVAERMKLWSFLLFCVVMTGVIYPMEGYWTWGGGFLKEAGFSDFAGSGIVHLAGAAAAL
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHH
AAVLLLGARRGKYGKQGEIYPIPGSNLPLATLGTLILWMGWFGFNGGSQLVLSTAADATA
HHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
IGTVFVNTNAAAAAGVVGALLVSKLTWGKADLTMILNGALGGLVAITADPASPSPLFASL
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
IGALAGALVVYAIVAFDRLKIDDPVGAISVHGVCGLFGLMVVPLSNESATFSAQLLGAAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHEEECCCCCCHHHHHHHHHHH
IFGWVFGASLIVWGLLKATMGLRVSEEEEMMGVDVHECGIDAYPEFVTVKSSI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]