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The map label for this gene is rpfG [H]

Identifier: 145300784

GI number: 145300784

Start: 4211968

End: 4213098

Strand: Direct

Name: rpfG [H]

Synonym: ASA_3924

Alternate gene names: 145300784

Gene position: 4211968-4213098 (Clockwise)

Preceding gene: 145300783

Following gene: 145300786

Centisome position: 89.57

GC content: 59.5

Gene sequence:

>1131_bases
ATGCTCAACTTGCTGGATCCCGACACCAAACCCACACTGCTGGTGGTCGATGACACCCCTGAAAACCTCTCCCTGCTCAG
CGCCCTGCTGGGCCCGCACTATCGGGTCAAGGTTGCCATCAGTGGCCAGCGGGCGCTGGAGATACTGTCATCAGGCACCC
GGGTGGACCTGATCCTGCTCGATGTGGTGATGCCCCAGATGGATGGCTATCAGGTGATGCAGGCACTGCAGGAGATGCCG
CAGGCGCGGCATATTCCGGTGATTTTCCTGACCGCCAAGGTAGAGGAGAGCGCAGAAGCGCACGGACTGGCACTCGGCGC
CGTGGACTATATCCACAAGCCTATCTCTCCCGCCATCGTACTGGCCCGCGTCAGAAACCACCTTGAACTGAAAGCTGCAC
GTGATCTGCTCCATGACCAAAATGCCTATCTGGAACAGGAGGTGGAACGGCGCACCCGTGAAAACGAGCTGATCCAGAAC
ATCACCATCCACTCCCTCGCCTCTCTGGCCGAGACCCGGGACAACGAGACCGGCAACCACATACTGCGCACCCAGCACTA
TGTGAAGCTATTAGCGGAAACCCTGTGCGATCACCCTCGCTTTCGTGCAGAGCTGACCCCAAAGACCATAGATCTGCTGT
TCAAGTCTGCCCCCCTGCACGATATTGGCAAGGTCGGCGTACCGGACCGGATCCTGCTCAAACCCGGCAAGCTGGATGCG
GATGAGTTCACCCAGATGAAGCACCACTCCATCCTCGGCAAGGAGGCCATAGTCCACGCCGAGGAAGCGCTGGGGGTGGA
GGTCGAGTTTCTGCGACTGGCCAAGGAGATCGCCTGCTACCACCACGAGAAATGGGATGGCACCGGCTACCCCTACGGTC
TGATGGGGGATGAGATCCCCCTGTCGGCCCGACTGATGGCGCTGGCAGATGTGTACGATGCCCTGATCAGCAAGCGGTGT
TACAAGGCGGCCATGTCCCATACGGAGGTGGTCCCCATCATATTGGCGGGGCGCAGCAGTCACTTCGATCCCGACATCAC
GGATGCCTTCGCCCGCATCCACCTGCTGTTTCACGAAATTGCACTGCGCTACAACGATGACAAGGGCGAACAGGAGGCCA
ACCTCGACTAG

Upstream 100 bases:

>100_bases
AGACGCCATGACCCGCTTCCGTTTTACGGATGCACTGGCACACCTGCAACAAGTCCGGGCCCTCAGGCCCGATCTGTTCA
ACCAAGAGTGAGGCACCACT

Downstream 100 bases:

>100_bases
GCGGCGGAGTGCGCAGGCAACAAGTCAGTGAAGATGAAGCCGTCCCGCTCCACCGTCTCTCCCACGTACATCGCCAACGG
GCGCAGAAACATGGGACCAT

Product: two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLNLLDPDTKPTLLVVDDTPENLSLLSALLGPHYRVKVAISGQRALEILSSGTRVDLILLDVVMPQMDGYQVMQALQEMP
QARHIPVIFLTAKVEESAEAHGLALGAVDYIHKPISPAIVLARVRNHLELKAARDLLHDQNAYLEQEVERRTRENELIQN
ITIHSLASLAETRDNETGNHILRTQHYVKLLAETLCDHPRFRAELTPKTIDLLFKSAPLHDIGKVGVPDRILLKPGKLDA
DEFTQMKHHSILGKEAIVHAEEALGVEVEFLRLAKEIACYHHEKWDGTGYPYGLMGDEIPLSARLMALADVYDALISKRC
YKAAMSHTEVVPIILAGRSSHFDPDITDAFARIHLLFHEIALRYNDDKGEQEANLD

Sequences:

>Translated_376_residues
MLNLLDPDTKPTLLVVDDTPENLSLLSALLGPHYRVKVAISGQRALEILSSGTRVDLILLDVVMPQMDGYQVMQALQEMP
QARHIPVIFLTAKVEESAEAHGLALGAVDYIHKPISPAIVLARVRNHLELKAARDLLHDQNAYLEQEVERRTRENELIQN
ITIHSLASLAETRDNETGNHILRTQHYVKLLAETLCDHPRFRAELTPKTIDLLFKSAPLHDIGKVGVPDRILLKPGKLDA
DEFTQMKHHSILGKEAIVHAEEALGVEVEFLRLAKEIACYHHEKWDGTGYPYGLMGDEIPLSARLMALADVYDALISKRC
YKAAMSHTEVVPIILAGRSSHFDPDITDAFARIHLLFHEIALRYNDDKGEQEANLD
>Mature_376_residues
MLNLLDPDTKPTLLVVDDTPENLSLLSALLGPHYRVKVAISGQRALEILSSGTRVDLILLDVVMPQMDGYQVMQALQEMP
QARHIPVIFLTAKVEESAEAHGLALGAVDYIHKPISPAIVLARVRNHLELKAARDLLHDQNAYLEQEVERRTRENELIQN
ITIHSLASLAETRDNETGNHILRTQHYVKLLAETLCDHPRFRAELTPKTIDLLFKSAPLHDIGKVGVPDRILLKPGKLDA
DEFTQMKHHSILGKEAIVHAEEALGVEVEFLRLAKEIACYHHEKWDGTGYPYGLMGDEIPLSARLMALADVYDALISKRC
YKAAMSHTEVVPIILAGRSSHFDPDITDAFARIHLLFHEIALRYNDDKGEQEANLD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=136, Percent_Identity=36.0294117647059, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=37.1428571428571, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786599, Length=70, Percent_Identity=45.7142857142857, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42151; Mature: 42151

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLLDPDTKPTLLVVDDTPENLSLLSALLGPHYRVKVAISGQRALEILSSGTRVDLILL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCEEEEEEE
DVVMPQMDGYQVMQALQEMPQARHIPVIFLTAKVEESAEAHGLALGAVDYIHKPISPAIV
EHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHCCEEHHHHHHHHCCCCHHHH
LARVRNHLELKAARDLLHDQNAYLEQEVERRTRENELIQNITIHSLASLAETRDNETGNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ILRTQHYVKLLAETLCDHPRFRAELTPKTIDLLFKSAPLHDIGKVGVPDRILLKPGKLDA
HHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCH
DEFTQMKHHSILGKEAIVHAEEALGVEVEFLRLAKEIACYHHEKWDGTGYPYGLMGDEIP
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSARLMALADVYDALISKRCYKAAMSHTEVVPIILAGRSSHFDPDITDAFARIHLLFHEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ALRYNDDKGEQEANLD
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLNLLDPDTKPTLLVVDDTPENLSLLSALLGPHYRVKVAISGQRALEILSSGTRVDLILL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCEEEEEEE
DVVMPQMDGYQVMQALQEMPQARHIPVIFLTAKVEESAEAHGLALGAVDYIHKPISPAIV
EHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHCCEEHHHHHHHHCCCCHHHH
LARVRNHLELKAARDLLHDQNAYLEQEVERRTRENELIQNITIHSLASLAETRDNETGNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ILRTQHYVKLLAETLCDHPRFRAELTPKTIDLLFKSAPLHDIGKVGVPDRILLKPGKLDA
HHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCH
DEFTQMKHHSILGKEAIVHAEEALGVEVEFLRLAKEIACYHHEKWDGTGYPYGLMGDEIP
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSARLMALADVYDALISKRCYKAAMSHTEVVPIILAGRSSHFDPDITDAFARIHLLFHEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ALRYNDDKGEQEANLD
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]