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The map label for this gene is tyrS [H]

Identifier: 145300655

GI number: 145300655

Start: 4079794

End: 4081095

Strand: Direct

Name: tyrS [H]

Synonym: ASA_3787

Alternate gene names: 145300655

Gene position: 4079794-4081095 (Clockwise)

Preceding gene: 145300653

Following gene: 145300657

Centisome position: 86.76

GC content: 64.98

Gene sequence:

>1302_bases
ATGACGGCTTTTCCCCCTGCTGTTGGAGAACCCATGATCAACCCCCACCTGCTCGATGAGCTGAGCCAGCGCGGCCTTGT
GGCCCAGAATTCCGATCCGGCGGCCCTGGCCGACCACCTCGCCACGTCACGCACCGTCTATTGCGGGTTCGATCCCACCG
CCGGCAGCCTGCATATCGGTCATCTGGTGCCGCTCTTGATGCTGCGCCGCTTCCAGCTGGCAGGCCACACCCCGGTGGCA
CTGGTGGGCGGGGCGACCGGCCTTATCGGCGATCCCAGCTTCAAGGCGACCGAGCGCAGCCTCAACAGTGCCGATACCGT
GCTGGGTTGGGTGGCGAGCCTGTCGGCCCAGATCAGGGCGCTTCTGCCCGCCGATGACGGCCTGTCCGCCCCGCAACTGG
TCAACAATGGCGACTGGATGGGGCAGATGTCCGCCCTCGATTTTCTGCGGGATATCGGCAAGCACTTCTCGGTCAACGCC
ATGCTGGCACGGGAATCGGTGCGCCAGCGGCTGGCCCGCCCGGATCAGGGTATCTCGTTCACCGAGTTCTCCTACGCCCT
GCTGCAATCCTACGACTTTGCGGTGCTGCACCAGCGGCTCGGCTGCACCCTGCAGATTGGCGGCAACGACCAGTGGGGCA
ACATCACCAGCGGCATGGATCTCACCCGTCGCCTCCATCAGGCGCAGGTACACGGCATGACCCTGCCCCTTATCACTAAG
GCAGACGGCACCAAGTTCGGCAAGACCGAGGGTGGCGCGGTCTGGCTCGATCCGGCGCTCACCTCCCCTTACGCCTTCTA
CCAGTTCTGGCTCGGCACCGCGGATGAGGATGTCTACCGCTTCCTGCGCTACTACAGCTTTATGTCGCTGAGCGAGATTG
ATGCACTGGAGGAGGAAGATGCCCAGCGGCAGGGGCGCAAGCAGGCCCAGCAGGTGTTGGCAAACGAGCTGACCGAACTG
GTGCATGGCAAGGCGGCGCTGGCGGCGGTGCAGCGCATCAGTGAGCTGCTGTTTAGCGGCGACGTGGTCCGCCTCGGCGA
GAGCGATCTGGCCCAGCTGGCGCAGGATGGCATGCCGAGCAGCACAGTCAGCGGCGAGACTGATCTGGTCAGCCTGCTGG
TCAGCTGCGGTCTGGCGAACTCCAAACGCATCGCCCGCGAGCTGCTGGCGGCGGGGGCCATCAGCCTCAATGGCACCATC
AGGCAGGATGAGCAGCTCAGTGCCGAGGAGCGGCTGTTTGACCGCTACCTGCTGCTGCGCAGGGGCAAGAAGCAGTACCA
GCTGGTGGCGTGGTTGGGCTAG

Upstream 100 bases:

>100_bases
CGGTGGCGGGGAGCATCTCCCCACTATGGCTGGATGCTAGCACGAGAGTGACAAAACTGTCCCTCAGGGGACATATTCGC
CACGGGCCAGAACGGCGAGG

Downstream 100 bases:

>100_bases
CCTGCAGCGCCATAGCAACGAGAAAGGGCTGCCCGGGCAGCCCTTTCCATTGTGCGTCCCCACCTGTCAGCCGCTCGGTA
TACAGGGGAGGGCGGTGAAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTAFPPAVGEPMINPHLLDELSQRGLVAQNSDPAALADHLATSRTVYCGFDPTAGSLHIGHLVPLLMLRRFQLAGHTPVA
LVGGATGLIGDPSFKATERSLNSADTVLGWVASLSAQIRALLPADDGLSAPQLVNNGDWMGQMSALDFLRDIGKHFSVNA
MLARESVRQRLARPDQGISFTEFSYALLQSYDFAVLHQRLGCTLQIGGNDQWGNITSGMDLTRRLHQAQVHGMTLPLITK
ADGTKFGKTEGGAVWLDPALTSPYAFYQFWLGTADEDVYRFLRYYSFMSLSEIDALEEEDAQRQGRKQAQQVLANELTEL
VHGKAALAAVQRISELLFSGDVVRLGESDLAQLAQDGMPSSTVSGETDLVSLLVSCGLANSKRIARELLAAGAISLNGTI
RQDEQLSAEERLFDRYLLLRRGKKQYQLVAWLG

Sequences:

>Translated_433_residues
MTAFPPAVGEPMINPHLLDELSQRGLVAQNSDPAALADHLATSRTVYCGFDPTAGSLHIGHLVPLLMLRRFQLAGHTPVA
LVGGATGLIGDPSFKATERSLNSADTVLGWVASLSAQIRALLPADDGLSAPQLVNNGDWMGQMSALDFLRDIGKHFSVNA
MLARESVRQRLARPDQGISFTEFSYALLQSYDFAVLHQRLGCTLQIGGNDQWGNITSGMDLTRRLHQAQVHGMTLPLITK
ADGTKFGKTEGGAVWLDPALTSPYAFYQFWLGTADEDVYRFLRYYSFMSLSEIDALEEEDAQRQGRKQAQQVLANELTEL
VHGKAALAAVQRISELLFSGDVVRLGESDLAQLAQDGMPSSTVSGETDLVSLLVSCGLANSKRIARELLAAGAISLNGTI
RQDEQLSAEERLFDRYLLLRRGKKQYQLVAWLG
>Mature_432_residues
TAFPPAVGEPMINPHLLDELSQRGLVAQNSDPAALADHLATSRTVYCGFDPTAGSLHIGHLVPLLMLRRFQLAGHTPVAL
VGGATGLIGDPSFKATERSLNSADTVLGWVASLSAQIRALLPADDGLSAPQLVNNGDWMGQMSALDFLRDIGKHFSVNAM
LARESVRQRLARPDQGISFTEFSYALLQSYDFAVLHQRLGCTLQIGGNDQWGNITSGMDLTRRLHQAQVHGMTLPLITKA
DGTKFGKTEGGAVWLDPALTSPYAFYQFWLGTADEDVYRFLRYYSFMSLSEIDALEEEDAQRQGRKQAQQVLANELTELV
HGKAALAAVQRISELLFSGDVVRLGESDLAQLAQDGMPSSTVSGETDLVSLLVSCGLANSKRIARELLAAGAISLNGTIR
QDEQLSAEERLFDRYLLLRRGKKQYQLVAWLG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=36.6093366093366, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1787925, Length=423, Percent_Identity=59.8108747044917, Blast_Score=517, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI25145802, Length=404, Percent_Identity=35.6435643564356, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25145805, Length=318, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=38.302752293578, Blast_Score=280, Evalue=4e-76,
Organism=Drosophila melanogaster, GI20130383, Length=423, Percent_Identity=35.6973995271868, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47184; Mature: 47053

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFPPAVGEPMINPHLLDELSQRGLVAQNSDPAALADHLATSRTVYCGFDPTAGSLHIG
CCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHCCCEEEECCCCCCCCEEHH
HLVPLLMLRRFQLAGHTPVALVGGATGLIGDPSFKATERSLNSADTVLGWVASLSAQIRA
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHE
LLPADDGLSAPQLVNNGDWMGQMSALDFLRDIGKHFSVNAMLARESVRQRLARPDQGISF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCH
TEFSYALLQSYDFAVLHQRLGCTLQIGGNDQWGNITSGMDLTRRLHQAQVHGMTLPLITK
HHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
ADGTKFGKTEGGAVWLDPALTSPYAFYQFWLGTADEDVYRFLRYYSFMSLSEIDALEEED
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRQGRKQAQQVLANELTELVHGKAALAAVQRISELLFSGDVVRLGESDLAQLAQDGMPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCC
STVSGETDLVSLLVSCGLANSKRIARELLAAGAISLNGTIRQDEQLSAEERLFDRYLLLR
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCCCCCHHCCHHHHHHHHHHHHH
RGKKQYQLVAWLG
CCCCCEEEEEECC
>Mature Secondary Structure 
TAFPPAVGEPMINPHLLDELSQRGLVAQNSDPAALADHLATSRTVYCGFDPTAGSLHIG
CCCCCCCCCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHCCCEEEECCCCCCCCEEHH
HLVPLLMLRRFQLAGHTPVALVGGATGLIGDPSFKATERSLNSADTVLGWVASLSAQIRA
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHE
LLPADDGLSAPQLVNNGDWMGQMSALDFLRDIGKHFSVNAMLARESVRQRLARPDQGISF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCH
TEFSYALLQSYDFAVLHQRLGCTLQIGGNDQWGNITSGMDLTRRLHQAQVHGMTLPLITK
HHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
ADGTKFGKTEGGAVWLDPALTSPYAFYQFWLGTADEDVYRFLRYYSFMSLSEIDALEEED
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRQGRKQAQQVLANELTELVHGKAALAAVQRISELLFSGDVVRLGESDLAQLAQDGMPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCC
STVSGETDLVSLLVSCGLANSKRIARELLAAGAISLNGTIRQDEQLSAEERLFDRYLLLR
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCCCCCHHCCHHHHHHHHHHHHH
RGKKQYQLVAWLG
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA