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The map label for this gene is cpdB [H]

Identifier: 145300633

GI number: 145300633

Start: 4057547

End: 4059526

Strand: Direct

Name: cpdB [H]

Synonym: ASA_3765

Alternate gene names: 145300633

Gene position: 4057547-4059526 (Clockwise)

Preceding gene: 145300632

Following gene: 145300642

Centisome position: 86.29

GC content: 60.25

Gene sequence:

>1980_bases
ATGAAGCTGGGTGCTATTGCAGCCATAGTGCTGTTGTCTGCCTGTAGCAATGAAAACGACAGCAAGACGCAACCGGGCGC
TGTGGCCCTGCGTCTGATCCAGACCTCCGATATCCACTCCAACGTGCTGGGATACGATTACTACCAGAACAAGGAAGATC
GCAAGTTTGGCCTCTCCCGTACCGCCTTGCTGATCCGTGCGGCGCGCTCGGAAAACCCCAACAACCTGCTGCTCGACAAC
GGTGACCTGCTCCAGGGCACGCCGCTGGCGGATTACATCTTCGAGCAATCGGGCGCCGGTTACCTCGACAAGCAGGCCCA
CCCCGTGTTCAAGGCCATGAACGAGCTGGGTTATGACGCCGGAAACCTGGGCAACCACGAATTCAACTACGGGCTGGATT
ACCTCACGAAGACACTGCAGGGTGCCAAGTTCCCCTATGTGAACGCCAACGTGTTCGAGGCGGGCGCCTTCAAGCGCGAT
GCCAAGGGGCAGATCGACTGGAGTGGCAACAAGTTCACCCCTTACCTGCTGCTGGAGCGCAAGGTGAAGGATGACAAGGG
GCAGGAGCAGACCGTCAAGGTGGGCATATTGGGGCTGACCCCGCCGCAGGTGCTGCAGTGGGACAAGCGTCATCTGGAAG
GCAAGGTGGTGGTGGCCGACATGGTCGAGACCGCCAACCACTATGTGCCAGAGTTGCGTGCCAAGGGCGCCGACATAGTG
GTCGTGGTCGCCCACACCGGCATCAACGCCAGCAGCTACGAAGCCATGATGGAGAACTCCGCCTGGCACCTGGCCAAGGT
AAAAGGGGTGGATGCCCTCATGCTGGGCCATGCCCACAAGAACTTCCCCGGTGATTTCCCGGATCTGCCGGAGGTGGACA
ACCAGGCCGGTACCCTGAGCGGCGTGCCGACCGTGATGCCAGGCTATTGGGGCAACCATCTGGGCATCATAGACCTCACC
CTGGAGCAGGTTGAGGGCAAGTGGCAGGTGAAGCAAAGCCAGGCCAGCCTGCGCAAGATCGATTCCAGCGAGGGCAGCGC
CGTGGATCAGCGGGTCGTCGATCTGGTACAGGCGGATCACGATGCCACCAACCAGTGGCTGGATGAACCGCTCGGCAAAA
TCACCTCCTCCATCCACAGCTTCTTCGCTCTGGTACAGGACGACCCTTCGGTGCAACTGGTGAGCGATGCCCAGCGCCGC
TATGCCGAACAGCTGCAACAAGACGGTCTGCTCAAGGAGTCCTACCCCATCCTGGCGGTGGCGGCCCCCTTCCGTGGCGG
TCGCAACGGCATCAATGACTTCACTTATGTGGCCCAGGGCGACATCTCCCTGCGCAACGTCTCCGATCTCTATATCTATC
CGAACACCTTGCAGGTGGTCGAAGTGAACGGGGCTACCGTCAAGGAGTGGCTGGAGATGAGCGCCGGTCAGTTCAACCAG
ATCGACACCGGCAAGACAGAGGTGCAGTGGCTGGTGAACGAGAAGTTCCCCACCTACAACTTCGATGTGATCGATGGTGT
GAACTATGAAGTGGATATCACCCAGCCGGCCCGGTACAACAAGGAAGGGGACAAGGTCAGCAACGGCCAGCGCATCAGTG
CGCTCACCTTCAACGGCCAGCCCATTGACCCGGTCCAGAAGTTCTACGTGGTGACTAACAACTACCGCGCCAGCGGTGGC
GGCCACTTCCCGGGCATAGACGGCAGCAACATAGTGCATGAAGATCCCTCCGAGACGCGGGAGATCATTGCCCAGTACCT
GAAGGCCCAGTCTGCCGACAACCCGGGTGGTTTCAGCCCTGCGGCCAACAACAACTGGCACATGAAGGCCCTGCCGGCCA
ACGTGGACGTGCGGCTCTACTCCTCCCCCCGCGAAGAGGCCAAACAGCTGGCAGGCGCGGATCTGGAGTACAAGTCCACC
CTGCCGATGGACGATGCCAAACACCCCGGCTACGCCATATTCCGCCTGATCCGTCAGTAA

Upstream 100 bases:

>100_bases
AATTAATCCCGGCTTCATGCCATGACATATTCGTCTGTTAAATTCCCGTCGCTGGTAAAGCGACACTCATAATAAAAGGG
AAAAAACAGTGAATAAAACC

Downstream 100 bases:

>100_bases
TGCCTGACTGACACCGGCGATAAAAAAAGCCCTCGCAGTGCGAGGGCTTTTTCGTGGGCGGCTGGTGGCCTGTGCTTAGT
TCACCACGGCTACGTTTATG

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MKLGAIAAIVLLSACSNENDSKTQPGAVALRLIQTSDIHSNVLGYDYYQNKEDRKFGLSRTALLIRAARSENPNNLLLDN
GDLLQGTPLADYIFEQSGAGYLDKQAHPVFKAMNELGYDAGNLGNHEFNYGLDYLTKTLQGAKFPYVNANVFEAGAFKRD
AKGQIDWSGNKFTPYLLLERKVKDDKGQEQTVKVGILGLTPPQVLQWDKRHLEGKVVVADMVETANHYVPELRAKGADIV
VVVAHTGINASSYEAMMENSAWHLAKVKGVDALMLGHAHKNFPGDFPDLPEVDNQAGTLSGVPTVMPGYWGNHLGIIDLT
LEQVEGKWQVKQSQASLRKIDSSEGSAVDQRVVDLVQADHDATNQWLDEPLGKITSSIHSFFALVQDDPSVQLVSDAQRR
YAEQLQQDGLLKESYPILAVAAPFRGGRNGINDFTYVAQGDISLRNVSDLYIYPNTLQVVEVNGATVKEWLEMSAGQFNQ
IDTGKTEVQWLVNEKFPTYNFDVIDGVNYEVDITQPARYNKEGDKVSNGQRISALTFNGQPIDPVQKFYVVTNNYRASGG
GHFPGIDGSNIVHEDPSETREIIAQYLKAQSADNPGGFSPAANNNWHMKALPANVDVRLYSSPREEAKQLAGADLEYKST
LPMDDAKHPGYAIFRLIRQ

Sequences:

>Translated_659_residues
MKLGAIAAIVLLSACSNENDSKTQPGAVALRLIQTSDIHSNVLGYDYYQNKEDRKFGLSRTALLIRAARSENPNNLLLDN
GDLLQGTPLADYIFEQSGAGYLDKQAHPVFKAMNELGYDAGNLGNHEFNYGLDYLTKTLQGAKFPYVNANVFEAGAFKRD
AKGQIDWSGNKFTPYLLLERKVKDDKGQEQTVKVGILGLTPPQVLQWDKRHLEGKVVVADMVETANHYVPELRAKGADIV
VVVAHTGINASSYEAMMENSAWHLAKVKGVDALMLGHAHKNFPGDFPDLPEVDNQAGTLSGVPTVMPGYWGNHLGIIDLT
LEQVEGKWQVKQSQASLRKIDSSEGSAVDQRVVDLVQADHDATNQWLDEPLGKITSSIHSFFALVQDDPSVQLVSDAQRR
YAEQLQQDGLLKESYPILAVAAPFRGGRNGINDFTYVAQGDISLRNVSDLYIYPNTLQVVEVNGATVKEWLEMSAGQFNQ
IDTGKTEVQWLVNEKFPTYNFDVIDGVNYEVDITQPARYNKEGDKVSNGQRISALTFNGQPIDPVQKFYVVTNNYRASGG
GHFPGIDGSNIVHEDPSETREIIAQYLKAQSADNPGGFSPAANNNWHMKALPANVDVRLYSSPREEAKQLAGADLEYKST
LPMDDAKHPGYAIFRLIRQ
>Mature_659_residues
MKLGAIAAIVLLSACSNENDSKTQPGAVALRLIQTSDIHSNVLGYDYYQNKEDRKFGLSRTALLIRAARSENPNNLLLDN
GDLLQGTPLADYIFEQSGAGYLDKQAHPVFKAMNELGYDAGNLGNHEFNYGLDYLTKTLQGAKFPYVNANVFEAGAFKRD
AKGQIDWSGNKFTPYLLLERKVKDDKGQEQTVKVGILGLTPPQVLQWDKRHLEGKVVVADMVETANHYVPELRAKGADIV
VVVAHTGINASSYEAMMENSAWHLAKVKGVDALMLGHAHKNFPGDFPDLPEVDNQAGTLSGVPTVMPGYWGNHLGIIDLT
LEQVEGKWQVKQSQASLRKIDSSEGSAVDQRVVDLVQADHDATNQWLDEPLGKITSSIHSFFALVQDDPSVQLVSDAQRR
YAEQLQQDGLLKESYPILAVAAPFRGGRNGINDFTYVAQGDISLRNVSDLYIYPNTLQVVEVNGATVKEWLEMSAGQFNQ
IDTGKTEVQWLVNEKFPTYNFDVIDGVNYEVDITQPARYNKEGDKVSNGQRISALTFNGQPIDPVQKFYVVTNNYRASGG
GHFPGIDGSNIVHEDPSETREIIAQYLKAQSADNPGGFSPAANNNWHMKALPANVDVRLYSSPREEAKQLAGADLEYKST
LPMDDAKHPGYAIFRLIRQ

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=669, Percent_Identity=46.9357249626308, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1786687, Length=645, Percent_Identity=20.9302325581395, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72718; Mature: 72718

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGAIAAIVLLSACSNENDSKTQPGAVALRLIQTSDIHSNVLGYDYYQNKEDRKFGLSR
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCHHHHCCHH
TALLIRAARSENPNNLLLDNGDLLQGTPLADYIFEQSGAGYLDKQAHPVFKAMNELGYDA
HHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
GNLGNHEFNYGLDYLTKTLQGAKFPYVNANVFEAGAFKRDAKGQIDWSGNKFTPYLLLER
CCCCCCCCCCCHHHHHHHHCCCCCCEECCCEEECCCCCCCCCCEEECCCCCCCCEEEEEE
KVKDDKGQEQTVKVGILGLTPPQVLQWDKRHLEGKVVVADMVETANHYVPELRAKGADIV
HHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEE
VVVAHTGINASSYEAMMENSAWHLAKVKGVDALMLGHAHKNFPGDFPDLPEVDNQAGTLS
EEEEECCCCHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GVPTVMPGYWGNHLGIIDLTLEQVEGKWQVKQSQASLRKIDSSEGSAVDQRVVDLVQADH
CCCCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
DATNQWLDEPLGKITSSIHSFFALVQDDPSVQLVSDAQRRYAEQLQQDGLLKESYPILAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AAPFRGGRNGINDFTYVAQGDISLRNVSDLYIYPNTLQVVEVNGATVKEWLEMSAGQFNQ
ECCCCCCCCCCCCCEEEEECCEEEECCEEEEEECCEEEEEEECCCHHHHHHHHCCCCCCC
IDTGKTEVQWLVNEKFPTYNFDVIDGVNYEVDITQPARYNKEGDKVSNGQRISALTFNGQ
CCCCCCEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCC
PIDPVQKFYVVTNNYRASGGGHFPGIDGSNIVHEDPSETREIIAQYLKAQSADNPGGFSP
CCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AANNNWHMKALPANVDVRLYSSPREEAKQLAGADLEYKSTLPMDDAKHPGYAIFRLIRQ
CCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKLGAIAAIVLLSACSNENDSKTQPGAVALRLIQTSDIHSNVLGYDYYQNKEDRKFGLSR
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCHHHHCCHH
TALLIRAARSENPNNLLLDNGDLLQGTPLADYIFEQSGAGYLDKQAHPVFKAMNELGYDA
HHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
GNLGNHEFNYGLDYLTKTLQGAKFPYVNANVFEAGAFKRDAKGQIDWSGNKFTPYLLLER
CCCCCCCCCCCHHHHHHHHCCCCCCEECCCEEECCCCCCCCCCEEECCCCCCCCEEEEEE
KVKDDKGQEQTVKVGILGLTPPQVLQWDKRHLEGKVVVADMVETANHYVPELRAKGADIV
HHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEE
VVVAHTGINASSYEAMMENSAWHLAKVKGVDALMLGHAHKNFPGDFPDLPEVDNQAGTLS
EEEEECCCCHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GVPTVMPGYWGNHLGIIDLTLEQVEGKWQVKQSQASLRKIDSSEGSAVDQRVVDLVQADH
CCCCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
DATNQWLDEPLGKITSSIHSFFALVQDDPSVQLVSDAQRRYAEQLQQDGLLKESYPILAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AAPFRGGRNGINDFTYVAQGDISLRNVSDLYIYPNTLQVVEVNGATVKEWLEMSAGQFNQ
ECCCCCCCCCCCCCEEEEECCEEEECCEEEEEECCEEEEEEECCCHHHHHHHHCCCCCCC
IDTGKTEVQWLVNEKFPTYNFDVIDGVNYEVDITQPARYNKEGDKVSNGQRISALTFNGQ
CCCCCCEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCC
PIDPVQKFYVVTNNYRASGGGHFPGIDGSNIVHEDPSETREIIAQYLKAQSADNPGGFSP
CCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AANNNWHMKALPANVDVRLYSSPREEAKQLAGADLEYKSTLPMDDAKHPGYAIFRLIRQ
CCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]