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The map label for this gene is paaF [H]

Identifier: 145300489

GI number: 145300489

Start: 3913165

End: 3913914

Strand: Direct

Name: paaF [H]

Synonym: ASA_3621

Alternate gene names: 145300489

Gene position: 3913165-3913914 (Clockwise)

Preceding gene: 145300485

Following gene: 145300491

Centisome position: 83.22

GC content: 61.87

Gene sequence:

>750_bases
ATGGACCCCATTATCGTTGCCGAGCAGCAGGATGGCTTGCTCACCCTGACCTTCAATCGCCCCGACAAGCGCAATGCCCT
CAATACCGACATGTATCGTGCGCTCACTCAGGCACTGCGGCAAGCGACACAAGATGACGCCGTACACGCGTTGCTGATAC
AGGGACAAAGCGATTGCTTCACCGCCGGCAATGACCTGGCCGATTTTATGGGCAAATCCAGCCTGGATGCCGATGACCCC
ATACTGCAGTTCCTGCACACACTGGCGGATTTTCCCAAGCCCGTCATCGCCGCGGTCGGGGGGCCGGCAGTCGGGATCGG
CACCACCTTGCTGCTGCACTGCGATCTTGTCTATCTGGGGGATAATGCCCGGCTGCAACTGCCGTTCGTCGAACTGGGGC
TGGTGCCGGAGTTTGCCTCCAGCCTGCTGCTGCCCCGCGCCGTGGGTCATCTGAAGGCGGCGGAACTCTTGCTGCTGGCC
GAGGCCATCGATGCTGATGAAGCACTGCGCCTGGGTCTGGCCAACCGGGTAGTTGCCGCCGATGAGCTGCTGGCACTGGC
TCGGGATCAGGGATTGAAGCTGGCGGCCAAGCCGCCCAAGGCGCTGCAAAAGAGCAAGGCACTGCTCAAACAGGAGCTGA
AACAGGCGGTGCACTTCGTCATCGATCTGGAGGCGCGCGCCTTCTCCCTCGCCTTGCAGGGGGAAGAGGCGCAACACAGG
ATCGCGCAAAGGCTCAAACCCAGGGCCTGA

Upstream 100 bases:

>100_bases
CGTTTAAACATGGTTGAAATCCTTTTATGATGTTGTTAAACGCCGTGAAGCTTACCGCAGCAGTAATCTTTTGGCAGCCT
GTGATAACAAGGATTTAGAC

Downstream 100 bases:

>100_bases
TCCTCAGTTGAGACGAAACCGGCCTATCAGCTCGCAGCCCTGTACCTTGTCCTGCAGCTGGATCAGTCGGGCGATCTCGG
GATTGGGGTGGCGCTTGAGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MDPIIVAEQQDGLLTLTFNRPDKRNALNTDMYRALTQALRQATQDDAVHALLIQGQSDCFTAGNDLADFMGKSSLDADDP
ILQFLHTLADFPKPVIAAVGGPAVGIGTTLLLHCDLVYLGDNARLQLPFVELGLVPEFASSLLLPRAVGHLKAAELLLLA
EAIDADEALRLGLANRVVAADELLALARDQGLKLAAKPPKALQKSKALLKQELKQAVHFVIDLEARAFSLALQGEEAQHR
IAQRLKPRA

Sequences:

>Translated_249_residues
MDPIIVAEQQDGLLTLTFNRPDKRNALNTDMYRALTQALRQATQDDAVHALLIQGQSDCFTAGNDLADFMGKSSLDADDP
ILQFLHTLADFPKPVIAAVGGPAVGIGTTLLLHCDLVYLGDNARLQLPFVELGLVPEFASSLLLPRAVGHLKAAELLLLA
EAIDADEALRLGLANRVVAADELLALARDQGLKLAAKPPKALQKSKALLKQELKQAVHFVIDLEARAFSLALQGEEAQHR
IAQRLKPRA
>Mature_249_residues
MDPIIVAEQQDGLLTLTFNRPDKRNALNTDMYRALTQALRQATQDDAVHALLIQGQSDCFTAGNDLADFMGKSSLDADDP
ILQFLHTLADFPKPVIAAVGGPAVGIGTTLLLHCDLVYLGDNARLQLPFVELGLVPEFASSLLLPRAVGHLKAAELLLLA
EAIDADEALRLGLANRVVAADELLALARDQGLKLAAKPPKALQKSKALLKQELKQAVHFVIDLEARAFSLALQGEEAQHR
IAQRLKPRA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=224, Percent_Identity=37.5, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI45643119, Length=224, Percent_Identity=37.5, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI260275230, Length=224, Percent_Identity=37.5, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=30.9236947791165, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=30.9236947791165, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=31.0204081632653, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=31.0204081632653, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI4757968, Length=245, Percent_Identity=30.6122448979592, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI49355787, Length=245, Percent_Identity=30.6122448979592, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI221136756, Length=252, Percent_Identity=29.7619047619048, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI221136753, Length=252, Percent_Identity=29.7619047619048, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI221307494, Length=252, Percent_Identity=29.7619047619048, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI70995211, Length=220, Percent_Identity=32.2727272727273, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI194097323, Length=198, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI116235487, Length=229, Percent_Identity=27.0742358078603, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=28.4, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI68989263, Length=226, Percent_Identity=31.858407079646, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4502327, Length=179, Percent_Identity=29.608938547486, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=36.8, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787660, Length=234, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=31.7204301075269, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.7937743190661, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI221142681, Length=213, Percent_Identity=30.9859154929577, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082183, Length=204, Percent_Identity=27.9411764705882, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=30.3191489361702, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=240, Percent_Identity=32.5, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=29.4820717131474, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=32.0574162679426, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=214, Percent_Identity=29.4392523364486, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=30.1435406698565, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=27.4336283185841, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=167, Percent_Identity=32.3353293413174, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=218, Percent_Identity=26.1467889908257, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=225, Percent_Identity=25.3333333333333, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=205, Percent_Identity=26.8292682926829, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=211, Percent_Identity=33.6492890995261, Blast_Score=133, Evalue=8e-32,
Organism=Drosophila melanogaster, GI20129971, Length=224, Percent_Identity=33.4821428571429, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653477, Length=224, Percent_Identity=33.4821428571429, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653139, Length=190, Percent_Identity=29.4736842105263, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=27.2277227722772, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=26.8722466960352, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922422, Length=184, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21357171, Length=203, Percent_Identity=26.6009852216749, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26885; Mature: 26885

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIIVAEQQDGLLTLTFNRPDKRNALNTDMYRALTQALRQATQDDAVHALLIQGQSDCF
CCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TAGNDLADFMGKSSLDADDPILQFLHTLADFPKPVIAAVGGPAVGIGTTLLLHCDLVYLG
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHEEEEEEC
DNARLQLPFVELGLVPEFASSLLLPRAVGHLKAAELLLLAEAIDADEALRLGLANRVVAA
CCCEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DELLALARDQGLKLAAKPPKALQKSKALLKQELKQAVHFVIDLEARAFSLALQGEEAQHR
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCHHHHH
IAQRLKPRA
HHHHCCCCC
>Mature Secondary Structure
MDPIIVAEQQDGLLTLTFNRPDKRNALNTDMYRALTQALRQATQDDAVHALLIQGQSDCF
CCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TAGNDLADFMGKSSLDADDPILQFLHTLADFPKPVIAAVGGPAVGIGTTLLLHCDLVYLG
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHEEEEEEC
DNARLQLPFVELGLVPEFASSLLLPRAVGHLKAAELLLLAEAIDADEALRLGLANRVVAA
CCCEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DELLALARDQGLKLAAKPPKALQKSKALLKQELKQAVHFVIDLEARAFSLALQGEEAQHR
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCHHHHH
IAQRLKPRA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]