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The map label for this gene is amyA [H]

Identifier: 145300331

GI number: 145300331

Start: 3743331

End: 3745595

Strand: Direct

Name: amyA [H]

Synonym: ASA_3455

Alternate gene names: 145300331

Gene position: 3743331-3745595 (Clockwise)

Preceding gene: 145300330

Following gene: 145300332

Centisome position: 79.6

GC content: 58.54

Gene sequence:

>2265_bases
ATGCACAACACCCTGTTTCAAACTGCCATGCTCACTGCGGTCCTGAGCTCATTCAGCCACACTGCCACCGCCGAAGGCGT
CATGGTTCACCTGTTCCAATGGAAGTTCAACGACATCGCCAACGAGTGCGAGAATGTCCTCGGCCCCAAGGGATTCGGAA
GCGTTCAGATCACGCCGCCTGCCGAGCACAAGCAAGGAAGCCAGGTCTGGTGGACCGTCTATCAACCTGTCAGCTTCAAA
AATTTCAACAGCCATGGCGGCAACGAGGCGGAGCTCAAGAGCATGATCGCCCGCTGTAACGCCGCCGGGGTCAAGATCTA
CGCCGATGCCGTCTTCAACCAGCTGGCATCGGGCACGGGAACCGCCACCGGCGGCGGCAGCTACAACTCGGGGCAATATC
AGTATCCCCAGTTCGGCTACAACGACTTCCACCATAGCGGTGACATCACCAACTACGGCGACAGCAACAACGTCTGGAAT
GGCGCGCTCTACGGGATGCCGGACCTGAAAACCGAGTCGCCCTATGTGCAGGATCAGATTGCCACCTATATGAAGACCCT
GCTCGGTTGGGGAGTTGCGGGTTTTCGGGTTGACGCGGCCAAACACATGGCCCCTGCCGATGTGAAGGCCATCCTCGTCA
AGGCAGGCTCCCCAAAAGCCTATCTGGAAGTGATCGGTGCAGGTGGTGAATCCGCCGAGATCCAGCCCAATCGCTACACC
CATATAGACACAGTCACCGAGTTCAAGTACGGCACCGATCTCGCGGCCAACTTCAATGGCAAGATCAAGAATCTCAAGAC
CCTGGGAGAGAGCTGGGGCCTGCTCCCTTCCGACAAGTCCTTCATCTTCGTGGTCAACCACGACCGCGAGCGCGGTCACG
GTGGCGGCGGCATGCTCACTTTCATGAACGGTGCCCGCTATGAGCTGGCCAACGTCTTCATGATGGCCTGGCCCTATGGC
TGGAAGCAGGTAATGTCGGGATATCGCTTCGAGAACATGGGTACCTACGAAACCGACAAGGGCGCCCCCGGCAGCACACC
TTGCTCCGATACCCAGTGGAACTGTGAACAACGCCGTCCACAGATCATGAACATGGCCATGTTCCACAACCAGACCAAGG
ATCTGCCGGTCAACAACTGGTGGGATAACGGCAACAACCAGATTGCCTTCAGTCGGGGTAACAAAGGTTTCGTCGCCATC
AACAACGAAAGCGGCAACCTGGTGGCCTCAGTCCAGACCGGACTGCCAGCTGGCGAGTACTGCAACATCCTGAGTGGCAA
TGATTACTGCAGCGGCGCCTACATCAAGGTCGATGGCAGCGGCAAGGCTGGCCTGAACCTCGCCGGCATGAAGGCCGCCG
CCATCCTGGCCAGCTGTACCAAAGCAGCCCCTTGCGGTGGCGTCATACCGGGTAACCGATTCAGCAGCCTGAACCTGCGC
GGTACTCATAATGCCTGGGGCAACACGGCAATGCAGGTGGATGACAACCGCATCTGGTCGGCCATCATCAACTTCACCGG
CGCCGGTGACACCAGCGGTGCCCAGCGCTTCAAGTTCGATGTGTTTGGCAACTGGACCGAGAACTACGGTGATAACGAAG
GGGACGGCATTGCCGACAAGGGCCGCACCAGGGATATCTACTTCAATGGTGCAGGCCAGTATCGTATCGCCCTCAAGGAG
AGCGATCTCAGCTACACAGTCACTGCATTGAGCAATAACCAGGCGCCAGTTGCGGTCATCTCGCCCAAAAAACCCAGCGT
CAAGCTGGGTGAGAGCCTGGTGTTTGACGCCTCAGGCTCCACCGATGATGCCGGTGTTGCGAGTTACAGCTGGTCCACCG
GTGGCATCGGCAAGACAGAGACTGTCCAGTTCGATACCCTGGGTTCCCGCACAGTGACAGTCACTGTCACCGATACAGAA
GGGCTAACAGCCAGTGTCAGCGCGACCGTGCACGTGACCGACGGCAGTGGCGCCTACACCAGCGAGCTGCCGACCCTGCA
CTTTCGCGGTACCCCGAACGGCTGGGGTGTTCTTGCCATGACCCTGGTCGCCGACAATCAGTGGGAAGCACGAGTCACCT
TCGATGGCCAGACCAACCAGCGCTTCAAGTTCGATGTAAAGGGTGACTGGAGCCAGAACTACGGGGATACCAACAAGGAT
GGCGTCGCCGAGCTGACCGGTGCCGACATCACTACCCCGGTGGTGGGAGCCTATGTGGTCCGCTTCAACGATCAAACCCT
GACCTACAGCCTGAACGCCCAGTAA

Upstream 100 bases:

>100_bases
ATTTCATTGATGCTACTGAGCCGAACGAGGATTGTTCGTTGTCCGTTCGAAATTCCGACAGTGATACATCAATAACAGCA
AAATCGAAGGGAAAATCGTC

Downstream 100 bases:

>100_bases
ACCCCAAGGGGTTGCGACGCTTTCAGGTTGCTACCAACCAGCAGGGCCTCCTTCCCATCCGGGCAGGAGGCCCTGCTCAT
GTGGGGCCATCTCAACTGCC

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MHNTLFQTAMLTAVLSSFSHTATAEGVMVHLFQWKFNDIANECENVLGPKGFGSVQITPPAEHKQGSQVWWTVYQPVSFK
NFNSHGGNEAELKSMIARCNAAGVKIYADAVFNQLASGTGTATGGGSYNSGQYQYPQFGYNDFHHSGDITNYGDSNNVWN
GALYGMPDLKTESPYVQDQIATYMKTLLGWGVAGFRVDAAKHMAPADVKAILVKAGSPKAYLEVIGAGGESAEIQPNRYT
HIDTVTEFKYGTDLAANFNGKIKNLKTLGESWGLLPSDKSFIFVVNHDRERGHGGGGMLTFMNGARYELANVFMMAWPYG
WKQVMSGYRFENMGTYETDKGAPGSTPCSDTQWNCEQRRPQIMNMAMFHNQTKDLPVNNWWDNGNNQIAFSRGNKGFVAI
NNESGNLVASVQTGLPAGEYCNILSGNDYCSGAYIKVDGSGKAGLNLAGMKAAAILASCTKAAPCGGVIPGNRFSSLNLR
GTHNAWGNTAMQVDDNRIWSAIINFTGAGDTSGAQRFKFDVFGNWTENYGDNEGDGIADKGRTRDIYFNGAGQYRIALKE
SDLSYTVTALSNNQAPVAVISPKKPSVKLGESLVFDASGSTDDAGVASYSWSTGGIGKTETVQFDTLGSRTVTVTVTDTE
GLTASVSATVHVTDGSGAYTSELPTLHFRGTPNGWGVLAMTLVADNQWEARVTFDGQTNQRFKFDVKGDWSQNYGDTNKD
GVAELTGADITTPVVGAYVVRFNDQTLTYSLNAQ

Sequences:

>Translated_754_residues
MHNTLFQTAMLTAVLSSFSHTATAEGVMVHLFQWKFNDIANECENVLGPKGFGSVQITPPAEHKQGSQVWWTVYQPVSFK
NFNSHGGNEAELKSMIARCNAAGVKIYADAVFNQLASGTGTATGGGSYNSGQYQYPQFGYNDFHHSGDITNYGDSNNVWN
GALYGMPDLKTESPYVQDQIATYMKTLLGWGVAGFRVDAAKHMAPADVKAILVKAGSPKAYLEVIGAGGESAEIQPNRYT
HIDTVTEFKYGTDLAANFNGKIKNLKTLGESWGLLPSDKSFIFVVNHDRERGHGGGGMLTFMNGARYELANVFMMAWPYG
WKQVMSGYRFENMGTYETDKGAPGSTPCSDTQWNCEQRRPQIMNMAMFHNQTKDLPVNNWWDNGNNQIAFSRGNKGFVAI
NNESGNLVASVQTGLPAGEYCNILSGNDYCSGAYIKVDGSGKAGLNLAGMKAAAILASCTKAAPCGGVIPGNRFSSLNLR
GTHNAWGNTAMQVDDNRIWSAIINFTGAGDTSGAQRFKFDVFGNWTENYGDNEGDGIADKGRTRDIYFNGAGQYRIALKE
SDLSYTVTALSNNQAPVAVISPKKPSVKLGESLVFDASGSTDDAGVASYSWSTGGIGKTETVQFDTLGSRTVTVTVTDTE
GLTASVSATVHVTDGSGAYTSELPTLHFRGTPNGWGVLAMTLVADNQWEARVTFDGQTNQRFKFDVKGDWSQNYGDTNKD
GVAELTGADITTPVVGAYVVRFNDQTLTYSLNAQ
>Mature_754_residues
MHNTLFQTAMLTAVLSSFSHTATAEGVMVHLFQWKFNDIANECENVLGPKGFGSVQITPPAEHKQGSQVWWTVYQPVSFK
NFNSHGGNEAELKSMIARCNAAGVKIYADAVFNQLASGTGTATGGGSYNSGQYQYPQFGYNDFHHSGDITNYGDSNNVWN
GALYGMPDLKTESPYVQDQIATYMKTLLGWGVAGFRVDAAKHMAPADVKAILVKAGSPKAYLEVIGAGGESAEIQPNRYT
HIDTVTEFKYGTDLAANFNGKIKNLKTLGESWGLLPSDKSFIFVVNHDRERGHGGGGMLTFMNGARYELANVFMMAWPYG
WKQVMSGYRFENMGTYETDKGAPGSTPCSDTQWNCEQRRPQIMNMAMFHNQTKDLPVNNWWDNGNNQIAFSRGNKGFVAI
NNESGNLVASVQTGLPAGEYCNILSGNDYCSGAYIKVDGSGKAGLNLAGMKAAAILASCTKAAPCGGVIPGNRFSSLNLR
GTHNAWGNTAMQVDDNRIWSAIINFTGAGDTSGAQRFKFDVFGNWTENYGDNEGDGIADKGRTRDIYFNGAGQYRIALKE
SDLSYTVTALSNNQAPVAVISPKKPSVKLGESLVFDASGSTDDAGVASYSWSTGGIGKTETVQFDTLGSRTVTVTVTDTE
GLTASVSATVHVTDGSGAYTSELPTLHFRGTPNGWGVLAMTLVADNQWEARVTFDGQTNQRFKFDVKGDWSQNYGDTNKD
GVAELTGADITTPVVGAYVVRFNDQTLTYSLNAQ

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI4502085, Length=481, Percent_Identity=43.4511434511435, Blast_Score=345, Evalue=1e-94,
Organism=Homo sapiens, GI10280622, Length=481, Percent_Identity=43.4511434511435, Blast_Score=344, Evalue=2e-94,
Organism=Homo sapiens, GI56549664, Length=504, Percent_Identity=42.0634920634921, Blast_Score=343, Evalue=5e-94,
Organism=Homo sapiens, GI56549662, Length=504, Percent_Identity=42.0634920634921, Blast_Score=343, Evalue=5e-94,
Organism=Homo sapiens, GI40254482, Length=504, Percent_Identity=42.0634920634921, Blast_Score=343, Evalue=5e-94,
Organism=Homo sapiens, GI56549660, Length=504, Percent_Identity=42.0634920634921, Blast_Score=343, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17558728, Length=505, Percent_Identity=33.8613861386139, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI18859631, Length=493, Percent_Identity=40.3651115618661, Blast_Score=342, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24654362, Length=471, Percent_Identity=41.4012738853503, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI17137384, Length=486, Percent_Identity=35.59670781893, Blast_Score=296, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006046
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 81392; Mature: 81392

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50093 PKD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNTLFQTAMLTAVLSSFSHTATAEGVMVHLFQWKFNDIANECENVLGPKGFGSVQITPP
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCEEECCC
AEHKQGSQVWWTVYQPVSFKNFNSHGGNEAELKSMIARCNAAGVKIYADAVFNQLASGTG
CCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCC
TATGGGSYNSGQYQYPQFGYNDFHHSGDITNYGDSNNVWNGALYGMPDLKTESPYVQDQI
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
ATYMKTLLGWGVAGFRVDAAKHMAPADVKAILVKAGSPKAYLEVIGAGGESAEIQPNRYT
HHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEECCCCEE
HIDTVTEFKYGTDLAANFNGKIKNLKTLGESWGLLPSDKSFIFVVNHDRERGHGGGGMLT
EECEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEE
FMNGARYELANVFMMAWPYGWKQVMSGYRFENMGTYETDKGAPGSTPCSDTQWNCEQRRP
EECCCCEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
QIMNMAMFHNQTKDLPVNNWWDNGNNQIAFSRGNKGFVAINNESGNLVASVQTGLPAGEY
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCHH
CNILSGNDYCSGAYIKVDGSGKAGLNLAGMKAAAILASCTKAAPCGGVIPGNRFSSLNLR
EEEECCCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEC
GTHNAWGNTAMQVDDNRIWSAIINFTGAGDTSGAQRFKFDVFGNWTENYGDNEGDGIADK
CCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHCCCCCCCCCCCCC
GRTRDIYFNGAGQYRIALKESDLSYTVTALSNNQAPVAVISPKKPSVKLGESLVFDASGS
CCEEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCC
TDDAGVASYSWSTGGIGKTETVQFDTLGSRTVTVTVTDTEGLTASVSATVHVTDGSGAYT
CCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCC
SELPTLHFRGTPNGWGVLAMTLVADNQWEARVTFDGQTNQRFKFDVKGDWSQNYGDTNKD
CCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCC
GVAELTGADITTPVVGAYVVRFNDQTLTYSLNAQ
CCEEECCCCCCCCCEEEEEEEECCCEEEEEECCC
>Mature Secondary Structure
MHNTLFQTAMLTAVLSSFSHTATAEGVMVHLFQWKFNDIANECENVLGPKGFGSVQITPP
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCEEECCC
AEHKQGSQVWWTVYQPVSFKNFNSHGGNEAELKSMIARCNAAGVKIYADAVFNQLASGTG
CCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCC
TATGGGSYNSGQYQYPQFGYNDFHHSGDITNYGDSNNVWNGALYGMPDLKTESPYVQDQI
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
ATYMKTLLGWGVAGFRVDAAKHMAPADVKAILVKAGSPKAYLEVIGAGGESAEIQPNRYT
HHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEECCCCEE
HIDTVTEFKYGTDLAANFNGKIKNLKTLGESWGLLPSDKSFIFVVNHDRERGHGGGGMLT
EECEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEE
FMNGARYELANVFMMAWPYGWKQVMSGYRFENMGTYETDKGAPGSTPCSDTQWNCEQRRP
EECCCCEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
QIMNMAMFHNQTKDLPVNNWWDNGNNQIAFSRGNKGFVAINNESGNLVASVQTGLPAGEY
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCHH
CNILSGNDYCSGAYIKVDGSGKAGLNLAGMKAAAILASCTKAAPCGGVIPGNRFSSLNLR
EEEECCCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEC
GTHNAWGNTAMQVDDNRIWSAIINFTGAGDTSGAQRFKFDVFGNWTENYGDNEGDGIADK
CCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHCCCCCCCCCCCCC
GRTRDIYFNGAGQYRIALKESDLSYTVTALSNNQAPVAVISPKKPSVKLGESLVFDASGS
CCEEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCC
TDDAGVASYSWSTGGIGKTETVQFDTLGSRTVTVTVTDTEGLTASVSATVHVTDGSGAYT
CCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCC
SELPTLHFRGTPNGWGVLAMTLVADNQWEARVTFDGQTNQRFKFDVKGDWSQNYGDTNKD
CCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCC
GVAELTGADITTPVVGAYVVRFNDQTLTYSLNAQ
CCEEECCCCCCCCCEEEEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8126440 [H]