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The map label for this gene is ackA [H]

Identifier: 145300283

GI number: 145300283

Start: 3680158

End: 3681360

Strand: Direct

Name: ackA [H]

Synonym: ASA_3401

Alternate gene names: 145300283

Gene position: 3680158-3681360 (Clockwise)

Preceding gene: 145300281

Following gene: 145300284

Centisome position: 78.26

GC content: 58.85

Gene sequence:

>1203_bases
ATGAGTAAGCTGGTTCTTGTACTTAACTGTGGCAGCTCGTCCCTGAAGTTTGCTGTCATTGACGCAACCACCGGTGATGA
CCTGCTGTCCGGCTTGGCCGAGTGTTTCAACCTCGACGACGCCCGCATCAAGTGGAAACTGAATGGCGAGAAAGGCAGTG
CCGATCTGGGCGCGGGTGCCGCTCACCAGGAAGCGCTGGACTACATCGTCAACAAGATCCTGCCGCTCAATCCGGAACTG
TCCCAGAATCTGATCGCCATTGGTCACCGTGTTGTTCACGGTGGTGAGCGCTTCACCTCCTCCGTTGTGATCTGTGATGA
CGTCATCGCCGGCATCGAAGCCGCCGTACCTTATGCACCGCTGCACAACCCGGCTCACCTGATCGGTATCCGTGAAGCCC
GTCGTATCTTCCCGGCCCTGGCCGACAAGAACGTGGCCGTGTTCGACACCGCCTTCCACCAGACCATGCCGGAAGAAGCC
TTCCTGTACGCCCTGCCGTACAAACTCTACAAAGAGAACGGCATCCGTCGCTACGGCGCCCACGGCACCAGCCACTACTA
CATCAGCCTGGAAGCCGCCAAGCAGCTGAACAAGCCGGTTGACGAGCTGAACATCATCAACTGCCACTTGGGCAACGGCG
GTTCCGTCTGCGCCATCAAGAACGGCAAATCCGTTGATACCTCCATGGGTCTGACCCCGCTGGAAGGCCTGGTCATGGGT
ACCCGCTCCGGCGATCTGGATCCGGCCATCATCTTCTTCCTGCACGACAAGCTGGGCATGAGCGTGGCGGATATCAACAC
CATGCTGACCAAAGAGTCCGGCCTGCAGGGTCTGACCGAAGTGACTTCCGACTGCCGTTTCGTTGAAGACAACTACGACA
GCAAAGAAGAAGCCAAGCGTGCCATGGATGTTTACTGCTATCGCCTGGCCAAGTACATCGGCGCCTACGCCGCCGCCATG
GACGGTCGTCTGGATGCCGTGGTCTTCACCGGTGGTATCGGTGAAAACTCAGCTCCGATCCGTGAAATCACCCTGAACAA
GCTGGGTCTGCTGGGCTTCGACGTCGATCACGACGCCAACCTGGCTGCCCGCTTCGGCAAGGGTGGCGAGATCACCAAGG
CTGGTTCTACCAAGGCCCTGGTTATCCCGACCAACGAGGAGTGGGTCATTGCCCACGACGCGCTGGAACTGGTCGGCGCA
TAA

Upstream 100 bases:

>100_bases
CAACAGATTGCCAACCCTCATAAGGATAAGTTGAAAGTTTTTGGACGCAACGCGTCCAACGCATCCTCCTACACAACCCA
ATCTGCGATAGGATTTATTC

Downstream 100 bases:

>100_bases
ATTCGCTGTAGAGCAAGGCCGCCCCAGGGCGGCCTTTTCCTGATCCCAAAAAATGAACAAGGGGTATTAAGTGGCACGCA
CTATTATGCTTATCCCGGTC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSKLVLVLNCGSSSLKFAVIDATTGDDLLSGLAECFNLDDARIKWKLNGEKGSADLGAGAAHQEALDYIVNKILPLNPEL
SQNLIAIGHRVVHGGERFTSSVVICDDVIAGIEAAVPYAPLHNPAHLIGIREARRIFPALADKNVAVFDTAFHQTMPEEA
FLYALPYKLYKENGIRRYGAHGTSHYYISLEAAKQLNKPVDELNIINCHLGNGGSVCAIKNGKSVDTSMGLTPLEGLVMG
TRSGDLDPAIIFFLHDKLGMSVADINTMLTKESGLQGLTEVTSDCRFVEDNYDSKEEAKRAMDVYCYRLAKYIGAYAAAM
DGRLDAVVFTGGIGENSAPIREITLNKLGLLGFDVDHDANLAARFGKGGEITKAGSTKALVIPTNEEWVIAHDALELVGA

Sequences:

>Translated_400_residues
MSKLVLVLNCGSSSLKFAVIDATTGDDLLSGLAECFNLDDARIKWKLNGEKGSADLGAGAAHQEALDYIVNKILPLNPEL
SQNLIAIGHRVVHGGERFTSSVVICDDVIAGIEAAVPYAPLHNPAHLIGIREARRIFPALADKNVAVFDTAFHQTMPEEA
FLYALPYKLYKENGIRRYGAHGTSHYYISLEAAKQLNKPVDELNIINCHLGNGGSVCAIKNGKSVDTSMGLTPLEGLVMG
TRSGDLDPAIIFFLHDKLGMSVADINTMLTKESGLQGLTEVTSDCRFVEDNYDSKEEAKRAMDVYCYRLAKYIGAYAAAM
DGRLDAVVFTGGIGENSAPIREITLNKLGLLGFDVDHDANLAARFGKGGEITKAGSTKALVIPTNEEWVIAHDALELVGA
>Mature_399_residues
SKLVLVLNCGSSSLKFAVIDATTGDDLLSGLAECFNLDDARIKWKLNGEKGSADLGAGAAHQEALDYIVNKILPLNPELS
QNLIAIGHRVVHGGERFTSSVVICDDVIAGIEAAVPYAPLHNPAHLIGIREARRIFPALADKNVAVFDTAFHQTMPEEAF
LYALPYKLYKENGIRRYGAHGTSHYYISLEAAKQLNKPVDELNIINCHLGNGGSVCAIKNGKSVDTSMGLTPLEGLVMGT
RSGDLDPAIIFFLHDKLGMSVADINTMLTKESGLQGLTEVTSDCRFVEDNYDSKEEAKRAMDVYCYRLAKYIGAYAAAMD
GRLDAVVFTGGIGENSAPIREITLNKLGLLGFDVDHDANLAARFGKGGEITKAGSTKALVIPTNEEWVIAHDALELVGA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=76.0705289672544, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=44.9748743718593, Blast_Score=327, Evalue=7e-91,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43024; Mature: 42892

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAVIDATTGDDLLSGLAECFNLDDARIKWKLNGEKGSADLGAGA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHQEALDYIVNKILPLNPELSQNLIAIGHRVVHGGERFTSSVVICDDVIAGIEAAVPYAP
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCHHHHCCEEEHHHHHHHHHHCCCCCC
LHNPAHLIGIREARRIFPALADKNVAVFDTAFHQTMPEEAFLYALPYKLYKENGIRRYGA
CCCCHHEEEHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHCCCCEEECC
HGTSHYYISLEAAKQLNKPVDELNIINCHLGNGGSVCAIKNGKSVDTSMGLTPLEGLVMG
CCCEEEEEEEHHHHHCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TRSGDLDPAIIFFLHDKLGMSVADINTMLTKESGLQGLTEVTSDCRFVEDNYDSKEEAKR
CCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCEEECCCCCCHHHHHH
AMDVYCYRLAKYIGAYAAAMDGRLDAVVFTGGIGENSAPIREITLNKLGLLGFDVDHDAN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEHHHCCEEEECCCCCCC
LAARFGKGGEITKAGSTKALVIPTNEEWVIAHDALELVGA
HHEECCCCCCEEECCCCEEEEEECCCCEEEEEHHHHHHCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAVIDATTGDDLLSGLAECFNLDDARIKWKLNGEKGSADLGAGA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHQEALDYIVNKILPLNPELSQNLIAIGHRVVHGGERFTSSVVICDDVIAGIEAAVPYAP
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCHHHHCCEEEHHHHHHHHHHCCCCCC
LHNPAHLIGIREARRIFPALADKNVAVFDTAFHQTMPEEAFLYALPYKLYKENGIRRYGA
CCCCHHEEEHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHCCCCEEECC
HGTSHYYISLEAAKQLNKPVDELNIINCHLGNGGSVCAIKNGKSVDTSMGLTPLEGLVMG
CCCEEEEEEEHHHHHCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TRSGDLDPAIIFFLHDKLGMSVADINTMLTKESGLQGLTEVTSDCRFVEDNYDSKEEAKR
CCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCEEECCCCCCHHHHHH
AMDVYCYRLAKYIGAYAAAMDGRLDAVVFTGGIGENSAPIREITLNKLGLLGFDVDHDAN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEHHHCCEEEECCCCCCC
LAARFGKGGEITKAGSTKALVIPTNEEWVIAHDALELVGA
HHEECCCCCCEEECCCCEEEEEECCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA