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The map label for this gene is rffG [C]

Identifier: 145300225

GI number: 145300225

Start: 3610797

End: 3611807

Strand: Direct

Name: rffG [C]

Synonym: ASA_3338

Alternate gene names: 145300225

Gene position: 3610797-3611807 (Clockwise)

Preceding gene: 145300223

Following gene: 145300226

Centisome position: 76.79

GC content: 64.59

Gene sequence:

>1011_bases
ATGAAGTATCTGGTGACAGGCGCCGCCGGCTTTATCGGCTTTCACGTCGCCAGGCGACTGTGCGAGGCGGGCCATCAGGT
GGTGGGACTGGACAACCTCAACGACTACTACGAGGTAAGCCTGAAACAGGCGCGACTGGCCCGGCTGCTCCCCTTCCCCC
ACTTCCACTTCGAACAGCGGGAGCTGGCAGATCGGGATGCCATGGCAAACCTGTTCACCAGCGGCCAGTTCGAGCGGGTG
ATCCACCTGGGCGCCCAGGCGGGGGTACGTCACTCACTGGAAAATCCGTTCGCCTATGCCGACAGCAATCTCACCGGCAT
GCTGACTGTACTGGAAGGGTGCCGCCAGCACGGCATAAACCATCTGATCTACGCCTCCTCCAGCTCTGTCTATGGCCTGA
GCGATCTGATGCCCTTCTGTGCCGAACAGCGGGTAGATCACCCCGTCTCTCTCTATGCCGCCACCAAGAAGAGCGGCGAG
CTGATGGCCCACGCCTACTCGGCGCTCTACGGCCTGCCCACCACAGGGCTGCGCTTTTTTACCGTCTACGGCCCCTGGGG
TCGCCCCGACATGGCCATCGCCAAGTTCACCCGTGCCATCCTGGCCGGCGAGCCCATCGATGTGTACAACCAGGGCCTGC
TCAGCCGCGACTTTACCTACATAGACGACATAGTGGACGGCATACTCGCCGTCGCCGAACTGCCGCCGCGCGCCAATCCG
CACTGGCGCGCCAGCCAGGGCAGCCTGGCCGAAAGCGCCGCCCCCTACCGCCTCCTCAACATAGGCAATGGCCAGCCGGT
GCGGCTGCTCGACTTCATCGAGGCGCTGGAGCAGGCGCTGGACAAGCCCGCCATCAAGCGGATGCTGCCCATGCAGGCAG
GCGACATGCACGCCACCTGGGCCGACAGCGAGCCCCTTCACACCCTCACGGGCACCCACCCCGCCACCCCGCTCAGGCAG
GGAGTCGCCGAGTTCGTGCGCTGGTATCTCGACTATTACCGGCCGGGGTGA

Upstream 100 bases:

>100_bases
CATGTGGCGCCGGTGCCCGCGCTCTGGTACAAAGCGCCCAGCCCTTTTTCAGCCCCCACTCAGGACCACAGGCCCCCATG
ATCAGCACAGGACAACACTC

Downstream 100 bases:

>100_bases
GCCACGGTGACATGAATGTACAATCCTGACTAAATAGCGCGGCGTGGAACATGAGTCATCACAGGCTTCCCCCATCGCAA
GCCGTTTTTGCTACCCCTTG

Product: UDP-glucuronate 5'-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKYLVTGAAGFIGFHVARRLCEAGHQVVGLDNLNDYYEVSLKQARLARLLPFPHFHFEQRELADRDAMANLFTSGQFERV
IHLGAQAGVRHSLENPFAYADSNLTGMLTVLEGCRQHGINHLIYASSSSVYGLSDLMPFCAEQRVDHPVSLYAATKKSGE
LMAHAYSALYGLPTTGLRFFTVYGPWGRPDMAIAKFTRAILAGEPIDVYNQGLLSRDFTYIDDIVDGILAVAELPPRANP
HWRASQGSLAESAAPYRLLNIGNGQPVRLLDFIEALEQALDKPAIKRMLPMQAGDMHATWADSEPLHTLTGTHPATPLRQ
GVAEFVRWYLDYYRPG

Sequences:

>Translated_336_residues
MKYLVTGAAGFIGFHVARRLCEAGHQVVGLDNLNDYYEVSLKQARLARLLPFPHFHFEQRELADRDAMANLFTSGQFERV
IHLGAQAGVRHSLENPFAYADSNLTGMLTVLEGCRQHGINHLIYASSSSVYGLSDLMPFCAEQRVDHPVSLYAATKKSGE
LMAHAYSALYGLPTTGLRFFTVYGPWGRPDMAIAKFTRAILAGEPIDVYNQGLLSRDFTYIDDIVDGILAVAELPPRANP
HWRASQGSLAESAAPYRLLNIGNGQPVRLLDFIEALEQALDKPAIKRMLPMQAGDMHATWADSEPLHTLTGTHPATPLRQ
GVAEFVRWYLDYYRPG
>Mature_336_residues
MKYLVTGAAGFIGFHVARRLCEAGHQVVGLDNLNDYYEVSLKQARLARLLPFPHFHFEQRELADRDAMANLFTSGQFERV
IHLGAQAGVRHSLENPFAYADSNLTGMLTVLEGCRQHGINHLIYASSSSVYGLSDLMPFCAEQRVDHPVSLYAATKKSGE
LMAHAYSALYGLPTTGLRFFTVYGPWGRPDMAIAKFTRAILAGEPIDVYNQGLLSRDFTYIDDIVDGILAVAELPPRANP
HWRASQGSLAESAAPYRLLNIGNGQPVRLLDFIEALEQALDKPAIKRMLPMQAGDMHATWADSEPLHTLTGTHPATPLRQ
GVAEFVRWYLDYYRPG

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=351, Percent_Identity=25.0712250712251, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=26.0744985673352, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=26.0744985673352, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=26.0744985673352, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=24.2603550295858, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=28.6118980169972, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786974, Length=323, Percent_Identity=25.077399380805, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788589, Length=311, Percent_Identity=25.4019292604502, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788366, Length=205, Percent_Identity=28.780487804878, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=23.0113636363636, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=22.8813559322034, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=239, Percent_Identity=26.3598326359833, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=342, Percent_Identity=22.8070175438596, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=236, Percent_Identity=25.4237288135593, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=320, Percent_Identity=25, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19923002, Length=340, Percent_Identity=24.7058823529412, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356223, Length=340, Percent_Identity=24.1176470588235, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37303; Mature: 37303

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLVTGAAGFIGFHVARRLCEAGHQVVGLDNLNDYYEVSLKQARLARLLPFPHFHFEQR
CCEEEECHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ELADRDAMANLFTSGQFERVIHLGAQAGVRHSLENPFAYADSNLTGMLTVLEGCRQHGIN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCC
HLIYASSSSVYGLSDLMPFCAEQRVDHPVSLYAATKKSGELMAHAYSALYGLPTTGLRFF
EEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMAIAKFTRAILAGEPIDVYNQGLLSRDFTYIDDIVDGILAVAELPPRANP
EEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC
HWRASQGSLAESAAPYRLLNIGNGQPVRLLDFIEALEQALDKPAIKRMLPMQAGDMHATW
CCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEC
ADSEPLHTLTGTHPATPLRQGVAEFVRWYLDYYRPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYLVTGAAGFIGFHVARRLCEAGHQVVGLDNLNDYYEVSLKQARLARLLPFPHFHFEQR
CCEEEECHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ELADRDAMANLFTSGQFERVIHLGAQAGVRHSLENPFAYADSNLTGMLTVLEGCRQHGIN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCC
HLIYASSSSVYGLSDLMPFCAEQRVDHPVSLYAATKKSGELMAHAYSALYGLPTTGLRFF
EEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMAIAKFTRAILAGEPIDVYNQGLLSRDFTYIDDIVDGILAVAELPPRANP
EEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC
HWRASQGSLAESAAPYRLLNIGNGQPVRLLDFIEALEQALDKPAIKRMLPMQAGDMHATW
CCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEC
ADSEPLHTLTGTHPATPLRQGVAEFVRWYLDYYRPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]