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The map label for this gene is ybfF [C]

Identifier: 145299742

GI number: 145299742

Start: 3021941

End: 3022723

Strand: Direct

Name: ybfF [C]

Synonym: ASA_2819

Alternate gene names: 145299742

Gene position: 3021941-3022723 (Clockwise)

Preceding gene: 145299739

Following gene: 145299743

Centisome position: 64.26

GC content: 60.79

Gene sequence:

>783_bases
TTGCAACAAAACTCGTTATTGAACTTCAAGGAACAGGGCCAGGGCCCGGCCGTTGTGCTGATCCACGGCCTGTTCGGCAG
CCTGGACAACCTGGGGCTGCTGGCACGCGCCCTGAGCGAACACTATCGCGTCATCAGCGTGGATCTGCGCAACCACGGCG
CCTCGTTTCACAGCGACGAGATGAGCTACCCCCAGCAGGCTGCCGACGTACTCGCCCTGCTCGATGCCCTGGCCCTGGAT
CAGGTATCTCTGGTCGGTCACTCCATGGGCGGCAAGGTCGCCATGCAACTGGCCAAGCAGGCTCCCGCCAGGGTCGCCCG
GCTGGTGGTGGCCGATATAGCCCCTGTCGCCTATCCCCACTCCCGCCATCAGAATGTGTTCGCCGGACTCAACACCACCC
TGAACCAGCCGGTGCAGAGCCGCAGCGAGGCAGAGGCCATTTTGGCCGAGCACATCGAGATCGCCGGCGTACGCCAGTTC
CTGCTCAAATCCTTCGCCAAGGGAGAGTCCGGTTGGGGCTGGCGTTTCAACGTAGCGGCCCTTGAGCGCAATTACGCCAA
CATCATGGGCTGGCCCGACAACCAGCATCGATTCGAAGGGCCGACACTCTTTATCAAGGGCGGCGATTCGGACTATATGC
AGCCCCAATATACCGACACTGTGATGGCACAATTTCCAGCGGCAAAGGCGCGCGTCATCGCCGGAACAGGCCACTGGCTG
CACGCCGAAAAGCCGGTTTTGTTCAACAAATTGGTTGTGGATTTCCTGTCGACAGGCGGTTAA

Upstream 100 bases:

>100_bases
GAAAAAAAACCACAAAAAAATGACTCTCAAGGAATGAAATCCTTTTCTTTCAGTGTGTTAAAAATCAGGCAAAAAAGCCC
ACGACCGACAAGGTGTCATT

Downstream 100 bases:

>100_bases
CAGTTTCAAATCCTTTGTGATATAGTGCGCCCGTCATAAGGAGTGTGCGCAATCCATGCTAAGTGAACATATCGATCTGA
TTGAGAGTCTGGGCCTGAAG

Product: esterase/lipase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQQNSLLNFKEQGQGPAVVLIHGLFGSLDNLGLLARALSEHYRVISVDLRNHGASFHSDEMSYPQQAADVLALLDALALD
QVSLVGHSMGGKVAMQLAKQAPARVARLVVADIAPVAYPHSRHQNVFAGLNTTLNQPVQSRSEAEAILAEHIEIAGVRQF
LLKSFAKGESGWGWRFNVAALERNYANIMGWPDNQHRFEGPTLFIKGGDSDYMQPQYTDTVMAQFPAAKARVIAGTGHWL
HAEKPVLFNKLVVDFLSTGG

Sequences:

>Translated_260_residues
MQQNSLLNFKEQGQGPAVVLIHGLFGSLDNLGLLARALSEHYRVISVDLRNHGASFHSDEMSYPQQAADVLALLDALALD
QVSLVGHSMGGKVAMQLAKQAPARVARLVVADIAPVAYPHSRHQNVFAGLNTTLNQPVQSRSEAEAILAEHIEIAGVRQF
LLKSFAKGESGWGWRFNVAALERNYANIMGWPDNQHRFEGPTLFIKGGDSDYMQPQYTDTVMAQFPAAKARVIAGTGHWL
HAEKPVLFNKLVVDFLSTGG
>Mature_260_residues
MQQNSLLNFKEQGQGPAVVLIHGLFGSLDNLGLLARALSEHYRVISVDLRNHGASFHSDEMSYPQQAADVLALLDALALD
QVSLVGHSMGGKVAMQLAKQAPARVARLVVADIAPVAYPHSRHQNVFAGLNTTLNQPVQSRSEAEAILAEHIEIAGVRQF
LLKSFAKGESGWGWRFNVAALERNYANIMGWPDNQHRFEGPTLFIKGGDSDYMQPQYTDTVMAQFPAAKARVIAGTGHWL
HAEKPVLFNKLVVDFLSTGG

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=248, Percent_Identity=37.9032258064516, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI23200008, Length=248, Percent_Identity=37.9032258064516, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI223941839, Length=243, Percent_Identity=33.7448559670782, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1786902, Length=236, Percent_Identity=48.728813559322, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17508535, Length=259, Percent_Identity=33.2046332046332, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17507063, Length=254, Percent_Identity=28.740157480315, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321468, Length=266, Percent_Identity=31.9548872180451, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24640348, Length=255, Percent_Identity=29.0196078431373, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646055, Length=267, Percent_Identity=26.5917602996255, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012908 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07819 PGAP1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28393; Mature: 28393

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNSLLNFKEQGQGPAVVLIHGLFGSLDNLGLLARALSEHYRVISVDLRNHGASFHSDE
CCCCCCCCHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC
MSYPQQAADVLALLDALALDQVSLVGHSMGGKVAMQLAKQAPARVARLVVADIAPVAYPH
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRHQNVFAGLNTTLNQPVQSRSEAEAILAEHIEIAGVRQFLLKSFAKGESGWGWRFNVAA
CCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
LERNYANIMGWPDNQHRFEGPTLFIKGGDSDYMQPQYTDTVMAQFPAAKARVIAGTGHWL
HHCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEE
HAEKPVLFNKLVVDFLSTGG
ECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQNSLLNFKEQGQGPAVVLIHGLFGSLDNLGLLARALSEHYRVISVDLRNHGASFHSDE
CCCCCCCCHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC
MSYPQQAADVLALLDALALDQVSLVGHSMGGKVAMQLAKQAPARVARLVVADIAPVAYPH
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRHQNVFAGLNTTLNQPVQSRSEAEAILAEHIEIAGVRQFLLKSFAKGESGWGWRFNVAA
CCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
LERNYANIMGWPDNQHRFEGPTLFIKGGDSDYMQPQYTDTVMAQFPAAKARVIAGTGHWL
HHCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEE
HAEKPVLFNKLVVDFLSTGG
ECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]