| Definition | Aeromonas salmonicida subsp. salmonicida A449, complete genome. |
|---|---|
| Accession | NC_009348 |
| Length | 4,702,402 |
Click here to switch to the map view.
The map label for this gene is dkgB [H]
Identifier: 145299643
GI number: 145299643
Start: 2914617
End: 2915420
Strand: Direct
Name: dkgB [H]
Synonym: ASA_2717
Alternate gene names: 145299643
Gene position: 2914617-2915420 (Clockwise)
Preceding gene: 145299642
Following gene: 145299644
Centisome position: 61.98
GC content: 59.83
Gene sequence:
>804_bases ATGATGAAAATGCCTGCCATGGGTCTGGGAACCTTTCGCCTGAAAGGTGAGCCACTGCTTACGACCCTGAACACCGGTCT GACGCTCGGTTATCGCCATATCGATACCGCCCAGATTTACGACAACGAAAGCGAAGTGGGCGAGGTGCTGGCGACGACCT CGGTGCCCCGCTCGGATATCTACCTCACCACCAAGGTGTGGACCAGCGAGTTTGCCGAAGGCAAGCTGATCCCTAGCCTG AAAACCAGCCTTGAGAAACTGCGCACCGACTATCTGGATCTGGCGCTGATCCACTGGCCCTCCCCCAACGACGAGGTGCC GATGGCGGTCTATCTGGAGCAGCTGGTCGAAGCCAAATCCCTGGGCCTGACCCGGGAAATTGGGGTCTCCAACTTCACGG TTGCCCAGCTTAAAGAAGCCATCGACATTCTGGGTCCCGGCGCCATCGCTCACCAGCAGGTAGAGATCCACCCCTTGCTG CAAAACCGCAAGGTGGTGGAATTCTGCCAGGAGCAGGGCATCGCCATCACCGCCTATATGCCGCTGGCTTACGGCAAGGT GCTGAGTGAGCCGCTCTTGATGGAGATAGGCAAGTACCACAAGGTGTCGGCAGCCCAGGTGTCGCTGGCCTGGCTACTGG CCCAGGGCATGACGGTGATCCCGAGCTCCACCAAGCGGGAGCATCAAGCCGCCAATCTGGCTGCCCTCGAGGTATCCCTG AGCAGTGAAGAGATGGCCCAGATCGCCACCTTGGAGCGGGGTGAACGCTGCGCCGACCCGGACTTTGCACCGGCTTGGGA TTAA
Upstream 100 bases:
>100_bases TTGACGATGGCGCTGCCAACTTGCACCGCCGCCACGGCCTTTGCACCAAGCCAACGCCTTTGCTCTGGGCAAGGGCCAAA CACCCCATTTGAGGAAATTG
Downstream 100 bases:
>100_bases ACTCGGCCCTCACGATCTTTCCCTGCTCAAGGGAGAGTAAAGGCCCCCTTGTGAGGCGCGAGGGTCATAGGGACTCACCC ATGACGACAGCGCCAGAAAG
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 267
Protein sequence:
>267_residues MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL SSEEMAQIATLERGERCADPDFAPAWD
Sequences:
>Translated_267_residues MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL SSEEMAQIATLERGERCADPDFAPAWD >Mature_267_residues MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL SSEEMAQIATLERGERCADPDFAPAWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=294, Percent_Identity=31.6326530612245, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=31.9587628865979, Blast_Score=146, Evalue=1e-35, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=31.3131313131313, Blast_Score=145, Evalue=5e-35, Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=32.1167883211679, Blast_Score=144, Evalue=6e-35, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=33.1058020477816, Blast_Score=144, Evalue=8e-35, Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=31.6151202749141, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI24497577, Length=291, Percent_Identity=31.2714776632302, Blast_Score=143, Evalue=1e-34, Organism=Homo sapiens, GI5174391, Length=291, Percent_Identity=31.2714776632302, Blast_Score=143, Evalue=1e-34, Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=2e-34, Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=2e-34, Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=32.0689655172414, Blast_Score=142, Evalue=4e-34, Organism=Homo sapiens, GI291291012, Length=266, Percent_Identity=32.3308270676692, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=28.1967213114754, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI300116273, Length=270, Percent_Identity=32.962962962963, Blast_Score=129, Evalue=2e-30, Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=32.312925170068, Blast_Score=128, Evalue=5e-30, Organism=Homo sapiens, GI300116271, Length=272, Percent_Identity=31.9852941176471, Blast_Score=122, Evalue=3e-28, Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=31.1688311688312, Blast_Score=78, Evalue=7e-15, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=36.5384615384615, Blast_Score=77, Evalue=1e-14, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=52.059925093633, Blast_Score=293, Evalue=1e-80, Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=33.7164750957854, Blast_Score=161, Evalue=3e-41, Organism=Escherichia coli, GI1788081, Length=266, Percent_Identity=34.9624060150376, Blast_Score=120, Evalue=1e-28, Organism=Escherichia coli, GI1787674, Length=253, Percent_Identity=25.6916996047431, Blast_Score=69, Evalue=2e-13, Organism=Escherichia coli, GI87081735, Length=292, Percent_Identity=27.3972602739726, Blast_Score=67, Evalue=8e-13, Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=24.9211356466877, Blast_Score=66, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=2e-37, Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=1e-36, Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=31.9852941176471, Blast_Score=144, Evalue=3e-35, Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=33.5766423357664, Blast_Score=143, Evalue=7e-35, Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=32.8301886792453, Blast_Score=140, Evalue=1e-33, Organism=Caenorhabditis elegans, GI17561300, Length=261, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=30.2405498281787, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=30.8510638297872, Blast_Score=125, Evalue=2e-29, Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=32.1033210332103, Blast_Score=123, Evalue=8e-29, Organism=Caenorhabditis elegans, GI17564128, Length=301, Percent_Identity=30.8970099667774, Blast_Score=123, Evalue=9e-29, Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=27.9461279461279, Blast_Score=117, Evalue=5e-27, Organism=Caenorhabditis elegans, GI17562292, Length=283, Percent_Identity=27.9151943462898, Blast_Score=107, Evalue=7e-24, Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=34.0136054421769, Blast_Score=163, Evalue=3e-41, Organism=Saccharomyces cerevisiae, GI6320576, Length=278, Percent_Identity=32.0143884892086, Blast_Score=147, Evalue=2e-36, Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=28.6274509803922, Blast_Score=123, Evalue=3e-29, Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=29.8701298701299, Blast_Score=116, Evalue=4e-27, Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=32.3741007194245, Blast_Score=101, Evalue=1e-22, Organism=Saccharomyces cerevisiae, GI6319625, Length=260, Percent_Identity=31.9230769230769, Blast_Score=100, Evalue=2e-22, Organism=Drosophila melanogaster, GI24644950, Length=305, Percent_Identity=33.7704918032787, Blast_Score=150, Evalue=1e-36, Organism=Drosophila melanogaster, GI221378297, Length=325, Percent_Identity=32, Blast_Score=143, Evalue=1e-34, Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=33.1034482758621, Blast_Score=137, Evalue=5e-33, Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=32.8719723183391, Blast_Score=134, Evalue=5e-32, Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=33.1034482758621, Blast_Score=132, Evalue=3e-31, Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=29.3515358361775, Blast_Score=130, Evalue=8e-31, Organism=Drosophila melanogaster, GI24663317, Length=287, Percent_Identity=29.9651567944251, Blast_Score=125, Evalue=3e-29, Organism=Drosophila melanogaster, GI20129731, Length=279, Percent_Identity=30.4659498207885, Blast_Score=120, Evalue=7e-28, Organism=Drosophila melanogaster, GI281366140, Length=264, Percent_Identity=32.9545454545455, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=31.1418685121107, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=30.7958477508651, Blast_Score=106, Evalue=2e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29365; Mature: 29365
Theoretical pI: Translated: 4.79; Mature: 4.79
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 3.4 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 4.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDI CCCCCCCCCCEEEECCCCEEEEECCCEEECEEECCHHEEECCHHHHHHHHHHCCCCCCCE YLTTKVWTSEFAEGKLIPSLKTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKS EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH LGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLLQNRKVVEFCQEQGIAITAYM CCCCHHCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEEHHHHCCHHHHHHHHCCCEEEEEH PLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHCCEEEEEEEC SSEEMAQIATLERGERCADPDFAPAWD CHHHHHHHHHHHCCCCCCCCCCCCCCC >Mature Secondary Structure MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDI CCCCCCCCCCEEEECCCCEEEEECCCEEECEEECCHHEEECCHHHHHHHHHHCCCCCCCE YLTTKVWTSEFAEGKLIPSLKTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKS EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH LGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLLQNRKVVEFCQEQGIAITAYM CCCCHHCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEEHHHHCCHHHHHHHHCCCEEEEEH PLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHCCEEEEEEEC SSEEMAQIATLERGERCADPDFAPAWD CHHHHHHHHHHHCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]