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The map label for this gene is asnB [H]

Identifier: 145299448

GI number: 145299448

Start: 2678236

End: 2679900

Strand: Direct

Name: asnB [H]

Synonym: ASA_2510

Alternate gene names: 145299448

Gene position: 2678236-2679900 (Clockwise)

Preceding gene: 145299447

Following gene: 145299450

Centisome position: 56.95

GC content: 60.24

Gene sequence:

>1665_bases
ATGTGTTCTATTTTCGGCATTCTGGACATCAAATCCGATGCAGCAGCCTTGCGCAAATCGGCGCTGGAGATGTCCAAGCG
ATTGCGTCACCGCGGTCCTGACTGGTCCGGCATCTACAGCTCAGACAAAGCCATACTGGTGCACGAGCGCCTGGCCATCG
TCGATCCGGGCAATGGCGCCCAGCCGCTCTACAACCCGGAGCGCACTCACGTATTGGCCGTCAACGGCGAGATCTACAAC
CACAAGGAACTCGAAAAGAACCTCAAGGTCGACTTCCAGTTCCAGACCCACTCCGACTGCGAGGTGCTGCTGGCACTCTA
CAAGGAGAAGGGCCCCGCCTTTTTGGACGATCTCAACGGCATCTTCGCCTTCATCCTCTATGACGCCGAGCAAGATGCCT
ACCTGATTGGTCGTGACCACATGGGGATCATCCCCCTCTATACCGGTCGCGACGAGCACGGCAACTTCTATGTCGCCTCC
GAGATGAAAGCCCTGGTGCCGGTTTGCAAGAGCGTCGAAACCTTCCCGCCCGGACATTACCTCTGGAGCAAGGATGGCGA
GCTCAAGCAGTGGTACAAGCGCGACTGGATGGAATACGGCGCGGTCGAGCACAAGGTCAGCGACAGAGCGGAACTGAAAG
CCGCGCTGGAAGCGGCCGTCAAACGCCAGCTGATGTGCGACGTGCCCTATGGCGTGCTGCTCTCCGGCGGGCTGGATTCG
TCCGTCATCTCCGCCATCACCAAGATCTATGCCGCCCGCCGGGTGGAAGACGATGGCAAGAGCGAGGCATGGTGGCCGCA
GCTGCACTCCTTTGCCGTCGGGCTGGAAGGCTCGCCGGATCTCGCCGCCGCCCGCAAGGTGGCTGACCATCTGGGTACCA
TACACCACCAGATCCACTTCACCGTGCAGGAGGGGCTGGATGCCCTGCGGGACGTCATCTATCACCTCGAGACCTATGAC
GTCACCACCATCCGCGCCTCTACCCCCATGTACCTGATGGCCCGTTACATCAAGGCGATGGGCATCAAGATGGTGCTCTC
CGGTGAAGGTTCCGACGAGCTGTTCGGTGGTTATCTCTACTTCCACAAGGCGCCCAACGCCAAAGAGTTCCACGAAGAGA
ACGTGCGCAAGCTTGCCTCCCTGCATCTCTATGACTGCCTGCGGGCCAACAAGTCCATGGCCGCCTGGGGTGTGGAAGGC
CGCGTGCCCTTCCTCGACAAGGAGTTCATGGACGTGGCGATGCGCCTGCAGCCTGCTGACAAGATGTGTGGCAACGGCAA
GATCGAGAAGCACATACTGCGTGAAGCTTTCGAGGAGCTGTTGCCCCACGAAGTGGCCTGGCGCCAGAAGGAGCAGTTCT
CCGACGGCGTGGGTTACTCCTGGATTGACAGCCTCAAGGCCATGGTCGAGGTCGAAGTGACGGATCAGATGTTCGAGGCG
GCGGCATTCCGCTTCCCCGTCAATACACCGCTCACCAAGGAAGCCTATTTTTATCGCGCCATCTTCGACGAGCACTTCCC
GCTGGAGTCCGCCGCCCGTACCGTGCCCTACGGCAAGTCGGTCGCCTGCTCCACCCCGACCGCCTTGGAGTGGGATGCCA
AATTCAAGGAGATGGCAGACCCGTCCGGTCGCGCCGTGATGGACGTCCACCAGCAGAGTTACTGA

Upstream 100 bases:

>100_bases
AAAGGTTTTTGCGCTAGGCATTGTGATTGACGAGGAATGTAAATGGAATTTCGAGCCCGTATGTGGGGGGCTCCTATAAA
AATCAAGAGGATTACCGCCA

Downstream 100 bases:

>100_bases
TCCGCTGACGGATATCCTGGCCAACCCGCCAACAACAAAGCCCCTCGCGAGGGGCTTTATTTTTTGGCCATGGCGATGGC
GCTCGTCAGCTCAGCTGGCC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFGILDIKSDAAALRKSALEMSKRLRHRGPDWSGIYSSDKAILVHERLAIVDPGNGAQPLYNPERTHVLAVNGEIYN
HKELEKNLKVDFQFQTHSDCEVLLALYKEKGPAFLDDLNGIFAFILYDAEQDAYLIGRDHMGIIPLYTGRDEHGNFYVAS
EMKALVPVCKSVETFPPGHYLWSKDGELKQWYKRDWMEYGAVEHKVSDRAELKAALEAAVKRQLMCDVPYGVLLSGGLDS
SVISAITKIYAARRVEDDGKSEAWWPQLHSFAVGLEGSPDLAAARKVADHLGTIHHQIHFTVQEGLDALRDVIYHLETYD
VTTIRASTPMYLMARYIKAMGIKMVLSGEGSDELFGGYLYFHKAPNAKEFHEENVRKLASLHLYDCLRANKSMAAWGVEG
RVPFLDKEFMDVAMRLQPADKMCGNGKIEKHILREAFEELLPHEVAWRQKEQFSDGVGYSWIDSLKAMVEVEVTDQMFEA
AAFRFPVNTPLTKEAYFYRAIFDEHFPLESAARTVPYGKSVACSTPTALEWDAKFKEMADPSGRAVMDVHQQSY

Sequences:

>Translated_554_residues
MCSIFGILDIKSDAAALRKSALEMSKRLRHRGPDWSGIYSSDKAILVHERLAIVDPGNGAQPLYNPERTHVLAVNGEIYN
HKELEKNLKVDFQFQTHSDCEVLLALYKEKGPAFLDDLNGIFAFILYDAEQDAYLIGRDHMGIIPLYTGRDEHGNFYVAS
EMKALVPVCKSVETFPPGHYLWSKDGELKQWYKRDWMEYGAVEHKVSDRAELKAALEAAVKRQLMCDVPYGVLLSGGLDS
SVISAITKIYAARRVEDDGKSEAWWPQLHSFAVGLEGSPDLAAARKVADHLGTIHHQIHFTVQEGLDALRDVIYHLETYD
VTTIRASTPMYLMARYIKAMGIKMVLSGEGSDELFGGYLYFHKAPNAKEFHEENVRKLASLHLYDCLRANKSMAAWGVEG
RVPFLDKEFMDVAMRLQPADKMCGNGKIEKHILREAFEELLPHEVAWRQKEQFSDGVGYSWIDSLKAMVEVEVTDQMFEA
AAFRFPVNTPLTKEAYFYRAIFDEHFPLESAARTVPYGKSVACSTPTALEWDAKFKEMADPSGRAVMDVHQQSY
>Mature_554_residues
MCSIFGILDIKSDAAALRKSALEMSKRLRHRGPDWSGIYSSDKAILVHERLAIVDPGNGAQPLYNPERTHVLAVNGEIYN
HKELEKNLKVDFQFQTHSDCEVLLALYKEKGPAFLDDLNGIFAFILYDAEQDAYLIGRDHMGIIPLYTGRDEHGNFYVAS
EMKALVPVCKSVETFPPGHYLWSKDGELKQWYKRDWMEYGAVEHKVSDRAELKAALEAAVKRQLMCDVPYGVLLSGGLDS
SVISAITKIYAARRVEDDGKSEAWWPQLHSFAVGLEGSPDLAAARKVADHLGTIHHQIHFTVQEGLDALRDVIYHLETYD
VTTIRASTPMYLMARYIKAMGIKMVLSGEGSDELFGGYLYFHKAPNAKEFHEENVRKLASLHLYDCLRANKSMAAWGVEG
RVPFLDKEFMDVAMRLQPADKMCGNGKIEKHILREAFEELLPHEVAWRQKEQFSDGVGYSWIDSLKAMVEVEVTDQMFEA
AAFRFPVNTPLTKEAYFYRAIFDEHFPLESAARTVPYGKSVACSTPTALEWDAKFKEMADPSGRAVMDVHQQSY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=542, Percent_Identity=40.4059040590406, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI168229252, Length=542, Percent_Identity=40.4059040590406, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI168229250, Length=542, Percent_Identity=40.4059040590406, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI296010848, Length=516, Percent_Identity=41.8604651162791, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI296010852, Length=448, Percent_Identity=43.0803571428571, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI296010850, Length=448, Percent_Identity=43.0803571428571, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=73.6462093862816, Blast_Score=872, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=569, Percent_Identity=43.7609841827768, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=43.649373881932, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=35.5640535372849, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI25147560, Length=311, Percent_Identity=38.5852090032154, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321563, Length=579, Percent_Identity=48.7046632124352, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325403, Length=580, Percent_Identity=48.2758620689655, Blast_Score=523, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=47.313691507799, Blast_Score=489, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62638; Mature: 62638

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGILDIKSDAAALRKSALEMSKRLRHRGPDWSGIYSSDKAILVHERLAIVDPGNGA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCC
QPLYNPERTHVLAVNGEIYNHKELEKNLKVDFQFQTHSDCEVLLALYKEKGPAFLDDLNG
CCCCCCCCCEEEEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCC
IFAFILYDAEQDAYLIGRDHMGIIPLYTGRDEHGNFYVASEMKALVPVCKSVETFPPGHY
EEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCE
LWSKDGELKQWYKRDWMEYGAVEHKVSDRAELKAALEAAVKRQLMCDVPYGVLLSGGLDS
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCH
SVISAITKIYAARRVEDDGKSEAWWPQLHSFAVGLEGSPDLAAARKVADHLGTIHHQIHF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TVQEGLDALRDVIYHLETYDVTTIRASTPMYLMARYIKAMGIKMVLSGEGSDELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEEE
FHKAPNAKEFHEENVRKLASLHLYDCLRANKSMAAWGVEGRVPFLDKEFMDVAMRLQPAD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCHH
KMCGNGKIEKHILREAFEELLPHEVAWRQKEQFSDGVGYSWIDSLKAMVEVEVTDQMFEA
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHEEEHHHHHHHHH
AAFRFPVNTPLTKEAYFYRAIFDEHFPLESAARTVPYGKSVACSTPTALEWDAKFKEMAD
HHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCCCEECCCHHHHHCC
PSGRAVMDVHQQSY
CCCCEEEHHHHCCC
>Mature Secondary Structure
MCSIFGILDIKSDAAALRKSALEMSKRLRHRGPDWSGIYSSDKAILVHERLAIVDPGNGA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCC
QPLYNPERTHVLAVNGEIYNHKELEKNLKVDFQFQTHSDCEVLLALYKEKGPAFLDDLNG
CCCCCCCCCEEEEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCC
IFAFILYDAEQDAYLIGRDHMGIIPLYTGRDEHGNFYVASEMKALVPVCKSVETFPPGHY
EEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCE
LWSKDGELKQWYKRDWMEYGAVEHKVSDRAELKAALEAAVKRQLMCDVPYGVLLSGGLDS
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCH
SVISAITKIYAARRVEDDGKSEAWWPQLHSFAVGLEGSPDLAAARKVADHLGTIHHQIHF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TVQEGLDALRDVIYHLETYDVTTIRASTPMYLMARYIKAMGIKMVLSGEGSDELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEEE
FHKAPNAKEFHEENVRKLASLHLYDCLRANKSMAAWGVEGRVPFLDKEFMDVAMRLQPAD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCHH
KMCGNGKIEKHILREAFEELLPHEVAWRQKEQFSDGVGYSWIDSLKAMVEVEVTDQMFEA
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHEEEHHHHHHHHH
AAFRFPVNTPLTKEAYFYRAIFDEHFPLESAARTVPYGKSVACSTPTALEWDAKFKEMAD
HHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCCCEECCCHHHHHCC
PSGRAVMDVHQQSY
CCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]