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The map label for this gene is sapA [H]

Identifier: 145299378

GI number: 145299378

Start: 2587778

End: 2589373

Strand: Direct

Name: sapA [H]

Synonym: ASA_2427

Alternate gene names: 145299378

Gene position: 2587778-2589373 (Clockwise)

Preceding gene: 145299377

Following gene: 145299379

Centisome position: 55.03

GC content: 60.28

Gene sequence:

>1596_bases
ATGCTCAAACCCTTGCTGCTTGGATTGCTGGTGCTGGTTACCGGTTGTCAGAAACAAGACGACGCGTCCAGGACGGGACT
CATCTACTGTGCGGAAGGCTCGCCCCAGACCTTCAATCCCCAGGTCTCCAACTCCGGGGTGACCCTGGATGCCAGCTCCC
GCCCGCTCTACGATCGTCTGCTGGAGGTGAACCCCAACACGCTGGCGCTGGAAGGGGGGCTCGCCACCCGCTGGAACGTG
AGTGAAGATGGTCTCTCCTACACCCTGACTCTGCGCAAGGGCGTCACCTTTCACCACACCCCCTGGTTCAATCCCTCACG
CAACCTGAATGCCGACGATGTGCTGTTCACCTGGCAGCGGCTGCTGGACAAGCAGCACCCCTATCACGCCATTTCGGGGG
GCGAGTATCCCTACTTCTACAGCCTGGGGCTGGATCAACTGATCAAGCGGATCTACAAGAAGGGACAGCAGCAGGTGGTG
TTTGAACTGAATCGGCCGGATGCCTCCTTCCTCGCCACGCTGGCCAGCGACTACGCCATAGTGCTCTCCGCCGAATATGC
CGACCAGATGCGCAGCGCCGGCACACAGGCACTGCTGGACACTCAACCGGTCGGGACAGGTCCGTTCAGCTTCAAGGAGT
ATCGCCACAACGAATATGTGCGTTACCTGCGCCACCCGGGTTACTGGGGCGGCGAAGCCAAGATTGAACAACTGGTCTAT
GACATCACGCCAAAATCCGCCAAGCGACTGGCCAAGCTACTGACGGGCGAGTGCGATGTGATGAGTACCCCCGTTGCCAG
CCAGTTGTCGGTGATCAAGCAGAACCCGAAGCTGAGCCTGTCCATACAGTCGGGCATGAACGTGGCCTTCCTTGCCCTCA
ACACCCGCAAGGCACCCTTCAACAAGGTACAGGTACGTCAGGCCATCGCCAGTGCCATCAATATCGACAACCTGTTGCAA
GCGGTCTACTTCGAGACAGGACAACCGGCCAACAGCCTGCTCCCTCCCCTCTCCTGGGGCTACAATCCTTCTCTGCCTAT
GCGTCAGCAAGACTTGAAGCGATCACGTCAGCTGCTCAAGGAGGCCGGGTTTGAGCACGGATTCGAGATGCAGGTGCTGG
TACAGCCAGGCTCGCGCCCCTACAACCCGGATGCCCTCAAGACAGCCCAGCTGATGCGCAGCGATCTGGCCAAGGTCGGC
ATCAAGCTGAAAATCGTGCAGCAGGAATGGCCTGTCATCGAGACGCGCCTCGCCCATGGCCACTATGACAGCCTGCTGTC
GGGCTGGATTGCCGACAACGCCGACCCCGACAACTTCTTTCGCCAGTTGCTGAGCTGCACCGCCGTCGAGCGTGGCAACA
ACTACAGCCGCTGGTGCAGCCCGGCCTTCGATCAACTGCTGGATGACGCTGTGACCACCCCCCAGCTGGCGTTCCGGCTG
CGCAACTACTATTACGCCCAGACCCTGCTCAACGAGCAGTTGCCGCTCATCCCGCTGGCCCATGCCCTGCGCACCCAGGT
CAGTCGCAGCGACATTGAGGGGCTCTCCCTGATGCCTTTTGGCGGCACCGTCTTCTACCAAGCACACAGGGAGTAA

Upstream 100 bases:

>100_bases
TGCGGATTGAAACCACAGTAAGCCCGGTTCAGGCCGACTCCTGCAACTCGCCAGCCCCGATGCTGGCACATTTGTATAAA
AACATGGTTCTACAATGAGA

Downstream 100 bases:

>100_bases
GCATGTTTCTCTATATTCTGCGCCGCATCAGCCTGCTGCTCATTACCCTGCTGGCCCTGACCTTTGTCGCCTACCTGCTG
GATTATCGGCTCATCGGCCT

Product: ABC-type peptide transporter, periplasmic binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MLKPLLLGLLVLVTGCQKQDDASRTGLIYCAEGSPQTFNPQVSNSGVTLDASSRPLYDRLLEVNPNTLALEGGLATRWNV
SEDGLSYTLTLRKGVTFHHTPWFNPSRNLNADDVLFTWQRLLDKQHPYHAISGGEYPYFYSLGLDQLIKRIYKKGQQQVV
FELNRPDASFLATLASDYAIVLSAEYADQMRSAGTQALLDTQPVGTGPFSFKEYRHNEYVRYLRHPGYWGGEAKIEQLVY
DITPKSAKRLAKLLTGECDVMSTPVASQLSVIKQNPKLSLSIQSGMNVAFLALNTRKAPFNKVQVRQAIASAINIDNLLQ
AVYFETGQPANSLLPPLSWGYNPSLPMRQQDLKRSRQLLKEAGFEHGFEMQVLVQPGSRPYNPDALKTAQLMRSDLAKVG
IKLKIVQQEWPVIETRLAHGHYDSLLSGWIADNADPDNFFRQLLSCTAVERGNNYSRWCSPAFDQLLDDAVTTPQLAFRL
RNYYYAQTLLNEQLPLIPLAHALRTQVSRSDIEGLSLMPFGGTVFYQAHRE

Sequences:

>Translated_531_residues
MLKPLLLGLLVLVTGCQKQDDASRTGLIYCAEGSPQTFNPQVSNSGVTLDASSRPLYDRLLEVNPNTLALEGGLATRWNV
SEDGLSYTLTLRKGVTFHHTPWFNPSRNLNADDVLFTWQRLLDKQHPYHAISGGEYPYFYSLGLDQLIKRIYKKGQQQVV
FELNRPDASFLATLASDYAIVLSAEYADQMRSAGTQALLDTQPVGTGPFSFKEYRHNEYVRYLRHPGYWGGEAKIEQLVY
DITPKSAKRLAKLLTGECDVMSTPVASQLSVIKQNPKLSLSIQSGMNVAFLALNTRKAPFNKVQVRQAIASAINIDNLLQ
AVYFETGQPANSLLPPLSWGYNPSLPMRQQDLKRSRQLLKEAGFEHGFEMQVLVQPGSRPYNPDALKTAQLMRSDLAKVG
IKLKIVQQEWPVIETRLAHGHYDSLLSGWIADNADPDNFFRQLLSCTAVERGNNYSRWCSPAFDQLLDDAVTTPQLAFRL
RNYYYAQTLLNEQLPLIPLAHALRTQVSRSDIEGLSLMPFGGTVFYQAHRE
>Mature_531_residues
MLKPLLLGLLVLVTGCQKQDDASRTGLIYCAEGSPQTFNPQVSNSGVTLDASSRPLYDRLLEVNPNTLALEGGLATRWNV
SEDGLSYTLTLRKGVTFHHTPWFNPSRNLNADDVLFTWQRLLDKQHPYHAISGGEYPYFYSLGLDQLIKRIYKKGQQQVV
FELNRPDASFLATLASDYAIVLSAEYADQMRSAGTQALLDTQPVGTGPFSFKEYRHNEYVRYLRHPGYWGGEAKIEQLVY
DITPKSAKRLAKLLTGECDVMSTPVASQLSVIKQNPKLSLSIQSGMNVAFLALNTRKAPFNKVQVRQAIASAINIDNLLQ
AVYFETGQPANSLLPPLSWGYNPSLPMRQQDLKRSRQLLKEAGFEHGFEMQVLVQPGSRPYNPDALKTAQLMRSDLAKVG
IKLKIVQQEWPVIETRLAHGHYDSLLSGWIADNADPDNFFRQLLSCTAVERGNNYSRWCSPAFDQLLDDAVTTPQLAFRL
RNYYYAQTLLNEQLPLIPLAHALRTQVSRSDIEGLSLMPFGGTVFYQAHRE

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=525, Percent_Identity=43.2380952380952, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787551, Length=511, Percent_Identity=44.0313111545988, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787762, Length=532, Percent_Identity=23.6842105263158, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787052, Length=502, Percent_Identity=24.1035856573705, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789887, Length=470, Percent_Identity=22.3404255319149, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787495, Length=517, Percent_Identity=22.4371373307544, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081878, Length=449, Percent_Identity=24.0534521158129, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789397, Length=469, Percent_Identity=23.0277185501066, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59635; Mature: 59635

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPLLLGLLVLVTGCQKQDDASRTGLIYCAEGSPQTFNPQVSNSGVTLDASSRPLYDRL
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHH
LEVNPNTLALEGGLATRWNVSEDGLSYTLTLRKGVTFHHTPWFNPSRNLNADDVLFTWQR
HHCCCCEEEEECCCCEECCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHH
LLDKQHPYHAISGGEYPYFYSLGLDQLIKRIYKKGQQQVVFELNRPDASFLATLASDYAI
HHCCCCCCEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCEEE
VLSAEYADQMRSAGTQALLDTQPVGTGPFSFKEYRHNEYVRYLRHPGYWGGEAKIEQLVY
EEEHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
DITPKSAKRLAKLLTGECDVMSTPVASQLSVIKQNPKLSLSIQSGMNVAFLALNTRKAPF
HCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCC
NKVQVRQAIASAINIDNLLQAVYFETGQPANSLLPPLSWGYNPSLPMRQQDLKRSRQLLK
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EAGFEHGFEMQVLVQPGSRPYNPDALKTAQLMRSDLAKVGIKLKIVQQEWPVIETRLAHG
HCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHCC
HYDSLLSGWIADNADPDNFFRQLLSCTAVERGNNYSRWCSPAFDQLLDDAVTTPQLAFRL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCHHHHHHH
RNYYYAQTLLNEQLPLIPLAHALRTQVSRSDIEGLSLMPFGGTVFYQAHRE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCC
>Mature Secondary Structure
MLKPLLLGLLVLVTGCQKQDDASRTGLIYCAEGSPQTFNPQVSNSGVTLDASSRPLYDRL
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHH
LEVNPNTLALEGGLATRWNVSEDGLSYTLTLRKGVTFHHTPWFNPSRNLNADDVLFTWQR
HHCCCCEEEEECCCCEECCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHH
LLDKQHPYHAISGGEYPYFYSLGLDQLIKRIYKKGQQQVVFELNRPDASFLATLASDYAI
HHCCCCCCEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCEEE
VLSAEYADQMRSAGTQALLDTQPVGTGPFSFKEYRHNEYVRYLRHPGYWGGEAKIEQLVY
EEEHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
DITPKSAKRLAKLLTGECDVMSTPVASQLSVIKQNPKLSLSIQSGMNVAFLALNTRKAPF
HCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCC
NKVQVRQAIASAINIDNLLQAVYFETGQPANSLLPPLSWGYNPSLPMRQQDLKRSRQLLK
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EAGFEHGFEMQVLVQPGSRPYNPDALKTAQLMRSDLAKVGIKLKIVQQEWPVIETRLAHG
HCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHCC
HYDSLLSGWIADNADPDNFFRQLLSCTAVERGNNYSRWCSPAFDQLLDDAVTTPQLAFRL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCHHHHHHH
RNYYYAQTLLNEQLPLIPLAHALRTQVSRSDIEGLSLMPFGGTVFYQAHRE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]