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The map label for this gene is pspF [H]

Identifier: 145299377

GI number: 145299377

Start: 2586561

End: 2587595

Strand: Direct

Name: pspF [H]

Synonym: ASA_2426

Alternate gene names: 145299377

Gene position: 2586561-2587595 (Clockwise)

Preceding gene: 145299365

Following gene: 145299378

Centisome position: 55.01

GC content: 61.55

Gene sequence:

>1035_bases
ATGAGCACGGCCACGGAAAGCCTGTTGGGGGAATCCAACGCATTCCTCGAGATCATTGAGCAGGCCTCCCGCCTCGCACC
GCTGCGCAAACCCGTGCTGATCATCGGCGAACGGGGAACGGGCAAGGAGCTCATCGCCCATCGCCTACATTATCTCTCGG
GGCGTTGGGATCAAAGCTTTGTCACCATCAACTGCGCCACCTTGAGTGAGAGCCTGCTGGAAACCGAGCTGTTCGGCCAC
GAGGCAGGCTCCTTCACAGGCGCCGCCAAGCGCCATCAGGGCCGGTTCGAGCGCGCCGATGGCGGCACTCTGTTCCTCGA
CGAGCTGGCAACCACCAGCGCCCGGGTACAGGAAAAACTGCTGCGGGTCATCGAATACGGTGAATTCGAGCGAGTGGGTG
GGGCCAAGGCCCTCAAGGTGGATGTACGGCTGGTGTGTGCCACCAACGAGGATCTGCCGGCGTTGGCCGACAAGGGGCAC
TTTCGCCACGACTTGCTGGACAGGCTTGCCTTTGATGTCATCACTCTGCCCCCCTTGCGGGAGCGGCGCGAGGACATACT
GATGCTGGCGGAGCACTTCGCCCACGGCATGACCCGGGAACTGGGTTATCCCCTGTTTGCCGGATTTTCGCGCAAGGCGA
CCCAGCTGCTGCTGGATTACCCCTGGCCCGGCAACGTGCGGGAGCTCAAGAACGTGGTGGAGCGCAGCATCTATCGTCAG
GGTAATCCCCAGTTGCCGCTCTCGGATCTGGTGCTCAACCCGTTCGATTCCCCCTGGCGCCCCCGTGCGCAAGGCACCCC
TGCCACGCACATCGCCAGTGAGGGCCTGCCACAGGCTAACCCGGCCGCTCAGAGCCTGCCGCTGGATCTCAAAAACGCGG
TGGCGGAGTACGAGATTAATTTACTCAAGCAAGCCATGCAGCAATCCCAGTATAATCAGCGCAAAGCCGCCCAGTTGCTG
GCCCTGAGTTATCACCAGTTCCGTGGCATGCTGCGCAAATACCAGCTGCTGGACAGTGATAACGATGCGGATTGA

Upstream 100 bases:

>100_bases
GGGCCTATGGGTCGAGTTTCAAGATACCCCATGCAAGTGATGATTAAAATCACCAACTTTTGGTAAGATTGACCAGCTTT
TACAACTGACAGGGATCACT

Downstream 100 bases:

>100_bases
CGCCACAGGAAATGCTGGCCCTGCGCTATCACCAGTGCCGTGGCATGCGGCGCAAATACCCGCTGCAGGATAGTGATAAC
GATGCGGATTGAAACCACAG

Product: psp operon transcriptional activator PspF

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTATESLLGESNAFLEIIEQASRLAPLRKPVLIIGERGTGKELIAHRLHYLSGRWDQSFVTINCATLSESLLETELFGH
EAGSFTGAAKRHQGRFERADGGTLFLDELATTSARVQEKLLRVIEYGEFERVGGAKALKVDVRLVCATNEDLPALADKGH
FRHDLLDRLAFDVITLPPLRERREDILMLAEHFAHGMTRELGYPLFAGFSRKATQLLLDYPWPGNVRELKNVVERSIYRQ
GNPQLPLSDLVLNPFDSPWRPRAQGTPATHIASEGLPQANPAAQSLPLDLKNAVAEYEINLLKQAMQQSQYNQRKAAQLL
ALSYHQFRGMLRKYQLLDSDNDAD

Sequences:

>Translated_344_residues
MSTATESLLGESNAFLEIIEQASRLAPLRKPVLIIGERGTGKELIAHRLHYLSGRWDQSFVTINCATLSESLLETELFGH
EAGSFTGAAKRHQGRFERADGGTLFLDELATTSARVQEKLLRVIEYGEFERVGGAKALKVDVRLVCATNEDLPALADKGH
FRHDLLDRLAFDVITLPPLRERREDILMLAEHFAHGMTRELGYPLFAGFSRKATQLLLDYPWPGNVRELKNVVERSIYRQ
GNPQLPLSDLVLNPFDSPWRPRAQGTPATHIASEGLPQANPAAQSLPLDLKNAVAEYEINLLKQAMQQSQYNQRKAAQLL
ALSYHQFRGMLRKYQLLDSDNDAD
>Mature_343_residues
STATESLLGESNAFLEIIEQASRLAPLRKPVLIIGERGTGKELIAHRLHYLSGRWDQSFVTINCATLSESLLETELFGHE
AGSFTGAAKRHQGRFERADGGTLFLDELATTSARVQEKLLRVIEYGEFERVGGAKALKVDVRLVCATNEDLPALADKGHF
RHDLLDRLAFDVITLPPLRERREDILMLAEHFAHGMTRELGYPLFAGFSRKATQLLLDYPWPGNVRELKNVVERSIYRQG
NPQLPLSDLVLNPFDSPWRPRAQGTPATHIASEGLPQANPAAQSLPLDLKNAVAEYEINLLKQAMQQSQYNQRKAAQLLA
LSYHQFRGMLRKYQLLDSDNDAD

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=336, Percent_Identity=61.0119047619048, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1788550, Length=333, Percent_Identity=39.039039039039, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1790437, Length=230, Percent_Identity=49.5652173913044, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=47.8260869565217, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789087, Length=270, Percent_Identity=45.1851851851852, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1790299, Length=325, Percent_Identity=40.9230769230769, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=37.202380952381, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI87082152, Length=339, Percent_Identity=35.1032448377581, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788905, Length=335, Percent_Identity=35.2238805970149, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787583, Length=340, Percent_Identity=34.4117647058824, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=43.9834024896266, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=36.0515021459227, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081858, Length=220, Percent_Identity=31.3636363636364, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 38567; Mature: 38436

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATESLLGESNAFLEIIEQASRLAPLRKPVLIIGERGTGKELIAHRLHYLSGRWDQSF
CCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCE
VTINCATLSESLLETELFGHEAGSFTGAAKRHQGRFERADGGTLFLDELATTSARVQEKL
EEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
LRVIEYGEFERVGGAKALKVDVRLVCATNEDLPALADKGHFRHDLLDRLAFDVITLPPLR
HHHHHCCCHHHCCCCEEEEEEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHH
ERREDILMLAEHFAHGMTRELGYPLFAGFSRKATQLLLDYPWPGNVRELKNVVERSIYRQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHC
GNPQLPLSDLVLNPFDSPWRPRAQGTPATHIASEGLPQANPAAQSLPLDLKNAVAEYEIN
CCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHH
LLKQAMQQSQYNQRKAAQLLALSYHQFRGMLRKYQLLDSDNDAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STATESLLGESNAFLEIIEQASRLAPLRKPVLIIGERGTGKELIAHRLHYLSGRWDQSF
CCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCE
VTINCATLSESLLETELFGHEAGSFTGAAKRHQGRFERADGGTLFLDELATTSARVQEKL
EEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
LRVIEYGEFERVGGAKALKVDVRLVCATNEDLPALADKGHFRHDLLDRLAFDVITLPPLR
HHHHHCCCHHHCCCCEEEEEEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHH
ERREDILMLAEHFAHGMTRELGYPLFAGFSRKATQLLLDYPWPGNVRELKNVVERSIYRQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHC
GNPQLPLSDLVLNPFDSPWRPRAQGTPATHIASEGLPQANPAAQSLPLDLKNAVAEYEIN
CCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHH
LLKQAMQQSQYNQRKAAQLLALSYHQFRGMLRKYQLLDSDNDAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]