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The map label for this gene is mltF [H]

Identifier: 145299265

GI number: 145299265

Start: 2445773

End: 2447290

Strand: Direct

Name: mltF [H]

Synonym: ASA_2309

Alternate gene names: 145299265

Gene position: 2445773-2447290 (Clockwise)

Preceding gene: 145299264

Following gene: 145299266

Centisome position: 52.01

GC content: 57.84

Gene sequence:

>1518_bases
ATGAAAAGGTTATGGATCATTCTGGGCTGGATGCTGTGCTGCCTGGCGCCAGCCACGGCGGCTGAACAGACGGCAGCGCC
CGACGAAGTACCAGCGTCCCTGGCATTTAGCGCAGAGGAACTGTCCCGCCCTCGGTTGGGTGACCTCGATGACATGCTGG
CGCGACGCACCATCCGGGTGCTCACCACCTACAACAAGACCGGCTACTTCATCAACAAGGGGGTCCAACGGGGCGTAACC
TACGACGCTTTCATGCAGGTAGAAAAACGGCTCAACGATCAGCTGCGCAAACAGAAGGCCATCAAGCGCCACCTCAAGCT
GCAGTTCGTCTTCATTCCGGTTGCCCGTGAGGCCCTGCTGCAAGCCCTGATCGACGGCAAAGGTGACATAGCGGCAGCCA
ACCTGACCATCACGGATCAGCGCAAAAAGCAGGGCGTGGCGTTTACCAATCCCTTATTGGAAAACGTGCGGGAACTGCTG
ATCTCGGGGCCTCACTCACCCAGGGTGGAGAGTGTCGCCGAACTCGGCGGCCAAACTGTCCATGTGCGCCCCTCTTCAAG
TTACTTCGAGAGCCTCATCGCCTATAACCAGGCCCGCAAAGCCGCGGGAGAGCCGCTCATCGACATCAAGCCGGCACCGG
AATCTCTGGAAGATGAGGATCTGGTGGAGATGGTCAATGCTGGCCTGCTGCCCTTCATCGTCATGGATGAGCACAAGGCT
CGTTTCTGGAAGAAGATTTTTCCCGCCATCCAGATCCACGAACAACCCGCATTCCGTGAAGGGGGGGTCATCGCCTGGGC
GGTACGCAAGGAGAGTCCCAAGTTGCTCGCCATGCTCAACGAGCAGATAGCAGCCCAACGGGGCAAGGCCACAGGAGAGG
ACATCCTGGCCCGTTACCTCAAGCAGAACAAATTCGTCAAGAGCGCTGCCGCCGAGACTGAGCGCAAGAAATTCCTGCAG
CTGGTAGATCTGTTTCGCCAGTATGGCGATCGTTTCGATGTGGACTGGCTGCTGATCGCGGCTCAGGGCTATCAGGAGTC
GGCACTCAACCACAAGGCGCGCAGCCCAGTCGGTGCCATCGGCATCATGCAGATCATGCCCGCCACCGGCAAAGGTCTGA
AGGTAGGCGACATTCGCCAGCTCGAACCCAACATCCACGGAGGCGTCAAATACATGCGCTGGATGATCGATCACTACTAC
GCCGACGAGCCCATGACGGCCCTCGACAAGGCCTTGTTTTCATTTGCTTCCTACAATGCCGGCCCTGCACGGGTGGCACG
ACTGCGGACAGAGGCCAAGAAACGGGGACTGGATCCCAACATCTGGTTTCATAACGTCGAGTATGTGGCGGCCGAGAAAA
TAGGGCCAGACCCAGTGACCTACGTGGGCAACATCTACAAGTACTACATCGCCTACAAGCTGGTGATGGAGCAGCTGCAG
CTCAAACAGCAGGCCACTGAAACCATCAAACAGCAGGAGACCATCGGTGACAAGGTTTCCTCCGCGACAAAAACATAG

Upstream 100 bases:

>100_bases
TAGAGCCAATGGTGCTATCTGACACGTTGCACCAAACTCAATGAGCTAAGTAGCCCCTTGCCTGCCACTTACACTCAATG
AAAAGGGTGAGGTACTCATC

Downstream 100 bases:

>100_bases
CGGCGACCAAGCCGCTGCCAGCACGAACCAGGCTCCCCACTTTTTACGCGGTGAGCCTGATGTTTTTTACCTCCAGCTCC
TTGCCAACGCGACATCAGGC

Product: Slt family transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKRLWIILGWMLCCLAPATAAEQTAAPDEVPASLAFSAEELSRPRLGDLDDMLARRTIRVLTTYNKTGYFINKGVQRGVT
YDAFMQVEKRLNDQLRKQKAIKRHLKLQFVFIPVAREALLQALIDGKGDIAAANLTITDQRKKQGVAFTNPLLENVRELL
ISGPHSPRVESVAELGGQTVHVRPSSSYFESLIAYNQARKAAGEPLIDIKPAPESLEDEDLVEMVNAGLLPFIVMDEHKA
RFWKKIFPAIQIHEQPAFREGGVIAWAVRKESPKLLAMLNEQIAAQRGKATGEDILARYLKQNKFVKSAAAETERKKFLQ
LVDLFRQYGDRFDVDWLLIAAQGYQESALNHKARSPVGAIGIMQIMPATGKGLKVGDIRQLEPNIHGGVKYMRWMIDHYY
ADEPMTALDKALFSFASYNAGPARVARLRTEAKKRGLDPNIWFHNVEYVAAEKIGPDPVTYVGNIYKYYIAYKLVMEQLQ
LKQQATETIKQQETIGDKVSSATKT

Sequences:

>Translated_505_residues
MKRLWIILGWMLCCLAPATAAEQTAAPDEVPASLAFSAEELSRPRLGDLDDMLARRTIRVLTTYNKTGYFINKGVQRGVT
YDAFMQVEKRLNDQLRKQKAIKRHLKLQFVFIPVAREALLQALIDGKGDIAAANLTITDQRKKQGVAFTNPLLENVRELL
ISGPHSPRVESVAELGGQTVHVRPSSSYFESLIAYNQARKAAGEPLIDIKPAPESLEDEDLVEMVNAGLLPFIVMDEHKA
RFWKKIFPAIQIHEQPAFREGGVIAWAVRKESPKLLAMLNEQIAAQRGKATGEDILARYLKQNKFVKSAAAETERKKFLQ
LVDLFRQYGDRFDVDWLLIAAQGYQESALNHKARSPVGAIGIMQIMPATGKGLKVGDIRQLEPNIHGGVKYMRWMIDHYY
ADEPMTALDKALFSFASYNAGPARVARLRTEAKKRGLDPNIWFHNVEYVAAEKIGPDPVTYVGNIYKYYIAYKLVMEQLQ
LKQQATETIKQQETIGDKVSSATKT
>Mature_505_residues
MKRLWIILGWMLCCLAPATAAEQTAAPDEVPASLAFSAEELSRPRLGDLDDMLARRTIRVLTTYNKTGYFINKGVQRGVT
YDAFMQVEKRLNDQLRKQKAIKRHLKLQFVFIPVAREALLQALIDGKGDIAAANLTITDQRKKQGVAFTNPLLENVRELL
ISGPHSPRVESVAELGGQTVHVRPSSSYFESLIAYNQARKAAGEPLIDIKPAPESLEDEDLVEMVNAGLLPFIVMDEHKA
RFWKKIFPAIQIHEQPAFREGGVIAWAVRKESPKLLAMLNEQIAAQRGKATGEDILARYLKQNKFVKSAAAETERKKFLQ
LVDLFRQYGDRFDVDWLLIAAQGYQESALNHKARSPVGAIGIMQIMPATGKGLKVGDIRQLEPNIHGGVKYMRWMIDHYY
ADEPMTALDKALFSFASYNAGPARVARLRTEAKKRGLDPNIWFHNVEYVAAEKIGPDPVTYVGNIYKYYIAYKLVMEQLQ
LKQQATETIKQQETIGDKVSSATKT

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=486, Percent_Identity=25.1028806584362, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082441, Length=211, Percent_Identity=28.9099526066351, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 56771; Mature: 56771

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLWIILGWMLCCLAPATAAEQTAAPDEVPASLAFSAEELSRPRLGDLDDMLARRTIRV
CCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHEECHHHHCCCCCCCHHHHHHHHHHHH
LTTYNKTGYFINKGVQRGVTYDAFMQVEKRLNDQLRKQKAIKRHLKLQFVFIPVAREALL
HEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHH
QALIDGKGDIAAANLTITDQRKKQGVAFTNPLLENVRELLISGPHSPRVESVAELGGQTV
HHHHCCCCCEEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
HVRPSSSYFESLIAYNQARKAAGEPLIDIKPAPESLEDEDLVEMVNAGLLPFIVMDEHKA
EECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHH
RFWKKIFPAIQIHEQPAFREGGVIAWAVRKESPKLLAMLNEQIAAQRGKATGEDILARYL
HHHHHHCCHHEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
KQNKFVKSAAAETERKKFLQLVDLFRQYGDRFDVDWLLIAAQGYQESALNHKARSPVGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCHH
GIMQIMPATGKGLKVGDIRQLEPNIHGGVKYMRWMIDHYYADEPMTALDKALFSFASYNA
HHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GPARVARLRTEAKKRGLDPNIWFHNVEYVAAEKIGPDPVTYVGNIYKYYIAYKLVMEQLQ
CHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKQQATETIKQQETIGDKVSSATKT
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRLWIILGWMLCCLAPATAAEQTAAPDEVPASLAFSAEELSRPRLGDLDDMLARRTIRV
CCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHEECHHHHCCCCCCCHHHHHHHHHHHH
LTTYNKTGYFINKGVQRGVTYDAFMQVEKRLNDQLRKQKAIKRHLKLQFVFIPVAREALL
HEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHH
QALIDGKGDIAAANLTITDQRKKQGVAFTNPLLENVRELLISGPHSPRVESVAELGGQTV
HHHHCCCCCEEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
HVRPSSSYFESLIAYNQARKAAGEPLIDIKPAPESLEDEDLVEMVNAGLLPFIVMDEHKA
EECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHH
RFWKKIFPAIQIHEQPAFREGGVIAWAVRKESPKLLAMLNEQIAAQRGKATGEDILARYL
HHHHHHCCHHEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
KQNKFVKSAAAETERKKFLQLVDLFRQYGDRFDVDWLLIAAQGYQESALNHKARSPVGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCHH
GIMQIMPATGKGLKVGDIRQLEPNIHGGVKYMRWMIDHYYADEPMTALDKALFSFASYNA
HHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GPARVARLRTEAKKRGLDPNIWFHNVEYVAAEKIGPDPVTYVGNIYKYYIAYKLVMEQLQ
CHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKQQATETIKQQETIGDKVSSATKT
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA