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The map label for this gene is gldA [H]

Identifier: 145299232

GI number: 145299232

Start: 2400500

End: 2401618

Strand: Direct

Name: gldA [H]

Synonym: ASA_2274

Alternate gene names: 145299232

Gene position: 2400500-2401618 (Clockwise)

Preceding gene: 145299231

Following gene: 145299233

Centisome position: 51.05

GC content: 61.3

Gene sequence:

>1119_bases
ATGCCTATTCTGCCTCGTACCGTCACCTCCCCCAAAAAGTTCATCATAGGTCACGACCTTCTGCCTCAACTCCATGATCA
TGTGAAAGATTTTGGCGACAACGCCCTGCTCGTCAGCGATGAGTTCATCCTGGAGCGGGTGCGCGAAGAGGCGCTGGCCG
GTCTGCTGCAAGCAGGCTTGAAAGGACGGGTCGAGAAGTTCAACTACGAATGCACCGATATCGAGATCCGTCGCCTCTGC
GATCTCGCCGAACAGCAGGGCGCCAACGTGATCGTCGGTATCGGCGGCGGCAAGACCCTGGATGTGGCCAAGGCGGTTGC
CTTCTACCTGCGCCGTCCCGTTGTGCTGTTCCCCACCATTGCCTCCACCGATGCGCCCTGTACCGCACTGGCGGTGATTT
ACAATCAGGCGGGCGAGTTCGAGCGCTACCTGTTCCTCCCCCAGAACCCGGACGTGGTGATCGCCGATACCGCCATCATA
GCGGCGGCCCCTGCCCGCTTCTTCGCCGCTGGCGTGGGGGATGCCCTGGCCACCTATTTCGAGGCCCGCGCCTGCTATCG
GGCCGATGGCATCAACCTGGTGGGCAAGCGCCCCTCCCGTACCGGACTTGGCCTGGCAAAACTCTGCTATGAGCTGCTGG
GGGAAAACATAGAAATGGCGATGGATGCCATTCACCATCATGTCACCACACCGGCGCTCGAGCAGATCATTGAAGCCACC
ATCTACCTGAGTGGCGTCGGCGCCGAGGCCGGTGGTCTGGCGGCGGCACATGCGGTCAACAACGGCATGTCGGTGGTACC
TGACTTGCACCGTGCACAGCACGGGGAGAAGGTGGTATTCGGTCTGCTGACCCAGCTGGTGCTGGAGCGTGCGCCCCAGA
CCGAGGTCGATGAGGTGATGCGAATCATTCAGGTTGCCGGTCTGCCCATGACCTTGCAGGAGATGGGGCTGACCCATTTC
ATCGAGAGCGAGTGGCGCAAGGTGGCAGCGCTTGCCTGCGATCCCCTCGACACCATGGTCAATATGCCGATGGCCGTGAG
CGAGCAGGATGTTTACAACGCCATGATAGCGGCCAACGCCATGGCCGAGCGCTATCGGGCCCGTTATCCGCGGGATTGA

Upstream 100 bases:

>100_bases
CCATTACCCCTTCACCTGATGGCGAGCGAGGCGTATGGTGTGGCAGGAAGACCCGTTTGCATACCTAACGGGTGTCACTC
ACTGCCTTGAGGAATCCTGC

Downstream 100 bases:

>100_bases
TCCGGGATAAAACTCCTTGATGATCGACAACGAACAAGGCCAGCCTGACGGCTGGCCTTGTTCGTTGTGGTCTGAACTGC
TGAAATGGCGCTTCCCCCGT

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MPILPRTVTSPKKFIIGHDLLPQLHDHVKDFGDNALLVSDEFILERVREEALAGLLQAGLKGRVEKFNYECTDIEIRRLC
DLAEQQGANVIVGIGGGKTLDVAKAVAFYLRRPVVLFPTIASTDAPCTALAVIYNQAGEFERYLFLPQNPDVVIADTAII
AAAPARFFAAGVGDALATYFEARACYRADGINLVGKRPSRTGLGLAKLCYELLGENIEMAMDAIHHHVTTPALEQIIEAT
IYLSGVGAEAGGLAAAHAVNNGMSVVPDLHRAQHGEKVVFGLLTQLVLERAPQTEVDEVMRIIQVAGLPMTLQEMGLTHF
IESEWRKVAALACDPLDTMVNMPMAVSEQDVYNAMIAANAMAERYRARYPRD

Sequences:

>Translated_372_residues
MPILPRTVTSPKKFIIGHDLLPQLHDHVKDFGDNALLVSDEFILERVREEALAGLLQAGLKGRVEKFNYECTDIEIRRLC
DLAEQQGANVIVGIGGGKTLDVAKAVAFYLRRPVVLFPTIASTDAPCTALAVIYNQAGEFERYLFLPQNPDVVIADTAII
AAAPARFFAAGVGDALATYFEARACYRADGINLVGKRPSRTGLGLAKLCYELLGENIEMAMDAIHHHVTTPALEQIIEAT
IYLSGVGAEAGGLAAAHAVNNGMSVVPDLHRAQHGEKVVFGLLTQLVLERAPQTEVDEVMRIIQVAGLPMTLQEMGLTHF
IESEWRKVAALACDPLDTMVNMPMAVSEQDVYNAMIAANAMAERYRARYPRD
>Mature_371_residues
PILPRTVTSPKKFIIGHDLLPQLHDHVKDFGDNALLVSDEFILERVREEALAGLLQAGLKGRVEKFNYECTDIEIRRLCD
LAEQQGANVIVGIGGGKTLDVAKAVAFYLRRPVVLFPTIASTDAPCTALAVIYNQAGEFERYLFLPQNPDVVIADTAIIA
AAPARFFAAGVGDALATYFEARACYRADGINLVGKRPSRTGLGLAKLCYELLGENIEMAMDAIHHHVTTPALEQIIEATI
YLSGVGAEAGGLAAAHAVNNGMSVVPDLHRAQHGEKVVFGLLTQLVLERAPQTEVDEVMRIIQVAGLPMTLQEMGLTHFI
ESEWRKVAALACDPLDTMVNMPMAVSEQDVYNAMIAANAMAERYRARYPRD

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=366, Percent_Identity=47.5409836065574, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1786815, Length=383, Percent_Identity=29.242819843342, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 40536; Mature: 40405

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00913 ADH_IRON_1 ; PS00687 ALDEHYDE_DEHYDR_GLU ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILPRTVTSPKKFIIGHDLLPQLHDHVKDFGDNALLVSDEFILERVREEALAGLLQAGL
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
KGRVEKFNYECTDIEIRRLCDLAEQQGANVIVGIGGGKTLDVAKAVAFYLRRPVVLFPTI
HCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEC
ASTDAPCTALAVIYNQAGEFERYLFLPQNPDVVIADTAIIAAAPARFFAAGVGDALATYF
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
EARACYRADGINLVGKRPSRTGLGLAKLCYELLGENIEMAMDAIHHHVTTPALEQIIEAT
HHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
IYLSGVGAEAGGLAAAHAVNNGMSVVPDLHRAQHGEKVVFGLLTQLVLERAPQTEVDEVM
HHEEECCCCCCCHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
RIIQVAGLPMTLQEMGLTHFIESEWRKVAALACDPLDTMVNMPMAVSEQDVYNAMIAANA
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHH
MAERYRARYPRD
HHHHHHHCCCCC
>Mature Secondary Structure 
PILPRTVTSPKKFIIGHDLLPQLHDHVKDFGDNALLVSDEFILERVREEALAGLLQAGL
CCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
KGRVEKFNYECTDIEIRRLCDLAEQQGANVIVGIGGGKTLDVAKAVAFYLRRPVVLFPTI
HCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEC
ASTDAPCTALAVIYNQAGEFERYLFLPQNPDVVIADTAIIAAAPARFFAAGVGDALATYF
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
EARACYRADGINLVGKRPSRTGLGLAKLCYELLGENIEMAMDAIHHHVTTPALEQIIEAT
HHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
IYLSGVGAEAGGLAAAHAVNNGMSVVPDLHRAQHGEKVVFGLLTQLVLERAPQTEVDEVM
HHEEECCCCCCCHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
RIIQVAGLPMTLQEMGLTHFIESEWRKVAALACDPLDTMVNMPMAVSEQDVYNAMIAANA
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHH
MAERYRARYPRD
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]