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The map label for this gene is cca [H]

Identifier: 145297078

GI number: 145297078

Start: 3295399

End: 3296811

Strand: Reverse

Name: cca [H]

Synonym: cgR_2974

Alternate gene names: 145297078

Gene position: 3296811-3295399 (Counterclockwise)

Preceding gene: 145297080

Following gene: 145297077

Centisome position: 99.48

GC content: 53.29

Gene sequence:

>1413_bases
ATGGCGCGCGCTCACCAGGCAATTGGTGAGCTTTCAGACATCCTTGTGCCCCTAGCGGCAGCATTTTCCAGCAAGGGACA
CTCCCTATATTTGGTGGGCGGTTCCGTGAGGGATGCTTTCTTAGGTGAGTTGGGGCATGACCTTGATTTCACCACGTCGG
CACGCCCTGAGGAGACAAAGGCGATCCTGGATGATTACGCCGATGTGGTGTGGGATACCGGAATTGCGTTTGGCACCCTT
TCTGCAGAAAAGCATGGTCAGCAGATCGAAATCACGACATTCCGTTCTGATCTCTATGACGGAAACTCCCGCAACCCTGA
AGTGACCTTCGGTGACACCTTGGAAGGCGATCTGATTAGGCGCGATTTCAAGGTCAACGCCATGGCTGCGGAGATCCAAG
CCGATGGTGAATTAACTTTCCACGATCCAGTGGGTGGCTTGGAAGATCTACTTACCCACACCTTGGATACCCCAGCTACT
CCGGAACAATCATTCAATGATGATCCTCTGCGAATGCTGCGCGCTGCCCGCTTTGTTTCCCAACTGAATTTCACCCTTGC
ACCACGAGTTATTACCGCCATGACTGAAATGGCGCAGCAGATTACTCGGATCACTGTGGAACGCATGCAGGTTGAGTTGG
ACAAAATGATCCTTGGCAAAAACCCTGAGGCTGGCATTGACCTCATGGTGGAGTCCGGTATCGCGCAGATTGTTTATCCG
GAGATCCCTGCGATGCAGATGACTCAAGATGAACACATGCAGCACAAGGATGTGTACGCGCACTCGTTGCAGGTGATGCG
CCAAGCGATTGATCAGGAAGAAGATGGCCCTGACCTGGTGCTTCGTTGGGCTGCACTGCTTCATGATTGCGGTAAGCCAG
ATACTCGTGACTTCAATGAGGAAGGCCGCGTGAGCTTCCATCAGCATGAGGTAGTTGGCGCCAAGCTGGTGAGGCGACGG
ATGCGCAAGCTGAAGTACTCAAAGCAAATGGTCGGCGATGTCGGGCAGTTGGTGTTCCTGCACATGCGTTTCCATGGTTT
CAGCGAAGGCCAGTGGACGGATTCTGCAGTGCGTAGGTATGCCGCAGATGCGGGTGAATTGCTGCCACGTTTGCACAAGT
TAGTGCGTGCTGATTGCACCACAAGAAATAAAAGGAAGGCAGCACGACTGCAAGCCACCTACGATCATTTGGAAGAGCGC
ATCGCGGAGATCGCCGCAAAGGAAGATCTTGCCAGGGTGCGCCCAGATTTGGATGGCAATGAGATCATGGAGATTCTGAA
CATCCAAGCCGGACCTGAAGTGGGTAAGGCGTGGGCGTTTTTGAAGGAGCTTCGTTTGGAGCGCGGTCCTTTGGATCGTG
AAGTTGCCATCGCAGAGCTGAAGAGCTGGTGGGAAGGAGAAAACAATGAGTGA

Upstream 100 bases:

>100_bases
CACACTTCTACTGGGTAGACTCAGATTCGTGAATTCCCAGGATGCTCAGAACCAAGTTCCCCCACAGAAAGTGGACCAGG
AAGTAGACCGCCTTGCTTTA

Downstream 100 bases:

>100_bases
TTTTTATGCCGACAGGTTGTTTAATGCGATGGAGCGCAATGAGGTAGCTCCGGGCATGTTGTTGGTGGCTGCGCCCGATA
TGGCGTCGGAGGATTTTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARPEETKAILDDYADVVWDTGIAFGTL
SAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAAEIQADGELTFHDPVGGLEDLLTHTLDTPAT
PEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKMILGKNPEAGIDLMVESGIAQIVYP
EIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTRDFNEEGRVSFHQHEVVGAKLVRRR
MRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVRADCTTRNKRKAARLQATYDHLEER
IAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVAIAELKSWWEGENNE

Sequences:

>Translated_470_residues
MARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARPEETKAILDDYADVVWDTGIAFGTL
SAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAAEIQADGELTFHDPVGGLEDLLTHTLDTPAT
PEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKMILGKNPEAGIDLMVESGIAQIVYP
EIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTRDFNEEGRVSFHQHEVVGAKLVRRR
MRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVRADCTTRNKRKAARLQATYDHLEER
IAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVAIAELKSWWEGENNE
>Mature_469_residues
ARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARPEETKAILDDYADVVWDTGIAFGTLS
AEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAAEIQADGELTFHDPVGGLEDLLTHTLDTPATP
EQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKMILGKNPEAGIDLMVESGIAQIVYPE
IPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTRDFNEEGRVSFHQHEVVGAKLVRRRM
RKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVRADCTTRNKRKAARLQATYDHLEERI
AEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVAIAELKSWWEGENNE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=234, Percent_Identity=31.1965811965812, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789436, Length=335, Percent_Identity=30.4477611940298, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081691, Length=257, Percent_Identity=27.2373540856031, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=221, Percent_Identity=25.7918552036199, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=30.9012875536481, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI281360112, Length=294, Percent_Identity=26.8707482993197, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357337, Length=294, Percent_Identity=26.8707482993197, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52893; Mature: 52761

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARPEETK
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHH
AILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIR
HHHHHHHHHHHHCCCCEEECCHHHCCCEEEEEEEHHHHCCCCCCCCCEEECCCCCCHHHH
RDFKVNAMAAEIQADGELTFHDPVGGLEDLLTHTLDTPATPEQSFNDDPLRMLRAARFVS
HHHHHHHHHEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
QLNFTLAPRVITAMTEMAQQITRITVERMQVELDKMILGKNPEAGIDLMVESGIAQIVYP
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHEECC
EIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTRDFNE
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
EGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRY
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
AADAGELLPRLHKLVRADCTTRNKRKAARLQATYDHLEERIAEIAAKEDLARVRPDLDGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVAIAELKSWWEGENNE
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARPEETK
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHH
AILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIR
HHHHHHHHHHHHCCCCEEECCHHHCCCEEEEEEEHHHHCCCCCCCCCEEECCCCCCHHHH
RDFKVNAMAAEIQADGELTFHDPVGGLEDLLTHTLDTPATPEQSFNDDPLRMLRAARFVS
HHHHHHHHHEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
QLNFTLAPRVITAMTEMAQQITRITVERMQVELDKMILGKNPEAGIDLMVESGIAQIVYP
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHEECC
EIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTRDFNE
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
EGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRY
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
AADAGELLPRLHKLVRADCTTRNKRKAARLQATYDHLEERIAEIAAKEDLARVRPDLDGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVAIAELKSWWEGENNE
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA