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The map label for this gene is trpG [H]

Identifier: 145297020

GI number: 145297020

Start: 3237929

End: 3238555

Strand: Direct

Name: trpG [H]

Synonym: cgR_2917

Alternate gene names: 145297020

Gene position: 3237929-3238555 (Clockwise)

Preceding gene: 145297019

Following gene: 145297021

Centisome position: 97.7

GC content: 54.23

Gene sequence:

>627_bases
ATGACACACGTTGTTCTCATTGATAATCACGATTCTTTTGTCTACAACCTGGTGGATGCGTTCGCCGTGGCCGGTTATAA
GTGCACGGTGTTCCGCAATACGGTGCCAGTTGAAACCATTTTGGCAGCCAACCCGGACCTAATCTGCCTTTCACCTGGAC
CTGGTTACCCTGCCGATGCGGGCAACATGATGGCGCTGATCGAGCGCACACTCAGCCAGATTCCTTTACTGGGTATTTGC
CTCGGCTACCAGGCGCTCATCGAATACCACGGCGGCAAGGTTGAGCCTTGTGGCCCTGTGCACGGCACCACCGACAACAT
GATCCTTACTGATGCAGGTGTGCAGAGCCCTGTTTTTGCAGGTCTTGCCACTGATGTTGAGCCTGATCATCCAGAAATCC
CAGGCCGCAAGGTTCCAATTGGCCGTTATCACTCACTGGGCTGCGTGGTTGCCCCAGACGGTATTGAATCACTAGGTACC
TGTTCCTCGGAGATTGGTGATGTCATCATGGCGGCACGCACCACCGATGGAAAGGCCATTGGACTGCAGTTTCACCCTGA
GTCAGTGCTGAGCCCAACGGGTCCTGTCATTTTGTCCCGCTGTGTCGAACAACTTCTCGCGAACTAA

Upstream 100 bases:

>100_bases
ATTCTAATCCTCAATCTGAAGCCGATGAGACGTTGCACAAGGCGTATGCCGTGTTGAATGCCATTGCGCTTGCTGCTGGT
TCCACTTTGGAGGTCATCCG

Downstream 100 bases:

>100_bases
TTAAAAAAGGATTTGATTCATGACTTCTCCAGCAACACTGAAAGTTCTCAACGCCTACTTGGATAACCCCACTCCAACCC
TGGAGGAGGCAATTGAGGTG

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Anthranilate synthase component II; Glutamine amido-transferase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTHVVLIDNHDSFVYNLVDAFAVAGYKCTVFRNTVPVETILAANPDLICLSPGPGYPADAGNMMALIERTLSQIPLLGIC
LGYQALIEYHGGKVEPCGPVHGTTDNMILTDAGVQSPVFAGLATDVEPDHPEIPGRKVPIGRYHSLGCVVAPDGIESLGT
CSSEIGDVIMAARTTDGKAIGLQFHPESVLSPTGPVILSRCVEQLLAN

Sequences:

>Translated_208_residues
MTHVVLIDNHDSFVYNLVDAFAVAGYKCTVFRNTVPVETILAANPDLICLSPGPGYPADAGNMMALIERTLSQIPLLGIC
LGYQALIEYHGGKVEPCGPVHGTTDNMILTDAGVQSPVFAGLATDVEPDHPEIPGRKVPIGRYHSLGCVVAPDGIESLGT
CSSEIGDVIMAARTTDGKAIGLQFHPESVLSPTGPVILSRCVEQLLAN
>Mature_207_residues
THVVLIDNHDSFVYNLVDAFAVAGYKCTVFRNTVPVETILAANPDLICLSPGPGYPADAGNMMALIERTLSQIPLLGICL
GYQALIEYHGGKVEPCGPVHGTTDNMILTDAGVQSPVFAGLATDVEPDHPEIPGRKVPIGRYHSLGCVVAPDGIESLGTC
SSEIGDVIMAARTTDGKAIGLQFHPESVLSPTGPVILSRCVEQLLAN

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=212, Percent_Identity=35.377358490566, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1789760, Length=209, Percent_Identity=37.3205741626794, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322638, Length=204, Percent_Identity=35.2941176470588, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324361, Length=220, Percent_Identity=29.5454545454545, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21905; Mature: 21774

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVVLIDNHDSFVYNLVDAFAVAGYKCTVFRNTVPVETILAANPDLICLSPGPGYPADA
CEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCHHHEEECCCCEEEECCCCCCCCCC
GNMMALIERTLSQIPLLGICLGYQALIEYHGGKVEPCGPVHGTTDNMILTDAGVQSPVFA
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHH
GLATDVEPDHPEIPGRKVPIGRYHSLGCVVAPDGIESLGTCSSEIGDVIMAARTTDGKAI
HEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCHHHHHHEEEEEECCCCCEE
GLQFHPESVLSPTGPVILSRCVEQLLAN
EEEECCHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
THVVLIDNHDSFVYNLVDAFAVAGYKCTVFRNTVPVETILAANPDLICLSPGPGYPADA
EEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCHHHEEECCCCEEEECCCCCCCCCC
GNMMALIERTLSQIPLLGICLGYQALIEYHGGKVEPCGPVHGTTDNMILTDAGVQSPVFA
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHH
GLATDVEPDHPEIPGRKVPIGRYHSLGCVVAPDGIESLGTCSSEIGDVIMAARTTDGKAI
HEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCHHHHHHEEEEEECCCCCEE
GLQFHPESVLSPTGPVILSRCVEQLLAN
EEEECCHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3808947; 12948626 [H]